公开(公告)号：WO2013070627A3

公开(公告)日：2013-08-22

申请号：PCT/US2012063741

申请日：2012-11-06

Applicant: ILLUMINA INC

Inventor： SHEN MIN-JUI RICHARD ,  KAIN ROBERT C ,  KUHN KENNETH M ,  TALASAZ AMIRALI HAJHOSSEIN ,  JAMSHIDI ARASH ,  SAKALDASIS GEORGE ,  VERMAAS ERIC ,  BOHM SEBASTIAN ,  KHURANA TARUN ,  ELTOUKHY HELMY A ,  GONG JIAN

IPC: B01L3/00

CPC classification number: C12Q1/6874 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0851 ,  B01L2300/165 ,  B01L2400/0427

Abstract: Provided are methods and apparatuses for performing sequencing using droplet manipulation, for example, via electrowetting-based techniques. Also provided are integrated methods and apparatuses for performing sample preparation and sequencing on the same apparatus. In addition, provided are methods of reducing reagent waste and preloaded consumable cartridges comprising reagents for sample preparation and/or sequencing.

Abstract translation: 提供了用于使用液滴操纵进行排序的方法和装置，例如通过基于电润湿的技术。 还提供了用于在同一装置上进行样品制备和测序的综合方法和装置。 此外，提供了减少试剂废物的方法和包括用于样品制备和/或测序的试剂的预装的消耗品筒。

公开(公告)号：WO2015187868A3

公开(公告)日：2016-01-28

申请号：PCT/US2015034053

申请日：2015-06-03

Applicant: ILLUMINA INC

Inventor： BOHM SEBASTIAN ,  ARAVANIS ALEX ,  HSIAO ALEXANDER ,  JAVANMARDI BEHNAM ,  KHURANA TARUN ,  TRAN HAI QUANG ,  AGHABABAZADEH MAJID ,  BOWEN M SHANE ,  BOYANOV BOYAN ,  BUERMANN DALE

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  C12Q1/6869 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

Abstract translation: 用于进行指定反应的系统和方法，其包括具有样品通道的流体网络，反应室和储存器。 样品通道与样品端口流通。 该系统还包括具有流动通道并被配置为在第一和第二阀位置之间旋转的旋转阀。 当旋转阀处于第一阀位置时，流动通道流体耦合反应室和样品通道，并且当旋转阀处于第二阀位置时，流体流动地联接储存器和反应室。 当旋转阀处于第一阀位置时，泵组件将生物样品流向反应室，当旋转阀处于第二阀位置时，泵组件将反应组分从储存器流向反应室。

公开(公告)号：WO2015187868A8

公开(公告)日：2017-01-05

申请号：PCT/US2015034053

申请日：2015-06-03

Applicant: ILLUMINA INC

Inventor： BOHM SEBASTIAN ,  ARAVANIS ALEX ,  HSIAO ALEXANDER ,  JAVANMARDI BEHNAM ,  KHURANA TARUN ,  TRAN HAI QUANG ,  AGHABABAZADEH MAJID ,  BOWEN M SHANE ,  BOYANOV BOYAN ,  BUERMANN DALE

IPC: B01L3/00

CPC classification number: B01L3/502738 ,  B01L3/502715 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/0655 ,  C12Q1/6869 ,  F16K99/0013 ,  F16K99/0015 ,  F16K99/0028 ,  F16K2099/0084

Abstract: Systems and methods for conducting designated reactions that include a fluidic network having a sample channel, a reaction chamber, and a reservoir. The sample channel is in flow communication with a sample port. The system also includes a rotary valve that has a flow channel and is configured to rotate between first and second valve positions. The flow channel fluidically couples the reaction chamber and the sample channel when the rotary valve is in the first valve position and fluidically couples the reservoir and the reaction chamber when the rotary valve is in the second valve position. A pump assembly induces a flow of a biological sample toward the reaction chamber when the rotary valve is in the first valve position and induces a flow of a reaction component from the reservoir toward the reaction chamber when the rotary valve is in the second valve position.

Abstract translation: 用于进行指定反应的系统和方法，其包括具有样品通道的流体网络，反应室和储存器。 样品通道与样品端口流通。 该系统还包括具有流动通道并被配置为在第一和第二阀位置之间旋转的旋转阀。 当旋转阀处于第一阀位置时，流动通道流体耦合反应室和样品通道，并且当旋转阀处于第二阀位置时，流体流动地联接储存器和反应室。 当旋转阀处于第一阀位置时，泵组件将生物样品流向反应室，当旋转阀处于第二阀位置时，泵组件将反应组分从储存器流向反应室。