公开(公告)号：WO2015186004A3

公开(公告)日：2016-05-12

申请号：PCT/IB2015001643

申请日：2015-06-02

Applicant: LAB FRANCAIS DU FRACTIONNEMENT

Inventor： MASIELLO NICHOLAS C

IPC: C07K14/715 ,  C07K16/04 ,  C07K16/32

CPC classification number: C07K16/32 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2227/10 ,  A01K2267/01 ,  B01D15/30 ,  B01D15/3809 ,  B01D15/426 ,  B01D61/145 ,  B01D2311/2626 ,  C07K16/04 ,  C07K2317/12 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2317/76 ,  C07K2317/92 ,  C12P21/02 ,  C12Y204/99 ,  C12Y304/22002

Abstract: In one aspect, the disclosure provides cells and transgenic non-human mammals for the production of Fc fragments, as well as compositions and uses thereof.

Abstract translation: 一方面，本公开提供用于产生Fc片段的细胞和转基因非人哺乳动物，以及其组合物和用途。

公开(公告)号：WO00058722A1

公开(公告)日：2000-10-05

申请号：PCT/FR2000/000830

申请日：2000-03-31

IPC: B01D15/18 ,  B01D15/22 ,  B01D15/30 ,  G01N30/42

CPC classification number: B01D15/1892 ,  B01D15/22 ,  B01D15/30 ,  G01N30/42 ,  G01N30/6091

Abstract: A centrifuge partition chromatography device for separating a liquid into at least two phases, comprising at least one flat ring (3a) that can be rotationally driven around its axis, disposed in a substantially vertical position. Said ring (3a) comprises a plurality of cells (7) whose dimensions are greater in a first direction than the dimensions in a second direction which is substantially perpendicular to the first, disposed side by side and distributed along the periphery of said ring (3a) in addition to being connected to each other in a series by inlet and outlet ducts (15) terminating in the opposite respective ends of each cells (7) enabling the circulation of liquid. The invention is characterized in that said ducts (15) are disposed in such a way that the liquid supply jet penetrates the cells (7) in a direction that includes at least one radial component relative to the axis of rotation of said ring (3a).

Abstract translation: 一种用于将液体分离成至少两相的离心机分配色谱装置，包括至少一个围绕其轴旋转地驱动的平环（3a），所述平环设置在基本垂直的位置。 所述环（3a）包括多个单元（7），其尺寸在第一方向上大于沿着第二方向的尺寸，该第二方向基本上垂直于第一方向并排设置，并且沿着所述环（3a）的周边分布 ）除了通过入口和出口管道（15）彼此连接之外，终止于每个电池单元（7）的相对的各个端部，使得能够循环液体。 本发明的特征在于，所述管道（15）以这样的方式设置，使得液体供应射流沿着包括相对于所述环（3a）的旋转轴线的至少一个径向分量的方向穿透细胞（7） 。

公开(公告)号：WO1985003885A1

公开(公告)日：1985-09-12

申请号：PCT/EP1985000054

申请日：1985-02-20

Applicant: MERCK PATENT GESELLSCHAFT MIT BESCHRÄNKTER ... ,  MÜLLER, Werner ,  SÄNGER, Heinz, Ludwig

Inventor： MERCK PATENT GESELLSCHAFT MIT BESCHRÄNKTER ...

IPC: B01J20/32

CPC classification number: B01J20/327 ,  B01D15/30 ,  B01J20/28004 ,  B01J20/281 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3278 ,  B01J20/3282 ,  B01J20/3287 ,  B01J20/3289 ,  B01J2220/54 ,  B01J2220/58 ,  Y10S435/803

Abstract: Phase supports for the partition chromatography of macromolecules from non absorbing base support particles, insoluble in the phase system, with an average particulate size from 7 to 2000 mum , of which the surface is coated with an adhering material which is insoluble in the phase system and presenting an affinity for one of the phases of the phase system for the partition chromatography; their production method and their utilization for the separation by partition chromatography of macromolecules, biopolymers, subcellular units and complete cells, particularly in the polyethylene glycol-dextrane-dual phase system.

公开(公告)号：WO2013134251A3

公开(公告)日：2015-07-02

申请号：PCT/US2013029097

申请日：2013-03-05

Applicant: BIO RAD LABORATORIES

Inventor： LIAO JIALI ,  FROST RUSSELL

IPC: B01D15/08 ,  B01J20/26

CPC classification number: B01J41/20 ,  B01D15/265 ,  B01D15/30 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3847 ,  B01J20/22 ,  B01J20/267 ,  B01J20/28016 ,  B01J20/281 ,  B01J20/286 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3208 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3253 ,  B01J20/3265 ,  B01J20/3293 ,  B01J41/04 ,  B01J41/09 ,  B01J2220/52 ,  C07K1/14 ,  C07K1/16 ,  C07K1/165 ,  C07K16/00 ,  G01N30/482

Abstract: Anion exchange-hydrophobic mixed mode ligands and methods of their use are provided.

Abstract translation: 提供阴离子交换疏水混合模式配体及其使用方法。

公开(公告)号：WO2013173501A2

公开(公告)日：2013-11-21

申请号：PCT/US2013/041221

申请日：2013-05-15

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： WYNDHAM, Kevin, D. ,  MORRIS, Michael, F. ,  BROUSMICHE, Darryl, W. ,  HILL, Jason, F. ,  FAIRCHILD, Jacob, N.

IPC: B01J20/22

CPC classification number: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3234 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  B01J2220/54 ,  B01J2220/80 ,  C07F7/1836

Abstract: In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1:[X](W) a (Q) b (T) c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05 ≤ (b/c) ≤ 100, and a ≥ 0.

Abstract translation: 一方面，本发明提供了用于由式1表示的各种不同色谱方式的色谱固定相材料：[X]（W）a（Q）b（T）c（式1）。 X可以是具有包含二氧化硅芯材料，金属氧化物芯材料，无机 - 有机杂化材料或其一组嵌段共聚物的表面的高纯度色谱芯组合物。 W可以不存在和/或可以包括氢和/或可以在X的表面上包括羟基.Q可以是在使用低水浓度的色谱条件下最小化随时间（漂移）的保留变化的官能团。 T可以包括与分析物色谱相互作用的一个或多个亲水性，极性的，可离子化的和/或带电的官能团。 另外，b和c可以是正数，0.05 <=（b / c）<= 100，a> = 0。

公开(公告)号：WO2013076049A1

公开(公告)日：2013-05-30

申请号：PCT/EP2012/073027

申请日：2012-11-20

Applicant: GLAXO GROUP LIMITED ,  PINK, Christopher ,  RUNDQUIST, Elin

Inventor： PINK, Christopher ,  RUNDQUIST, Elin

IPC: B01D15/12 ,  B01D15/18 ,  B01D15/24 ,  B01D15/30 ,  G01N30/06 ,  G01N30/14 ,  G01N30/84 ,  B01D61/02

CPC classification number: B01D11/0415 ,  B01D15/12 ,  B01D15/1807 ,  B01D15/24 ,  B01D15/30 ,  B01D61/027 ,  B01D2011/002 ,  B01D2311/2626 ,  G01N30/30 ,  G01N2030/027 ,  G01N2030/3084

Abstract: A process for separating a compound from solution in organic solvent by a chromatographic process, in particular liquid-liquid chromatography, in which prior to the chromatographic process the composition of the solution is changed by means of a process of organic solvent nanofiltration for a solvent exchange. Additionally or alternatively subsequent to the chromatographic process the output from the chromatographic process is subjected to a process of organic solvent nanofiltration to remove residual target compound and/or impurities in the output for solvent recovery.

Abstract translation: 一种通过色谱法，特别是液相色谱法在有机溶剂中从溶液中分离化合物的方法，其中在色谱法之前，溶液的组成通过有机溶剂纳滤过程进行溶剂交换而改变 。 另外或替代地，在色谱过程之后，色谱过程的输出经过有机溶剂纳滤过程以除去输出中残留的目标化合物和/或杂质用于溶剂回收。

公开(公告)号：WO2006099308A2

公开(公告)日：2006-09-21

申请号：PCT/US2006008919

申请日：2006-03-10

Applicant: WYETH CORP ,  BROWN PAUL ,  COFFMAN JON ,  GODAVARTI RANGANATHAN ,  ISKRA TIM ,  KELLEY BRIAN D ,  VUNNUM SURESH ,  SUN SHUJUN ,  YU TIANNING ,  BOOTH JAMES EDWARD ,  SWITZER MARY BETH

Inventor： BROWN PAUL ,  COFFMAN JON ,  GODAVARTI RANGANATHAN ,  ISKRA TIM ,  KELLEY BRIAN D ,  VUNNUM SURESH ,  SUN SHUJUN ,  YU TIANNING ,  BOOTH JAMES EDWARD ,  SWITZER MARY BETH

IPC: A01H1/00

CPC classification number: B01D15/30 ,  B01D15/327 ,  B01D15/361 ,  B01D15/3804 ,  B01D15/3828 ,  C07K1/16 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22 ,  C07K16/065

Abstract: This invention relates to methods of using weak partitioning chromatography for the purification of a product from a load fluid containing one or more impurities. Further, the invention relates to methods of weak partitioning chromatography defined by operating conditions which cause a medium to bind least 1 mg of product per mL of medium, or alternatively, defined by a partition coefficient of at least 0.1.

Abstract translation: 本发明涉及使用弱分配色谱从含有一种或多种杂质的载体流体中纯化产物的方法。 此外，本发明涉及由分配系数至少为0.1的操作条件所限定的弱分配色谱法，该操作条件使培养基与每毫升培养基中的至少1mg产物结合，或者由分配系数限定。

公开(公告)号：WO2015189832A1

公开(公告)日：2015-12-17

申请号：PCT/IB2015/054494

申请日：2015-06-13

Applicant: LUPIN LIMITED

Inventor： BANERJEE, Abir ,  GANAPATHY, Chandranath ,  MODY, Sorab, Rustom ,  MISHRA, Ashok

IPC: C07K14/715

CPC classification number: C07K14/70578 ,  B01D15/12 ,  B01D15/203 ,  B01D15/30 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/3847 ,  B01D15/424 ,  C07K14/7151 ,  C07K2319/30

Abstract: The present invention relates to the purification of TNFR:Fc fusion protein. More specifically related to process of purification of TNFR:Fc fusion protein wherein the HCP is reduced. The present invention is directed to the use of mixed-mode chromatography and/or affinity chromatography to produce TNFR:Fc fusion protein which is substantially free of at least one of the protein degrading enzyme present in HCP.

Abstract translation: 本发明涉及TNFR：Fc融合蛋白的纯化。 更具体地涉及其中HCP被还原的TNFR：Fc融合蛋白的纯化方法。 本发明涉及使用混合模式色谱和/或亲和层析来制备基本上不含存在于HCP中的蛋白质降解酶中的至少一种的TNFR：Fc融合蛋白。

公开(公告)号：WO2015134317A8

公开(公告)日：2015-11-26

申请号：PCT/US2015017972

申请日：2015-02-27

Applicant: WATERS TECHNOLOGIES CORP

Inventor： BROUSMICHE DARRYL W ,  FAIRCHILD JACOB N ,  HILL JASON F ,  ISAAC GIORGIS ,  MORRIS MICHAEL F ,  WYNDHAM KEVIN D

IPC: B01D15/08 ,  B01D15/26 ,  B01J20/32

CPC classification number: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3234 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  B01J2220/54 ,  B01J2220/80 ,  C07F7/1836

Abstract: The present disclosure relates to a method of separating a compound of interest, particularly unsaturated compound(s) of interest, from a mixture. The compound is separated using a column having a chromatographic stationary phase material for various different modes of chromatography containing a first substituent and a second substituent. The first substituent minimizes compound retention variation over time under chromatographic conditions. The second substituent chromatographically and selectively retains the compound by incorporating one or more aromatic, polyaromatic, heterocyclic aromatic, or polyheterocyclic aromatic hydrocarbon groups, each group being optionally substituted with an aliphatic group.

Abstract translation: 本公开涉及将目的化合物，特别是目标的不饱和化合物与混合物分离的方法。 使用具有色谱固定相材料的色谱柱分离化合物，用于各种不同的含有第一取代基和第二取代基的不同色谱方式。 在色谱条件下，第一取代基使化合物保留时间变化最小化。 第二取代基通过引入一个或多个芳族，多芳族，杂环芳族或多杂环芳族烃基来色谱和选择性地保留化合物，每个基团任选被脂族基团取代。

公开(公告)号：WO2013173494A1

公开(公告)日：2013-11-21

申请号：PCT/US2013/041207

申请日：2013-05-15

Applicant: WATERS TECHNOLOGIES CORPORATION

Inventor： BROUSMICHE, Darryl, W. ,  WYNDHAM, Kevin, D. ,  FAIRCHILD, Jacob, N. ,  IRANETA, Pamela, C. ,  HILL, Jason, F.

IPC: B01D15/26 ,  B01J20/32

CPC classification number: B01J20/283 ,  B01D15/26 ,  B01D15/30 ,  B01D15/305 ,  B01D15/322 ,  B01D15/40 ,  B01D53/025 ,  B01J20/22 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/28042 ,  B01J20/28057 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28064 ,  B01J20/28066 ,  B01J20/28069 ,  B01J20/28071 ,  B01J20/28073 ,  B01J20/28076 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/282 ,  B01J20/284 ,  B01J20/285 ,  B01J20/288 ,  B01J20/289 ,  B01J20/3085 ,  B01J20/32 ,  B01J20/3204 ,  B01J20/3206 ,  B01J20/3208 ,  B01J20/3234 ,  B01J20/3246 ,  B01J20/3285 ,  B01J20/3293 ,  B01J2220/54 ,  B01J2220/80 ,  C07F7/1836

Abstract: The present invention provides a chromatographic stationary phase material various different types of chromatography. One example chromatographic stationary phase is represented by Formula 1 [X](W) a (Q) b (T) c (Formula 1). X can be a high purity chromatographic core composition. W can be absent and/or can include hydrogen and/or can include hydroxyl on the surface of X. Q can be bound directly to X and can include a first hydrophilic, polar, ionizable, and/or charged functional group that chromatographically interacts with the analyte. T can be bound directly to X and can include a second hydrophilic, polar, ionizable, and/or charged functional group that chromatographically interacts with the analyte. Additionally, Q and T can essentially eliminate chromatographic interaction between the analyte, and X and W, thereby minimizing retention variation over time (drift or change) under chromatographic conditions utilizing low water concentrations.

Abstract translation: 本发明提供了各种不同类型的色谱法的色谱固定相材料。 一个实例的色谱固定相由式1 [X]（W）a（Q）b（T）c（式1）表示。 X可以是高纯度色谱芯组成。 W可以不存在和/或可以包括氢和/或可以在X的表面上包括羟基.Q可以直接结合X，并且可以包括第一亲水，极性，可离子化和/或带电的官能团，其与色谱相互作用 分析物。 T可以直接与X结合，并且可以包括与分析物色谱相互作用的第二亲水，极性，可离子化和/或带电的官能团。 此外，Q和T基本上可以消除分析物与X和W之间的色谱相互作用，从而尽可能减少使用低水浓度的色谱条件下随时间的滞留变化（漂移或变化）。