公开(公告)号：WO2021055770A2

公开(公告)日：2021-03-25

申请号：PCT/US2020/051520

申请日：2020-09-18

Applicant: EXOSOME DIAGNOSTICS, INC.

Inventor： SKOG, Johan Karl Olov ,  OGUNLUSI, Olubode A. ,  KITCHEN, Robert Raymond ,  ROBERTS, Douglas ,  YU, Wei

IPC: G01N33/50 ,  C12N15/10 ,  G01N33/537 ,  G01N33/543 ,  C12N15/1013 ,  G01N33/5076 ,  G01N33/54393

Abstract: The present disclosure relates to compositions, methods and kits for the isolation of extracellular vesicles. The compositions, methods and kits can comprise a high-density liquid reagent that facilitates the sequestration of extracellular vesicles bound to magnetic beads.

公开(公告)号：WO2021198289A1

公开(公告)日：2021-10-07

申请号：PCT/EP2021/058352

申请日：2021-03-30

Applicant: UNIVERSITÉ DE LIÈGE

Inventor： BOSCHINI, Frédéric ,  BODART, Jérôme ,  CLOOTS, Rudi

IPC: B01J2/06 ,  B01J20/06 ,  B01J20/10 ,  B01J20/28 ,  B01J20/32 ,  C12N15/10 ,  C12Q1/6806 ,  B01J20/08 ,  B01J20/103 ,  B01J20/28009 ,  B01J20/28011 ,  B01J20/28057 ,  B01J20/3204 ,  B01J20/3236 ,  B01J20/3293 ,  C12N15/1013

Abstract: The invention relates to a process for preparing core-shell particles comprising the steps of (i) providing a dispersion of primary magnetic particles having a mean diameter lower than 200 nm in a solvent; (ii) adding one or more (semi-)metal (oxyhydr)oxide(s) and/or one or more precursor(s) of a (semi-)metal (oxyhydr)oxide to said dispersion; (iii) optionally adding a hydrolysis agent for said one or more precursor(s); (iv) injecting the dispersion in a spray dryer; whereby a (semi-)metal (oxyhydr)oxide shell is formed on the magnetic particles during spray drying. The invention also relates to particles obtainable by said process, to a formulation of said particles in a solvent and to the use of said particles or said formulation for RNA or DNA extraction.

公开(公告)号：WO2021030268A2

公开(公告)日：2021-02-18

申请号：PCT/US2020/045619

申请日：2020-08-10

Applicant: NUTCRACKER THERAPEUTICS, INC.

Inventor： ELDRIDGE, Benjamin ,  WEN, Ximiao

IPC: C12P19/34 ,  C12Q1/68 ,  A61P35/00 ,  B01L2400/0487 ,  B01L3/502707 ,  B01L3/502769 ,  C12M41/48 ,  C12N15/101 ,  C12N15/1013 ,  C12N15/1017 ,  G01N2021/0187 ,  G01N21/01

Abstract: Apparatuses and methods are described herein for processing polynucleotides in a sealed path environment. The apparatuses include optical sensors to monitor operations and to track material usage for good manufacturing practice.

公开(公告)号：WO2023012333A2

公开(公告)日：2023-02-09

申请号：PCT/EP2022/072082

申请日：2022-08-05

Applicant: NOVOCHIZOL SA

Inventor： FOMENKO, Vladislav ,  KARGAPOLOV, Yuriy ,  KHARCHENKO, Alexey ,  SERGEEVICHEV, David

IPC: A01N25/04 ,  A61L27/48 ,  A61L27/52 ,  A61L27/56 ,  B33Y70/00 ,  C08B37/08 ,  C08J5/22 ,  C08J9/28 ,  C08L29/04 ,  C09K17/20 ,  C12N5/00 ,  A61L2400/06 ,  A61L27/26 ,  A61L27/38 ,  B09C1/08 ,  C08B37/003 ,  C08J2205/02 ,  C08J2205/044 ,  C08J2207/10 ,  C08J2301/12 ,  C08J2329/04 ,  C08J2405/08 ,  C08J3/075 ,  C08J9/0061 ,  C08L1/12 ,  C08L5/08 ,  C09D105/08 ,  C12N15/1013 ,  C12N2533/72 ,  C12N2533/78 ,  C12N2537/10 ,  C12N5/0062 ,  C12N5/0068

Abstract: The present invention is directed to gels, cryogels and other polymer scaffolds, solid surfaces, aggregates, films and coatings comprising a soluble chemically modified cross-linked chitosan useful for 3D cell and organoid bioprinting and other uses in biology, medicine, bioanalytics and environmental sciences and methods of preparation thereof.

公开(公告)号：WO2021150903A1

公开(公告)日：2021-07-29

申请号：PCT/US2021/014631

申请日：2021-01-22

Applicant: ATRECA, INC.

Inventor： CARROLL, Sean ,  WITHEY, Gary ,  BOYARSKIY, Sergey

IPC: C12N15/10 ,  C12N15/1006 ,  C12N15/1013 ,  C12N15/1075 ,  C12N15/1096

Abstract: Provided are high throughput methods for physically linking cDNA molecules derived from mRNA molecules expressed by the same cell, and libraries of linked cDNA molecules produced by the methods. The methods comprise reverse transcribing mRNA from a single cell in a first container to produce cDNA molecules, and linking the cDNA molecules in a second container. The methods unexpectedly produced libraries of cDNA molecules with an increase in the number of molecules that are correctly linked to other molecules derived from the same cell.

公开(公告)号：WO2022236863A1

公开(公告)日：2022-11-17

申请号：PCT/CN2021/095043

申请日：2021-05-21

Applicant: 厦门依加成科技有限公司

Inventor： 徐方成

IPC: C12N7/02 ,  C07K1/14 ,  C12N1/02 ,  C12N5/071 ,  C12N15/10 ,  B01J20/10 ,  B01J20/26 ,  B01J20/28 ,  B01J20/30 ,  B03C1/00 ,  B01J20/103 ,  B01J20/261 ,  B01J20/28007 ,  B01J20/28009 ,  B01J20/28021 ,  B01J20/28047 ,  B01J20/28085 ,  C12N15/1013 ,  C12N2509/00 ,  C12N5/0602 ,  C12N7/00

Abstract: 一种表面具有功能化斑点的微球，包括微球主体和镶嵌在所述微球主体顶部的功能化斑点，所述微球主体的底部内嵌有重力球，所述重力球用于调整所述微球主体的重心，使所述功能化斑点保持向上；所述微球的制备方法和应用，具有功能化斑点的微球可用于生物活性颗粒的吸附和分离。

公开(公告)号：WO2021231781A1

公开(公告)日：2021-11-18

申请号：PCT/US2021/032321

申请日：2021-05-13

Applicant: QUIDEL CORPORATION

Inventor： KEHLE, Johannes ,  JACONO, Bruce ,  KROLL, Werner ,  SUR, Kunal ,  PARPIA, Zaheet ,  ELIOPOULOS, Andrew ,  MOROVICH, Matthew ,  MCDANIELS-DAVIDSON, Jesse ,  BJARNASON, Jon ,  WITKOWICKI, Jesse ,  LINHOFF, Todd ,  PACK, Todd Denison

IPC: B01L3/00 ,  G01N35/04 ,  B01L2200/021 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/0609 ,  B01L2300/0663 ,  B01L2300/0672 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/0683 ,  B01L3/502 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/5029 ,  B01L3/527 ,  B01L3/561 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/6848 ,  G01N21/55 ,  G01N35/0098

Abstract: A system for analysis of a sample to aid in diagnosis and/or detection of the presence or absence, and/or the identity of, an analyte in the sample is provided. A cartridge with reagents to isolate nucleic acid from a sample inserted in the cartridge and to amplify the isolated nucleic acid, and an instrument that interacts with the cartridge, together provide a self-contained sample to answer system for detection, identification, differentiation and/or quantification of a target nucleic acid in a sample.

公开(公告)号：WO2021259960A1

公开(公告)日：2021-12-30

申请号：PCT/EP2021/067049

申请日：2021-06-22

Applicant: GNA BIOSOLUTIONS GMBH

Inventor： STEHR, Joachim ,  BUERSGENS, Federico ,  REBUFFO-SCHEER, Cecilia ,  GROTZ, Bettina ,  DUCZEK, Juliane ,  SCHMIDBAUER, Simon

IPC: C12Q1/6806 ,  B01L7/00 ,  C12N15/10 ,  B01L2200/0668 ,  B01L2300/0851 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L7/52 ,  C12N15/1013

Abstract: Bereitgestellt wird ein Verfahren zur Extraktion einer Target-Nukleinsäure (12) aus einer Probenflüssigkeit (30). Das Verfahren umfasst ein Bereitstellen einer Probenflüssigkeit (30) mit der Target-Nukleinsäure (12) in einem Reaktionsgefäß (22) und ein Bereitstellen von magnetischen Mikropartikeln (10) in der Probenflüssigkeit (30), die jeweils mit zumindest einer Extraktionsnukleinsäure (16) funktionalisiert sind, wobei die Extraktionsnukleinsäuren (16) zumindest teilweise komplementär zur Target-Nukleinsäure (12) sind. Ferner umfasst das Verfahren ein Hybridisieren zumindest eines Teils der Target-Nukleinsäure (12) mit einer der Extraktionsnukleinsäuren (16) und Binden der Target-Nukleinsäure (12) über die Extraktionsnukleinsäure (16) an eines der magnetischen Mikropartikeln (10). Außerdem umfasst das Verfahren ein Bereitstellen eines Magnetfeldes in dem Reaktionsgefäß (22) derart, dass zumindest ein Teil der mit der Target-Nukleinsäure (12) verbundenen magnetischen Mikropartikeln (10) an einer Gefäßwand (22a, 22b, 22c) des Reaktionsgefäßes (22) sedimentiert. Ferner werden Vorrichtungen und Verfahren zur Amplifikation einer Target-Nukleinsäure (12), die Verwendung von magnetischen Mikropartikeln (10) zur Extraktion einer Target-Nukleinsäure (12) und ein magnetisches Mikropartikel bereitgestellt.

公开(公告)号：WO2021258735A1

公开(公告)日：2021-12-30

申请号：PCT/CN2021/074681

申请日：2021-02-01

Applicant: 广州东盛生物科技有限公司

Inventor： 刘远东 ,  孟薇 ,  陈玫颖 ,  黄勤 ,  陈晴晴 ,  周宁

IPC: C12N15/10 ,  C12N15/1013 ,  C12N7/00

Abstract: 一种病毒保存液，其包含以下成分：异丙醇或乙醇、盐酸胍、三（羟甲基）氨基甲烷盐酸盐、乙二胺四乙酸。

公开(公告)号：WO2021030271A2

公开(公告)日：2021-02-18

申请号：PCT/US2020/045625

申请日：2020-08-10

Applicant: NUTCRACKER THERAPEUTICS, INC.

Inventor： DEUTSCH, Samuel ,  ELDRIDGE, Benjamin ,  McKINLAY, Colin ,  WEN, Ximiao ,  NATH, Sangeeta ,  KHANDROS, Igor ,  EVANS, Robert

IPC: C12N15/10 ,  C12N15/1096 ,  B01J19/00 ,  C12P19/34 ,  A61P35/00 ,  B01L2400/0487 ,  B01L3/502707 ,  B01L3/502769 ,  C12M41/48 ,  C12N15/101 ,  C12N15/1013 ,  C12N15/1017 ,  G01N2021/0187 ,  G01N21/01

Abstract: Methods and apparatuses for making and using therapeutics, including in particular mRNA therapeutics, that separate double- stranded RNA from single- stranded RNA as part of a continuous flow. These methods and apparatuses may include formulation of an RNA therapeutic using a permeable insert integrated into a microfluidic path device. In particular, these methods and apparatuses may include formulation of an RNA therapeutic by removing dsRNA from a solution of RNA by within a microfluidic path device including a cellulose material.