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27 条记录 (耗时 0.007 秒)

    Purification of superoxide dismutase
    1.
    发明公开
    Purification of superoxide dismutase 失效
    超氧化物歧化酶的纯化

    公开(公告)号：EP0188053A3

    公开(公告)日：1988-04-27

    申请号：EP85305900

    申请日：1985-08-19

    申请人： Friesen, Albert D. ,  Weselake, Randall J.

    发明人： Friesen, Albert D.

    IPC分类号： C12N09/02

    CPC分类号： C12N9/0089

    摘要： A process for purifying the enzyme, superoxide dismutase, by passing an aqueous solution containing the enzyme, which is free from hemoglobin, through a metal chelating affinity chromatographic column, for example an immobilized copper ion column, and eluting an enzyme solution having increased specific activity.

    摘要翻译： 通过使含有不含血红蛋白的酶的水溶液通过金属螯合亲和层析柱例如固定的铜离子柱，通过使具有增加的比活性的酶溶液进行洗脱来纯化酶超氧化物歧化酶的方法 。

    Superoxide dismutase combined with a poly(alkylene oxide
    2.
    发明公开
    Superoxide dismutase combined with a poly(alkylene oxide 失效
    与聚（亚烷基氧化物）组合的超氧化物歧化酶

    公开(公告)号：EP0200467A3

    公开(公告)日：1988-02-24

    申请号：EP86303058

    申请日：1986-04-23

    申请人： AJINOMOTO CO., INC.

    发明人： Yabuki, Akira ,  Iwashita, Yuji

    IPC分类号： C12N09/02 ,  C12N09/96 ,  A61K37/50

    CPC分类号： C12N9/96 ,  A61K38/446 ,  A61K47/60 ,  C12N9/0089

    摘要： Superoxide dismutase is chemically modified with poly(alkylene oxide), the modified superoxide dismutase having a molecular structure in which both ends of a poly(alkylene oxide) molecule are attached to superoxide dismutase. The modified superoxide dismutase can be used to remove toxic substances derived from oxygen, from the blood circulation in a living body, and has a longer half life in the blood circulation as compared to superoxide dismutase which has not been modified.

    Bilirubin-specific enzymes and their use in analytical elements and methods
    3.
    发明公开
    Bilirubin-specific enzymes and their use in analytical elements and methods 失效
    双酚A特异性酶及其在分析元素和方法中的应用

    公开(公告)号：EP0140004A3

    公开(公告)日：1985-06-26

    申请号：EP84110208

    申请日：1984-08-28

    申请人： EASTMAN KODAK COMPANY (a New Jersey corporation)

    发明人： Wu, Tai-Wing

    IPC分类号： C12N09/02 ,  C12Q01/00

    CPC分类号： C12N9/0004 ,  C12Q1/34 ,  Y10S435/805 ,  Y10T436/146666

    摘要： A bilirubin-specific enzyme is obtained from various higher plant sources of the families Solanaceae, Musaceae and Liliaceae. Specific plant sources include eggplants, tomatoes and potatoes from the Solanaceae family, bananas from the Musaceae family, and onions and garlic from the Liliaceae family. The enzyme can be used in wet and dry analytical methods either in assays for bilirubin (unconjugated or conjugated forms or total bilirubin) or to suppress bilirubin interference in assays for other analytes. The enzyme can also be used in a bilirubin-degrading method for treating an aqueous liquid, e.g. whole blood, which contains excess bilirubin.

    Novel superoxide dismutase and process for preparation thereof
    6.
    发明公开
    Novel superoxide dismutase and process for preparation thereof 失效
    新型超氧化物歧化酶及其制备方法

    公开(公告)号：EP0218480A3

    公开(公告)日：1988-04-20

    申请号：EP86307659

    申请日：1986-10-03

    申请人： Suntory Limited

    发明人： Asami, Sumio ,  Amano, Norihide ,  Amachi, Teruo ,  Yoshizumi, Hajime

    IPC分类号： C12N09/02

    CPC分类号： C12N9/0089 ,  Y10S435/822

    摘要： A novel superoxide dismutase having the following properties: (a) action: the superoxide dismutase disproportionates two superoxide radicals to a hydrogen peroxide and a molecular oxygen; (b) substrate specificity: the superoxide dismutase acts on a superoxide radical: (c) optimum pH: the optimum a pH is about 8; (d) stable pH: the superoxide dismutase is stable at pH of 4.5 to 8.0 (when the superoxide dismutase is treated at 25°C for 60 minutes, the residual activity is at least 80%); (e) thermal stability: when the residual activity is measured after the heat treatment for 10 minutes at a pH of 7.8, the superoxide dismutase is stable at treatment temperatures of up to about 50°C, and the residual activity is 90% at a treatment temperature of 60°C, and 50% at a treatment temperature of 70°C; (f) molecular weight: the molecular weight is about 8.8 x 10⁴, as determined according to the gel filtration method; (g) absorption spectrum of ultraviolet and visible region: the maximum absorbance appears at about 280 nm, the minimum absorbance appears at about 250 nm and a shoulder appears at about 290 nm; (h) molecular extinction coefficient: the molecular extinction coefficient at 280 nm is about 1.45 x 10⁵ M⁻¹cm⁻¹; (i) inhibition: the activity is not inhibited by an addition of 1 mM potassium cyanide and exponential inactivation is caused with the lapse of time by an addition of 5 mM hydrogen peroxide; and (j) metal analysis: the superoxide dismutase contains 1.56 g-atoms of iron per mole of oxygen but does not contain copper and manganese; and a process for the preparation of the above-mentioned superoxide dismutase which comprises culturing a superoxide dismutase-producing strain belonging to the genus Norcardopsis in a culture medium to form and accumulate a superoxide dismutase in the culture medium, and recovering the superoxide dismutase from the cultured product.

    摘要翻译： 一种由Nocardiopsis属超氧化物歧化酶生产菌株制备的新型超氧化物歧化酶。 该酶是分子量约为8.8×10 4的四聚物，由约2.2×10 4的亚单位组成。 超氧化物歧化酶每摩尔含有1.56克原子铁，但不含铜或锰。 浓度为1mM的氰化钾不抑制活性，但是由5mM过氧化氢引起指数失活。