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    METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL
    92.
    发明公开
    METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL 有权
    用于生产角膜内皮

    公开(公告)号：EP2733201A4

    公开(公告)日：2015-01-21

    申请号：EP12815195

    申请日：2012-07-13

    申请人： UNIV OSAKA

    发明人： HAYASHI RYUHEI ,  HARA SUSUMU ,  KAGEYAMA TOMOFUMI ,  NISHIDA KOHJI

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2501/115 ,  C12N2501/48 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

    摘要： The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.

    METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS
    94.
    发明公开
    METHOD OF EFFICIENTLY ESTABLISHING INDUCED PLURIPOTENT STEM CELLS 审中-公开
    VERFAHREN ZUR EFFIZIENTEN HERSTELLUNG INDUZIERTER PLURIPOTENTER STAMMZELLEN

    公开(公告)号：EP2462225A4

    公开(公告)日：2013-12-18

    申请号：EP10806584

    申请日：2010-08-06

    申请人： UNIV KYOTO

    发明人： OKITA KEISUKE ,  NAKAGAWA MASATO ,  YAMANAKA SHINYA

    IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N15/85

    CPC分类号： C12N15/85 ,  C12N5/0696 ,  C12N2501/40 ,  C12N2501/48 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2502/13 ,  C12N2506/1361 ,  C12N2510/00 ,  C12N2800/108 ,  C12N2800/30

    摘要： Provided is a method of producing an iPS cell, comprising bringing (a) Oct3/4 or a nucleic acid that encodes the same, (b) Klf4 or a nucleic acid that encodes the same, and (c) Sox2 or a nucleic acid that encodes the same, as well as (d1) L-Myc or a nucleic acid that encodes the same and/or (d2) a functional inhibitor of p53, into contact with a somatic cell. It is preferable that (a) a nucleic acid that encodes Oct3/4, (b) a nucleic acid that encodes Klf4, (c) a nucleic acid that encodes Sox2, (d1) a nucleic acid that encodes L-Myc and (e) a nucleic acid that encodes Lin28 or Lin28b be inserted into an episomal vector having loxP sequences placed in the same orientation on the 5′ and 3′ sides of a vector constituent essential for the replication of the vector, that (d2) a nucleic acid that encodes an shRNA against p53 be inserted into a vector ensuring transient expression (plasmid vector and the like), and that all these nucleic acids be transferred to a somatic cell.

    摘要翻译： 本发明提供生产iPS细胞的方法，其包括使（a）Oct3 / 4或其编码核酸，（b）Klf4或编码其的核酸，和（c）Sox2或 （d1）L-Myc或编码它们的核酸和/或（d2）p53功能性抑制剂编码相同的基因，使其与体细胞接触。 （a）编码Oct3 / 4的核酸，（b）编码Klf4的核酸，（c）编码Sox2的核酸，（d1）编码L-Myc的核酸和（e） ）将编码Lin28或Lin28b的核酸插入具有在5'端以相同方向放置的loxP序列的游离型载体中， 和3' 将（d2）编码针对p53的shRNA的核酸插入到保证瞬时表达的载体（质粒载体等）中，并转移所有这些核酸 到体细胞。

    METHOD FOR PRODUCING ANTIGEN-SPECIFIC B CELL POPULATION
    100.
    发明公开
    METHOD FOR PRODUCING ANTIGEN-SPECIFIC B CELL POPULATION 有权
    VERFAHREN ZUR ERZEUGUNG抗生素B-ZELLEN-POPULATIONEN

    公开(公告)号：EP2495312A1

    公开(公告)日：2012-09-05

    申请号：EP10826677.6

    申请日：2010-10-25

    申请人： Tokyo University Of Science Educational Foundation Administrative Organization

    发明人： KITAMURA, Daisuke ,  NOJIMA, Takuya

    IPC分类号： C12N5/0781 ,  C12P21/08

    CPC分类号： C12N5/0635 ,  C12N2501/23 ,  C12N2501/25 ,  C12N2501/48 ,  C12N2501/52

    摘要： The present invention provides a method for producing an antigen-specific B cell population comprising IgG-positive B cells specific to a specific antigen, the method comprising: culturing IgG-positive B cells together with the specific antigen in the presence of IL-21, while conferring stimulation to the IgG-positive B cells via CD40, a BAFF receptor and Fas; and screening for antigen-specific B cells specific to the specific antigen to obtain an antigen-specific B cell population comprising the IgG-positive B cells specific to the specific antigen.

    摘要翻译： 本发明提供一种包含特异性抗原特异性的IgG阳性B细胞的抗原特异性B细胞群的方法，所述方法包括：在IL-21存在下，与特异性抗原一起培养IgG阳性B细胞， 同时通过CD40，BAFF受体和Fas赋予对IgG阳性B细胞的刺激; 筛选特异性抗原特异性的抗原特异性B细胞，得到包含特异性抗原特异性的IgG阳性B细胞的抗原特异性B细胞群。