公开(公告)号：EP1644491A2

公开(公告)日：2006-04-12

申请号：EP04753654.5

申请日：2004-05-28

申请人： CELL GENESYS, INC.

发明人： LI, Yuanhao ,  FARSON, Deborah ,  TAO, Luqun ,  YU, De Chao

IPC分类号： C12N5/10 ,  C12N15/10 ,  C12N15/33 ,  C12N15/34 ,  C12N15/63 ,  C12N15/64 ,  C12N15/861

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： Adenovirus packaging cell lines for growth of E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA), are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

公开(公告)号：EP1196566B1

公开(公告)日：2006-02-01

申请号：EP00945309.3

申请日：2000-07-11

申请人： GENENTECH, INC.

发明人： CHISHOLM, Vanessa ,  CROWLEY, Craig, W. ,  KRUMMEN, Lynne, A. ,  MENG, Yu-Ju, G.

IPC分类号： C12N15/12 ,  C12N15/10 ,  C12N15/62 ,  C12N15/65 ,  C12N15/69 ,  C12N15/85 ,  C07K14/435 ,  C07K14/71 ,  C07K16/42 ,  C12Q1/68

CPC分类号： C12N15/85 ,  C07K14/43595 ,  C07K14/71 ,  C07K16/4291 ,  C07K2319/00 ,  C12N15/65 ,  C12N15/69 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44

摘要： Vectors and methods for efficient isolation of recombinant cells expressing high levels of a desired protein are provided. The vectors comprise an amplifiable selectable gene, a fluorescent protein gene, and a gene encoding a desired product in a manner that optimizes transcriptional and translational linkage.

公开(公告)号：EP1425400A4

公开(公告)日：2005-10-12

申请号：EP02773370

申请日：2002-09-12

申请人： CALIFORNIA INST OF TECHN

发明人： BALTIMORE DAVID ,  HONG ELIZABETH J ,  LOIS-CABALLE CARLOS ,  PEASE SHIRLEY

IPC分类号： C12N15/09 ,  A01K67/027 ,  A61K31/7088 ,  A61K38/00 ,  A61K48/00 ,  A61P31/18 ,  C12N15/113 ,  C12N15/85 ,  C12N15/867 ,  C12N15/63 ,  A01K67/00 ,  C12N15/00

CPC分类号： C12N15/86 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2227/40 ,  A61K38/00 ,  A61K48/00 ,  C12N15/1132 ,  C12N15/1138 ,  C12N15/8509 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/30 ,  C12N2740/16043 ,  C12N2800/60 ,  C12N2830/003 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.

公开(公告)号：EP1317560A4

公开(公告)日：2005-10-05

申请号：EP01968599

申请日：2001-09-07

申请人： GEN HOSPITAL CORP

发明人： SEED BRIAN ,  WRIGHT FREEMAN MASON ,  KOVTUN ALEXANDER ,  MURAKAWA MASAHIRO ,  PARK EUN-CHUNG ,  WANG XINZHONG

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K48/00 ,  A61P31/22 ,  A61P35/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/63 ,  C12N15/861 ,  C12N15/86 ,  C12N15/11 ,  C12N15/64 ,  C12N15/65

CPC分类号： C12N15/86 ,  A61K48/00 ,  C12N15/63 ,  C12N15/635 ,  C12N2710/10343 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/205 ,  C12N2830/38 ,  C12N2830/85 ,  C12N2840/203 ,  C12N2840/44

公开(公告)号：EP1017837B1

公开(公告)日：2005-07-06

申请号：EP98944085.4

申请日：1998-09-23

申请人： Oxford Biomedica (UK) Limited

发明人： BEBBINGTON, Chris ,  KINGSMAN, Susan ,  UDEN, Mark ,  KINGSMAN, Alan ,  MITROPHANOS, Kyriacos

IPC分类号： C12N15/86 ,  C12N7/01 ,  C12N5/10 ,  C12N15/63 ,  C12N15/53 ,  A61K48/00

CPC分类号： C12N15/63 ,  A61K48/00 ,  C07K14/005 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2740/13043 ,  C12N2740/15022 ,  C12N2740/15043 ,  C12N2760/20122 ,  C12N2810/40 ,  C12N2810/60 ,  C12N2810/6054 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/48 ,  C12N2830/50 ,  C12N2840/203 ,  C12N2840/44

摘要： A retroviral vector is described. The retroviral vector comprises a functional splice donor site and a functional splice acceptor site; wherein the functional splice donor site and the functional splice acceptor site flank a first nucleotide sequence of interest ('NOI'); wherein the functional splice donor site is upstream of the functional splice acceptor site; wherein the retroviral vector is derived from a retroviral pro-vector; wherein the retroviral pro-vector comprises a first nucleotide sequence ('NS') capable of yielding the functional splice donor site and a second NS capable of yielding the functional splice acceptor site; wherein the first NS is downstream of the second NS; such that the retroviral vector is formed as a result of reverse transcription of the retroviral pro-vector.

公开(公告)号：EP1548118A2

公开(公告)日：2005-06-29

申请号：EP05005691.0

申请日：1995-06-07

申请人： GENVEC, INC.

发明人： Kovesdi, Imre ,  Brough, Douglas E. ,  McVey, Duncan L. ,  Bruder, Joseph T. ,  Lizonova, Alena

IPC分类号： C12N15/861 ,  C07K14/075 ,  C12N15/34 ,  A61K48/00 ,  C07K14/47 ,  C12N5/10 ,  C12N7/01 ,  C12N15/12

CPC分类号： C12N7/00 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/5256 ,  C07K14/005 ,  C07K14/4712 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2810/60 ,  C12N2810/6081 ,  C12N2830/002 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44 ,  Y10S977/799

摘要： The present invention provides multiply deficient adenoviral vectors and complementing cell lines. Also provided are recombinants of the multiply deficient adenoviral vectors and a therapeutic method, particularly relating to gene therapy, vaccination, and the like, involving the use of such recombinants.

摘要翻译： 本发明提供多重缺陷型腺病毒载体和补体细胞系。 还提供了多重缺陷型腺病毒载体的重组体和涉及使用这种重组体的治疗方法，特别是与基因治疗，疫苗接种等相关的治疗方法。

公开(公告)号：EP0981637B1

公开(公告)日：2005-05-25

申请号：EP98910109.2

申请日：1998-03-09

申请人： Biogen Idec Inc.

发明人： REFF, Mitchell, E. ,  BARNETT, Richard, Spence ,  McLACHLAN, Karen, Retta

IPC分类号： C12N15/90 ,  C12N15/85 ,  C12Q1/68 ,  C12N5/10 ,  C12N9/12 ,  C12N15/13 ,  C07K16/28 ,  C12N15/12 ,  C07K14/705 ,  G01N33/53 ,  C12N15/62 ,  C07K19/00

CPC分类号： C07K16/2851 ,  C07K14/70521 ,  C07K16/2896 ,  C07K2319/02 ,  C12N15/85 ,  C12N15/907 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/20 ,  C12N2840/44

摘要： A method for achieving site specific integration of a desired DNA at a target site in a mammalian cell via homologous recombination is described. This method provides for the reproducible selection of cell lines wherein a desired DNA is integrated at a predetermined transcriptionally active site previously marked with a marker plasmid. The method is particularly suitable for the production of mammalian cell lines which secrete mammalian proteins at high levels, in particular immunoglobulins. Vectors and vector combinations for use in the subject cloning method are also provided.

公开(公告)号：EP0857217B1

公开(公告)日：2005-04-13

申请号：EP96936537.8

申请日：1996-10-16

申请人： DANA-FARBER CANCER INSTITUTE

发明人： MARASCO, Wayne A. ,  RICHARDSON, Jennifer ,  PAROLIN, Maria Cristina ,  SODROSKI, Joseph G.

IPC分类号： C12N15/86

CPC分类号： C07K14/005 ,  C12N15/86 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/16043 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention is related to vectors and methods for increasing the expression of a desired gene product. Preferably this invention is used with genes expressing proteins that are not well tolerated by mammalian cells or where high levels of expression are necessary. In certain preferred embodiments it can be used as part of a multi-tiered expression system and with methods of intracellularly targeting a molecule.

公开(公告)号：EP1507865A2

公开(公告)日：2005-02-23

申请号：EP03732512.3

申请日：2003-05-30

申请人： MAX-DELBRÜCK-CENTRUM FÜR MOLEKULARE MEDIZIN ,  Netherland Cancer Institute

发明人： MISKEY, Csaba ,  IZSVAK, Zsuzsanna ,  IVICS, Zoltan ,  PLASTERK, Ronald

IPC分类号： C12N15/90 ,  C12N9/22 ,  C12N15/85 ,  A61K38/00 ,  C12N5/06 ,  A01K67/027

CPC分类号： C12N15/90 ,  C12N15/85 ,  C12N2800/60 ,  C12N2800/90 ,  C12N2840/44

摘要： The present invention relates to a transposon-based DNA integration system comprising (a) a transposon which is devoid of a polynucleotide encoding a functional transposase and which comprises a polynucleotide of interest, wherein the transposon comprises inverted repeats having a degree of identity with the repeats within SEQ ID NO: 2 and its inverted repeat, respectively, of at least 90%; and (b) a transposase having at its N-terminus a DNA binding domain comprising the sequences of SEQ ID NO: 3 and 4; or (c) a polynucleotide encoding the transposase of (b). The present invention further relates to a method of transferring a polynucleotide of interest into cells of a vertebrate comprising the step of introducing the transposon-based DNA integration system of the invention into said cells. In addition, the invention relates to a method of effecting RNAi comprising (a) stably introducing a transposon comprising an expression cassette expressing a short interfering RNA and a selectable marker gene as part of the trans poson-based DNA integration system of the invention into a cell; (b) selecting for cells expressing the selectable marker; and (c) assessing whether the transcription/translation of the desired gene is effected by RNAi. A further embodiment of the invention is a method of gene trapping genes comprising the steps of (a) introducing the transposon-based DNA integration system of the invention into a cell; and (b) assessing for the expression of a selectable marker wherein expression of a selectable marker is indicative of integration of the transposon into a transcibed gene of the cell.

公开(公告)号：EP1414948A2

公开(公告)日：2004-05-06

申请号：EP02742400.1

申请日：2002-07-10

申请人： Idec Pharmaceuticals Corporation

发明人： REFF, Mitchell ,  BETENBAUGH, Michael, J. ,  FIGUEROA, Bruno, Jr. ,  AILOR, Eric ,  HARDWICK, Marie

IPC分类号： C12N5/10 ,  C12N5/06 ,  C12N5/08 ,  C12P21/00 ,  C07H21/00 ,  C07K14/00

CPC分类号： C07K14/4747 ,  A61K39/00 ,  A61K48/005 ,  C07K14/721 ,  C07K16/2875 ,  C07K2319/00 ,  C12N15/67 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/002 ,  C12N2830/15 ,  C12N2840/20 ,  C12N2840/44

摘要： The present invention relates to preventing or delaying programmed cell death by expressing one or more anti-apoptotic polypeptides in a cell such that programmed cell death in the cell is prevented or delayed. The present invention also relates to increasing production of a cell-related product by expressing one or more anti-apoptotic polypeptides in a cell such that production of the cell-related product by the cell is increased. Recombinant cells useful for producing cell-related product or cellular therapy are also provided.