公开(公告)号：EP2343362B8

公开(公告)日：2016-05-18

申请号：EP10183677.3

申请日：2007-07-04

申请人： DPx Holdings B.V.

发明人： Zijlstra, Gerben Meile ,  Hof, Robert Patrick ,  Schilder, Jacob

IPC分类号： C12M3/06 ,  C12M1/12 ,  C12N5/07 ,  C12N5/079 ,  C12P21/00

CPC分类号： C12P21/00 ,  B01D15/26 ,  B01D69/08 ,  C07K1/18 ,  C07K1/22 ,  C07K1/34 ,  C07K16/00 ,  C12M23/28 ,  C12M29/04 ,  C12M29/18 ,  C12M47/10 ,  C12N5/0603 ,  C12N5/0621 ,  C12N2500/32 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2510/04 ,  C12P21/005

公开(公告)号：EP2925856A2

公开(公告)日：2015-10-07

申请号：EP13844534.1

申请日：2013-12-03

申请人： Biolamina AB

发明人： HOVATTA, Outi ,  TRYGGVASON, Karl

IPC分类号： C12N5/00 ,  C12N5/079

CPC分类号： C12N5/0621 ,  A61K9/0019 ,  A61K9/0048 ,  A61K35/30 ,  C12N5/0056 ,  C12N2500/92 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2506/02 ,  C12N2533/50 ,  C12N2533/52

摘要： The present disclosure relates to the use of laminin-521 in obtaining retinal pigment epithelium (RPE) cells. Pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in totally defined and xeno-free conditions. A first cell culture medium contains a growth factor, and a second cell culture medium does not contain growth factor. The stem cells are first exposed to the first cell culture medium, then exposed to the second cell culture medium for a longer time period. After a number of weeks, clinical grade RPE cells are obtained from the stem cells.

公开(公告)号：EP2401361A4

公开(公告)日：2013-09-04

申请号：EP10765022

申请日：2010-04-13

申请人： UNIV CALIFORNIA

发明人： SATO NOBORU

IPC分类号： C12N5/02 ,  C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2501/727

公开(公告)号：EP2499236A4

公开(公告)日：2013-05-22

申请号：EP10829622

申请日：2010-11-11

申请人： TECHNION RES & DEV FOUNDATION

发明人： AMIT MICHAL ,  ITSKOVITZ-ELDOR JOSEPH

IPC分类号： C12N5/02 ,  C12N5/071 ,  C12N5/0735

CPC分类号： C12N5/0602 ,  C12N5/0606 ,  C12N5/0696 ,  C12N2500/36 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/98 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/23 ,  C12N2506/45

摘要： Provided are serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3, ascorbic acid, xeno-free serum replacement and a lipid mixture. The media may also comprise an IL6R/IL6 chimera, or leukemia inhibitory factor (LIF); wherein the culture medium is capable of maintaining pluripotent stem cells in an undifferentiated state in the absence of feeder cell support. Also provided are cell cultures comprising pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using the same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems. Methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation are also provided.

摘要翻译： 提供了包含碱性成纤维细胞生长因子（bFGF），转化生长因子β-3，抗坏血酸，不含异种血清的血清替代物和脂质混合物的无血清培养基。 培养基还可以包含IL6R / IL6嵌合体或白血病抑制因子（LIF）; 其中所述培养基能够在不存在饲养细胞支持的情况下将多能干细胞保持在未分化状态。 还提供了包含多能干细胞如人胚胎干细胞和诱导的多能干细胞（iPS）细胞的细胞培养物，以及使用该多能干细胞用于使用二维或三维培养系统在未分化状态下扩增多能干细胞的方法。 还提供了在没有底物粘附和细胞包封的悬浮培养物中扩增iPS细胞的方法。

公开(公告)号：EP2529006A2

公开(公告)日：2012-12-05

申请号：EP11735298.9

申请日：2011-01-24

申请人： Ventria Bioscience

发明人： BARNETT, Michael ,  CROUGHAN, Matthew, S.

IPC分类号： C12N5/02 ,  C07K14/76 ,  C12N5/07 ,  C12P21/00

CPC分类号： C12N5/0018 ,  C07K14/765 ,  C12N15/8257 ,  C12N2500/76 ,  C12N2500/84 ,  C12N2500/92 ,  C12N2501/998

摘要： The invention relates to compositions, and uses thereof, which are beneficial for eukaryotic cells in culture, and methods for their use in promoting cell growth, viability and recombinant protein expression. The methods disclosed in the present application are useful, for example, for improving cell viability and in accelerating the rate of cell growth of cells grown in culture. In one aspect, the supplements of the invention are useful for improving or enhancing the yield of the recombinant proteins from the cell cultures.

公开(公告)号：EP2213724B1

公开(公告)日：2012-09-26

申请号：EP10004392.6

申请日：2005-10-12

申请人： Baxter International Inc. ,  Baxter Healthcare S.A.

发明人： Grillberger, Leopold ,  Reiter, Manfred ,  Mundt, Wolfgang ,  Dorner, Friedrich

IPC分类号： C12N5/02 ,  C12N7/02 ,  C12N1/16 ,  C12N1/20

CPC分类号： C12N5/0043 ,  C12N5/0682 ,  C12N2500/46 ,  C12N2500/74 ,  C12N2500/76 ,  C12N2500/92

摘要： The present invention relates to an animal protein-free cell culture medium, comprising at least one polyamine and at least one protein hydrolysate derived from the group consisting of plants and yeast, wherein the polyamine is present in the culture medium in a concentration ranging from 0.5 to 10 mg/L and the protein hydrolysate is present in a concentration ranging from 0.05 % (w/v) to about 1 % (w/v).

公开(公告)号：EP2390263A1

公开(公告)日：2011-11-30

申请号：EP11161155.4

申请日：2005-08-26

申请人： ARES TRADING S.A.

发明人： Fischer, Dina ,  Bernard, Alain ,  Ducommun, Paul ,  Rossi, Mara

IPC分类号： C07K14/565 ,  C12N5/00 ,  C07K1/18 ,  C07K1/20 ,  C07K1/22

CPC分类号： C12N5/0031 ,  A61K38/215 ,  C07K14/565 ,  C12N5/0037 ,  C12N5/06 ,  C12N15/00 ,  C12N2500/12 ,  C12N2500/32 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2510/02

摘要： The present invention relates to a process for the production of interferon beta, and to an interferon beta composition having a unique glycosylation pattern.

摘要翻译： 本发明涉及生产干扰素β的方法，以及具有独特糖基化模式的干扰素β组合物。

公开(公告)号：EP2250254A4

公开(公告)日：2011-09-07

申请号：EP09707613

申请日：2009-02-05

申请人： REGENERTECH PTY LTD

发明人： BHASIN VISHAL

IPC分类号： C12N5/071 ,  C12N5/074 ,  C12N5/077 ,  C12N5/078 ,  C12N5/08 ,  C12N5/06

CPC分类号： C12N5/0607 ,  A61K35/12 ,  C12N5/0634 ,  C12N5/0653 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0676 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/95 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/125 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/25 ,  C12N2501/385 ,  C12N2501/405 ,  C12N2501/41 ,  C12N2501/70 ,  C12N2506/1307 ,  C12N2506/1392

摘要： The present invention provides a method of producing progenitor cells, such as cells capable of being differentiated into a plurality of different cell types, from differentiated cells. Methods of using progenitor cells in differentiation and/or tissue or organ repair and/or regeneration and/or building are also provides. Methods of using progenitor cells in treatment and prophylaxis of conditions alleviated by administering stem cells or tissue or organ derived from stem cells to a subject or by grafting stem cells or tissue or organ derived from stem cells into a subject or by transplanting stem cells or tissue or organ derived from stem cells into a subject are also provided. Also included are progenitor cells and differentiated cells and/or tissues and/or organs derived therefrom, and kits comprising same.

公开(公告)号：EP2041259A1

公开(公告)日：2009-04-01

申请号：EP07765049.7

申请日：2007-07-04

申请人： DSM IP Assets B.V.

发明人： ZIJLSTRA, Gerben, Meile ,  HOF, Robert, Patrick ,  SCHILDER, Jacob

IPC分类号： C12M1/12 ,  C12M3/06 ,  C12N5/06

CPC分类号： C12P21/00 ,  B01D15/26 ,  B01D69/08 ,  C07K1/18 ,  C07K1/22 ,  C07K1/34 ,  C07K16/00 ,  C12M23/28 ,  C12M29/04 ,  C12M29/18 ,  C12M47/10 ,  C12N5/0603 ,  C12N5/0621 ,  C12N2500/32 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/99 ,  C12N2510/04 ,  C12P21/005

摘要： The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.

公开(公告)号：EP1992687A2

公开(公告)日：2008-11-19

申请号：EP08163979.1

申请日：2003-03-07

申请人： Vivalis

发明人： Pain, Bertrand ,  Guehenneux, Fabienne

IPC分类号： C12N5/06 ,  C12N5/10

CPC分类号： C12N5/0606 ,  C12N2500/90 ,  C12N2500/92 ,  C12N2500/95 ,  C12N2510/02

摘要： La présente invention se rapporte à un procédé d'obtention de lignées cellulaires aviaires, comprenant un sevrage progressif ou total des facteurs croissance, du sérum et/ou du tapis nourricier de sorte que les lignées établies sont des cellules adhérentes ou non-adhérentes capables de proliférer indéfiniment dans un milieu de culture de base. L'invention porte aussi sur les cellules dérivant de telles lignées qui sont particulièrement utiles pour la production de substances d'intérêt.

摘要翻译： 在没有生长因子，血清和/或饲养细胞的基础培养基中能够增殖的禽类细胞系（A）的制备是新的。 通过以下方式制备禽细胞系（A）：（a）在含有生长所需的所有因子和灭活饲养层的培养基中培养禽细胞; （b）通过改变培养基进行传代以从生长因子，血清和/或饲养层产生进行性或完整的断奶; 和（c）建立在不存在外源性生长因子，血清和/或饲养层的情况下可在基础培养基中增殖的（非）贴壁细胞系。 还包括通过新方法生产的细胞系的独立权利要求，该方法可以在（a）缺乏外源性生长因子的培养基中增殖至少50天，特别是600天; （b）具有降低的或零的血清含量和/或（c）缺乏饲养层。