公开(公告)号：EP2432884B1

公开(公告)日：2016-08-03

申请号：EP10720510.6

申请日：2010-05-24

申请人： Merial, Inc.

发明人： AUDONNET, Jean-christophe ,  JOLIVET, Edmond

IPC分类号： C12N15/73 ,  C12N15/63 ,  C12N15/68

CPC分类号： C12N15/73 ,  C12N15/635 ,  C12N15/85 ,  C12N2800/107 ,  C12N2800/24 ,  C12P21/00

公开(公告)号：EP2938363A1

公开(公告)日：2015-11-04

申请号：EP13815768.0

申请日：2013-12-24

申请人： Glycovaxyn AG ,  Eidgenössische Materialprüfungs- und Forschungsanstalt

发明人： IHSSEN, Julian ,  KOWARIK, Michael ,  THÖNY-MEYER, Linda Christiane

IPC分类号： A61K47/48 ,  C07K14/34 ,  C12N15/73

CPC分类号： C07K14/34

摘要： The present invention provides novel methods of producing diphtheria toxin. In particular, the present invention provides novel methods of producing nontoxic forms of diphtheria toxin, e.g., CRM197. The present invention also provides novel compositions comprising diphtheria toxin or nontoxic forms of diphtheria toxin, e.g., CRM197.

摘要翻译： 本发明提供了产生白喉毒素的新方法。 特别地，本发明提供了产生无毒形式的白喉毒素的新方法，例如CRM197。 本发明还提供了包含白喉毒素或无毒形式的白喉毒素（例如CRM197）的新型组合物。

公开(公告)号：EP2152748B1

公开(公告)日：2013-08-21

申请号：EP08768087.2

申请日：2008-06-03

申请人： Genentech, Inc.

发明人： SIEBEL, Christian, W. ,  WU, Yan

IPC分类号： C07K16/28 ,  A61K39/395 ,  A61P37/00 ,  A61K35/00 ,  A61P21/00 ,  A61P43/00 ,  C12N15/13 ,  A61P25/28 ,  C12N15/73 ,  C12N1/21

CPC分类号： C07K16/30 ,  A61K39/3955 ,  A61K45/06 ,  A61K2039/505 ,  C07K14/705 ,  C07K14/71 ,  C07K16/18 ,  C07K16/28 ,  C07K16/2863 ,  C07K16/3015 ,  C07K2317/14 ,  C07K2317/33 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92 ,  C12N5/10 ,  C12N5/12 ,  C12N15/09 ,  C12N15/63 ,  C12N2330/50

公开(公告)号：EP1754787B1

公开(公告)日：2012-01-25

申请号：EP06018386.0

申请日：1991-07-10

申请人： MEDIMMUNE LIMITED ,  Medical Research Council

发明人： McCafferty, John ,  Pope, Anthony Richard ,  Johnson, Kevin Stuart ,  Hoogenboom, Hendricus Renerus Jacobus Mattheus ,  Griffiths, Andrew David ,  Jackson, Ronald Henry ,  Holliger, Kaspar Philipp ,  Marks, James David ,  Clackson, Timothy Piers ,  Chiswell, David John ,  Winter, Gregory Paul ,  Bonnert, Timothy Peter

IPC分类号： C12N15/10 ,  C07K16/00 ,  C12N15/62 ,  C12N7/00 ,  C12N15/73 ,  C12N1/21 ,  C12R1/19

CPC分类号： C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

公开(公告)号：EP1695985B1

公开(公告)日：2011-03-09

申请号：EP05077948.7

申请日：1998-04-03

申请人： Genentech, Inc.

发明人： Wells, James A. ,  Baca, Manuel ,  Presta, Leonard G.

IPC分类号： C07K16/00 ,  C12N15/13 ,  C12N15/73 ,  A61K39/395

CPC分类号： C07K16/22 ,  A61K38/00 ,  C07K16/005 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/92

摘要： Described herein is a humanized antibody to vascular endothelial growth factor (VEGF). Also described herein is a method for rapidly producing and identifying framework mutations which improve the binding of humanized antibodies to their congnate antigens. In a preferred embodiment, non-human CDRs are grafted onto a human V 1 K I-V H III framework. Random mutagenesis of a small set of critical framework residues is also performed followed by monovalent display of the resultant library of antibody molecules on the surface of filamentous phage. The optimal framework sequences are then identified by affinity-based selection. Optionally, the selected antibodies can be further mutated so as to replace vernier residues which sit at the V L -V H interface by residues which match the non-human parent antibody. The methods described herein can be applied to any non-human antibody. Accordingly, humanized antibodies are provided.

公开(公告)号：EP0935664B1

公开(公告)日：2009-06-24

申请号：EP97944777.8

申请日：1997-08-21

申请人： EVAX Technologies AG

发明人： LUBITZ, Werner ,  JECHLINGER, Wolfgang ,  SZOSTAK, Michael ,  WITTE,Angela

IPC分类号： C12N15/73 ,  C12N15/10 ,  A61K39/02

CPC分类号： C12N15/73

摘要： The present invention concerns a process for selecting new PR- or PL-operator sequences of lambdoid phages which, compared to wild-type sequences, have a different thermostability for the binding of a repressor. In addition, the invention discloses new mutated PR- or PL- operator sequences and their use for temperature-regulated expression of genes and for producing improved vaccines.

公开(公告)号：EP1847605A3

公开(公告)日：2007-10-31

申请号：EP07006223.7

申请日：1991-07-10

申请人： CAMBRIDGE ANTIBODY TECHNOLOGY LIMITED ,  Medical Research Council

发明人： McCafferty, John ,  Pope, Anthony Richard ,  Johnson, Kevin Stuart ,  Hoogenboom, Hendricus Renerus Jacobus Mattheus ,  Griffiths, Andrew David ,  Jackson, Ronald Henry ,  Holliger, Kaspar Philipp ,  Marks, James David ,  Clackson, Timothy Piers ,  Chiswell, David John ,  Winter, Gregory Paul ,  Bonnert, Timothy Peter

IPC分类号： C12N15/10 ,  C07K16/00 ,  C12N15/62 ,  C12N7/00 ,  C12N15/73

CPC分类号： C07K16/005 ,  B01J2219/00718 ,  C07K16/00 ,  C07K16/18 ,  C07K16/26 ,  C07K16/34 ,  C07K16/40 ,  C07K16/44 ,  C07K16/462 ,  C07K16/468 ,  C07K19/00 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/34 ,  C07K2317/55 ,  C07K2317/62 ,  C07K2317/622 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/41 ,  C07K2319/735 ,  C12N15/1037 ,  C12N15/62 ,  C12N15/73 ,  C40B40/02 ,  G01N33/53 ,  G01N33/6857 ,  Y10S530/867

摘要： A member of a specific binding pair (sbp) is identified by expressing DNA encoding a genetically diverse population of such sbp members in recombinant host cells in which the sbp members are displayed in functional form at the surface of a secreted recombinant genetic display package (rgdp) containing DNA encoding the sbp member or a polypeptide component thereof, by virtue of the sbp member or a polypeptide component thereof being expressed as a fusion with a capsid component of the rgdp. The displayed sbps may be selected by affinity with a complementary sbp member, and the DNA recovered from selected rgdps for expression of the selected sbp members. Antibody sbp members may be thus obtained, with the different chains thereof expressed, one fused to the capsid component and the other in free form for association with the fusion partner polypeptide. A phagemid may be used as an expression vector, with said capsid fusion helping to package the phagemid DNA. Using this method libraries of DNA encoding respective chains of such multimeric sbp members may be combined, thereby obtaining a much greater genetic diversity in the sbp members than could easily be obtained by conventional methods.

摘要翻译： 特定结合对（sbp）的成员通过在重组宿主细胞中表达编码这种sbp成员的基因多样性群体的DNA来鉴定，其中sbp成员以功能形式显示在分泌的重组遗传展示包（rgdp ）含有编码sbp成员的DNA或其多肽组分，因为sbp成员或其多肽组分与rgdp的衣壳组分表达融合。 显示的sbps可以通过与互补sbp成员的亲和力来选择，并且从选择的rgdps恢复的DNA用于表达选定的sbp成员。 因此可以获得抗体sbp成员，其不同的链表达，一个融合至衣壳组分，另一个以游离形式与融合伴侣多肽结合。 噬菌粒可用作表达载体，所述衣壳融合有助于包装噬菌粒DNA。 使用这种方法，可以将编码这种多聚体sbp成员各自链的DNA文库组合起来，从而在sbp成员中获得比通过常规方法容易获得的更大的遗传多样性。

公开(公告)号：EP1773886A1

公开(公告)日：2007-04-18

申请号：EP05788681.4

申请日：2005-08-03

申请人： Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts

发明人： HOHEISEL, Jörg

IPC分类号： C07K16/30 ,  G01N33/53 ,  C12N15/73

CPC分类号： C07K16/30 ,  C07K16/005

摘要： Described is an improved method of selecting a member of a specific binding pair (sbp) having a desired specify, preferably an antibody, from a library expressing said member of a sbp, preferably a phage-display antibody library.

公开(公告)号：EP1609867A1

公开(公告)日：2005-12-28

申请号：EP05000339.1

申请日：2005-01-10

申请人： Feng Chia University ,  Taichung District Agriculture Research and Extension Station, Council of Agriculture

发明人： Chao, Yun-Peng ,  Wang, Zei-Wen ,  Chen, Po-Ting ,  Chen, Yu-Hsin

IPC分类号： C12N15/63 ,  C12N15/67 ,  C12N15/71 ,  C12N15/72 ,  C12N15/73 ,  C12N5/10

CPC分类号： C12N9/0036 ,  C07K14/805 ,  C07K2319/00 ,  C12N15/67 ,  C12N15/70

摘要： Disclosed herein are nucleic acid constructs and expression vectors for enhancing the production of recombinant polypeptides/proteins, and methods for the massive production of recombinant polypeptides/proteins, in which a first nucleic acid sequence encoding thioredoxin and a second nucleic acid sequence encoding hemoglobin are cloned into a host cell, thereby enhancing the capability of the thus formed recombinant host cell in producing a selected gene product, and thereby assisting said recombinant host cell in relieving intracellular stress due to the overproduction of said gene product.

摘要翻译： 公开在用于大规模生产重组多肽/蛋白质，其中第一核酸序列编码硫氧还蛋白和第二核酸序列编码的血红蛋白被克隆的核酸构建体和用于增强生产重组多肽/蛋白质的表达载体，和方法 入宿主细胞中，从而提高了由此形成的重组宿主细胞的能力在制造将所选基因产物，从而辅助所述重组宿主细胞在胞内释放应力由于所述基因产物的生产过剩。

公开(公告)号：EP1180153B1

公开(公告)日：2005-04-13

申请号：EP00932819.6

申请日：2000-05-24

申请人： Phage Biotechnology Corporation

发明人： KORDYUM, Vitaliy, A. ,  CHERNYKH, Svetlana, I. ,  SLAVCHENKO, Irina, Y. ,  VOZIANOV, Oleksandr, F.

IPC分类号： C12N15/73 ,  C07K14/60 ,  C12P21/02 ,  C12N7/00 ,  C12N1/06

CPC分类号： C12N7/00 ,  C07K14/60 ,  C12N1/06 ,  C12N15/73 ,  C12N2795/10143 ,  C12P21/02

摘要： This invention relates to a method for enhancing the production of soluble and biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage μ with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.