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    METHOD FOR PREPARING METAL NANOPARTICLE USING METAL BINDING PROTEIN
    31.
    发明公开
    METHOD FOR PREPARING METAL NANOPARTICLE USING METAL BINDING PROTEIN 审中-公开
    一种用于生产金属纳米粒子使用金属结合蛋白

    公开(公告)号：EP2021480A1

    公开(公告)日：2009-02-11

    申请号：EP07746028.5

    申请日：2007-04-17

    Applicant: Korea Advanced Institute of Science and Technology

    Inventor： LEE, Sang Yup ,  PARK, Tae Jung

    IPC: C12N15/70 ,  C12N9/02

    CPC classification number: C07K14/825 ,  B82Y5/00 ,  B82Y15/00 ,  C12N9/104 ,  C12P3/00 ,  G01N33/531 ,  G01N33/588 ,  Y10T428/2982

    Abstract: The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores.

    MUTANT MICROORGANISM HAVING A HYDROCARBON-GENERATING ABILITY AND METHOD FOR PRODUCING HYDROCARBON USING SAME
    38.
    发明公开
    MUTANT MICROORGANISM HAVING A HYDROCARBON-GENERATING ABILITY AND METHOD FOR PRODUCING HYDROCARBON USING SAME 审中-公开
    以能力生烃和方法的油气生产订单微生物突变

    公开(公告)号：EP2592138A2

    公开(公告)日：2013-05-15

    申请号：EP11792710.3

    申请日：2011-06-10

    Applicant: Korea Advanced Institute Of Science And Technology

    Inventor： LEE, Sang Yup ,  CHOI, Yong Jun

    IPC: C12N1/21 ,  C12N15/52 ,  C12P5/00

    CPC classification number: C12P5/00 ,  C07K14/245 ,  C12N9/001 ,  C12N9/18 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70 ,  C12P5/005 ,  C12P5/02 ,  C12P5/026

    Abstract: The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane.

    Abstract translation: 本发明涉及到的微生物的变体具有生产烃类，包括烷烃的能力，并且生产烃，包括烷烃，使用相同的，更具体的方法中，通过引入基因编码的酶转换脂肪酸的得到的微生物的变体 酰基-ACP向游离脂肪酸，编码酶的游离脂肪酸转化成脂肪酰辅酶A，编码酶转化脂肪酰辅酶A的一个基因，以脂肪醛和编码酶的基因转化脂肪醛为烷烃到微生物的基因 改进以使其适用于生产烃，包括烷烃和生产烃，包括烷烃，使用相同的方法。 本发明的微生物变体具有高电势，以用于通过额外的代谢通量工程改良的菌株，并因此是用于工业生产的烃，包括烷烃是有用的。

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