Abstract:
Sample inspection apparatus, sample inspection method, and sample inspection system are offered which can give a stimulus to a sample (20) held on a film (32) when the sample is inspected by irradiating it with a primary beam (7) (e.g., an electron beam or other charged particle beam) via the film. The apparatus has the film (32), a vacuum chamber (11), primary beam irradiation means, signal detection means (4), and a controller for controlling the operations of the beam irradiation means and signal detection means. The sample (20) is held on a first surface (32a) of the film opened to permit access to the film. The vacuum chamber (11) reduces the pressure of the ambient in contact with a second surface (32b) of the film. The irradiation means irradiates the sample with the primary beam via the film from the second surface side. The detection means (4) detects a secondary signal produced from the sample (20) in response to the irradiation (7).
Abstract:
Many particle-optical apparatus operate with a gas pressure near the sample (118) higher than in the particle-optical column. To avoid leakage into the column a pressure limiting aperture (PLA) with a small diameter is used through which the particle beam (160) leaves the column. Often there is also the need to combine the use of such a column with a path for electro-magnetic radiation, e.g. for the detection of X-rays or when a light-optical microscope is included for observation and/or navigation. Creating a path for this radiation (and optical elements thereof) typically results in an increased working distance for the column, resulting in several disadvantages. The invention offers a solution by placing a window (156) transparent to the electro-magnetic radiation but impervious to gas adjacent to the PLA (140), through which the beam of radiation (170) can enter and/or emerge from the column, to be guided and/or detected further.
Abstract:
An environmental scanning electron microscope which achieves image resolution comparable to that of a conventional SEM is disclosed. A biased ring electrode (28) detects secondary electrons from the specimen and a biased pressure limiting aperture electron detector (50) is also provided to reduce signals emanating from backscattered electrons and to reduce signal noise generated by the electron beam. An optical window system is provided which allows the user to easily switch between the normal environmental SEM electron image (limited to 0.5 mm in diameter) to an optical light view of the sample that covers a field-of-view of up to about 7 to 10 mm. Moreover, the construction of this environmental SEM provides for a take-off angle for the X-ray detectors (EDX detectors) (102) which is comparable to the take-off angle for an EDX detector in a conventional SEM.
Abstract:
Electron microscope provided, in the direction of the longitudinal axis, with at least one electron beam generation system, a condenser and objective lens system, a specimen chamber with a specimen mount, a projection lens system with imaging screen for the purpose of transmission electron microscopy (TEM) and/or an electron detector for the purpose of scanning electron microscopy (SEM). The microscope is used in combination with an externally positioned Raman spectrometer and an associated light source for injecting and extracting, via a window in the microscope wall, a light beam to be directed at the specimen, and specimen-related Raman radiation, respectively. In the specimen chamber, a light beam and Raman radiation guide system is provided with an optical guide to guide the light beam to - and the Raman radiation from - the specimen. The guide system and the specimen mount are displaceable with respect to one another for mutual alignment of the specimen and the optical axis of the Raman spectrometer. The guide system may comprise en objective and lens system positioned at the end of the optical guide. The guide system may be in a fixed position in the specimen chamber next to the longitudinal axis of the microscope, a specimen displacement mechanism displacing the specimen mount from a position in the longitudinal axis of the microscope to a position aligned with the optical axis in the said guide system and vice versa. Equally, the optical guide may be displaceable to a position in which the optical axis running out from the end of the guide coincides with the longitudinal axis of the microscope.
Abstract:
A method and apparatus for aligning a laser beam (216) coincident with a charged particle beam (350). The invention described provides a method for aligning the laser beam through the center of an objective lens (214) and ultimately targeting the eucentric point of a multi-beam system (300). The apparatus takes advantage of components of the laser beam alignment system being positioned within and outside of the vacuum chamber (360) of the charged particle system.
Abstract:
A method of time-resolved pump-probe electron microscopy, comprises the steps of irradiating a sample (1) with a photonic pump pulse (2) being directed on a pump pulse path (3) from a photonic source to the sample (1), irradiating the sample (1) with an electron probe pulse (4) being directed on an electron pulse path (5) from an electron pulse source (10) to the sample (1), wherein the photonic pump pulse (2) and the electron probe pulse (4) arrive at the sample (1) with a predetermined temporal relationship relative to each other, and detecting a sample response to the electron probe pulse (4) irradiation with a detector device (20), wherein the photonic source comprises a photonic lattice structure (30) being arranged adjacent to the electron pulse path (5), and the photonic pump pulse (2) is created by an interaction of the electron probe pulse (4) with the photonic lattice structure (30). Furthermore, an electron microscopy apparatus, configured for time-resolved pump-probe electron microscopy, and a sample supply device (200) for an electron microscopy apparatus (100) are described.
Abstract:
The invention relates to a Correlative Light and Electron Microscope (CLEM), equipped with a TEM column and a light microscope (10), the light microscope fitting between the pole shoes (8A, 8B) of the objective lens of the TEM. To enlarge the acceptance solid angle for enhanced sensitivity a truncated lens is used. It is noted that this does not imply that the lens shows astigmatism (it is not a cylindrical lens). The invention further teaches to make, using the light microscope, a first image with the sample (1) in a first direction. This image will show in one direction a higher (diffraction limited) resolution than in the direction perpendicular thereto, due to the different NA of the lens in the two directions (312 x , 312 y ). By rotating the sample and make a second image, a combined image can be formed showing a better resolution than either of the images in the direction where they show a low NA.
Abstract:
An in situ optical specimen holder is disclosed which allows imaging and analysis during dynamic experimentation. This holder assembly includes a set of focusing and reflection optics along with an environmental cell. Electromagnetic radiation can be used to optically excite the specimen in the presence or absence of fluid and the source of such radiation may be located within the body of the holder itself. The spot size of the irradiation at the specimen surface can be varied, thus exciting only a specific region on the specimen. The window type cell provides a variable fluid path length ranging from the specimen thickness to 500μm. The holder has the provision to continuously circulate fluids over the specimen. The pressure within the cell can be regulated by controlling the flow rate of the fluids and the speed of the pumps.