公开(公告)号：EP0915987A2

公开(公告)日：1999-05-19

申请号：EP98922651.0

申请日：1998-04-06

申请人： DONLAR CORPORATION ,  University of Massachusetts

发明人： TIRRELL, David, A. ,  DEL GROSSO, Michael ,  SIEGMEIER, Rainer ,  BOMMARIUS, Andreas

IPC分类号： C07K14 ,  C12P21

CPC分类号： C12P21/02 ,  C07K14/00

摘要： The invention provides Poly-(α-L-aspartic acid) (= poly-L-α-Asp), Poly-(α-L-glutamic acid) and new copolymers of L-Asp and L-Glu with essentially α-linkages as well as salts of said polymers and copolymers. A method for producing said polyamino acids comprises employing an oligonucleotide construct encoding the sequence of the desired polymer, cloning the oligonucleotide into a plasmid, transformation of host cells with the plasmid and expression of the genetic information. Because of their good biological degradability the new poly-L-α-amino acids can be used as co-builders, sequestering agents and corrosion inhibitors.

公开(公告)号：EP0914415A1

公开(公告)日：1999-05-12

申请号：EP97907592.0

申请日：1997-02-10

申请人： THE ROCKEFELLER UNIVERSITY

发明人： STEINMAN, Ralph, M. ,  BHARDWAJ, Nina ,  SCHULER, Gerald

IPC分类号： C12N5 ,  A61K38

CPC分类号： C12N5/0639 ,  A61K38/00 ,  A61K2039/5154 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2502/11

摘要： We describe an improved method for generating sizable numbers of mature dendritic cells from nonproliferating progenitors in human blood. The first step or 'priming' phase is a culture of T cell depleted mononuclear cells in medium supplemented with GM-CSF and IL-4 to produce immature dendritic cells. The second step or 'differentiation' phase requires the exposure to dendritic cell maturation factor such as monocyte conditioned medium. Using this two-step approach, substantial yields are obtained. The dendritic cells derived from this method have all the features of mature cells. They include a stellate cell shape, nonadherence to plastic, and very strong T cell stimulatory activity. The mature dendritic cells produced according to this invention are useful for activating T cells.

公开(公告)号：EP0912892A1

公开(公告)日：1999-05-06

申请号：EP97928806.0

申请日：1997-05-29

申请人： Biodx

发明人： TAYLOR, D., Lansing

IPC分类号： B01J19 ,  C12M1 ,  G01N21 ,  G01N33

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00351 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00704 ,  B01J2219/00707 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5047 ,  G01N33/5058 ,  G01N33/5438 ,  G01N33/56966 ,  G01N2021/6471

摘要： The present invention discloses devices and methods of performing high-throughput screening of the physiological response of cells to biologically active compounds and methods of combining high-throughput with high-content spatial information at the cellular and subcellular level as well as temporal information about changes in physiological, biochemical and molecular activities. The present invention allows multiple types of cell interactions to be studied simultaneously by combining multicolor luminescence reading, microfluidic delivery, and environmental control of living cells in non-uniform micro-patterned arrays.

公开(公告)号：EP0912605A1

公开(公告)日：1999-05-06

申请号：EP97931724.0

申请日：1997-07-07

申请人： Fischer, Lutz

发明人： FISCHER, Lutz ,  PEISSKER, Fabian

IPC分类号： G01N33 ,  B01J20 ,  C07K14 ,  C07K16 ,  C07K17 ,  C12N9 ,  C12N11 ,  G01N30

CPC分类号： B01J20/26 ,  B01D15/3852 ,  B01J20/268 ,  B01J20/285 ,  C07K14/001 ,  C07K16/00 ,  C12N9/00 ,  C12N9/2445 ,  C12N9/6424 ,  C12N9/96 ,  C12Y302/01021 ,  G01N2600/00 ,  G01N2650/00

摘要： The invention relates to imprint polypeptides covalently cross-linked and having a fixed and stabilised arrangement, process for the preparation and uses thereof. The properties of said imprint polypeptides (VIP) covalently cross-linked are present in aqueous and in organic solvent systems, and can therefore be used in both types of system.

公开(公告)号：EP0897568A1

公开(公告)日：1999-02-24

申请号：EP97924731.0

申请日：1997-05-15

申请人： THE PERKIN-ELMER CORPORATION

发明人： SHARAF, Muhammad, A.

IPC分类号： C12N15 ,  C12Q1 ,  G01N21 ,  G01N27 ,  G01N30 ,  G01N33 ,  G06F17

CPC分类号： G01N30/8665 ,  G01N2201/129

摘要： A signal processing method in a processor is provided for performing a multicomponent analysis of a signal resulting from a spectral response of a mixture comprising a plurality of spectrally resolvable molecular species. The method provides both a determination of a concentration estimate and a statistical confidence interval for each species. In the method, a data vector d is received from a multichannel detector, data vector d having a length nc, nc being the number of detector channels being monitored. A calibration matrix K having nc rows and np columns is provided wherein nc is larger than np, np being the number of spectrally resolvable molecular species. Next, a concentration estimate vector c having length np is determined. Finally, a confidence interval CIi for each of the elements of the concentration estimate vector is determined according to the expression CIi = ci ± (varcovar(cii))1/2Q¿(cl, nf)? where Q is the critical value of a statistical distribution for a given level of confidence, cl, and a given number of degrees of freedom nf where nf = nc-np. The invention further includes a program storage device embodying the method; a DNA sequencing process employing the method; and, an apparatus for carrying out the method.

公开(公告)号：EP0888456A2

公开(公告)日：1999-01-07

申请号：EP97916850.0

申请日：1997-03-19

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE DEPARTMENT OF HEALTH & HUMAN SERVICES

发明人： SCHLOM, Jeffrey ,  TSANG, Kwong-yok ,  ZAREMBA, Sam

IPC分类号： A61K39 ,  C07H21 ,  C07K14 ,  C12N5 ,  C12N9 ,  C12N15 ,  C12Q1 ,  A61K35 ,  A61K38

CPC分类号： A61K39/0011 ,  A61K38/00 ,  A61K39/00 ,  A61K2035/124 ,  A61K2039/5154 ,  A61K2039/5156 ,  A61K2039/5158 ,  A61K2039/5254 ,  A61K2039/5256 ,  A61K2039/53 ,  A61K2039/55522 ,  A61K2039/55527 ,  C12N5/0636 ,  C12N9/6445 ,  C12N2710/24143 ,  Y10S435/975

摘要： The invention is a prostate specific antigen oligo-epitope peptide which comprises more than one PSA epitope peptide, which conforms to one or more human HLA class I motifs. The prostate specific antigen oligo-epitope peptide in combination with various HLA-class I molecules or interactions with various T-cell receptors elicits PSA specific cellular immune responses. The prostate specific antigen oligo-epitope peptide is useful as an immunogen in the prevention or treatment of prostatic cancer, in the inhibition of prostatic cancer cells and in the establishment and characterization of PSA-specific cytotoxic T-cell lines.

公开(公告)号：EP0885229A1

公开(公告)日：1998-12-23

申请号：EP97914792.0

申请日：1997-02-20

申请人： CEPHALON, INC.

发明人： HUDKINS, Robert, L. ,  DIEBOLD, James, L. ,  KNIGHT, Ernest, Jr.

IPC分类号： A61K31 ,  A61P5 ,  A61P25 ,  A61P31 ,  A61P35 ,  A61P37 ,  C07D487 ,  C07D491 ,  C07D493 ,  C07D495

CPC分类号： C07D487/04 ,  C07D487/14 ,  C07D493/14 ,  C07D495/14

摘要： Disclosed in this patent document are synthetic, biologically active molecules referred to as fused pyrrolo(2,3-c)carbazole-6-ones. These molecules are represented by general formulae (I) and (II). Methods for making and using the fused pyrrolo(2,3-c)carbazole-6-ones are also disclosed.

公开(公告)号：EP0885013A1

公开(公告)日：1998-12-23

申请号：EP97922838.0

申请日：1997-04-14

申请人： Mayr, Anton, Prof. Dr.med.vet. Dr.h.c.mult.

发明人： Mayr, Anton, Prof. Dr.med.vet. Dr.h.c.mult.

IPC分类号： A61K45 ,  A61K31 ,  A61K35 ,  A61K39 ,  A61P1 ,  A61P17 ,  A61P25 ,  A61P31 ,  A61P35 ,  A61P39

CPC分类号： A61K35/76 ,  C12N2710/24232

摘要： The use is disclosed of multipotent parapox immunity inducers from attenuated, non-immunogenic pox or parapox viruses (pox inducers) to produce medicaments with new indication ranges in human medicine.

公开(公告)号：EP0880701A1

公开(公告)日：1998-12-02

申请号：EP96929056.0

申请日：1996-08-21

申请人： THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

发明人： KOHN, Leonard ,  SINGER, Dinah, S. ,  SAJI, Motoyasu ,  GIULIANI, Cesidio ,  SHONG, Minho ,  SUZUKI, Koichi ,  OHMORI, Masayuki

IPC分类号： C12N15 ,  A61K31 ,  A61K48 ,  A61P37 ,  C07K14 ,  C07K16 ,  C12P21 ,  C12Q1 ,  G01N33 ,  C12R1

CPC分类号： G01N33/68 ,  C07K14/4702 ,  C07K14/4713 ,  C07K14/70539 ,  C12Q1/68 ,  C12Q1/6897

摘要： The present invention provides methods for treating autoimmune diseases in mammals and for preventing or treating transplantation rejection in a transplant recipient. The methods of treatment involve the use of drugs capable of suppressing expression of MHC Class I molecules. In particular the use of the drug methimazole to suppress expression of MHC Class I molecules in the treatment of autoimmune diseases and the prevention or treatment of rejection in a transplant recipient is disclosed. In addition in vivo and in vitro assays are provided for the assessment and development of drugs capable of suppressing MHC Class I molecules. In addition, this invention relates to nucleic acid and amino acid sequences for proteins capable of modulating MHC Class I expression.

公开(公告)号：EP0877760A1

公开(公告)日：1998-11-18

申请号：EP97905709.0

申请日：1997-01-30

申请人： Sunol Molecular Corporation

发明人： RHODE, Peter, R. ,  JIAO, Jin-An ,  BURKHARDT, Martin ,  WONG, Hing, C.

IPC分类号： A01K67 ,  A61K35 ,  A61K38 ,  A61K39 ,  A61K48 ,  A61P3 ,  A61P21 ,  A61P25 ,  A61P29 ,  A61P37 ,  A61P43 ,  C07K14 ,  C07K19 ,  C12N5 ,  C12N15 ,  C12P21 ,  C12Q1

CPC分类号： C07K14/70539 ,  A01K2217/05 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/6043 ,  A61K2039/622 ,  C07K2319/00 ,  C07K2319/02

摘要： The present invention relates to novel complexes of major histocompatibility complex (MHC) molecules and uses of such complexes. In one aspect, the invention relates to loaded MHC complexes that include at least one MHC molecule with a peptide-binding groove and a presenting peptide non-covalently linked to the MHC protein. In another aspect, the invention features single chain MHC class II peptide fusion complexes with a presenting peptide covalently linked to the peptide binding grove of the complex. MHC complexes of the invention are useful for a variety of applications including: 1) in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, and 2) methods for suppressing or inducing an immune response in a mammal.