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61.发明授权Gene regulation cassettes, exüressopm vectors containing the same and microorganisms transformed with the same 失效基因调节盒，含有它们的表达载体和微生物转化有这些。
公开(公告)号：EP0355625B1
公开(公告)日：1995-05-10
申请号：EP89114908.0
申请日：1989-08-11
CPC分类号： C07K14/53 , C07K14/545 , C12N15/73
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公开(公告)号：EP0547873A3
公开(公告)日：1994-08-31
申请号：EP92311446.6
申请日：1992-12-15
摘要： A novel translational activating sequence is disclosed, said translational activating sequence having an oligonucleotide sequence comprising : Recombinant DNA vectors comprising the novel translational activating sequence and utilities thereof are also disclosed.
摘要翻译： 公开了一种新的翻译活化序列，所述翻译活化序列具有寡核苷酸序列，所述寡核苷酸序列包含：还公开了包含新型翻译活化序列的重组DNA载体及其应用。
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公开(公告)号：EP0343132B1
公开(公告)日：1994-08-17
申请号：EP89850141.6
申请日：1989-05-02
申请人： FERROPAS AG
CPC分类号： C07K14/005 , C12N2740/16222
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公开(公告)号：EP0547873A2
公开(公告)日：1993-06-23
申请号：EP92311446.6
申请日：1992-12-15
摘要： A novel translational activating sequence is disclosed, said translational activating sequence having an oligonucleotide sequence comprising : Recombinant DNA vectors comprising the novel translational activating sequence and utilities thereof are also disclosed.
摘要翻译： 公开了一种新的翻译活化序列，所述翻译活化序列具有寡核苷酸序列，其包含：包含新的翻译活化序列及其用途的重组DNA载体也被公开。
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公开(公告)号：EP0471514A2
公开(公告)日：1992-02-19
申请号：EP91307325.0
申请日：1991-08-09
发明人： Belagaje, Rama M. , Hershberger, Charles Lee , Hsiung, Hansen Maxwell , Rosteck, Paul Robert, Jr. , Sterner, Jane Larowe
IPC分类号： C12N15/73
摘要： The present invention concerns novel DNA compounds which function as transcriptional activating sequences. Recombinant DNA expression vectors which contain the transcriptional activating sequences and host cells transformed with these expression vectors are also provided. When properly positioned in an expression vector, the novel transcriptional activating sequences enable regulatable expression of an operably linked gene and enhance the structural stability of the expression vector.
摘要翻译： 本发明涉及用作转录激活序列的新型DNA化合物。 还提供了含有转录激活序列和用这些表达载体转化的宿主细胞的重组DNA表达载体。 当正确位于表达载体中时，新的转录激活序列使得可操纵连接的基因的可调节表达增强了表达载体的结构稳定性。
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公开(公告)号：EP0210279A4
公开(公告)日：1987-06-19
申请号：EP86900852
申请日：1986-01-24
申请人： SAGAMI CHEM RES , CENTRAL GLASS CO LTD , HODOGAYA CHEMICAL CO LTD , NIPPON SODA CO , NISSAN CHEMICAL IND LTD , TOYO SODA MFG CO LTD
发明人： MIYAKE TOSHIO , HIBINO YASUO , KOBAYASHI YOICHI , WATABE KEN , OMORI MUNEKI , MIKI TETSUZO , YOKOYAMA MIDORI , MATSUMOTO REIKO , WATANABE KAZUO , NUMAO NAGANORI
CPC分类号： C12N9/6462 , C12N15/73 , C12Y304/21073
摘要： Stabilized human prourokinase-like polypeptides wherein the 135th lysine from the N-terminal of human prourokinase is substituted by an amino acid other than basic amino acids, the 157th phenylalanine is substituted by an acidic amino acid, or both of the amino acids are substituted as described above; DNA segment which codes said polypeptides; plasmid which contains said DNA segment; E. coli transformed by said plasmid; and a process for preparing said polypeptide by using said E. coli. The polypeptides are stable against proteases such as plasmin, thrombin, trypsin, etc., thus being easily purified and collected in a pure form. When applied to a living body, the polypeptides are expected to provide a persistent fibrinolytic activity.
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67.发明公开The use of bacteriophage lambda PL promoter to produce a functional polypeptide in streptomyces 失效使用λ噬菌体PL启动子用于产生在链霉菌中的功能性多肽。
公开(公告)号：EP0177228A1
公开(公告)日：1986-04-09
申请号：EP85306666.0
申请日：1985-09-19
CPC分类号： C12N15/73 , C12N15/76 , Y10S435/886 , Y10S435/889 , Y10S435/896
摘要： (57) The invention provides a method and cloning vehicle for the expression of a functional polypeptide in Streptomyces. A recombinant DNA cloning vehicle was genetically- engineered to bring the expression of a functional polypeptide gene under the control of the Escherichia coli bacteriophage λp L promoter.
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68.发明公开Plasmids containing lambda Pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, hosts containing the plasmids and related methods 失效和λPL含有启动子的质粒，以及经改造的限制性位点便于更换一个核糖体结合位点的，含有主机和相应的方法的质粒。
公开(公告)号：EP0173280A1
公开(公告)日：1986-03-05
申请号：EP85110683.1
申请日：1985-08-26
发明人： Aviv, Haim , Bartfeld, Daniel , Gorecki, Marian , Hartman, Jacob R. , Kanner, Dov , Levanon, Avigdor , Locker-Giladi, Hilla , Oppenheim, Amos B.
CPC分类号： C12N9/0089 , A23K20/168 , A61K38/446 , C07K14/61 , C07K14/775 , C12N15/69 , C12N15/73
摘要： An improved vector which upon introduction into a suitable host containing the thermolabile repressor C 1 renders the host capable of effecting expression of a desired gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: the promoter and operator P L O L from lambda bacteriophage; the N utilization site; a first restriction enzyme site which follows thereafter; a ribosomal binding site; an ATG Initiation codon or DNA which is converted Into an ATG initiation codon upon insertion of the desired gene into the vector, a second restriction enzme site for inserting the gene in phase with the ATG initiation codon; an origin of replication and either a gene associated with a selectable or identifiable phenotypic trait manifested when the vector is present In the host or a fragment designated ci 434 which includes the gene for the cl 343 repressor protein and its associated promoter and operator. The distance between the 3' end of P L O L and the 5' end of the N utilization site is less than about 80 base pairs. The distance between the 3' end of the N utilization site and the 5' end of the ribosomal binding site is less than about 300 base pairs.
Vectors of the invention may also include a T,T 2 rRNA transcription termination sequence located 3' of the second restriction enzyme site. The inventive vectors may have origin of replications capable of autonomous production in the host of at least 400 constitutive copies. Plasmids have been constructed from the vectors and used to produce bovine, chicken, porcine and human growth hormones. human apolipoprotein E and human superoxide dismutase and ana- loges thereof In host cells.
Such superoxide dismutase or analogs thereof may be used to catalyze the reduction of superoxide radicais, reduce reperfusion injury, prolong the survival time of isolated organs and prevent neurological InJury.
Enzymatically active eucaryotic superoxide dismutase or an analog thereof may also be produced by an improved method of this invention which employs a production medium having a concentration of Cu ++ greater than about about 2 ppm.
. The invention also concerns improved methods of ro- covering purified enzymatically active eucaryotic superoxide dismutase or analogs thereof.摘要翻译： （1）向做了引入合适的细菌宿主细胞contg。 不耐热阻遏C（I）使宿主细胞能，当温度。 被升高到使阻遏物失活 - 影响由该基因编码插入以产生多肽（Ⅰ）载体中的目的基因的表达的，电平是新当其包含双链DNA分子包括在5“到 3“顺序。 （A）的DNA序列contg。 从拉姆达噬菌体的启动子和操纵P（L）O（L）; （B）一个氮利用的网站结合抗终止子N蛋白; （C）的第一限制性内切酶位点允许置换的DNA序列contg的。 核糖体结合位点后，继; （D）的DNA序列contg。 用于制备能够结合在宿主细胞中的核糖体的所需基因的mRNA核糖体结合位点; （E）ATG起始密码子或转化成所希望的基因向载体的插入密码子的DNA序列; 和（f）的第二限制性内切酶位点用于将基因导入与ATG起始密码子相所需载体; 和（g）的DNA序列contg。 从能够在宿主细胞中自主复制的细菌质粒的复制起点; 和选择，DNA序列contg。 与禅意当矢量存在于宿主细胞中，或DNA序列contg可选择或可识别的表型性状相关的基因。 片段C（I）434，包括基因的C（I）434阻遏蛋白和其相关联的启动子和操纵基因序列。
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69.发明公开
公开(公告)号：EP0095934A2
公开(公告)日：1983-12-07
申请号：EP83303125.5
申请日：1983-06-01
摘要： The DNA of a novel bacteriophage is characterized in that (1) in the upstream portion from the late promoter necessary for the transcription of genetic information for the biosynthesis of coat proteins up to the cohesive end, there is no endonuclease cleavage site or there are endonuclease cleavage sites permissible in number for the reconstruction of a DNA molecule when the DNA is cleaved with endonuclease into fragments and then these fragments are rejoined with DNA ligase, and (2) in the downstream portion from the late promoter down to the cohesive end, there is one or more endonuclease cleavage sites.
摘要翻译： 新型噬菌体的DNA的特征在于，（1）在后期启动子的上游部分，用于转录用于生物合成外壳蛋白直到粘性末端的遗传信息，没有内切核酸酶切割位点或有内切核酸酶 当DNA用内切核酸酶切割成片段时，重组DNA分子的数目允许的切割位点，然后这些片段用DNA连接酶重新连接，和（2）在后期启动子的下游部分到粘性末端，那里 是一个或多个内切核酸酶切割位点。
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70.发明公开Improved vectors and methods for making such vectors and for expressing cloned genes 无效改进的载体，使这些载体用于表达克隆的基因的和方法。
公开(公告)号：EP0041767A2
公开(公告)日：1981-12-16
申请号：EP81301413.1
申请日：1981-04-01
申请人： BIOGEN, INC.
IPC分类号： C12N15/52 , A61K39/135 , C07K15/00 , C12N15/73
CPC分类号： C07K14/005 , A61K38/00 , C07K14/565 , C07K2319/00 , C07K2319/02 , C07K2319/40 , C07K2319/75 , C12N15/52 , C12N15/73 , C12N2710/22022
摘要： Improved vectors and methods for expressing cloned genes of prokaryotic or eukaryotic origin and methods of making such vectors are disclosed, the improved vectors comprising promoters and operators from λ phages and preferably do not include an active cro gene or an active N gene, the vectors having at least one endonuclease recognition site forcloning desired genes less than about 300 base pairs from the promoters and operators and being useful, as are methods utilizing the vectors, in producing a wide variety of prokaryotic, eukaryotic and viral polypeptides, hormones, enzymes, antigens, proteins and amino acids.
摘要翻译： 改进的用于表达的原核或真核来源的克隆的基因，使搜索矢量的方法的载体和方法是圆盘游离缺失，改进的载体，其包括从拉姆达噬菌体启动子和运营商和优选地不包括在活性CRO基因或活性的N-基因，具有该矢量 至少一种内切核酸酶识别位点小于从启动子约300个碱基对和运营商和克隆目的基因是有用的，因为是利用方法的载体，在制造各种各样的原核，真核和病毒多肽激素，酶，抗原的 ，蛋白质和氨基酸。
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