公开(公告)号：EP3007819A4

公开(公告)日：2017-01-18

申请号：EP14810860

申请日：2014-06-13

申请人： ADVANCED LIQUID LOGIC INC

发明人： PAMULA VAMSEE K ,  SRINIVASAN VIJAY

IPC分类号： B01J19/00 ,  B01L3/00 ,  G01N1/28

CPC分类号： G01N27/44791 ,  B01J19/0093 ,  B01J2219/0084 ,  B01J2219/00853 ,  B01L3/502784 ,  B01L3/502792 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/165 ,  B01L2300/1827 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N27/44704

公开(公告)号：EP2516669B1

公开(公告)日：2016-10-12

申请号：EP10842658.6

申请日：2010-12-17

申请人： Advanced Liquid Logic, Inc.

发明人： ECKHARDT, Allen, E. ,  GRAHAM, Carrie ,  SISTA, Ramakrishna ,  PAMULA, Vamsee, K. ,  WINGER, Theodore ,  WANG, Tong ,  XIONG, Yalin ,  WU, Ning ,  SAHA, Sanjay ,  RAOLJI, Gajendrasinh, Balvantsinh ,  DAYAM, Raveendra

IPC分类号： B01L3/00 ,  C07D311/18 ,  C07D311/20 ,  C07H15/203 ,  C12Q1/25 ,  C12Q1/34 ,  G01N33/543 ,  C07D311/16 ,  C07D405/12 ,  C07H17/075 ,  G01N21/64 ,  C07D413/12

CPC分类号： C12Q1/40 ,  B01L3/502784 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07D311/16 ,  C07D311/18 ,  C07D311/20 ,  C07D405/12 ,  C07D413/12 ,  C07H15/203 ,  C07H17/075 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/54 ,  C12Y302/01023 ,  G01N21/6452 ,  G01N33/5438 ,  G01N2333/916 ,  G01N2333/926 ,  G01N2333/938 ,  G01N2800/04

摘要： The invention is directed to droplet actuator devices and assay methods. The method may include immobilization of the enzymatic substrate including forming an inclusion complex with the substrate within an aqueous environment in contact with an oil.

摘要翻译： 本发明涉及液滴致动器装置和测定方法。 本发明包括进行测定的测定方法，其包括将样品与伞形叶酸衍生物组合，其中所述样品潜在地包含能够切割所述伞形酮基衍生物的酶，并且其中所述伞形载体衍生物包含用一个或多个修饰部分修饰的伞形酮核心。

公开(公告)号：EP2237955A4

公开(公告)日：2016-04-20

申请号：EP08868761

申请日：2008-12-23

申请人： ADVANCED LIQUID LOGIC INC

发明人： SRINIVASAN VIJAY ,  POLLACK MICHAEL ,  PAMULA VAMSEE ,  HUA ZHISHAN ,  SUDARSAN ARJUN ,  PAIK PHILIP

IPC分类号： B41J2/06 ,  B01F13/00 ,  B01L3/00 ,  B01L3/02 ,  B41J29/38 ,  F04B19/00

CPC分类号： B01L3/52 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/0241 ,  B01L3/502792 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/143 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/06 ,  B01L2400/0688 ,  F04B19/006

摘要： A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.

公开(公告)号：EP2997126A2

公开(公告)日：2016-03-23

申请号：EP14798475.1

申请日：2014-05-16

申请人： Advanced Liquid Logic, Inc.

发明人： PUNNAMARAJU, Srikoundinya ,  SANDAHL, Melissa, A. ,  HARRINGTON, John, J. ,  ECKHARDT, Allen, E. ,  HUA, Zhishan ,  POLLACK, Michael, G.

IPC分类号： C12M1/42

CPC分类号： C12N15/87 ,  C12M33/00 ,  C12M35/02 ,  C12N13/00

摘要： The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.

摘要翻译： 本发明提供了一种液滴致动器，其设计用于在液滴中对细胞进行电穿孔。 本发明还提供了用于在液滴致动器上以液滴的细胞进行电穿孔的方法和系统。

公开(公告)号：EP2837692A1

公开(公告)日：2015-02-18

申请号：EP14186092.4

申请日：2008-03-23

申请人： Advanced Liquid Logic, Inc.

发明人： Eckhardt, Allen ,  Pamula, Vamsee

IPC分类号： C12Q1/34 ,  B01F13/08 ,  C07H15/04

CPC分类号： G01N21/6486 ,  B01F13/0809 ,  C07H15/04 ,  C12Q1/34 ,  G01N2333/928 ,  G01N2800/042

摘要： A method of conducting a droplet-based enzymatic assay is provided. The method generally makes use of a droplet actuator. A droplet comprising an enzyme of interest is provided on the droplet actuator along with a droplet comprising a substrate which is potentially modified in the presence of the enzyme. The method involves executing droplet operations on the droplet actuator to combine the droplets, thereby yielding an assay droplet, and detecting modification of the substrate by the enzyme in the assay droplet on the droplet actuator. The enzyme of interest may, for example, be a potentially mutated or improperly folded enzyme exhibiting altered enzyme activity as compared to a corresponding normal enzyme.

摘要翻译： 提供了一种进行基于液滴的酶测定的方法。 该方法通常使用液滴致动器。 包含感兴趣的酶的液滴与包含底物的液滴一起提供在液滴致动器上，该底物在酶的存在下可能被修饰。 该方法涉及在液滴致动器上执行液滴操作以组合液滴，从而产生测定液滴，并且通过液滴致动器上的测定液滴中的酶检测底物的修饰。 感兴趣的酶可以是例如与相应的正常酶相比显示出酶活性变化的潜在的突变或不正确折叠的酶。

公开(公告)号：EP2809784A1

公开(公告)日：2014-12-10

申请号：EP13742978.3

申请日：2013-01-22

申请人： Advanced Liquid Logic, Inc. ,  Leitner, Thomas

发明人： LEITNER, Thomas

IPC分类号： C12N15/11 ,  C12Q1/68

CPC分类号： C12Q1/703 ,  C12Q2600/112

摘要： The present invention relates to amplification oligonucleotides (primers) and detection probes for the detection of human immunodeficiency virus type-1 (HIV-1) in a test sample. The primers and probes may be used in an assay for the detection and/or quantitation of HIV-1 nucleic acid. In various embodiments, the primers and detection probes may be used for real-time reverse transcription polymerase chain reaction (RT-PCR) assays for detection of HIV-1 nucleic acid. The detection probes may, for example, be fluorescently-labeled TaqMan® probes. In one embodiment, amplification primers and probes may be specific for the pol region of the HIV-1 genome. In another embodiment, the amplification primers and probes may be specific for the env region of the HIV-1 genome. "Cocktails" of multiple amplification primers and/or probes may be used to achieve maximal coverage of different HIV-1 subtypes.

公开(公告)号：EP2705374A4

公开(公告)日：2014-11-12

申请号：EP12779928

申请日：2012-05-01

申请人： ADVANCED LIQUID LOGIC INC

发明人： BORT DONOVAN ,  GRAHAM CARRIE ,  PAMULA VAMSEE ,  POLLACK MICHAEL ,  SISTA RAMAKRISHNA ,  SRINIVASAN VIJAY

IPC分类号： G01N35/08 ,  B01F3/08 ,  C12Q1/68 ,  G01N21/00

CPC分类号： G01N27/44791 ,  B01F1/0027 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2001/0055 ,  B01F2215/0037 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  B81B1/004

摘要： A droplet actuator (110) comprising: one or more substrates (114, 116) arranged to form a droplet operations gap, the droplet operations gap comprising a region of gap height transition in which the gap height of the droplet operations gap transitions from a first gap height to a second gap height; and electrodes associated with the one or more substrates arranged for conducting droplet operations, the electrodes comprising a transition electrode which traverses the region of gap height transition. The electrodes may comprise electrowetting electrodes. The transition electrode may be flanked by first and second sets of electrodes having first and second footprint areas. The first footprint area may be greater than the second footprint area. The first set of electrodes may be arranged in a region of greater gap height and the second set of electrodes may be arranged in the region of lesser gap height.

公开(公告)号：EP2719449A1

公开(公告)日：2014-04-16

申请号：EP13192675.0

申请日：2012-05-01

申请人： Advanced Liquid Logic, Inc.

发明人： Bort, Donovan ,  Graham, Carrie ,  Pamula, Vamsee ,  Pollack, Michael ,  Sista, Ramakrishna ,  Srinivasan, Vijay

IPC分类号： B01F13/00 ,  B01L3/00

CPC分类号： G01N27/44791 ,  B01F1/0027 ,  B01F13/0071 ,  B01F13/0076 ,  B01F2001/0055 ,  B01F2215/0037 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  B81B1/004

摘要： A droplet actuator (110) comprising: one or more substrates (114, 116) arranged to form a droplet operations gap, the droplet operations gap comprising a region of gap height transition in which the gap height of the droplet operations gap transitions from a first gap height to a second gap height; and electrodes associated with the one or more substrates arranged for conducting droplet operations, the electrodes comprising a transition electrode which traverses the region of gap height transition. The electrodes may comprise electrowetting electrodes. The transition electrode may be flanked by first and second sets of electrodes having first and second footprint areas. The first footprint area may be greater than the second footprint area. The first set of electrodes may be arranged in a region of greater gap height and the second set of electrodes may be arranged in the region of lesser gap height.

摘要翻译： 液滴致动器（110）包括：布置成形成液滴操作间隙的一个或多个基板（114,116），所述液滴操作间隙包括间隙高度过渡区域，其中液滴操作间隙的间隙高度从第一 间隙高度到第二间隙高度; 以及与布置用于进行液滴操作的所述一个或多个基板相关联的电极，所述电极包括穿过间隙高度转变区域的过渡电极。 电极可以包括电润湿电极。 过渡电极可以由具有第一和第二覆盖区域的第一和第二组电极的侧面。 第一个足迹区域可能大于第二个足迹区域。 第一组电极可以布置在较大间隙高度的区域中，并且第二组电极可以布置在较小间隙高度的区域中。

公开(公告)号：EP2286228A4

公开(公告)日：2014-04-09

申请号：EP09747752

申请日：2009-05-18

申请人： ADVANCED LIQUID LOGIC INC

发明人： SISTA RAMAKRISHNAN ,  PAMULA VAMSEE ,  SUDARSAN ARJUN ,  SRINIVASAN VIJAY ,  THWAR PRASANNA

IPC分类号： G01N33/543 ,  B01L3/00 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/68

CPC分类号： B01L3/502792 ,  B01L3/502761 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/089 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0487 ,  G01N1/4077 ,  G01N35/0098 ,  G01N2035/00564 ,  Y10T436/25

摘要： The invention provides droplet actuators and droplet actuator techniques. Among other things, the droplet actuators and methods are useful for manipulating beads on a droplet actuator, such as conducting droplet operations using bead-containing droplets on a droplet actuator. For example, beads may be manipulated on a droplet actuator in the context of executing a sample preparation protocol and/or an assay protocol. An output of the methods of the invention may be beads prepared for execution of an assay protocol. Another output of the methods of the invention may be results of an assay protocol executed using beads. Among the methods described herein are methods of concentrating beads in droplets, methods of washing beads, methods of suspending beads, methods of separating beads, methods of localizing beads within a droplet, methods of forming emulsions in which droplets include beads, methods of loading beads into a droplet operations gap of a droplet actuator, methods of organizing beads in a monolayer, and methods of capturing, trapping or restraining beads.

公开(公告)号：EP2111554B1

公开(公告)日：2013-05-08

申请号：EP08729498.9

申请日：2008-02-11

申请人： Advanced Liquid Logic, Inc.

发明人： SISTA, Ramakrishna ,  PAMULA, Vamsee K. ,  POLLACK, Michael G. ,  SRINIVASAN, Vijay ,  ECKHARDT, Allen E.

IPC分类号： G01N33/543 ,  B01L3/00

CPC分类号： G01N33/54326 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0427 ,  B01L2400/043 ,  C12M25/01 ,  C12M25/16 ,  G01N27/44717 ,  G01N27/44786 ,  G01N33/54386 ,  G01N35/0098 ,  G01N2035/1046

摘要： A method of assembling a droplet actuator assembly (1400) comprises: attaching to a mount structure (1410) a substrate (1434) including an electrode emitting an electric field (1438) affecting a droplet present in the droplet actuator assembly and a magnet assembly (1420) configured to accommodate a magnet (1428) emitting a magnetic field affecting a magnetically responsive bead present in the droplet actuator assembly. At least one of the substrate (1434) and the magnet assembly (1420) are removably attached to the mount structure (1410). Attaching the substrate (1434) may further comprise selecting the substrate from among a plurality of substrates (1434) configured to attach to the mount structure (1410) and including different electric field emission characteristics, and/or selecting the magnet assembly (1420) from among a plurality of magnetic assemblies (1420), each magnet assembly configured to attach to the mount structure (1410) and including different magnet properties.