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公开(公告)号：EP2561064B1
公开(公告)日：2017-10-18
申请号：EP11714788.4
申请日：2011-04-19
申请人： DSM IP Assets B.V.
发明人： KLAASSEN, Paul , GIELESEN, Bianca Elisabeth Maria , SUYLEKOM, VAN, Gijsberdina Pieternella , HEIJNE, Wilbert Herman Marie
IPC分类号： C12N1/36 , C07K14/395
CPC分类号： C12P7/10 , C07K14/395 , C12N1/36 , C12N9/0004 , C12N9/0006 , C12N9/10 , C12N9/1205 , C12N9/16 , C12N9/2405 , C12N9/2411 , C12N9/2437 , C12N9/2468 , C12N9/248 , C12N9/50 , C12N9/88 , C12N9/90 , C12N9/92 , C12P5/026 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/44 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P13/005 , C12P13/08 , C12P13/12 , C12P13/20 , C12P13/227 , C12P17/10 , C12P35/00 , C12Y101/01 , C12Y207/01016 , C12Y501/03004 , C12Y503/01004 , Y02E50/16 , Y02E50/17 , Y02E50/343
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2.发明公开CELL SUITABLE FOR FERMENTATION OF A MIXED SUGAR COMPOSITION 有权Zelle zur Fermentierung einer gemischten Zuckerzusammensetzung
公开(公告)号：EP2561064A1
公开(公告)日：2013-02-27
申请号：EP11714788.4
申请日：2011-04-19
申请人： DSM IP Assets B.V.
发明人： KLAASSEN, Paul , GIELESEN, Bianca Elisabeth Maria , SUYLEKOM, VAN, Gijsberdina Pieternella , HEIJNE, Wilbert Herman Marie
IPC分类号： C12N1/36 , C07K14/395
CPC分类号： C12P7/10 , C07K14/395 , C12N1/36 , C12N9/0004 , C12N9/0006 , C12N9/10 , C12N9/1205 , C12N9/16 , C12N9/2405 , C12N9/2411 , C12N9/2437 , C12N9/2468 , C12N9/248 , C12N9/50 , C12N9/88 , C12N9/90 , C12N9/92 , C12P5/026 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/44 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P13/005 , C12P13/08 , C12P13/12 , C12P13/20 , C12P13/227 , C12P17/10 , C12P35/00 , C12Y101/01 , C12Y207/01016 , C12Y501/03004 , C12Y503/01004 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol 10 dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.
摘要翻译： 本发明涉及一种生产能够转化阿拉伯糖的细胞的方法，包括以下步骤：a）引入不能转化阿拉伯糖的宿主菌株，AraA，araB和araD基因，该细胞被命名为构建的细胞 ; b）使构建的细胞进行自适应进化，直到获得转化阿拉伯糖的细胞，c）任选地，使第一阿拉伯糖转化细胞经受适应性进化以改善阿拉伯糖转化; 步骤b）或c）中产生的细胞被指定为第一阿拉伯糖转化细胞; d）分析第一个阿拉伯糖转化细胞和构建的细胞的全基因组或部分基因组; e）鉴定第一个阿拉伯糖转化细胞中的单核苷酸多态性（SNP）; 和f）利用SNP的信息合理设计能够转化阿拉伯糖的细胞; g）能够转化步骤f）中设计的阿拉伯糖的细胞的构建。
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公开(公告)号：EP2250263A1
公开(公告)日：2010-11-17
申请号：EP09718278.6
申请日：2009-03-05
申请人： DSM IP Assets B.V.
发明人： KLAASSEN, Paul , LAAN, VAN DER, Jan Metske , GIELESEN, Bianca Elisabeth Maria , SUYLEKOM, VAN, Gijsberdina Pieternella
摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.
摘要翻译： 本发明涉及包含编码木糖异构酶的核苷酸序列的细胞，其中木糖异构酶的氨基酸序列与SEQ ID NO：3中所示的氨基酸序列具有至少约70％的序列同一性，并且其中核苷酸 序列对宿主而言是异源的。 本发明的细胞可用于生产发酵产物如乙醇的方法中。 这种方法可以包括用本发明的细胞发酵含有木糖来源的培养基，使得细胞将木糖发酵成发酵产物。
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公开(公告)号：EP2250262A1
公开(公告)日：2010-11-17
申请号：EP09718191.1
申请日：2009-03-05
申请人： DSM IP Assets B.V.
发明人： KLAASSEN, Paul , LAAN, VAN DER, Jan Metske , GIELESEN, Bianca Elisabeth Maria , SUYLEKOM, VAN, Gijsberdina Pieternella
摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. A cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.
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公开(公告)号：EP2250262B1
公开(公告)日：2015-07-29
申请号：EP09718191.1
申请日：2009-03-05
申请人： DSM IP Assets B.V.
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6.发明公开
公开(公告)号：EP2560988A1
公开(公告)日：2013-02-27
申请号：EP11714789.2
申请日：2011-04-19
申请人： DSM IP Assets B.V.
发明人： KLAASSEN, Paul , GIELESEN, Bianca Elisabeth Maria , HEIJNE, Wilbert Herman Marie , SUYLEKOM, VAN, Gijsberdina Pieternella
IPC分类号： C07K14/395 , C12N1/36
CPC分类号： C12P7/10 , C07K14/395 , C12N1/36 , C12N9/0004 , C12N9/0006 , C12N9/10 , C12N9/1205 , C12N9/16 , C12N9/2405 , C12N9/2411 , C12N9/2437 , C12N9/2468 , C12N9/248 , C12N9/50 , C12N9/88 , C12N9/90 , C12N9/92 , C12P5/026 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/40 , C12P7/42 , C12P7/44 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/56 , C12P13/005 , C12P13/08 , C12P13/12 , C12P13/20 , C12P13/227 , C12P17/10 , C12P35/00 , C12Y101/01 , C12Y207/01016 , C12Y501/03004 , C12Y503/01004 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The invention relates to a process for the production of cells which are capable of converting arabinose, comprising the following steps: a)Introducing into a host strain that cannot convert arabinose, the genes AraA, araB and araD, this cell is designated as constructed cell; b)Subjecting the constructed cell to adaptive evolution until a cell that converts arabinose is obtained, c)Optionally, subjecting the first arabinose converting cell to adaptive evolution to improve the arabinose conversion; the cell produced in step b) or c) is designated as first arabinose converting cell; d)Analysing the full genome or part of the genome of the first arabinose converting cell and that of the constructed cell; e)Identifying single nucleotide polymorphisms (SNP's) in the first arabinose converting cell; and f)Using the information of the SNP's in rational design of a cell capable of converting arabinose; g)Construction of the cell capable of converting arabinose designed in step f).
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