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公开(公告)号：EP2459728A1
公开(公告)日：2012-06-06
申请号：EP10742423.6
申请日：2010-07-23
IPC分类号： C12P19/32
CPC分类号： C07H19/207 , C12P19/32
摘要： The invention concerns the enzymatic synthesis of stable analogues of nicotinamide adenine dinucleotide NAD/NADH and nicotinamide adenine dinucleotide phosphate NADP/NADPH, the so-called "carba-NADs", i.e. analogues of NAD/NADH or NADP/NADPH, respectively, comprising a carbacyclic sugar instead of ribose.
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公开(公告)号：EP2467478B1
公开(公告)日：2015-09-16
申请号：EP10757053.3
申请日：2010-08-18
发明人： RÖDEL, Wolfgang , HORN, Carina , STEINKE, Nelli , BUCCI, Nadine , MEIER, Thomas , SCHMUCK, Rainer , NAGEL, Rolf , HEINDL, Dieter
IPC分类号： C12N9/04
CPC分类号： C12N9/96 , C12N9/0006 , C12N9/0036 , C12Q1/26 , C12Q1/32 , C12Y101/01001 , C12Y101/01002 , C12Y101/01047 , C12Y101/01049 , C12Y106/05002 , C12Y106/99
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公开(公告)号：EP2459728B1
公开(公告)日：2013-06-05
申请号：EP10742423.6
申请日：2010-07-23
IPC分类号： C12P19/32
CPC分类号： C07H19/207 , C12P19/32
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公开(公告)号：EP2467478A1
公开(公告)日：2012-06-27
申请号：EP10757053.3
申请日：2010-08-18
发明人： RÖDEL, Wolfgang , HORN, Carina , STEINKE, Nelli , BUCCI, Nadine , MEIER, Thomas , SCHMUCK, Rainer , NAGEL, Rolf , HEINDL, Dieter
CPC分类号： C12N9/96 , C12N9/0006 , C12N9/0036 , C12Q1/26 , C12Q1/32 , C12Y101/01001 , C12Y101/01002 , C12Y101/01047 , C12Y101/01049 , C12Y106/05002 , C12Y106/99
摘要： The present invention relates to a method for stabilizing an enzyme by storing an enzyme in the presence of a stabilized coenzyme. The present invention further relates to an enzyme stabilized with a stabilized coenzyme and to the use thereof in test elements for detecting analytes.
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5.发明公开GENETICALLY ENGINEERED PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE COMPRISING AN AMINO ACID INSERTION 审中-公开有一组氨基基因改造PYRROLOQUINOLIN醌依赖性葡萄糖脱氢酶
公开(公告)号：EP1771553A1
公开(公告)日：2007-04-11
申请号：EP05769083.6
申请日：2005-07-19
发明人： BOENITZ-DULAT, Mara , LAGGERBAUER, Jessica , SCHMUCK, Rainer , KRATZSCH, Peter , KNAPPE, Wolfgang-Reinhold
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH) comprising an amino acid insertion between positions 428 and 429 as corresponding to the amino acid sequence known from Acinetobacter calcoaceticus, to genes encoding such variant sGDH, to proteins of such s-GDH variants with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugars, especially of glucose in a sample.
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6.发明授权VARIANTS OF SOLUBLE PYRROLOQUINOLINE QUINONE-DEPENDENT GLUCOSE DEHYDROGENASE 有权可溶性PYRROLOQUINOLIN醌依赖性葡萄糖脱氢酶的变异研究
公开(公告)号：EP1332216B1
公开(公告)日：2011-08-17
申请号：EP01988775.1
申请日：2001-10-20
发明人： KRATZSCH, Peter , SCHMUCK, Rainer , BUNK, Daniela , SHAO, Zhixin , THYM, Detlef , KNAPPE, Wolfgang-Reinhold
CPC分类号： C12N9/0006
摘要： The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.
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7.发明公开A WALK-THROUGH TECHNIQUE FOR i IN VITRO /i RECOMBINATION OF POLYNUCLEOTIDE SEQUENCES 审中-公开A“步行通过”以科技求多核苷酸的体外重组
公开(公告)号：EP1404825A2
公开(公告)日：2004-04-07
申请号：EP02747439.4
申请日：2002-06-26
IPC分类号： C12N9/16
CPC分类号： C12N9/0034 , C12N9/16 , C12N15/102
摘要： A simple and efficient method for random in vitro mutagenesis and recombination of polynucleotide sequences based on walk-through with chain-elongating molecules and chain terminating molecules e.g. dNTPs/ddNTPs followed by reassembly and amplification is described. The utility of this method for improving protein structure and/or function is demonstrated by creating novel sarcosine oxidase variants and by recombining human placental alkaline phosphates and calf intestinal alkaline phosphatase for better mutants with improved properties.
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8.发明授权THERMOSTABLE MUTANTS OF PYRROLOQUINOLINE QUINONE DEPENDENT GLUCOSE DEHYDROGENASE 有权THERMO稳定的突变体吡咯CHINON依赖葡萄糖脱氢酶
公开(公告)号：EP1802748B1
公开(公告)日：2011-06-01
申请号：EP05792997.8
申请日：2005-10-14
CPC分类号： C12N9/0006
摘要： The present invention relates to a mutant protein of PQQ-dependent s-GDH characterized in that in at least one of the positions 122 and 124 the amino acid lysine is present, wherein these positions correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence (SEQ ID NO: 2), it also discloses genes encoding such mutant s-GDH, and different applications of these sGDH mutants, particularly for determining the concentration of glucose in a sample.
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公开(公告)号：EP1360284B1
公开(公告)日：2008-07-16
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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公开(公告)号：EP1360284A2
公开(公告)日：2003-11-12
申请号：EP02750504.9
申请日：2002-02-08
发明人： MUELLER, Rainer , THALHOFER, Johann-Peter , REXER, Bernhard , SCHMUCK, Rainer , GEIPEL, Frank , GLASER, Stephan , SCHOEN, Helmut , MEIER, Thomas , RUDOLPH, Rainer , LILIE, Hauke , SCHOTT, Bjoern
CPC分类号： C12N9/58 , C07K2319/00 , C12Y304/21064
摘要： The invention relates to recombinant proteinase K and to a method for producing recombinant proteinase K, which is characterised by the following steps: a) transformation of a host cell containing a recombinant nucleic acid that codes for the zymogenic precursor of the proteinase K, b) cultivation of the host cell in such a way that the zymogenic precursor of proteinase K occurs in the form of inclusion bodies in said host cell, c) isolation of the inclusion bodies and re-naturing under conditions, from which the protease part of the zymogenic precursor emerges in its natural conformation, d) activation and purification of the re-natured proteinase K.
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