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公开(公告)号：EP2305704A4
公开(公告)日：2012-11-28
申请号：EP09800190
申请日：2009-07-15
IPC分类号： C07K14/375 , C07K14/37 , C12P21/02 , G01N33/53 , G01N33/574
CPC分类号： G01N33/574 , C07K14/37 , C12P21/02 , G01N2333/4724 , Y10T436/143333
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2.发明公开
公开(公告)号：EP1965211A4
公开(公告)日：2009-08-12
申请号：EP06834673
申请日：2006-12-14
CPC分类号： C12Q1/48 , C08B37/00 , G01N33/53 , G01N2333/4724 , G01N2333/91091 , G01N2400/00
摘要： It is intended to provide a method by which a sugar chain having GlcNAc transferred by GnT-V can be accurately detected, screened and purified. In this detection method, two kinds of lectins differing in detailed GlcNAc-specificity are used together. As shown in Fig. 1(I), for example, a sample containing a sugar chain having GlcNAc transferred by GnT-V is screened by using a lectin GSL-II, which recognizes a sugar chain having GlcNAc transferred by GnT-V, and then the screened sample is screened again by using another lectin BLL, which does not recognizes a sugar chain having GlcNAc transferred by GnT-V, to thereby detect, screen and purify the sugar chain having GlcNAc transferred by GnT-V alone.
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公开(公告)号：EP2405269A4
公开(公告)日：2012-08-15
申请号：EP10748464
申请日：2010-02-23
发明人： KUNO ATSUSHI , HIRABAYASHI JUN , MATSUDA ATSUSHI , NARIMATSU HISASHI , IKEHARA YUZURU , SHODA JUNICHI , KAWAMOTO TORU
IPC分类号： G01N33/574 , G01N21/64 , G01N37/00
CPC分类号： G01N21/648 , G01N33/57407 , G01N33/57438 , G01N33/57446
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公开(公告)号：EP2957630A4
公开(公告)日：2016-08-17
申请号：EP14751402
申请日：2014-02-13
发明人： TATENO HIROAKI , ITO YUZURU , ONUMA YASUKO , HIRABAYASHI JUN , ASASHIMA MAKOTO
CPC分类号： C12N5/0081 , C07K14/195 , C07K2319/33 , C07K2319/55 , C12N9/1077 , C12Y204/02036
摘要： An object of the present invention is to provide a method for introducing a target substance into undifferentiated cells, and a carrier therefor, whereby the target substance can be specifically introduced into the undifferentiated cells by contacting the undifferentiated cells with an rBC2LCN-target substance fusion product in which rBC2LCN lectin is fused to the target substance. Particularly, an rBC2LCN-toxin fusion product in which rBC2LCN lectin is fused to a toxin functioning in cells or its domain having the ability to kill cells functions as an agent for eliminating undifferentiated stem cells and can be administered into a medium after inducing the differentiation of the stem cells to reliably kill only the stem cells in an undifferentiated state.
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5.发明公开METHOD FOR ENRICHMENT AND SEPARATION OF SPINAL FLUID GLYCOPROTEIN, METHOD FOR SEARCHING FOR MARKER FOR CENTRAL NERVOUS SYSTEM DISEASES WHICH UTILIZES THE AFOREMENTIONED METHOD, AND MARKER FOR CENTRAL NERVOUS SYSTEM DISEASES 有权法富集分离RÜCKENMARKSFLÜSSIGKEITSGLYKOPROTEIN的，方法用于搜索标志物使用方法为中央神经系统疾病和标记为中央神经系统疾病
公开(公告)号：EP2653476A4
公开(公告)日：2015-10-14
申请号：EP11847931
申请日：2011-12-16
发明人： NARIMATSU HISASHI , HIRABAYASHI JUN , KUNO ATSUSHI , MATSUZAKI HIDEKI , IKEHARA YUZURU , ITO HIROMI , HASHIMOTO YASUHIRO , SHIROTANI KEIRO , FUTAKAWA SATOSHI , ARAI HAJIME , MIYAJIMA MASAKAZU , FUJIHARA KAZUO
CPC分类号： G01N33/6896 , G01N33/6842 , G01N2333/47 , G01N2333/4728 , G01N2400/40 , G01N2800/285 , H01J49/0036
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公开(公告)号：EP1710581A4
公开(公告)日：2007-10-10
申请号：EP04807690
申请日：2004-12-24
IPC分类号： G01N33/53 , G01N33/543 , G01N37/00
CPC分类号： G01N33/54393 , G01N33/543 , Y10S435/97
摘要： As a result of study of optimum conditions for the method of immobilizing a protein exhibiting an interaction with sugar chains on a substrate, it has been found that immobilization at an S/N ratio higher than in the prior art can be accomplished by applying a GTMS coat onto a surface of slide glass. Further, detection of any weak interaction between sugar chain and lectin with high sensitivity has been successfully accomplished by employing a substrate furnished with multiple reaction vessels lined with rubber having multiple holes and by carrying out lectin spotting and PBST washing. Moreover, through introduction of an evanescent excitation type scanner, it has become feasible to detect any interaction between lectin and sugar chain without the need to conduct washing removing operation for probe solution.
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7.发明公开CELL DIFFERENTIATION ASSAY METHOD, CELL ISOLATION METHOD, METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, AND METHOD FOR PRODUCING DIFFERENTIATED CELLS 审中-公开细胞分化测试程序方法隔离牢房，方法生成诱导的多能干细胞及其制造方法的分化细胞
公开(公告)号：EP2821498A4
公开(公告)日：2015-12-09
申请号：EP13755221
申请日：2013-02-27
发明人： TATENO HIROAKI , HIRABAYASHI JUN , ASASHIMA MAKOTO , ITO YUZURU , ONUMA YASUKO , WARASHINA MASAKI , FUKUDA MASAKAZU
IPC分类号： C12Q1/02 , C12M1/00 , C12M1/34 , C12N5/10 , G01N33/569
CPC分类号： G01N33/56966
摘要： Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fuc±1-2Gal²1-3GlcNAc" and/or "Fuc±1-2Gal²1-3GalNAc;" and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of "Fuc±1-2Gal²1-3GlcNAc" and/or "Fuc±1-2Gal²1-3GalNAc."
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8.发明公开UNDIFFERENTIATED CELL DETECTION METHOD AND COMPLEX CARBOHYDRATE DETECTION METHOD 有权未分化的细胞检测方法和复合碳水化合物检测方法
公开(公告)号：EP2774994A4
公开(公告)日：2015-09-02
申请号：EP12845274
申请日：2012-10-31
发明人： TATENO HIROAKI , HIRABAYASHI JUN , ITO YUZURU , ONUMA YASUKO , ASASHIMA MAKOTO , KUNO ATSUSHI , WARASHINA MASAKI , FUKUDA MASAKAZU
CPC分类号： G01N33/56966 , C07K14/195 , G01N2333/4724 , G01N2400/00
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9.发明公开SUGAR CHAIN MARKER AS MEASURE OF DISEASE CONDITIONS OF HEPATIC DISEASES 审中-公开ZUCKERKETTENMARKER ALS MASSSTABFÜRDIE KRANKHEITSSTADIEN VON LEBERERKRANKUNGEN
公开(公告)号：EP2455389A4
公开(公告)日：2013-06-19
申请号：EP10799822
申请日：2010-07-12
发明人： NARIMATSU HISASHI , HIRABAYASHI JUN , IKEHARA YUZURU , ANGATA TAKASHI , KAJI HIROYUKI , KUNO ATSUSHI , OHKURA TAKASHI , SHIKANAI TOSHIHIDE , SOGABE MAKI , TOGAYACHI AKIRA , OCHOU MAKOTO , TANAKA YASUHITO , MIZOKAMI MASASHI
CPC分类号： C07K9/00 , C07K14/47 , G01N33/57438 , G01N33/57469 , G01N2400/02 , G01N2800/085
摘要： The present invention is directed to developing a glycan markers capable of detecting a hepatic disease, and more specifically to developing a glycan marker indicating a hepatic disease-state. Furthermore, the present invention is also directed to developing a glycan marker capable of distinguishing hepatic disease-states with the progress of hepatocarcinoma. The present inventors identified, among the serum glycoproteins, glycopeptides and glycoproteins in which a glycan structure specifically changes due to a hepatic diseases including hepatocarcinoma and provide these as novel glycan markers (glycopeptide and glycoprotein) specific to hepatic disease-states.
摘要翻译： 本发明涉及开发能够检测肝脏疾病的聚糖标记物，更具体地说，涉及发展表达肝病状态的聚糖标记物。 此外，本发明还涉及开发能够区分肝脏疾病状态与肝癌进展的聚糖标记物。 本发明人鉴定了由于肝脏疾病（包括肝癌）而导致的聚糖结构特异性变化的糖蛋白和糖蛋白，并将其作为肝细胞特异性的新型聚糖标记（糖肽和糖蛋白）而鉴定出来。
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