公开(公告)号：EP3191605A1

公开(公告)日：2017-07-19

申请号：EP15767655.2

申请日：2015-09-09

申请人： The Broad Institute, Inc. ,  President and Fellows of Harvard College ,  Massachusetts Institute of Technology

发明人： REGEV, Aviv ,  MACOSKO, Evan, Zane ,  MCCARROLL, Steven, Andrew ,  SHALEK, Alexander, K. ,  BASU, Anindita ,  FORD, Christopher, B. ,  PARK, Hongkun ,  WEITZ, David, A.

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6809 ,  C12N15/1096 ,  C12Q1/6834 ,  C12Q1/6869 ,  G06K19/06 ,  C12Q2521/107 ,  C12Q2525/15 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/629 ,  C12Q2563/179

摘要： The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.

摘要翻译： 本发明总体上涉及分子条形码和基于乳液的微流体的组合，以高通量方式分离，裂解，条形码并从各个细胞制备核酸。

公开(公告)号：EP3368221A1

公开(公告)日：2018-09-05

申请号：EP16861016.0

申请日：2016-10-28

申请人： The Broad Institute Inc. ,  Massachusetts Institute Of Technology ,  President and Fellows of Harvard College ,  Dana-Farber Cancer Institute, Inc.

发明人： FORD, Christopher B. ,  WEITZ, David, A. ,  BASU, Anindita ,  REGEV, Aviv ,  ROTEM, Asaf ,  STRUHL, Kevin

IPC分类号： B01L3/00 ,  C12Q1/00

CPC分类号： B01L3/502715 ,  B01L3/0241 ,  B01L3/502746 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/08 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/086 ,  C12M23/12 ,  C12M23/16 ,  G01N33/5011 ,  G01N33/502

摘要： The present invention generally relates to a controlled fluidic device to develop spatially complex environments to enhance the rate of evolution in cell populations. The method further provides an enhanced understanding in the emergence, for example, drug resistance during cancer chemotherapy.

公开(公告)号：EP3132072A1

公开(公告)日：2017-02-22

申请号：EP15780044.2

申请日：2015-04-17

申请人： President and Fellows of Harvard College ,  The Broad Institute, Inc. ,  The General Hospital Corporation d/b/a Massachusetts General Hospital

发明人： BERNSTEIN, Bradley, E. ,  NICOL, Robert ,  WEITZ, David, A.

IPC分类号： C40B20/00 ,  C40B20/04 ,  C40B40/06 ,  C12P19/34 ,  G01N35/02

CPC分类号： B01J19/0046 ,  B01J2219/00599 ,  B01J2219/00722 ,  B01J2219/00743 ,  C12N15/10 ,  C12N15/1075 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q1/6874 ,  C40B50/10 ,  G01N15/10 ,  G01N2015/1081 ,  C12Q2521/501 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2563/185

摘要： The present invention generally relates to microfluidic devices, including systems and methods for tagging droplets within such devices. In some aspects, microfluidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid “tags,” and optionally ligating then nucleic acids together, the conditions that a droplet was exposed to may be encoded by the nucleic acid tags. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

摘要翻译： 本发明一般涉及微流体装置，包括用于在这些装置内标记液滴的系统和方法。 在一些方面，通过将液滴（或其他离散实体）暴露于各种不同的条件来操纵微流体液滴。 通过将多个核酸“标签”并入可选地将核酸连接在一起的液滴中，可以由核酸标签编码液滴暴露的条件。 因此，即使暴露于不同条件的液滴混合在一起，每个液滴遇到的条件仍然可以通过例如测序核酸来确定。

公开(公告)号：EP4352212A1

公开(公告)日：2024-04-17

申请号：EP22820931.8

申请日：2022-06-08

申请人： President and Fellows of Harvard College ,  Massachusetts Institute of Technology

发明人： INGBER, Donald, E. ,  GNYAWALI, Vaskar ,  HARRIER, Danielle ,  LIU, Yan ,  ZHAO, Evan, Minghao ,  WEITZ, David, A. ,  COLLINS, James, J. ,  SESAY, Adama, Marie ,  HANEY, Bobby, Tyrell

IPC分类号： C12N9/04 ,  A23L33/125 ,  A23L29/30 ,  A61K31/05

CPC分类号： A23L29/30 ,  A23L33/125 ,  A23L29/06 ,  C12N9/99 ,  C12Y101/03004 ,  C12N9/0006 ,  C12Y101/99011 ,  C12N9/1051 ,  C12Y204/01009 ,  C12Y204/0101 ,  C12N11/12 ,  C12N11/06

公开(公告)号：EP2307560A2

公开(公告)日：2011-04-13

申请号：EP09770562.8

申请日：2009-06-26

申请人： Massachusetts Institute of Technology ,  President and Fellows of Harvard College

发明人： WANG, Benjamin, L. ,  HUMPHRY, Katherine, J. ,  WEITZ, David, A. ,  STEPHANOPOULOS, Gregory

IPC分类号： C12Q1/04 ,  G01N21/00

CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/087 ,  B01L2400/0421

摘要： The present invention relates generally to the use of droplets to culture and/or assay cells or other species. In some cases, the cells or other species may be sorted based upon the results of the culture and/or assay. In some embodiments, cells other species can be encapsulated in droplets and exposed to one or more agents (e.g., a sugar, an indicator dye, etc.). For instance, in some cases, exposure of cells to the agents may result in the production of metabolites or other compounds (e.g., amino acids, proteins, organic acids, etc.) which may be, for example, assayed or otherwise determined. In some embodiments, the reaction of an agent with cells and/or other species within a droplet may reveal a property of the cells or other species (e.g., sugar consumption, growth rate, ability to withstand exposure to the agent, etc.). As an example, cells that produce desired metabolites or exhibit certain properties may be separated from the other cells via sorting techniques. Other aspects of the invention relate to devices or kits for implementing such sorts, methods of promoting such techniques, or the like.

公开(公告)号：EP2760578B1

公开(公告)日：2020-08-26

申请号：EP12860342.0

申请日：2012-09-27

申请人： President and Fellows of Harvard College

发明人： SPERLING, Ralph, Alexander ,  ABATE, Adam, R. ,  WEITZ, David, A.

IPC分类号： B01J19/26 ,  B01J8/06 ,  B01J19/24 ,  B01F13/00 ,  B01L3/02 ,  B01F3/08 ,  B01L3/00

公开(公告)号：EP3442707A1

公开(公告)日：2019-02-20

申请号：EP17783188.0

申请日：2017-04-14

申请人： President and Fellows of Harvard College

发明人： WEITZ, David, A. ,  MUTAFOPULOS, Kiryakos, S. ,  HUFNAGEL, Thomas

IPC分类号： B01L3/00 ,  G01N1/28 ,  F17D1/08

公开(公告)号：EP3283629A1

公开(公告)日：2018-02-21

申请号：EP16780825.2

申请日：2016-04-15

申请人： President and Fellows of Harvard College

发明人： WEITZ, David, A. ,  ZHANG, Huidan ,  HEYMAN, John ,  KLEIN, Allon, Moshe

IPC分类号： C12N15/10 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12P19/34 ,  C12N15/1006 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/6869 ,  C12Q2600/156 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2523/319 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2563/179

摘要： The present invention generally relates to microfluidics and labeled nucleic acids. In one aspect, the present invention is generally directed to a method, wherein the method includes providing a plurality of droplets comprising particles, the particles comprising oligonucleotides, and attaching a nucleic acid sequence to the oligonucleotides. Certain embodiments are generally directed to systems and methods for splitting a droplet into two or more droplets. Certain embodiments are generally directed to systems and methods for sorting fluidic droplets in a liquid.

公开(公告)号：EP3134536A1

公开(公告)日：2017-03-01

申请号：EP15783629.7

申请日：2015-04-17

申请人： President and Fellows of Harvard College ,  Vilnius University

发明人： WEITZ, David, A. ,  KLEIN, Allon, Moshe ,  AKARTUNA, Ilke ,  MAZUTIS, Linas ,  KIRSCHNER, Marc, W.

IPC分类号： C12P19/34

摘要： The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include "barcodes" or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

摘要翻译： 本发明一般涉及微流体和标记的核酸。 例如，某些方面通常涉及用于在微流体液滴内标记核酸的系统和方法。 在一组实施方案中，核酸可以包括可用于将液滴中的核酸与另一个液滴中的核酸区分开的“条形码”或唯一序列，例如甚至在核酸汇集在一起​​之后。 在一些情况下，可以使用颗粒将独特的序列并入单独的液滴中并连接到液滴内的核酸（例如从裂解的细胞中释放）。 在一些情况下，条形码可以用于区分数十，数百甚至数千个核酸，例如由不同细胞或其他来源产生的核酸。

公开(公告)号：EP3132037A1

公开(公告)日：2017-02-22

申请号：EP15780364.4

申请日：2015-04-17

申请人： President and Fellows of Harvard College ,  The General Hospital Corporation d/b/a Massachusetts General Hospital

发明人： WEITZ, David, A. ,  IAFRATE, Anthony J. ,  ZHENG, Zongli ,  ZHUANG, Huidan

IPC分类号： C12N15/10

CPC分类号： C12N15/1065 ,  C12N15/10 ,  C12N15/1037 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

摘要： The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfluidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid "tags," and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

摘要翻译： 本发明一般涉及微流体装置，包括用于在这些装置内标记液滴的方法和系统。 在一些方面，通过将液滴（或其他离散实体）暴露于各种不同的条件来操纵微液滴。 通过在液滴中掺入多个核酸“标签”，并任选地扩增核酸，例如在液滴内，微滴暴露的条件可由核酸编码。 因此，即使暴露于不同条件的液滴混合在一起，每个液滴遇到的条件仍然可以通过例如测序核酸来确定。