公开(公告)号：EP2585171B1

公开(公告)日：2018-12-26

申请号：EP11772857.6

申请日：2011-05-06

Applicant: University Of Central Florida Research Foundation, Inc.

Inventor： HICKMAN, James ,  GUO, Xiufang

IPC: A61P21/00 ,  C12N5/079 ,  C12N5/077

CPC classification number: G01N33/5058 ,  C12N5/0697 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/105 ,  C12N2501/13 ,  C12N2501/385 ,  C12N2501/41 ,  C12N2501/58 ,  C12N2502/081 ,  C12N2502/1335 ,  C12N2533/20 ,  C12N2535/10 ,  G01N33/48728 ,  G01N33/6872 ,  G01N33/6887 ,  G01N2500/10

Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.

公开(公告)号：EP2502985A1

公开(公告)日：2012-09-26

申请号：EP10831541.7

申请日：2010-11-16

Applicant: National University Corporation Okayama University

Inventor： YAMASHIRO, Takashi ,  KUROSAKA, Hiroshi ,  KAWANABE, Noriaki

IPC: C12N5/07 ,  A61K33/14 ,  A61K33/40 ,  A61K38/16 ,  A61K45/00 ,  A61P1/02 ,  A61P43/00 ,  C12N5/077

CPC classification number: A61K31/00 ,  A61K33/14 ,  A61K33/40 ,  A61K36/258 ,  A61K38/1709 ,  C12N5/0654 ,  C12N5/0664 ,  C12N2500/05 ,  C12N2501/00 ,  C12N2501/415 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  A61K2300/00

Abstract: Provided is an efficient method of inducing differentiation of a pulp cell into an odontoblast. Also provided is an agent for inducing differentiation capable of inducing differentiation into an odontoblast efficiently. The method of inducing differentiation of a pulp cell into an odontoblast includes using a substance capable of activating a Wnt signaling pathway. Further, the agent for inducing differentiation includes a substance capable of activating a Wnt signaling pathway. Specifically, the substance capable of activating a Wnt signaling pathway is any one selected from sodium perchlorate, sodium perchlorate, lithium chloride, Norrin, and R-Spondin2.

Abstract translation: 提供了一种诱导牙髓细胞分化为成牙质细胞的有效方法。 还提供了用于诱导能够有效诱导成牙本质细胞分化的药剂。 诱导牙髓细胞分化成成牙质细胞的方法包括使用能够激活Wnt信号通路的物质。 此外，用于诱导分化的药剂包括能够激活Wnt信号通路的物质。 具体地说，能够激活Wnt信号传导途径的物质是选自高氯酸钠，高氯酸钠，氯化锂，Norrin和R-斯朋素2中的任何一种。

公开(公告)号：EP2352816A1

公开(公告)日：2011-08-10

申请号：EP09824313.2

申请日：2009-11-06

Applicant: Sunnybrook Health Sciences Centre

Inventor： ZUNIGA-PFLÜCKER, Juan, Carlos ,  AWONG, Geneve ,  LA MOTTE-MOHS, Ross

IPC: C12N5/0783 ,  A61K35/14 ,  A61P31/18 ,  A61P35/00 ,  A61P37/06 ,  C12N5/10

CPC classification number: C12N5/0647 ,  A61K35/17 ,  A61K2035/124 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0663 ,  C12N5/0669 ,  C12N2501/2302 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/42 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  C12N2502/99 ,  C12N2506/02 ,  C12N2506/11 ,  C12N2506/45 ,  G01N33/5005

Abstract: Human progenitor T cells that are able to successfully engraft a murine thymus and differentiate into mature human T and NK cells are described The human progenitor T cells have the phenotype CD34+CD7+CD1a−CD5− or CD34+CD7+CD1a−CD5+ and are derived from human hematopoietic stem cells, embryonic stem cells and induced pluripotent stem cells b\ coculture with cells expressing a Notch receptor ligand (OP9-DL1 or OP9-DL4) Such cells are useful in a variety of applications including immune reconstitution, the treatment of immunodeficiencies and as carriers for genes used in gene therapy.

Abstract translation: 描述了能够成功移植鼠胸腺并分化成成熟的人T和NK细胞的人祖细胞T细胞。 人祖细胞T细胞具有表型CD34 + CD7 + CD1a-CD5-或CD34 + CD7 + CD1a-CD5 +，并且通过与表达Notch受体的细胞共培养来源于人造血干细胞，胚胎干细胞和诱导的多能干细胞 配体（OP9-DL1或OP9-DL4）。 这样的细胞可用于各种应用，包括免疫重建，免疫缺陷的治疗和用于基因治疗的基因的载体。

公开(公告)号：EP2012813A2

公开(公告)日：2009-01-14

申请号：EP07747399.9

申请日：2007-04-20

Applicant: Academisch Ziekenhuis Leiden

Inventor： 'T HOEN, Peter Abraham Christiaan ,  STERRENBERG, Petronella Johanna Elisabeth ,  DEN DUNNEN, Johannes Theodorus ,  VAN OMMEN, Garrit-Jan Boudewijn

IPC: A61K38/18 ,  C12N5/06

CPC classification number: A61K38/185 ,  C12N5/0658 ,  C12N15/113 ,  C12N15/1136 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/346 ,  C12N2320/33 ,  C12N2501/155 ,  C12N2502/1335 ,  C12N2510/00 ,  C12N2310/3521

Abstract: The present invention provides means and methods for alleviating genetic disease. A genetic defect that has a phenotype in differentiated cells can lead to defects in precursor cells thereof. These so-called secondary defects contribute to the overall disease of the individual. In the present invention, genetic intervention with the aim to alleviate symptoms of genetic disease is directed toward the primary genetic defect in the differentiated cell and the secondary defect in the precursor cell.

Abstract translation: 本发明提供的手段和用于减轻遗传病的方法。 遗传缺陷确实已分化的细胞的表型可以导致在其前体细胞的缺陷。 这些所谓的二次缺陷有助于个体的整体疾病。 在本发明中，与目的是减轻遗传病的症状的遗传干预朝向在所述分化细胞的主要遗传缺陷，并在前体细胞的二次缺陷。

公开(公告)号：EP0852463B1

公开(公告)日：2008-09-03

申请号：EP96918037.1

申请日：1996-06-03

Applicant: CASE WESTERN RESERVE UNIVERSITY ,  Osiris Therapeutics, Inc.

Inventor： CAPLAN, Arnold, I. ,  YOUNG, Randell, G. ,  DENNIS, James, E.

IPC: A01N63/00 ,  A01N65/00 ,  A61K35/34 ,  A61K35/26 ,  A61K35/28 ,  C12N5/00

CPC classification number: C12N5/0663 ,  A61K35/34 ,  A61K2035/124 ,  C12N5/0658 ,  C12N5/0668 ,  C12N2501/06 ,  C12N2502/1335 ,  C12N2502/1358 ,  C12N2502/1388 ,  A61K2300/00

Abstract: Compositions of matter which comprise a mixture of isolated muscle precursor cells with either isolated mesenchymal stem cells or isolated periosteum cells, optionally further including a myoinductive agent, a method for inducing isolated human mesenchymal stem cells to differentiate into myogenic cells, a method for producing dystrophin-positive myogenic cells in a mammal, a method for effecting muscle regeneration, and a method for treating muscular dystrophy are disclosed.

公开(公告)号：EP1765981A1

公开(公告)日：2007-03-28

申请号：EP05749468.4

申请日：2005-06-10

Applicant: Bernard O'Brien Institute of Microsurgery

Inventor： BORTOLOTTO, Susan, Kate ,  MESSINA, Aurora ,  ABBERTON, Keren, Maree

IPC: C12N5/00 ,  C12N5/08 ,  A61K35/12 ,  A61K35/34

CPC classification number: C12N5/0653 ,  A61K35/34 ,  C12N5/0068 ,  C12N2502/1335 ,  C12N2533/90

Abstract: The present invention relates generally to a tissue preparation including tissue cells and extracts thereof useful for promoting or facilitating the growth, development and differentiation of cells and tissues. More particularly, the present invention provides muscle-derived material comprising intact or extracted extracellular matrix and/or cells as well as cytokines, growth factors and other components. The muscle preparations of the present invention resemble basement membrane and are derived from cellular-based material. The muscle preparation may be used in vitro or in vivo as inter alia, a cellular scaffold in various tissue engineering applications and in other cell culture systems for nurturing and enriching a range of cell types including, but not limited to, precursor and stem cells such as pre-adipogenic cells. The muscle preparation is also useful as a base for creams, such as in the cosmetic and topical therapeutic industries and as a matrix or additive in the food industry.

公开(公告)号：EP2012813B1

公开(公告)日：2017-11-29

申请号：EP07747399.9

申请日：2007-04-20

Applicant: Academisch Ziekenhuis Leiden

Inventor： 'T HOEN, Peter Abraham Christiaan ,  STERRENBERG, Petronella Johanna Elisabeth ,  DEN DUNNEN, Johannes Theodorus ,  VAN OMMEN, Garrit-Jan Boudewijn

IPC: A61K38/18

CPC classification number: A61K38/185 ,  C12N5/0658 ,  C12N15/113 ,  C12N15/1136 ,  C12N2310/11 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/346 ,  C12N2320/33 ,  C12N2501/155 ,  C12N2502/1335 ,  C12N2510/00 ,  C12N2310/3521

公开(公告)号：EP2844744A2

公开(公告)日：2015-03-11

申请号：EP13713247.8

申请日：2013-02-21

Applicant: Brainstem Biotec Ltd. ,  Henry Ford Health System

Inventor： BRODIE, Chaya ,  SLAVIN, Shimon

IPC: C12N5/079 ,  A61P25/00 ,  A61P25/16 ,  C12N15/113

CPC classification number: A61K35/30 ,  A61K35/28 ,  A61K35/50 ,  A61K35/51 ,  C12N5/0622 ,  C12N15/113 ,  C12N2310/141 ,  C12N2320/11 ,  C12N2330/10 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2501/135 ,  C12N2501/195 ,  C12N2501/41 ,  C12N2501/65 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  C12N2506/025 ,  C12N2506/1353 ,  C12N2506/1369 ,  C12N2506/1384 ,  C12N2506/1392 ,  C12N2510/00 ,  C12Q1/6876 ,  C12Q2600/178

Abstract: A method of generating a population of cells useful for treating a nerve disease or disorder in a subject, the method comprising up-regulating a level of at least one exogenous mi RNA in mesenchymal stem cells (MSCs) and/or down-regulating a level of at least one mi RNA using a polynucleotide agent that hybridizes to the mi RNA, thereby generating the population of cells useful for treating the nerve disease or disorder. Isolated populations of cells generated thereby and uses thereof are also provided.

公开(公告)号：EP2334804A4

公开(公告)日：2012-05-23

申请号：EP09819852

申请日：2009-10-07

Applicant: IPIERIAN INC

Inventor： ELIAS GUILLERMO MUNOZ ,  JAVAHERIAN ASHKAN ,  IRION STEFAN

IPC: C12Q1/02 ,  C12N5/071 ,  G01N33/15

CPC classification number: G01N33/5073 ,  C12N5/0619 ,  C12N5/0676 ,  C12N2500/38 ,  C12N2501/13 ,  C12N2501/385 ,  C12N2502/081 ,  C12N2502/1305 ,  C12N2502/1335 ,  C12N2502/45 ,  C12N2503/02 ,  G01N33/5058 ,  G01N33/507

公开(公告)号：EP2356220A1

公开(公告)日：2011-08-17

申请号：EP09826922.8

申请日：2009-11-16

Applicant: University Of Louisville Research Foundation, Inc.

Inventor： RATAJCZAK, Janina ,  ZUBA-SURMA, Ewa, K. ,  RATAJCZAK, Mariusz

IPC: C12N5/071

CPC classification number: A61K35/28 ,  A61K35/51 ,  A61K2035/124 ,  C12N5/0647 ,  C12N5/0665 ,  C12N2502/1335 ,  C12N2502/1394

Abstract: Methods for isolating a CD133+/CD45neg/GlyAneg subpopulation of umbilical cord blood cells are disclosed. In some embodiments, the methods include providing an initial population of umbilical cord blood cells; contacting the initial population of cells with a first antibody that is specific for CD133, a second antibody that is specific for CD45, and a third antibody that is specific for Glycophorin A (GIyA) under conditions sufficient to allow binding of each antibody to its target, if present, on each cell of the initial population of cells; and isolating a subpopulation of cells that are CD133+, CD45neg, and GlyAneg. Also provided are isolated populations of CD133+/GlyAneg/CD45neg stem cells isolated from cord blood, methods for repopulating cell types in subjects, methods for bone marrow transplantation, methods for inducing hematopoietic competency in CD133+/GlyAneg/CD45neg stem cells, and cell culture systems that include CD133+/GIyAneg/CD45neg stem cells.