公开(公告)号：EP2736357A4

公开(公告)日：2015-05-06

申请号：EP12817489

申请日：2012-07-26

申请人： UNIV MISSOURI

发明人： FORGACS GABOR ,  MARGA FRANCOISE ,  JAKAB KAROLY ROBERT

IPC分类号： A23L1/31 ,  A23L1/317 ,  C12N5/00 ,  C12N5/077

CPC分类号： A23L13/00 ,  A23L13/03 ,  C12M21/08 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2502/1347 ,  C12N2502/28 ,  C12N2506/1307 ,  C12N2533/76

摘要： Provided are engineered meat products formed as a plurality of at least partially fused layers, wherein each layer comprises at least partially fused multicellular bodies comprising non-human myocytes and wherein the engineered meat is comestible. Also provided are multicellular bodies comprising a plurality of non-human myocytes that are adhered and/or cohered to one another; wherein the multicellular bodies are arranged adjacently on a nutrient-permeable support substrate and maintained in culture to allow the multicellular bodies to at least partially fuse to form a substantially planar layer for use in formation of engineered meat. Further described herein are methods of forming engineered meat utilizing said layers.

摘要翻译： 提供了形成为多个至少部分熔融层的工程肉制品，其中每层包括至少部分融合的包含非人肌细胞的多细胞体，并且其中所述工程肉是可食用的。 还提供了包含彼此粘附和/或粘附的多个非人肌细胞的多细胞体; 其中所述多细胞体相邻地排列在营养可渗透的支撑基底上并保持在培养物中，以允许所述多细胞体至少部分地融合以形成用于形成工程肉的基本平坦的层。 这里进一步描述的是利用所述层形成工程肉的方法。

公开(公告)号：EP2804944A1

公开(公告)日：2014-11-26

申请号：EP13705262.7

申请日：2013-01-14

申请人： Yeda Research and Development Co. Ltd.

发明人： ZIPORI, Dov ,  SHOSHANI, Ofer

IPC分类号： C12N5/071

CPC分类号： C12N5/0696 ,  C12N5/0663 ,  C12N13/00 ,  C12N2500/02 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  C12N2506/1353 ,  C12N2510/00

摘要： The invention relates to induction of reprogramming of somatic cells, by methods which require mild growth conditions. Disclosed are methods of inducing dedifferentiation of mesenchymal stromal cell (MSC), by seeding or incubating mesenchymal stromal cells (MSCs) at low density, and without introduction or expression of exogenous genes in the cells.

公开(公告)号：EP2736357A2

公开(公告)日：2014-06-04

申请号：EP12817489.3

申请日：2012-07-26

申请人： The Curators Of The University Of Missouri

发明人： FORGACS, Gabor ,  MARGA, Francoise ,  JAKAB, Karoly, Robert

IPC分类号： A23L1/31 ,  A23L1/317

CPC分类号： A23L13/00 ,  A23L13/03 ,  C12M21/08 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2502/1347 ,  C12N2502/28 ,  C12N2506/1307 ,  C12N2533/76

摘要： Provided are engineered meat products formed as a plurality of at least partially fused layers, wherein each layer comprises at least partially fused multicellular bodies comprising non-human myocytes and wherein the engineered meat is comestible. Also provided are multicellular bodies comprising a plurality of non-human myocytes that are adhered and/or cohered to one another; wherein the multicellular bodies are arranged adjacently on a nutrient- permeable support substrate and maintained in culture to allow the multicellular bodies to at least partially fuse to form a substantially planar layer for use in formation of engineered meat. Further described herein are methods of forming engineered meat utilizing said layers.

公开(公告)号：EP2502985A4

公开(公告)日：2013-04-24

申请号：EP10831541

申请日：2010-11-16

申请人： UNIV OKAYAMA NAT UNIV CORP

发明人： YAMASHIRO TAKASHI ,  KUROSAKA HIROSHI ,  KAWANABE NORIAKI

IPC分类号： C12N5/07 ,  A61K33/14 ,  A61K33/40 ,  A61K36/258 ,  A61K38/16 ,  A61K38/17 ,  A61K45/00 ,  A61P1/02 ,  A61P43/00 ,  C12N5/077

CPC分类号： A61K31/00 ,  A61K33/14 ,  A61K33/40 ,  A61K36/258 ,  A61K38/1709 ,  C12N5/0654 ,  C12N5/0664 ,  C12N2500/05 ,  C12N2501/00 ,  C12N2501/415 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  A61K2300/00

公开(公告)号：EP1344057A2

公开(公告)日：2003-09-17

申请号：EP01998129.9

申请日：2001-11-12

申请人： CHILDREN'S MEDICAL CENTER CORPORATION

发明人： ATALA, Anthony

IPC分类号： G01N33/50 ,  C12N5/06

CPC分类号： G01N33/5011 ,  A61K35/12 ,  C12N5/0685 ,  C12N5/0688 ,  C12N2502/1347 ,  C12N2502/28 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2533/40 ,  G01N33/5008 ,  G01N33/5088 ,  G01N2800/52

摘要： Organ system microarrays are provided comprising a substrate including a plurality of locations. Each location comprises a scaffold for supporting the growth and/or proliferation of cells and at lest one cell type. The microarrays provide reagents for testing the biological effect of test compounds on the function of miniature organ systems. In a further aspect of the invention, the microarrays allow expression profiles of an individual or population of individuals to be determined and compared. The organ system microarrays can be used in parallel with small animal testing or in post-animal testing to further characterize lead compounds prior to entering clinical trials, thus creating a substantial cost saving through the early elimination of lead compounds not likely to work in vivo .

公开(公告)号：EP2970877B1

公开(公告)日：2018-07-25

申请号：EP14763675.7

申请日：2014-03-17

申请人： Wake Forest University Health Sciences

发明人： Bitar, Khalil

IPC分类号： C12N5/0793 ,  C12N5/079 ,  C12N5/071 ,  C12N5/077 ,  A61K35/30 ,  A61K35/34

CPC分类号： C12N5/0619 ,  A61K35/30 ,  A61K35/34 ,  C12N5/0622 ,  C12N2502/1347 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

摘要： Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets. The matrix compositions of the present invention can be used to tissue engineer transplantable innervated GI smooth muscle constructs to remedy aganglionic disorders.

公开(公告)号：EP2970877A4

公开(公告)日：2016-09-14

申请号：EP14763675

申请日：2014-03-17

申请人： UNIV WAKE FOREST HEALTH SCIENCES

发明人： BITAR KHALIL

IPC分类号： C12N5/0793 ,  C12N5/071 ,  C12N5/077 ,  C12N5/079

CPC分类号： C12N5/0619 ,  A61K35/30 ,  A61K35/34 ,  C12N5/0622 ,  C12N2502/1347 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

摘要： Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets. The matrix compositions of the present invention can be used to tissue engineer transplantable innervated GI smooth muscle constructs to remedy aganglionic disorders.

公开(公告)号：EP2505638A1

公开(公告)日：2012-10-03

申请号：EP11002544.2

申请日：2011-03-28

申请人： Technische Universität Berlin

发明人： Marx, Uwe ,  Lorenz, Alexandra ,  Hoffmann, Silke

IPC分类号： C12N5/00

CPC分类号： C12N5/0697 ,  C12N2502/081 ,  C12N2502/1347 ,  C12N2503/04 ,  G01N33/5058

摘要： The present invention relates to a method of assembling a biological switching circuit (1). In a preferred embodiment the invention comprises assembly of a tissue capable of producing a signal, an afferent signal transmitter, neurons which can act as a control centre (4), an efferent signal transmitter and a tissue which is able to respond to the neuronal signal. The invention also relates to a biological switching circuit (1) producible by the method of the invention and its use.

摘要翻译： 本发明涉及组装生物开关电路（1）的方法。 在优选实施例中，本发明包括能够产生信号的组织的装配，传入信号发射器，可充当控制中心的神经元（4），传出信号发射器和能够对神经信号作出响应的组织 。 本发明还涉及通过本发明的方法生产的生物开关电路（1）及其用途。

公开(公告)号：EP2502985A1

公开(公告)日：2012-09-26

申请号：EP10831541.7

申请日：2010-11-16

申请人： National University Corporation Okayama University

发明人： YAMASHIRO, Takashi ,  KUROSAKA, Hiroshi ,  KAWANABE, Noriaki

IPC分类号： C12N5/07 ,  A61K33/14 ,  A61K33/40 ,  A61K38/16 ,  A61K45/00 ,  A61P1/02 ,  A61P43/00 ,  C12N5/077

CPC分类号： A61K31/00 ,  A61K33/14 ,  A61K33/40 ,  A61K36/258 ,  A61K38/1709 ,  C12N5/0654 ,  C12N5/0664 ,  C12N2500/05 ,  C12N2501/00 ,  C12N2501/415 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  A61K2300/00

摘要： Provided is an efficient method of inducing differentiation of a pulp cell into an odontoblast. Also provided is an agent for inducing differentiation capable of inducing differentiation into an odontoblast efficiently. The method of inducing differentiation of a pulp cell into an odontoblast includes using a substance capable of activating a Wnt signaling pathway. Further, the agent for inducing differentiation includes a substance capable of activating a Wnt signaling pathway. Specifically, the substance capable of activating a Wnt signaling pathway is any one selected from sodium perchlorate, sodium perchlorate, lithium chloride, Norrin, and R-Spondin2.

摘要翻译： 提供了一种诱导牙髓细胞分化为成牙质细胞的有效方法。 还提供了用于诱导能够有效诱导成牙本质细胞分化的药剂。 诱导牙髓细胞分化成成牙质细胞的方法包括使用能够激活Wnt信号通路的物质。 此外，用于诱导分化的药剂包括能够激活Wnt信号通路的物质。 具体地说，能够激活Wnt信号传导途径的物质是选自高氯酸钠，高氯酸钠，氯化锂，Norrin和R-斯朋素2中的任何一种。

公开(公告)号：EP2352816A1

公开(公告)日：2011-08-10

申请号：EP09824313.2

申请日：2009-11-06

申请人： Sunnybrook Health Sciences Centre

发明人： ZUNIGA-PFLÜCKER, Juan, Carlos ,  AWONG, Geneve ,  LA MOTTE-MOHS, Ross

IPC分类号： C12N5/0783 ,  A61K35/14 ,  A61P31/18 ,  A61P35/00 ,  A61P37/06 ,  C12N5/10

CPC分类号： C12N5/0647 ,  A61K35/17 ,  A61K2035/124 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0663 ,  C12N5/0669 ,  C12N2501/2302 ,  C12N2501/2307 ,  C12N2501/2315 ,  C12N2501/42 ,  C12N2502/13 ,  C12N2502/1305 ,  C12N2502/1311 ,  C12N2502/1317 ,  C12N2502/1323 ,  C12N2502/1329 ,  C12N2502/1335 ,  C12N2502/1341 ,  C12N2502/1347 ,  C12N2502/1352 ,  C12N2502/1358 ,  C12N2502/1364 ,  C12N2502/137 ,  C12N2502/1376 ,  C12N2502/1382 ,  C12N2502/1388 ,  C12N2502/1394 ,  C12N2502/99 ,  C12N2506/02 ,  C12N2506/11 ,  C12N2506/45 ,  G01N33/5005

摘要： Human progenitor T cells that are able to successfully engraft a murine thymus and differentiate into mature human T and NK cells are described The human progenitor T cells have the phenotype CD34+CD7+CD1a−CD5− or CD34+CD7+CD1a−CD5+ and are derived from human hematopoietic stem cells, embryonic stem cells and induced pluripotent stem cells b\ coculture with cells expressing a Notch receptor ligand (OP9-DL1 or OP9-DL4) Such cells are useful in a variety of applications including immune reconstitution, the treatment of immunodeficiencies and as carriers for genes used in gene therapy.

摘要翻译： 描述了能够成功移植鼠胸腺并分化成成熟的人T和NK细胞的人祖细胞T细胞。 人祖细胞T细胞具有表型CD34 + CD7 + CD1a-CD5-或CD34 + CD7 + CD1a-CD5 +，并且通过与表达Notch受体的细胞共培养来源于人造血干细胞，胚胎干细胞和诱导的多能干细胞 配体（OP9-DL1或OP9-DL4）。 这样的细胞可用于各种应用，包括免疫重建，免疫缺陷的治疗和用于基因治疗的基因的载体。