公开(公告)号：US07580126B2

公开(公告)日：2009-08-25

申请号：US11268590

申请日：2005-11-08

申请人： David Tuschel

发明人： David Tuschel

IPC分类号： G01J3/44

CPC分类号： G01J3/2889 ,  G01J3/44 ,  G01N21/3563 ,  G01N21/3577 ,  G01N21/65

摘要： The present disclosure describes methods and apparatus to produce a streaming image of a sample during a time period when an attribute of the sample is changing. The streaming image can be viewed in such a manner so as to be able to follow a visible change in an attribute of the sample. The sample may be undergoing nucleation, aggregation, or chemical interaction. The present disclosure also describes methods and apparatus to determine a change in an attribute of a sample by detecting, analyzing, and comparing spectra of the sample taken at different times during the time period when the attribute of the sample is changing. The sample may be undergoing nucleation, aggregation, or chemical interaction.

摘要翻译： 本公开描述了在样本的属性改变的时间段期间产生样本的流图像的方法和装置。 可以以这样的方式来观看流图像，以便能够跟踪样本的属性的可见变化。 样品可能经历成核，聚集或化学相互作用。 本公开还描述了通过检测，分析和比较在样本的属性正在改变的时间段期间在不同时间拍摄的样本的光谱来确定样本的属性变化的方法和装置。 样品可能经历成核，聚集或化学相互作用。

公开(公告)号：US07283241B2

公开(公告)日：2007-10-16

申请号：US11045080

申请日：2005-01-31

申请人： Xinghua Wang ,  Thomas C. Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick J. Treado

发明人： Xinghua Wang ,  Thomas C. Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick J. Treado

IPC分类号： G01N21/25

CPC分类号： G01N21/65 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/59 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/656

摘要： The disclosure generally relates to a multimode imaging apparatus for simultaneously obtaining multiple wavelength-discriminative spectral images of a sample. In one embodiment, the apparatus includes an image selector having a rotator assembly, the rotator assembly housing a first plurality of optical components, the image selector adapted to receive a illuminating photons having a first wavelength and direct the illuminating photons to the sample, the image selector adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; a microscope turret housing a second plurality of components, the microscope turret adapted to receive illuminating photons having a second wavelength and direct the photons to the sample; the microscopic turret adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; wherein substantially all of the interacted photons are selectively directed one of a plurality of detection sources to form multiple wavelength discriminative spectral images of the sample simultaneously.

摘要翻译： 本公开总体上涉及用于同时获得样本的多个波长鉴别光谱图像的多模式成像装置。 在一个实施例中，该装置包括具有旋转器组件的图像选择器，旋转体组件容纳第一多个光学部件，图像选择器适于接收具有第一波长的照明光子并将照射光子引导到样本，图像 选择器，适于接收与样本相互作用的照明光子，并选择性地将所述相互作用的光子引导到多个检测源之一; 显微镜转台，其容纳第二多个部件，所述显微镜转台适于接收具有第二波长并且将所述光子引导到所述样品的照明光子; 适于接收与样品相互作用的照明光子并且选择性地将所述相互作用的光子引导到多个检测源中的一个的微观炮塔; 其中基本上所有相互作用的光子被选择性地指向多个检测源中的一个，以同时形成样品的多个波长鉴别光谱图像。

公开(公告)号：US20060281068A1

公开(公告)日：2006-12-14

申请号：US11269596

申请日：2005-11-09

申请人： John Maier ,  Shona Stewart ,  Patrick Treado

发明人： John Maier ,  Shona Stewart ,  Patrick Treado

IPC分类号： C12Q1/00 ,  C12Q1/70 ,  C12Q1/68 ,  G06F19/00 ,  G01J3/44

CPC分类号： G01N21/65 ,  G01N2021/656

摘要： Raman molecular imaging (RMI) is used to detect mammalian cells of a particular phenotype. For example the disclosure includes the use of RMI to differentiate between normal and diseased cells or tissues, e.g., cancer cells as well as in determining the grade of said cancer cells. In a preferred embodiment benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view. Also, RMI techniques may be combined with visual image data and validated with other detection methods to produce confirm the matter obtained by RMI.

摘要翻译： 拉曼分子成像（RMI）用于检测特定表型的哺乳动物细胞。 例如，本公开包括使用RMI来区分正常和患病细胞或组织，例如癌细胞，以及确定所述癌细胞的等级。 在一个优选实施方案中，可以区分膀胱和其他组织的良性和恶性病变，包括上皮组织如肺，前列腺，肾，乳腺和结肠以及非上皮组织，例如骨髓和脑。 可以将与细胞或组织的疾病状态相关的拉曼散射数据与视觉图像数据组合，以产生描绘细胞结构的放大视图和与视野中的各个细胞的疾病状态有关的信息的混合图像。 此外，RMI技术可以与可视图像数据组合，并用其他检测方法验证，以确定RMI获得的事项。

公开(公告)号：US20060192956A1

公开(公告)日：2006-08-31

申请号：US11257219

申请日：2005-10-24

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC分类号： G01J3/28

CPC分类号： G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

摘要： A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

摘要翻译： 提供使用近红外辐射显微镜的化学成像系统。 该系统包括使用近红外辐射波长的光和可见波长的光来照射样品的区域的照明源。 收集来自样品的照射区域的透射，反射，发射或散射的近红外波长辐射光的多个空间解析光谱，并从其中产生准直光束。 提供近红外成像光谱仪用于选择准直光束的近红外辐射图像。 滤波后的图像由检测器收集以进一步处理。 同时检测来自样品的照射区域的可见波长的光，提供样品的同时可见和近红外化学成像分析。 提供了三维近红外化学成像显微镜的两种有效手段。

公开(公告)号：US20060146315A1

公开(公告)日：2006-07-06

申请号：US11364180

申请日：2006-03-01

申请人： Patrick Treado

发明人： Patrick Treado

IPC分类号： G01J3/44 ,  G01N21/64 ,  G01N21/65

CPC分类号： G01N21/65 ,  G01J3/02 ,  G01J3/0224 ,  G01J3/0229 ,  G01J3/0256 ,  G01J3/0264 ,  G01J3/0272 ,  G01J3/0283 ,  G01J3/10 ,  G01J3/44 ,  G01J3/4406 ,  G01N21/6456 ,  G01N2021/6417 ,  G01N2021/6423 ,  G01N2021/6471 ,  G01N2201/0221 ,  G01N2201/0627 ,  G02F1/133371 ,  G02F1/1347

摘要： The disclosure generally relates to a method and apparatus for compact dispersive imaging spectrometer. More specifically, one embodiment of the disclosure relates to a portable system for obtaining a spatially accurate wavelength-resolved image of a sample having a first and a second spatial dimension. The portable system can include a photon emission source for sequentially illuminating a plurality of portions of said sample with a plurality of photons to produce photons scattered by the sample. The photon emission source can illuminate the sample along the first spatial dimension for each of plural predetermined positions of the second spatial dimension. The system may also include an optical lens for collecting the scattered photons to produce therefrom filtered photons, a dispersive spectrometer for determining a wavelength of ones of the filtered photons, a photon detector for receiving the filtered photons and obtaining therefrom plural spectra of said sample, and a processor for producing a two dimensional image of said sample from the plural spectra.

摘要翻译： 本发明一般涉及紧凑型分散成像光谱仪的方法和装置。 更具体地，本公开的一个实施例涉及用于获得具有第一和第二空间维度的样本的空间上准确的波长分辨图像的便携式系统。 便携式系统可以包括光子发射源，用于用多个光子顺序照射所述样品的多个部分，以产生由样品散射的光子。 光子发射源可以沿着第一空间维度对第二空间维度的多个预定位置中的每一个照射样本。 该系统还可以包括用于收集散射光子以产生滤光光子的光学透镜，用于确定滤光光子中波长的波长的色散光谱仪，用于接收经滤光的光子并由此获得所述样品的多个光谱的光子检测器， 以及用于从所述多个光谱产生所述样本的二维图像的处理器。

公开(公告)号：US20060049354A1

公开(公告)日：2006-03-09

申请号：US11257139

申请日：2005-10-24

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC分类号： G01J5/02

CPC分类号： G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

摘要： A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

公开(公告)号：US08112248B2

公开(公告)日：2012-02-07

申请号：US12017445

申请日：2008-01-22

申请人： Robert Schweitzer ,  Patrick J. Treado ,  Jason Neiss

发明人： Robert Schweitzer ,  Patrick J. Treado ,  Jason Neiss

IPC分类号： G06F17/18 ,  G01N31/00

CPC分类号： G06K9/00536 ,  G06F19/703 ,  G06F19/709 ,  H01J49/0036

摘要： A system and method to search spectral databases and to identify unknown materials from multiple spectroscopic data in the databases. The methodology may be substantially automated and is configurable to determine weights to be accorded to spectroscopic data from different spectroscopic data generating instruments for improved identification of unknown materials. Library spectra from known materials are divided into training and validation sets. Initial, instrument-specific weighting factors are determined using a weight grid or weight scale. The training and validation spectra are weighted with the weighting factors and indicator probabilities for various sets of “coarse” weighting factors are determined through an iterative process. The finally-selected set of coarse weighting factors is further “fine tuned” using a weight grid with finer values of weights. The instrument-specific finer weight values may be applied to test data sets (or spectra) of an unknown material as well as to the library spectra from corresponding spectroscopic instruments. Instrument-specific weights for each class of samples may also be computed for additional customization and accuracy.

摘要翻译： 一种用于搜索光谱数据库并从数据库中的多个光谱数据中识别未知物质的系统和方法。 该方法可以基本上是自动化的，并且可配置为确定要与来自不同光谱数据生成装置的光谱数据一致的权重，以改进未知材料的识别。 已知材料的谱图谱分为训练和验证集。 使用权重网格或权重量表确定初始的仪器特定加权因子。 训练和验证光谱通过加权因子加权，并且通过迭代过程确定各组“粗”加权因子的指标概率。 最终选择的粗加权系数集合使用具有更精确的权重值的权重网格进一步“精细调整”。 仪器特定的更精细的重量值可以应用于未知材料的测试数据集（或光谱）以及来自相应光谱仪的文库光谱。 也可以计算每类样品的仪器特定重量，以获得额外的定制和准确性。

公开(公告)号：US07286231B2

公开(公告)日：2007-10-23

申请号：US10879630

申请日：2004-06-30

申请人： John S. Maier ,  Jason H. Neiss ,  Shona Stewart

发明人： John S. Maier ,  Jason H. Neiss ,  Shona Stewart

IPC分类号： G01J3/51 ,  G01N21/65

CPC分类号： G01J3/28 ,  G01J3/0227 ,  G01J3/027 ,  G01J3/0286 ,  G01J3/44

摘要： In one embodiment the disclosure relates to a method and a system for determining the corrected wavelength of a photon scattered by a sample. The method includes the steps of determining a wavelength of a photon scattered from a sample exposed to illuminating photons and passed through a tunable filter and correcting the determined wavelength of the photon as a function of the temperature of the tunable filter and as a function of the bandpass set point of the tunable filter. The step of correcting the determined wavelength can further include determining an offset and adding the offset to the determined wavelength of the photon.

摘要翻译： 在一个实施例中，本公开涉及一种用于确定由样本散射的光子的校正波长的方法和系统。 该方法包括以下步骤：确定从暴露于照射光子的样品散射的光子的波长，并且通过可调谐滤光器并校正所确定的光子波长作为可调滤光器的温度的函数，并且作为 可调谐滤波器的带通设定点。 校正所确定的波长的步骤还可以包括确定偏移并将偏移量加到所确定的光子的波长上。

公开(公告)号：US20060091311A1

公开(公告)日：2006-05-04

申请号：US11257222

申请日：2005-10-24

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC分类号： G01J5/02

CPC分类号： G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

摘要： A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

公开(公告)号：US20060033026A1

公开(公告)日：2006-02-16

申请号：US11256889

申请日：2005-10-24

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC分类号： G01N21/35

CPC分类号： G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

摘要： A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.