公开(公告)号：US20240327904A1

公开(公告)日：2024-10-03

申请号：US18619911

申请日：2024-03-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Qiang ZHANG ,  Linda HINH

IPC: C12Q1/6851 ,  C12Q1/6806

CPC classification number: C12Q1/6851 ,  C12Q1/6806

Abstract: Compositions and methods for fixing cells or nuclei or extracellular vesicles with dithiobismaleimidoethane (DTME) and subsequent reverse transcription in the cells or nuclei or extracellular vesicles are provided.

公开(公告)号：US20180010175A1

公开(公告)日：2018-01-11

申请号：US15711215

申请日：2017-09-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6841 ,  C12P19/34 ,  C12Q1/682 ,  C12Q1/6844 ,  C12Q2545/114 ,  C12Q2600/158 ,  C12Q2521/119 ,  C12Q2525/307 ,  C12Q2531/125 ,  C12Q2537/1376 ,  C12Q2543/101

Abstract: Methods and compositions for in situ detection of circular RNA in a tissue sample are provided.

公开(公告)号：US20240318244A1

公开(公告)日：2024-09-26

申请号：US18605670

申请日：2024-03-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Jon PETERSEN

IPC: C12Q1/6869 ,  C12N15/10 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/48 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6855

CPC classification number: C12Q1/6869 ,  C12N15/1096 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/485 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6855 ,  G01N2333/90245 ,  G01N2333/91097 ,  G01N2333/9125 ,  G01N2333/978

Abstract: Methods and compositions for nucleotide sequencing are provided. In some embodiments, click chemistry is used to link barcoding oligonucleotides to DNA fragments comprising adapters introduced by a transposase.

公开(公告)号：US20180258455A1

公开(公告)日：2018-09-13

申请号：US15977254

申请日：2018-05-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song GONG ,  John SULLIVAN ,  Rongdian FU ,  Yan WANG ,  Evan H. BURSEY ,  Man CHENG

IPC: C12P19/34 ,  C12Q1/686 ,  C12Q1/6804 ,  C07K16/40 ,  C12N9/12

CPC classification number: C12P19/34 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2319/80 ,  C12N9/1252 ,  C12Q1/6804 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2527/125 ,  C12Q2521/101

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

公开(公告)号：US20240229130A9

公开(公告)日：2024-07-11

申请号：US18381400

申请日：2023-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Ronald LEBOFSKY

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/682

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.

公开(公告)号：US20210403989A1

公开(公告)日：2021-12-30

申请号：US17357618

申请日：2021-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Man CHENG

IPC: C12Q1/6834 ,  C12Q1/6869

Abstract: Barcoding composition and methods involving solid supports having sets of different oligonucleotides that can be decoded to the same identification sequence.

公开(公告)号：US20170314060A1

公开(公告)日：2017-11-02

申请号：US15581401

申请日：2017-04-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Xiangdong MENG ,  Jan ZUR MEGEDE

IPC: C12Q1/48

CPC classification number: C12Q1/485 ,  C12Q1/48 ,  G01N2333/91245 ,  G01N2333/9126 ,  G01N2333/9128

Abstract: Methods of determining polymerase fidelity are provided. In one embodiment, the method comprises filling a gapped plasmid with a polymerase to form a gap-filled plasmid, wherein the gap-filled plasmid comprises a gene encoding an protein that is functional or non-functional depending on the polymerase fidelity; forming a plurality of partitions from a solution comprising the gap-filled plasmid and a label for detecting the presence of the plasmid; detecting the presence of the gap-filled plasmid in one or more of the partitions; and determining the fidelity of the polymerase by determining a ratio of partitions containing the gene encoding a functional protein to partitions containing a gene encoding a non-functional protein.

公开(公告)号：US20230383332A1

公开(公告)日：2023-11-30

申请号：US18203424

申请日：2023-05-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald LEBOFSKY ,  Josh SHINOFF ,  Adam M. McCOY ,  Man CHENG

IPC: C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6806 ,  C12Q1/6869 ,  C12N1/06

Abstract: Methods of making and forming partitions in nanovials are provided.

公开(公告)号：US20160273027A1

公开(公告)日：2016-09-22

申请号：US15034548

申请日：2014-11-21

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Steven T. OKINO ,  Man CHENG

IPC: C12Q1/68

CPC classification number: C12Q1/6813 ,  C12Q1/683 ,  C12Q2537/16 ,  C12Q2563/159

Abstract: The present invention provides methods for determining whether two or more nucleic acid molecules or two or more regions of a nucleic acid molecule in a sample are in close proximity to each other due to direct or indirect physical interactions.

Abstract translation: 本发明提供了用于确定样品中核酸分子的两个或多个核苷酸分子或两个或更多个区域是否由于直接或间接物理相互作用而彼此靠近的方法。

公开(公告)号：US20240132953A1

公开(公告)日：2024-04-25

申请号：US18381400

申请日：2023-10-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Man CHENG ,  Ronald LEBOFSKY

IPC: C12Q1/6874 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/682

Abstract: Methods and compositions for generating sequencing reads by partition of origin. One can introduce a unique molecular identifier and bead-specific barcodes to target nucleic acid fragments and the combination of bead-specific barcode, UMI and fragment can be used to identify when multiple bead-specific barcodes originated in the same partition, allowing for improved deconvolution of partition-based sequence analysis.