公开(公告)号：US09983205B2

公开(公告)日：2018-05-29

申请号：US14689791

申请日：2015-04-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Young Joon Kim

IPC: B01L3/00 ,  G01N33/543 ,  C12Q1/68 ,  B01L7/00

CPC classification number: G01N33/54366 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2300/043 ,  B01L2300/047 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2400/0487 ,  B01L2400/049 ,  C12Q1/6834 ,  C12Q1/6876 ,  C12Q2565/629 ,  C12Q2600/156

Abstract: Provided herein are cartridges, devices, and methods for carrying out multistep assays on a microfluidic scale. A cartridge includes a block frame comprising a well, wherein the well comprises an outlet, at least one inlet, and a bottom surface; a plurality of containers, wherein each container is connected to the well via a microchannel leading to the at least one inlet; and an openable cover, which cover when closed is configured to enclose an assay surface and form a gap between (i) the assay surface and the bottom surface of the well or (ii) the assay surface and the cover. The gap can be formed by a spacer that extends from the bottom surface of the well or from the outer edge of the cover. Methods include flowing liquid into the gap and/or through an opening adjacent to the assay surface.

公开(公告)号：US10343165B2

公开(公告)日：2019-07-09

申请号：US15396175

申请日：2016-12-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amir Sadri ,  Kun Guo ,  Daniel Y. Chu ,  Nenad Kircanski ,  Paul J. Patt ,  Tal Rosenzweig

IPC: G01N1/18 ,  G01N1/00 ,  B01D21/00 ,  B01L3/00 ,  G01N15/14 ,  B01L3/02 ,  C12Q1/24 ,  G01N15/10

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

公开(公告)号：US09535000B2

公开(公告)日：2017-01-03

申请号：US14479132

申请日：2014-09-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Amir M. Sadri ,  Daniel Y. Chu ,  Nenad Kircanski ,  Paul J. Patt ,  Tal Rosenzweig

IPC: G01N33/00 ,  G01N1/18 ,  B01L3/00 ,  F04B19/00 ,  G01N15/14 ,  B01L3/02 ,  C12Q1/24

CPC classification number: G01N15/1459 ,  B01L3/0265 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/084 ,  C12Q1/24 ,  G01N15/1404 ,  G01N2015/1413 ,  G01N2015/149 ,  Y10T436/25 ,  Y10T436/25375

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.

Abstract translation: 用于微流体操纵，分配和/或分选颗粒例如细胞和/或珠粒的系统，包括装置和方法。

公开(公告)号：US09678923B2

公开(公告)日：2017-06-13

申请号：US13861143

申请日：2013-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Paul J Patt

IPC: G01F17/00 ,  G01N15/00 ,  G01N15/02 ,  G06F17/00 ,  B01L3/02 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14

CPC classification number: G06F17/00 ,  B01L3/0289 ,  B01L2200/0652 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2400/0439 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N15/02 ,  G01N15/0205 ,  G01N2015/1062 ,  G01N2015/1486

Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

公开(公告)号：US20130270287A1

公开(公告)日：2013-10-17

申请号：US13861143

申请日：2013-04-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kun Guo ,  Paul J. Patt

IPC: G06F17/00

CPC classification number: G06F17/00 ,  B01L3/0289 ,  B01L2200/0652 ,  B01L2200/0657 ,  B01L2200/143 ,  B01L2400/0439 ,  B01L2400/0478 ,  B01L2400/0481 ,  G01N15/02 ,  G01N15/0205 ,  G01N2015/1062 ,  G01N2015/1486

Abstract: Particles are dispensed under controlled conditions to achieve an accurate number of particles by passing a suspension of the particles through a particle detecting device, obtaining a cumulative particle count, comparing the cumulative particle count with a target value, and shutting off the suspension flow once the particle count reaches the target value, all performed by automated means.

Abstract translation: 在受控条件下分配颗粒以通过使颗粒的悬浮液通过颗粒检测装置获得精确数量的颗粒，获得累积颗粒数，将累积颗粒数与目标值进行比较，并一旦关闭悬浮液流 粒子数达到目标值，全部以自动方式进行。

公开(公告)号：US20170128941A1

公开(公告)日：2017-05-11

申请号：US15396175

申请日：2016-12-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amir Sadri ,  Kun Guo ,  Daniel Y. Chu ,  Nenad Kircanski ,  Paul J. Patt ,  Tal Rosenzweig

IPC: B01L3/00 ,  G01N15/14

CPC classification number: B01L3/502761 ,  B01L3/0265 ,  B01L3/502738 ,  B01L3/502776 ,  B01L2200/027 ,  B01L2200/0652 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2200/146 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/088 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/16 ,  B01L2300/165 ,  B01L2300/18 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0688 ,  B01L2400/082 ,  B01L2400/084 ,  C12Q1/24 ,  G01N15/1056 ,  G01N15/1404 ,  G01N15/1484 ,  G01N2015/1006 ,  G01N2015/1081 ,  G01N2015/1413 ,  G01N2015/149 ,  Y10T436/25 ,  Y10T436/25375

Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.