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    Microfluidic system with fluid pickups
    3.
    发明授权
    Microfluidic system with fluid pickups 有权

    公开(公告)号：US10682647B2

    公开(公告)日：2020-06-16

    申请号：US15633413

    申请日：2017-06-26

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Anthony J. Makarewicz, Jr. ,  Luc Bousse ,  Stefano Schiaffino

    IPC: G01N1/00 ,  B01L3/00 ,  B01L7/00 ,  C12N15/10 ,  C12Q1/6806

    Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.

    DROPLET GENERATION SYSTEM WITH FEATURES FOR SAMPLE POSITIONING
    6.
    发明申请
    DROPLET GENERATION SYSTEM WITH FEATURES FOR SAMPLE POSITIONING 审中-公开
    具有样品定位特征的DROPLET生成系统

    公开(公告)号：US20140024023A1

    公开(公告)日：2014-01-23

    申请号：US13949115

    申请日：2013-07-23

    Applicant: Bio-Rad Laboratories, Inc

    Inventor： Thomas H. Cauley, III ,  Timothy Brackbill ,  Luc Bousse ,  Jon Petersen

    IPC: G01N1/28

    CPC classification number: G01N1/28 ,  B01F3/0807 ,  B01F13/0062 ,  B01L3/502784 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/084 ,  Y10T436/25

    Abstract: System, including methods and apparatus, for forming droplets of an emulsion. The system may include a channel junction at which a stream of sample fluid is divided into droplets by a dividing flow of carrier fluid. The system also may include one or more features configured to position sample fluid for reduced contact between the sample fluid and one or more surface regions of the channel junction, which may improve the consistency of droplet formation. In exemplary embodiments, sample fluid may be positioned by a step member produced by an increase in channel depth, and/or by directing flow of carrier fluid to form a barrier layer between sample fluid and a wall region, such as a ceiling region or floor region, of a channel network.

    Abstract translation: 用于形成乳液液滴的系统，包括方法和装置。 该系统可以包括通道结，在该通道连接处，样品流体流通过载体流体的分流被分成液滴。 该系统还可以包括被配置成定位样品流体以减少样品流体和通道结的一个或多个表面区域之间的接触的一个或多个特征，这可以改善液滴形成的一致性。 在示例性实施例中，样品流体可以由通过增加通道深度产生的台阶构件定位，和/或通过引导载体流体的流动来形成样品流体和壁区域之间的阻挡层，例如天花板区域或地板 区域，通道网络。

    MICROFLUIDIC SYSTEM WITH FLUID PICKUPS
    7.
    发明申请
    MICROFLUIDIC SYSTEM WITH FLUID PICKUPS 审中-公开

    公开(公告)号：US20170291174A1

    公开(公告)日：2017-10-12

    申请号：US15633413

    申请日：2017-06-26

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Anthony J. Makarewicz, JR. ,  Luc Bousse ,  Stefano Schiaffino

    IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/68

    Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.

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