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公开(公告)号：US10961518B2
公开(公告)日：2021-03-30
申请号：US16248442
申请日：2019-01-15
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Xiangdong Meng , Man Cheng
Abstract: Mutant Type-A DNA polymerases having increased resistance to one or more polymerization activity inhibitors are provided.
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2.发明授权公开(公告)号：US10787694B2
公开(公告)日：2020-09-29
申请号：US15977254
申请日：2018-05-11
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
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公开(公告)号：US11479816B2
公开(公告)日：2022-10-25
申请号：US16545611
申请日：2019-08-20
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Ronald Lebofsky , Andrew Kohlway , Jennifer Chew , Zachary Burkett , Man Cheng
IPC: C12Q1/6869 , G16B40/10 , G16B30/10
Abstract: Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.
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4.发明授权公开(公告)号：US09994887B2
公开(公告)日：2018-06-12
申请号：US14482756
申请日：2014-09-10
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Xiao-Song Gong , John Sullivan , Rongdian Fu , Yan Wang , Evan H. Bursey , Man Cheng
CPC classification number: C12P19/34 , C07K16/40 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2319/80 , C12N9/1252 , C12Q1/6804 , C12Q1/686 , C12Q2531/113 , C12Q2527/125 , C12Q2521/101
Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.
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公开(公告)号：US09556423B2
公开(公告)日：2017-01-31
申请号：US14512924
申请日：2014-10-13
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Man Cheng
CPC classification number: C12N9/12 , C07K2319/21 , C07K2319/23 , C07K2319/41 , C07K2319/42 , C07K2319/80 , C12N9/1247 , C12N9/1252 , C12N9/127 , C12N9/1276 , C12Q1/6848 , C12Q1/686 , C12Q2521/101 , C12Q2522/101 , C12Q2527/119 , C12Q2527/125 , C12Q2527/137
Abstract: The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.
Abstract translation: 本发明提供了改善核酸扩增特异性的方法，包括用核酸分子与聚合酶-Sso7 DNA结合结构域缀合物和精氨酸，亚精胺或精胺孵育。 本发明还提供了用于提高核酸扩增特异性的反应混合物和试剂盒。
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Patent Agency Ranking
公开(公告)号：US20160145587A1
公开(公告)日：2016-05-26
申请号：US14817064
申请日：2015-08-03
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Man Cheng
CPC classification number: C12N9/1252 , C12N9/96 , C12Y207/07 , C12Y207/07007
Abstract: Liquid storage solutions comprising polymerases are provided.
Abstract translation: 提供了包含聚合酶的液体储存溶液。
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公开(公告)号：US10287620B2
公开(公告)日：2019-05-14
申请号：US15581401
申请日：2017-04-28
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Man Cheng , Xiangdong Meng , Jan Zur Megede
Abstract: Methods of determining polymerase fidelity are provided. In one embodiment, the method comprises filling a gapped plasmid with a polymerase to form a gap-filled plasmid, wherein the gap-filled plasmid comprises a gene encoding an protein that is functional or non-functional depending on the polymerase fidelity; forming a plurality of partitions from a solution comprising the gap-filled plasmid and a label for detecting the presence of the plasmid; detecting the presence of the gap-filled plasmid in one or more of the partitions; and determining the fidelity of the polymerase by determining a ratio of partitions containing the gene encoding a functional protein to partitions containing a gene encoding a non-functional protein.
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公开(公告)号：US20180305739A1
公开(公告)日：2018-10-25
申请号：US16009894
申请日：2018-06-15
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Man Cheng , Xiao-Song Gong , Yan Wang , Adam M. McCoy
IPC: C12Q1/6806 , C12Q1/686 , C12P19/34
CPC classification number: C12Q1/6806 , C12P19/34 , C12Q1/6853 , C12Q1/686 , C12Q2521/107 , C12Q2521/131 , C12Q2521/327 , C12Q2525/207 , C12Q2525/121
Abstract: Methods of detecting and amplifying short RNAs are provided.
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公开(公告)号：US10053676B2
公开(公告)日：2018-08-21
申请号：US14817064
申请日：2015-08-03
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Man Cheng
CPC classification number: C12N9/1252 , C12N9/96 , C12Y207/07 , C12Y207/07007
Abstract: Liquid storage solutions comprising polymerases are provided.
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公开(公告)号：US20150166968A1
公开(公告)日：2015-06-18
申请号：US14562396
申请日：2014-12-05
Applicant: Bio-Rad Laboratories, Inc.
CPC classification number: C12N9/1252 , C07K2319/80 , C12N9/22 , C12Q1/686
Abstract: Fusion polypeptides having a heterologous 5′-3′ exonuclease domain linked to a polymerase that does not naturally have 5′-3′ exonuclease activity, as well as methods of their use are provided. Other aspects are also disclosed.
Abstract translation: 提供了与天然不具有5'-3'核酸外切酶活性的聚合酶连接的具有异源5'-3'外切核酸酶结构域的融合多肽及其使用方法。 还公开了其他方面。
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