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公开(公告)号：US10415046B2
公开(公告)日：2019-09-17
申请号：US15338888
申请日：2016-10-31
Applicant: DOW AGROSCIENCES LLC
Inventor： W. Michael Ainley , James W. Bing , David R. Corbin , Steven L. Evans , Joseph F. Petolino , Lakshmi Sastry-Dent , Steven A. Thompson , Steven R. Webb , Mary E. Welter , Ning Zhou
Abstract: The present invention claims methods for the stable integration of exogenous DNA into a specific locus, E32, in the maize genome through the use of zinc finger nucleases. Maize plants and plant parts that were transformed by the methods of the invention are claimed. The invention is useful for creating desirable traits such as herbicide resistance, herbicide tolerance, insect resistance, insect tolerance, disease resistance, disease tolerance, stress tolerance, and stress resistance in maize The E32 locus represents a superior site for inserting foreign genes because native agronomic phenotypes are not disturbed.
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公开(公告)号：US20170137834A1
公开(公告)日：2017-05-18
申请号：US15331126
申请日：2016-10-21
Applicant: Dow AgroSciences LLC
Inventor： Manju Gupta , Sandeep Kumar , Navin Elango , Jeffrey Beringer , Shavell Gorman , Andrew F. Worden , Sara Bennett , Daren Hemingway , Wei Chen , Huixia Wu , Ning Zhou , Michelle Sprint Smith
IPC: C12N15/82 , C07K14/325
CPC classification number: C12N15/8227 , C12N9/14 , C12N15/8225 , C12N15/8286 , C12Y303/01001 , Y02A40/162
Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays GRMZM2G015295 gene regulatory elements.
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公开(公告)号：US20150337394A1
公开(公告)日：2015-11-26
申请号：US14410793
申请日：2013-06-25
Applicant: DOW AGROSCIENCES LLC
Inventor： Lauren Clark , Kelley Ann Smith , Yang Wang , Ning Zhou
IPC: C12Q1/68
CPC classification number: C12Q1/6895 , C12N15/8277 , C12N15/8286 , C12Q2600/13 , C12Q2600/156 , Y02A40/162
Abstract: Soybean Event pDAB9582.816.15.1 comprises gene expression cassettes which contain genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. The disclosure provides polynucleotiderelated event detection methods. The present disclosure relates to a method for detecting a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated Soybean Event pDAB9582.816.15,1. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome. SEQ ID NO: 1 and SEQ ID N0:2 are diagnostic for Soybean Event pDAB9582.816.15.1.
Abstract translation: 大豆事件pDAB9582.816.15.1包含基因表达盒，其含有编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及用于作物保护和保护储存产品的方法。 本公开提供了多核苷酸相关事件检测方法。 本公开涉及一种用于检测新的耐昆虫和除草剂耐受转基因大豆转化事件的方法，其命名为大豆事件pDAB9582.816.15,1。 含有该事件的大豆植物的DNA包括本文所述的连接/侧翼序列，其表征插入的DNA在大豆基因组内的位置。 SEQ ID NO：1和SEQ ID N0：2是大豆事件pDAB9582.816.15.1的诊断。
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4.发明授权公开(公告)号：US10323287B2
公开(公告)日：2019-06-18
申请号：US14989787
申请日：2016-01-06
Applicant: DOW AGROSCIENCES LLC
Inventor： Yunxing Cory Cui , Jill Bryan , Donald Maum , Greg Gilles , Terry Wright , Jennifer Hamilton , Nicole Arnold , Nathan VanOpdorp , Tina Kaiser , Ning Zhou
IPC: C12Q1/68 , C12N15/82 , C12Q1/6895 , A01H5/10 , C12N9/02
Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.
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公开(公告)号：US20170283865A1
公开(公告)日：2017-10-05
申请号：US15249684
申请日：2016-08-29
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew A. Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
CPC classification number: C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
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Patent Agency Ranking
6.发明申请INSECT RESISTANT AND HERBICIDE TOLERANT SOYBEAN EVENT pDAB9582.816.15.1 审中-公开耐药和除草剂耐受性大豆事件pDAB9582.816.15.1公开(公告)号：US20150335017A1
公开(公告)日：2015-11-26
申请号：US14410762
申请日：2013-06-25
Applicant: DOW AGROSCIENCES LLC
Inventor： Nathan Bard , Gregory A. Bradfisch , Yunxing Cory Cui , James E. Dripps , Thomas Hoffman , Dayakar Pareddy , Dawn M. Parkhurst , Sandra G. Toledo , Barry Wiggins , Ning Zhou , Aaron T. Woosley
CPC classification number: A01N43/16 , A01H5/10 , C12N15/8274 , C12N15/8286 , Y02A40/162
Abstract: Soybean event 9582.816.15.1 comprises genes encoding Cry1F, Cry1Ac (synpro), and PAT, affording insect resistance and herbicide tolerance to soybean crops containing the event, and enabling methods for crop protection and protection of stored products. Embodiments of the present disclosure relate to a new insect resistant and herbicide tolerant transgenic soybean transformation event, designated soybean event pDAB9582.816.15.1, comprising cry1F v3 (cry1F), cry1Ac synpro (cry1Ac) and pat v6 (pat), as described herein, inserted into a specific site within the genome of a soybean cell. Representative soybean seed has been deposited with American Type Culture Collection (ATCC) with the Accession No. ATCC Deposit No. PTA-12588. The DNA of soybean plants containing this event includes the junction/flanking sequences described herein that characterize the location of the inserted DNA within the soybean genome.
Abstract translation: 大豆事件9582.816.15.1包含编码Cry1F，Cry1Ac（synpro）和PAT的基因，为含有该事件的大豆作物提供抗昆虫抗性和除草剂耐受性，以及对作物保护和保护储存产品的有效方法。 本公开的实施方案涉及如本文所述的新的耐昆虫抗性和除草剂耐受转基因大豆转化事件，称为大豆事件pDAB9582.816.15.1，包含cry1F v3（cry1F），cry1Ac synpro（cry1Ac）和pat v6（pat） 插入大豆细胞基因组内的特定位点。 代表性的大豆种子已经存入具有登录号ATCC保藏号PTA-12588的美国典型培养物保藏中心（ATCC）。 含有该事件的大豆植物的DNA包括本文所述的连接/侧翼序列，其表征插入的DNA在大豆基因组内的位置。
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公开(公告)号：US20140298547A1
公开(公告)日：2014-10-02
申请号：US14105223
申请日：2013-12-13
Applicant: Dow AgroSciences LLC
Inventor： Lakshmi Sastry-Dent , Matthew Simpson , Zehui Cao , Wei Chen , Ning Zhou , Steven R. Webb
IPC: C12Q1/68
CPC classification number: C12Q1/6858 , A01G24/00 , C12N5/00 , C12N15/1082 , C12N15/66 , C12N15/70 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B40/08
Abstract: The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Abstract translation: 本公开提供了用于检测和鉴定含有精确靶向基因组基因座的植物事件的方法，以及包含这种靶基因组基因座的植物和植物细胞。 该方法可以部署为用于筛选靶基因组基因座的完整性或破坏的高通量过程，并且任选地用于检测目标基因组基因座上的供体DNA多核苷酸插入。 该方法容易适用于鉴定通过使用位点特异性核酸酶产生的靶向方法产生的植物事件。
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公开(公告)号：US11425906B2
公开(公告)日：2022-08-30
申请号：US17224694
申请日：2021-04-07
Applicant: Dow AgroSciences LLC , M.S. Technologies, L.L.C.
Inventor： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn M. Parkhurst , Sandra G. Toledo , Terry R. Wright , Sean M. Russell , Bruce Held , Vaithilingam Sekar
Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
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公开(公告)号：US20210267198A1
公开(公告)日：2021-09-02
申请号：US17224694
申请日：2021-04-07
Applicant: Dow AgroSciences LLC , M.S. Technologies, L.L.C.
Inventor： Yunxing C. Cui , Thomas Hoffman , Ning Zhou , Stephen N. Novak , Julissa Colon , Dawn M. Parkhurst , Sandra G. Toledo , Terry R. Wright , Sean M. Russell , Bruce Held , Vaithilingam Sekar
Abstract: This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.
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10.发明授权公开(公告)号：US11098322B2
公开(公告)日：2021-08-24
申请号：US16279055
申请日：2019-02-19
Applicant: DOW AGROSCIENCES LLC
Inventor： Yunxing Cory Cui , Jill Bryan , Donald Maum , Greg Gilles , Terry Wright , Jennifer Hamilton , Nicole Arnold , Nathan VanOpdorp , Tina Kaiser , Ning Zhou
IPC: C12N15/82 , C12Q1/6895 , C12N9/02
Abstract: This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (or corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided.
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