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公开(公告)号：US08361764B1
公开(公告)日：2013-01-29
申请号：US12893922
申请日：2010-09-29
CPC分类号： C12N9/18 , C12P7/42 , C12Y301/01073
摘要： Novel genes that code for a family of feruloyl esterases that break down ferulic acid crosslinks between polysaccharide chains and between polysaccharides and lignins in plant cell walls are described herein as well as a method of rapid gene discovery.
摘要翻译： 本文描述了编码一种阿魏酸酯酶家族的新基因，其中分离了多糖链之间的阿魏酸交联，以及在植物细胞壁中的多糖和木质素之间的交联，以及快速基因发现的方法。
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公开(公告)号：US06703219B1
公开(公告)日：2004-03-09
申请号：US09274454
申请日：1999-03-22
IPC分类号： C12P2106
CPC分类号： C07K14/4737 , A61K38/00
摘要： The invention provides a mutant protein which has the same amino acid sequence as an unmutated C-reactive protein (CRP) subunit or an unmutated preCRP, except that at least one amino acid of the unmutated CRP subunit or unmutated preCRP has been deleted, at least one amino acid of the unmutated CRP subunit or unmutated preCRP has been replaced by another amino acid, at least one amino acid has been added to the unmutated CRP subunit or preCRP, or a combination of such changes has been made. The amino acid(s) added, deleted and/or replaced are chosen so that the mutant protein is less likely to form covalently cross-linked aggregates than the unmutated CRP subunit or unmutated preCRP. The mutant protein also exhibits at least one of the biological activities of modified-CRP. The invention also provides DNA molecules coding for the mutant proteins of the invention, vectors for expressing the mutant proteins, host cells which have been transformed so that they can express the mutant proteins, and a method of producing the mutant proteins of the invention comprising culturing the transformed host cells. Finally, the invention provides methods and materials for using the mutant proteins.
摘要翻译： 本发明提供了与未突变的C-反应蛋白（CRP）亚基或未突变的preCRP具有相同氨基酸序列的突变蛋白，除了未突变的CRP亚基或未突变的preCRP的至少一个氨基酸已被缺失，至少 未突变的CRP亚基或未突变的preCRP的一个氨基酸已经被另一个氨基酸替代，至少一个氨基酸已经添加到未突变的CRP亚基或preCRP中，或者已经进行了这些改变的组合。 选择加入，缺失和/或替换的氨基酸，使得突变蛋白不比未突变的CRP亚基或未突变的preCRP形成共价交联的聚集体。 突变蛋白还表现出修饰CRP的至少一种生物活性。 本发明还提供编码本发明的突变型蛋白质的DNA分子，用于表达突变型蛋白质的载体，已被转化的宿主细胞，使得它们能够表达突变型蛋白质，以及产生本发明的突变型蛋白质的方法，包括培养 转化的宿主细胞。 最后，本发明提供了使用突变蛋白的方法和材料。
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公开(公告)号：US20160060663A1
公开(公告)日：2016-03-03
申请号：US14843525
申请日：2015-09-02
申请人： Katrin GRAMMANN , Jan Wolter , Liv Reinecke , Steffen Schaffer , Eileen C. Spindler , Wendy K. Ribble , Brittany L. Prather , Catherine B. Poor , Tanya Warnecke Lipscomb , Hans H. Liao , Dave A. Hogsett , Ronald J. Evans
发明人： Katrin GRAMMANN , Jan Wolter , Liv Reinecke , Steffen Schaffer , Eileen C. Spindler , Wendy K. Ribble , Brittany L. Prather , Catherine B. Poor , Tanya Warnecke Lipscomb , Hans H. Liao , Dave A. Hogsett , Ronald J. Evans
CPC分类号： C12P7/6436 , C12N9/1029 , C12Y203/01041 , C12Y203/01075
摘要： A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester.
摘要翻译： 微生物细胞用于生产至少一种脂肪酸酯，其中所述细胞被遗传修饰以包含（i）至少一种能使细胞产生至少一种脂肪酸和/或酰基辅酶A（CoA）的第一遗传突变 ），其相对于丙二酰辅酶A依赖性和丙二酰-ACP无关脂肪酰基辅酶A代谢途径的野生型细胞增加细胞中的酶活性，其中所述脂肪酸含有至少5个碳原子; 和（ii）增加细胞中至少一种蜡酯合酶相对于野生型细胞的活性的第二种遗传突变，并且蜡酯合成酶与SEQ ID NO：1的多肽具有至少50％的序列同一性 -8及其组合或任何多肽的功能片段，用于催化脂肪酸和/或其酰基辅酶A转化为脂肪酸酯。
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4.发明授权Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production 有权β-丙氨酸/α-酮戊二酸氨基转移酶用于3-羟基丙酸生产公开(公告)号：US08030045B2
公开(公告)日：2011-10-04
申请号：US12310503
申请日：2007-08-17
IPC分类号： C12N9/10 , C12N9/00 , C12N1/20 , C12N1/00 , C12N15/00 , C12P7/62 , C12P7/52 , C12P21/06 , C12P19/34 , C07H21/04
CPC分类号： C12P7/42 , C12N9/1096 , C12N15/52 , C12P7/52 , C12Y206/01018
摘要： The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.
摘要翻译： 本公开提供了具有增加的生物活性的新型β-丙氨酸/α-酮戊二酸转氨酶核酸和蛋白质序列。 还提供含有这些酶的细胞，以及它们的使用方法，例如产生丙二酰半醛及其下游产物如3-羟基丙酸及其衍生物。
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公开(公告)号：US20100267115A1
公开(公告)日：2010-10-21
申请号：US12826401
申请日：2010-06-29
CPC分类号： C12N9/90 , C12N9/1096 , C12P7/18 , C12P7/42 , C12P7/62 , C12P7/625 , C12Y206/01018
摘要： Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed.
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公开(公告)号：US20090031453A1
公开(公告)日：2009-01-29
申请号：US11658795
申请日：2004-07-30
申请人： Holly Jean Jessen , Ravi R. Gokarn , Steven John Gort , Ogla V. Selifonova , Hans H. Liao , Brian J. Brazeau
发明人： Holly Jean Jessen , Ravi R. Gokarn , Steven John Gort , Ogla V. Selifonova , Hans H. Liao , Brian J. Brazeau
IPC分类号： C12N9/90 , C12N1/21 , C12N1/15 , C12N1/19 , C12P13/06 , C12P7/42 , C12P7/62 , C12P7/18 , C12P13/00 , C12N15/61 , A01H5/00 , C12N15/63 , C12N5/10
CPC分类号： C12N9/90
摘要： Alanine 2,3-aminomutase sequences are disclosed, as are cells having alanine 2,3-aminomutase activity and methods of selecting for such cells. Methods for producing beta-alanine, pantothenate, 3-hydroxypropionic acid, as well as other organic compounds, are disclosed.
摘要翻译： 公开了丙氨酸2,3-氨基变位酶序列，以及具有丙氨酸2,3-氨基变位酶活性的细胞以及这种细胞的选择方法。 公开了生产β-丙氨酸，泛酸盐，3-羟基丙酸以及其它有机化合物的方法。
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公开(公告)号：US20140349376A1
公开(公告)日：2014-11-27
申请号：US13230217
申请日：2011-09-12
IPC分类号： C12N9/10
CPC分类号： C12P7/42 , C12N9/1096 , C12N15/52 , C12P7/52 , C12Y206/01018
摘要： The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.
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公开(公告)号：US08043841B2
公开(公告)日：2011-10-25
申请号：US12939405
申请日：2010-11-04
摘要： Alanine 2,3-aminomutase sequences are disclosed, as are cells having alanine 2,3-aminomutase activity and methods of selecting for such cells. Methods for producing beta-alanine, pantothenate, 3-hydroxypropionic acid, as well as other organic compounds, are disclosed.
摘要翻译： 公开了丙氨酸2,3-氨基变位酶序列，以及具有丙氨酸2,3-氨基变位酶活性的细胞以及这种细胞的选择方法。 公开了生产β-丙氨酸，泛酸盐，3-羟基丙酸以及其它有机化合物的方法。
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公开(公告)号：US07846707B2
公开(公告)日：2010-12-07
申请号：US12643136
申请日：2009-12-21
摘要： Alanine 2,3-aminomutase sequences are disclosed, as are cells having alanine 2,3-aminomutase activity and methods of selecting for such cells. Methods for producing beta-alanine, pantothenate, 3-hydroxypropionic acid, as well as other organic compounds, are disclosed.
摘要翻译： 公开了丙氨酸2,3-氨基变位酶序列，以及具有丙氨酸2,3-氨基变位酶活性的细胞以及这种细胞的选择方法。 公开了生产β-丙氨酸，泛酸盐，3-羟基丙酸以及其它有机化合物的方法。
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10.发明授权Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase 失效使用β-丙氨酸/丙酮酸氨基转移酶生产3-羟基丙酸公开(公告)号：US07700319B2
公开(公告)日：2010-04-20
申请号：US10581570
申请日：2004-12-06
CPC分类号： C12N9/90 , C12N9/1096 , C12P7/18 , C12P7/42 , C12P7/62 , C12P7/625 , C12Y206/01018
摘要： Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed.
摘要翻译： 公开了使用β-丙氨酸/丙酮酸氨基转移酶从β-丙氨酸产生3-羟基丙酸及其衍生物的方法。 还可以公开可用于实施该方法的细胞和重组核酸。
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