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    Process for making antifusogenic fusion peptides that form inclusion bodies
    2.
    发明申请
    Process for making antifusogenic fusion peptides that form inclusion bodies 有权
    制造形成包涵体的抗融合融合肽的方法

    公开(公告)号：US20050058659A1

    公开(公告)日：2005-03-17

    申请号：US10969624

    申请日：2004-10-20

    申请人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    发明人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P31/12 ,  A61P31/18 ,  C07K14/155 ,  C07K14/16 ,  C12N1/21 ,  C12N15/62 ,  C12P21/02 ,  C12Q1/70 ,  A61K39/21 ,  C07H21/04

    CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12P21/02

    摘要： A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.

    摘要翻译： 公开了通过在原核宿主细胞中产生约14至70个氨基酸长度的融合肽来生产抗融合肽的方法，包括以下步骤：在形成所述融合肽的包涵体的条件下， （a）在所述宿主细胞中表达编码所述融合肽的核酸，所述融合肽由长度为约10至50个氨基酸的抗融合肽的第一肽和长度为约4-30个氨基酸的第二个肽组成 所述第一肽与所述第二肽N-末端连接; （b）培养所述宿主细胞以产生所述包涵体; 和（c）从所述包涵体中回收所述抗融合肽，其中所述回收的抗融合肽由所述融合肽或包含约10至50个氨基酸的抗融合肽的肽组成，并且是从所述融合肽切割的片段。 公开了肽的包涵体。 还公开了编码由第一肽组成的融合肽的核酸，第一肽是选自SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10的抗融合肽， 所述序列进一步由C末端的甘氨酸组成，N-末端与第二肽连接。

    Nucleic acids encoding fusion peptides
    3.
    发明授权
    Nucleic acids encoding fusion peptides 有权
    编码融合肽的核酸

    公开(公告)号：US07348423B2

    公开(公告)日：2008-03-25

    申请号：US10969624

    申请日：2004-10-20

    申请人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    发明人： Eva Hoess ,  Thomas Meier ,  Gabriele Pestlin ,  Friedrich Popp ,  Klaus Reichert ,  Rainer Schmuck ,  Bernd Schneidinger ,  Christoph Seidel ,  Wilhelm Tischer

    IPC分类号： A61K39/21

    CPC分类号： C07K14/005 ,  C07K2319/00 ,  C12N2740/15022 ,  C12N2740/16122 ,  C12P21/02

    摘要： A process is disclosed for the production of an antifusogenic peptide by producing a fusion peptide of a length of about 14 to 70 amino acids in a prokaryotic host cell, comprising the steps, under such conditions that inclusion bodies of said fusion peptide are formed, of: (a) expressing in said host cell a nucleic acid encoding said fusion peptide consisting of a first peptide which is an antifusogenic peptide of a length of about 10 to 50 amino acids and a second peptide of a length of about 4 to 30 amino acids, said first peptide being N-terminally linked to said second peptide; (b) cultivating said host cell to produce said inclusion bodies; and (c) recovering said antifusogenic peptide from said inclusion bodies, wherein said recovered antifusogenic peptide consists of said fusion peptide or a peptide comprising the antifusogenic peptide of about 10 to 50 amino acids and which is a fragment cleaved from said fusion peptide. Inclusion bodies of the peptides are disclosed. Also disclosed is a nucleic acid encoding the fusion peptide consisting of a first peptide which is an antifusogenic peptide selected from the group of SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, and said sequences further consisting of glycine at the C terminal end, N-terminally linked to the second peptide.

    摘要翻译： 公开了通过在原核宿主细胞中产生约14至70个氨基酸长度的融合肽来生产抗融合肽的方法，包括以下步骤：在形成所述融合肽的包涵体的条件下， （a）在所述宿主细胞中表达编码所述融合肽的核酸，所述融合肽由长度为约10至50个氨基酸的抗融合肽的第一肽和长度为约4-30个氨基酸的第二个肽组成 所述第一肽与所述第二肽N-末端连接; （b）培养所述宿主细胞以产生所述包涵体; 和（c）从所述包涵体中回收所述抗融合肽，其中所述回收的抗融合肽由所述融合肽或包含约10至50个氨基酸的抗融合肽的肽组成，并且是从所述融合肽切割的片段。 公开了肽的包涵体。 还公开了编码由第一肽组成的融合肽的核酸，第一肽是选自SEQ ID NO：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10的抗融合肽， 所述序列进一步由C末端的甘氨酸组成，N-末端与第二肽连接。

    Thermostable Taq polymerase fragment
    4.
    发明授权
    Thermostable Taq polymerase fragment 有权
    热稳定性Taq聚合酶片段

    公开(公告)号：US07972830B2

    公开(公告)日：2011-07-05

    申请号：US10917157

    申请日：2004-08-12

    申请人： Thomas Meier ,  Waltraud Ankenbauer ,  Annette Deufel ,  Dieter Heindl ,  Gisela Betzl ,  Rainer Schmuck ,  Bernd Schneidinger ,  Jessica Strey

    发明人： Thomas Meier ,  Waltraud Ankenbauer ,  Annette Deufel ,  Dieter Heindl ,  Gisela Betzl ,  Rainer Schmuck ,  Bernd Schneidinger ,  Jessica Strey

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252

    摘要： It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.

    摘要翻译： 发现缺乏288个N-末端氨基酸（Taq＆Dgr; 288）的天然的水生栖热菌水生DNA聚合酶（TaqWT）的片段具有比TaqWT，Taq＆Dgr; 279和Taq＆Dgr; 289增加的热稳定性。 因此，本发明提供Taq＆Dgr; 288，编码其相同或衍生物的重组表达载体，以及Taq＆Dgr; 288的纯化方案。 本发明还包括含有Taq＆Dgr; 288的试剂盒，以及使用Taq＆Dgr; 288和含有Taq＆Dgr; 288的试剂盒。 此外，本发明包括用于测序核酸模板的方法和用于扩增靶核酸的方法。