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公开(公告)号：US20210010606A1
公开(公告)日：2021-01-14
申请号：US17035220
申请日：2020-09-28
Applicant: FUJIFILM Corporation
Inventor： Yoshihisa USAMI , Yasuyuki ISHII , Yuki INOUE , Aya OUCHI
Abstract: A chip includes a first chamber that stores a fluid, a second chamber that stores a mixing target which is to be mixed with the fluid, a flow path that allows a communication between the first chamber and the second chamber, and a valve that is provided in the flow path and capable of changing the flow path by change in shape by heating.
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公开(公告)号：US20170146514A1
公开(公告)日：2017-05-25
申请号：US15424101
申请日：2017-02-03
Applicant: FUJIFILM Corporation
Inventor： Kenta MATSUBARA , Shinichiro FUKUI , Yasuyuki ISHII
CPC classification number: G01N33/491 , G01N15/1434 , G01N21/27 , G01N21/59 , G01N33/48 , G01N33/483 , G01N33/49 , G01N33/4925 , G01N33/80 , G01N2015/0073 , G01N2015/1006 , G01N2015/149 , G01N2201/12
Abstract: Provided is a method for sorting nucleated red blood cells in which fetus-derived nucleated red blood cells are sorted out from maternal blood. The method includes a nucleated red blood cell specification step of specifying nucleated red blood cells to be sorted out; a reference red blood cell selection step of setting a search range including the nucleated red blood cells and selecting red blood cells having no nucleus within the search range as reference red blood cells; and a sorting step of sorting the nucleated red blood cells into mother-derived nucleated red blood cells and fetus-derived nucleated red blood cells in accordance with spectral characteristics attributable to oxygen affinity of hemoglobin of the nucleated red blood cells and the reference red blood cells.
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公开(公告)号：US20180087112A1
公开(公告)日：2018-03-29
申请号：US15712785
申请日：2017-09-22
Applicant: FUJIFILM Corporation
Inventor： Aya OUCHI , Takayuki TSUJIMOTO , Masaya NAGASE , Yasuyuki ISHII
IPC: C12Q1/6883 , C12N15/10
CPC classification number: C12Q1/6883 , C12N15/09 , C12N15/1089 , C12Q1/6811 , C12Q1/6876 , C12Q2600/156 , C12Q2600/16 , C12Q2535/122 , C12Q2537/165
Abstract: Provided is a method for determining a genetic condition of a fetus, the method including: an objective region selection step of selecting an objective region for determining the genetic condition, from regions on a human genome; a step of isolating a single cell from a maternal blood sample; a step of extracting genomic DNA from the single cell; a step of performing PCR amplification on the objective region using a primer set designed so as to perform the PCR amplification on the objective region using genomic DNA extracted from the single cell as a template; and a DNA sequencing step of decoding a DNA base sequence of a PCR amplification product of the objective region, in which the primer set designed so as to perform the PCR amplification on the objective region is designed through a method for designing a primer set used for the polymerase chain reaction, the method for designing a primer set including a first stage selection step based on a local alignment score and a second stage selection step based on a global alignment score.
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公开(公告)号：US20170152555A1
公开(公告)日：2017-06-01
申请号：US15427050
申请日：2017-02-08
Applicant: FUJIFILM CORPORATION
Inventor： Yuki INOUE , Yasuyuki ISHII , Aya OUCHI
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12N15/09 , C12Q1/68 , C12Q1/6806
Abstract: A method for detecting the presence or absence of heteroploidy of fetal chromosomes includes: obtaining a primary amplification products by amplifying chromosomal DNA obtained from a biological sample collected from a pregnant mother; performing multiplex amplification of a plurality of target regions to obtain a secondary amplification product using the primary amplification product as a template; adding a label to both terminals of the secondary amplification product to obtain the labeled secondary amplification product; performing amplification using a primer pair annealing to the label using the labeled secondary amplification product as a template to obtain a tertiary amplification product; and determining the amplification amount and base sequences of the plurality of target regions from the tertiary amplification product. The primary amplification amplifies the total amount of DNA by 6000 to 30000 times, and the secondary amplification step amplifies the total amount of DNA by 3 to 150 times.
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Patent Agency Ranking
公开(公告)号：US20210023565A1
公开(公告)日：2021-01-28
申请号：US17035370
申请日：2020-09-28
Applicant: FUJIFILM Corporation
Inventor： Yoshihisa USAMI , Yasuyuki ISHII , Yuki INOUE , Aya OUCHI
Abstract: The reaction apparatus includes a cell in which a specimen liquid of 1 μL or more is accommodated, a cooling plate structure that encloses the cell and consists of a cooling plate, a cooling unit that cools the cooling plate structure, and a heating unit that irradiates at least one of the cell or the specimen liquid with electromagnetic waves, in which the cooling plate configuring the cooling plate structure is in contact with at least two main surfaces of the cell, and the cooling plate structure includes a transparent window that transmits the electromagnetic waves.
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6.发明申请公开(公告)号：US20170206310A1
公开(公告)日：2017-07-20
申请号：US15471935
申请日：2017-03-28
Applicant: FUJIFILM Corporation
Inventor： Yasuyuki ISHII , Yuki INOUE , Aya OUCHI
Abstract: The present invention provides a noninvasive discrimination method of chromosomal heteroploidy of a fetus, the method including a step of analyzing DNA of a candidate cell of a nucleated red blood cell isolated from maternal blood, in which the fetuses are fetuses classified into any one case selected from monotocous, monochorionic monoamniotic twin fetuses, monochorionic diamniotic twin fetuses, and dichorionic diamniotic twin fetuses based on results of ultrasonic inspections and the number of candidate cells of nucleated red blood cells to be used for analyzing the DNA is optimized based on the classification, and a noninvasive discrimination system of chromosomal heteroploidy of a fetus.
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公开(公告)号：US20210008569A1
公开(公告)日：2021-01-14
申请号：US17034040
申请日：2020-09-28
Applicant: FUJIFILM Corporation
Inventor： Yoshihisa USAMI , Yasuyuki ISHII , Yuki INOUE , Aya OUCHI
Abstract: A separation device includes: a container that includes a storage portion for storing liquid containing magnetic particles; and a coil that separates the magnetic particles from the liquid by generating a magnetic field through application of current, causing a magnetic force to act on the magnetic particles in a state where the coil stops with respect to the storage portion, and causing the magnetic particles to move from the storage portion against gravity acting on the liquid containing the magnetic particles.
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公开(公告)号：US20170146558A1
公开(公告)日：2017-05-25
申请号：US15424302
申请日：2017-02-03
Applicant: FUJIFILM Corporation
Inventor： Yasuyuki ISHII , Kenta MATSUBARA , Shinichiro FUKUI
CPC classification number: G01N33/80 , C12Q1/02 , C12Q1/68 , C12Q1/6883 , G01N21/27 , G01N21/31 , G01N33/48 , G01N33/483 , G01N33/49
Abstract: A method for sorting nucleated red blood cells, the method includes: selecting a candidate cell of nucleated red blood cells in accordance with shapes of cells; setting a search range including the candidate cell and selecting a white blood cell within the search range; defining that the candidate cell is a nucleated red blood cell through comparison of spectral characteristics of hemoglobin between the candidate cell and the white blood cell; and sorting the nucleated red blood cells into mother-derived nucleated red blood cells or fetus-derived nucleated red blood cells through comparison of an allele contained in the nucleated red blood cell after the defining of the nucleated red blood cell with an allele contained in a white blood cell which has not been selected in the selecting of the candidate cell or in the white blood cell selected in the selecting of the white blood cell.
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公开(公告)号：US20170145512A1
公开(公告)日：2017-05-25
申请号：US15423998
申请日：2017-02-03
Applicant: FUJIFILM Corporation
Inventor： Shinichiro FUKUI , Yasuyuki ISHII , Kenta MATSUBARA
CPC classification number: C12Q1/6883 , C12Q1/04 , C12Q1/68 , G01N1/4077 , G01N15/1475 , G01N21/27 , G01N21/31 , G01N33/48 , G01N33/49 , G01N2001/4083 , G01N2015/0073 , G01N2015/1006 , G01N2015/149 , G01N2021/3133
Abstract: A method for inspecting a chromosome of a fetus includes: a collection step of obtaining maternal blood from a pregnant mother; a concentration step of concentrating nucleated red blood cells in the maternal blood; a sorting step of sorting the nucleated red blood cells in the maternal blood, in which the nucleated red blood cells are concentrated through the concentration step, into mother-derived nucleated red blood cells and fetus-derived nucleated red blood cells in accordance with the shapes of nuclei and spectral characteristics at wavelengths included in a light wavelength region of 400 to 650 nm; an amplification step of amplifying a nucleic acid of a chromosome of the nucleated red blood cells; a definition step of defining an amount of the amplified product of the nucleated red blood cells; and a determination step of determining presence or absence of a numerical abnormality of the fetus-derived chromosome.
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