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    Methods for conducting assays for enzyme activity on protein microarrays
    2.
    发明申请
    Methods for conducting assays for enzyme activity on protein microarrays 有权
    蛋白质微阵列酶活性测定方法

    公开(公告)号：US20050118665A1

    公开(公告)日：2005-06-02

    申请号：US10865431

    申请日：2004-06-09

    申请人： Fang Zhou ,  Barry Schweitzer

    发明人： Fang Zhou ,  Barry Schweitzer

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  C12Q1/37

    CPC分类号： C12Q1/00

    摘要： The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.

    摘要翻译： 本发明涉及对用于蛋白质功能的大规模研究，筛选测定和酶促反应的高通量分析的微阵列上的酶活性进行测定的方法。 本发明涉及使用蛋白质芯片来测定存在于蛋白质芯片上的蛋白质样品中的酶的存在，量，活性和/或功能的方法。 特别地，本发明的方法涉及使用微阵列进行酶测定，其中将蛋白质和物质固定在固体支持物的表面上，并且其中所述蛋白质和所述物质彼此接近，足以发生 物质与蛋白质之间的酶反应。 本发明还涉及具有固定在其表面上的酶和底物的微阵列，其中酶和底物相互接近，足以在酶和底物之间发生酶反应。

    Charged particle beam emitting device and method for operating a charged particle beam emitting device
    4.
    发明授权
    Charged particle beam emitting device and method for operating a charged particle beam emitting device 有权
    带电粒子束发射装置和用于操作带电粒子束发射装置的方法

    公开(公告)号：US07501638B1

    公开(公告)日：2009-03-10

    申请号：US11553160

    申请日：2006-10-26

    申请人： Fang Zhou

    发明人： Fang Zhou

    IPC分类号： H01J37/10 ,  H01J37/147

    CPC分类号： H01J9/505 ,  H01J3/06 ,  H01J37/067 ,  Y02W30/828

    摘要： A charged particle beam emitting device includes at least two charged particle beam guns, each of the at least two charged particle beam guns having a separate charged particle emitter with an emitting surface for emitting a respective charged particle beam. The charged particle beam emitting device further includes an aperture element comprising at least one aperture opening and a deflector unit. The deflector unit is adapted for alternatively directing the charged particle beams of the at least two charged particle beam guns on the at least one aperture opening so that, at the same time, one of the at least two charged particle beams is directed on the aperture opening while the respective other charged particle beam of the at least two charged particle beams is deflected from the aperture opening by the deflector unit. At the same time, only one of the two charged particle beam guns is used so that the temporarily unused charged particle beam gun can be subjected to a cleaning procedure. This ensures that the emitting surfaces of both charged particle beam guns can be alternatively and frequently cleaned with minimum interruption of the operation of the charged particle beam device.

    摘要翻译： 带电粒子束发射装置包括至少两个带电粒子束枪，所述至少两个带电粒子束枪中的每一个具有分离的带电粒子发射器，具有用于发射相应带电粒子束的发射表面。 带电粒子束发射装置还包括孔元件，该孔元件包括至少一个孔口和偏转器单元。 偏转器单元适于将至少两个带电粒子束枪的带电粒子束交替地引导到至少一个孔口上，使得同时，至少两个带电粒子束中的一个被引导到孔 所述至少两个带电粒子束的相应的其它带电粒子束由所述偏转器单元从所述开口偏转。 同时，仅使用两个带电粒子束枪中的一个，使得临时使用的带电粒子束枪可以进行清洁程序。 这确保了两个带电粒子束枪的发射表面可以以最小的带电粒子束装置的操作中断来交替地和频繁地清洁。

    Field emitter arrangement and method of cleansing an emitting surface of a field emitter
    9.
    发明申请
    Field emitter arrangement and method of cleansing an emitting surface of a field emitter 审中-公开
    场发射器布置和清洁场发射器的发射表面的方法

    公开(公告)号：US20070018562A1

    公开(公告)日：2007-01-25

    申请号：US11489979

    申请日：2006-07-20

    申请人： Pavel Adamec ,  Fang Zhou

    发明人： Pavel Adamec ,  Fang Zhou

    IPC分类号： H01J1/02

    CPC分类号： H01J37/06 ,  H01J37/02 ,  H01J37/073 ,  H01J49/48 ,  H01J2237/06316

    摘要： A field emitter arrangement and a method of cleaning an emitting surface of a field emitter are provided. The field emitter arrangement may include a field emitter tip having an emitting surface, wherein said field emitter tip is adapted to generate a primary beam of charged particles, and at least one electron source adapted to illuminate the emitting surface of the field emitter tip. The method of cleaning the emitting surface may include providing the field emitter having the emitting surface and at least one electron source adapted to illuminate the emitting surface and illuminating the emitting surface of the field emitter with a cleansing electron beam generated by the at least one electron source.

    摘要翻译： 提供场发射器布置和清洁场发射器的发射表面的方法。 场发射器配置可以包括具有发射表面的场发射器尖端，其中所述场发射极尖端适于产生带电粒子的主光束，以及适于照射场发射器尖端的发射表面的至少一个电子源。 清洁发射表面的方法可以包括提供具有发射表面的场致发射体和至少一个电子源，其适于照射发射表面并用由至少一个电子产生的清洁电子束照射场发射器的发射表面 资源。

    Multi-segments Rotor Blade of Wind Turbine
    10.
    发明申请
    Multi-segments Rotor Blade of Wind Turbine 审中-公开

    公开(公告)号：US20190285047A1

    公开(公告)日：2019-09-19

    申请号：US16300939

    申请日：2016-05-13

    申请人： Fang ZHOU

    发明人： Fang Zhou

    IPC分类号： F03D1/06 ,  F03D13/10 ,  F03D13/40

    摘要： The present invention provides a variable-pitch blade component, which comprising: blades with multiple blade segments, at least two adjacent blade segments are capable of mutually rotating so as to change its pitch angle; and the variable-pitch guiding structure, comprising the circumferential-extension guide rail fixed to one of the two adjacent blade segments that are capable of mutually rotating, and one or multiple guiding elements fixed to another one of the two adjacent blade segments which are capable of mutually rotating, wherein said one or multiple guiding elements are constructed to be guided and moved on said guide rail.