公开(公告)号：US09155980B2

公开(公告)日：2015-10-13

申请号：US13771302

申请日：2013-02-20

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman

IPC: B01D15/38 ,  B01D15/36 ,  B01J20/28 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32 ,  C07K1/16 ,  C07K1/22 ,  C07K16/00 ,  B01D15/16

CPC classification number: B01D15/3828 ,  B01D15/166 ,  B01D15/36 ,  B01J20/28092 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3242 ,  B01J20/3293 ,  C07K1/16 ,  C07K1/22 ,  C07K16/00

Abstract: The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.

Abstract translation: 本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与 盖中配体的电荷。 本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。

公开(公告)号：US09878266B2

公开(公告)日：2018-01-30

申请号：US14542816

申请日：2014-11-17

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Carina Engstrand ,  Annika Forss ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Hans J Johansson ,  Jean-Luc Maloisel

IPC: C07K1/14 ,  B01D15/38 ,  B01D15/32 ,  B01D15/36 ,  B01J20/286 ,  B01J20/32 ,  B01J41/20 ,  C07K1/36 ,  C07K16/06 ,  C07K1/16 ,  C07K16/00

CPC classification number: B01D15/3828 ,  B01D15/327 ,  B01D15/363 ,  B01D15/3804 ,  B01D15/3809 ,  B01D15/3847 ,  B01J20/286 ,  B01J20/3253 ,  B01J20/3285 ,  B01J41/20 ,  C07K1/165 ,  C07K1/36 ,  C07K16/00 ,  C07K16/065

Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.

公开(公告)号：US20150299248A1

公开(公告)日：2015-10-22

申请号：US14441129

申请日：2013-11-11

Applicant: GE HEALTHCARE BIO-SCIENCES AB

Inventor： Jean-Luc Maloisel ,  Gustav Rodrigo ,  Bjorn Noren ,  Virendra Kumbhar

IPC: C07K1/16 ,  C07K1/36 ,  B01J20/289 ,  B01J20/26 ,  B01J20/28

CPC classification number: C07K1/165 ,  B01D15/3847 ,  B01D15/3885 ,  B01J20/265 ,  B01J20/28016 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3253 ,  B01J20/3255 ,  B01J20/3285 ,  B01J41/20 ,  B01J2220/80 ,  C07K1/36 ,  G01N33/544 ,  B01D15/363 ,  B01D15/327

Abstract: The invention discloses a separation matrix which comprises a plurality of separation ligands, defined by the formula R1-L1-N(R3)-L2-R, immobilized on a support, wherein R1 is a five- or six-membered, substituted or non-substituted ring structure or a hydroxyethyl or hydroxypropyl group; L1 is either a methylene group or a covalent bond; R2 is a five- or six-membered, substituted or non-substituted ring structure; L2 is either a methylene group or a covalent bond; R3 is a methyl group; and wherein if R1 is a hydroxyethyl group and L1 is a covalent bond, R2 is a substituted aromatic ring structure or a substituted or non-substituted aliphatic ring structure.

Abstract translation: 本发明公开了一种分离基质，其包含固定在载体上的式R1-L1-N（R3）-L2-R定义的多个分离配体，其中R1是五或六元的取代或非取代的 取代的环结构或羟乙基或羟丙基; L1是亚甲基或共价键; R2是五或六元，取代或未取代的环结构; L2是亚甲基或共价键; R3是甲基; 并且其中如果R 1是羟乙基并且L 1是共价键，则R 2是取代的芳环结构或取代或未取代的脂族环结构。