-
公开(公告)号：US09433923B2
公开(公告)日：2016-09-06
申请号：US14407892
申请日：2013-06-11
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Gunnar Glad , Therese Graner , Nils Norrman
IPC: C12Q1/00 , B01J20/291 , C07K1/22 , B01D15/38 , B01J20/288 , B01J20/32 , B01J20/28 , B01J20/26 , C12N9/10
CPC classification number: B01J20/291 , B01D15/3804 , B01D15/3885 , B01D2015/3838 , B01J20/265 , B01J20/28009 , B01J20/288 , B01J20/3212 , B01J20/3251 , B01J20/3274 , B01J2220/80 , C07K1/22 , C07K2319/23 , C12N9/1088
Abstract: The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins.
Abstract translation: 本发明涉及提供有或不具有磁性颗粒的谷胱甘肽配体的新型非交联色谱介质。 色谱介质用于生产具有用于吸附GST标记的蛋白质的凝胶配体的亲和介质。
-
公开(公告)号：US09155980B2
公开(公告)日：2015-10-13
申请号：US13771302
申请日：2013-02-20
Applicant: GE HEALTHCARE BIO-SCIENCES AB
Inventor： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
IPC: B01D15/38 , B01D15/36 , B01J20/28 , B01J20/285 , B01J20/286 , B01J20/32 , C07K1/16 , C07K1/22 , C07K16/00 , B01D15/16
CPC classification number: B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
Abstract: The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
Abstract translation: 本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与 盖中配体的电荷。 本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。
-
公开(公告)号：US20150151276A1
公开(公告)日：2015-06-04
申请号：US14407892
申请日：2013-06-11
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Gunnar Glad , Therese Graner , Nils Norrman
CPC classification number: B01J20/291 , B01D15/3804 , B01D15/3885 , B01D2015/3838 , B01J20/265 , B01J20/28009 , B01J20/288 , B01J20/3212 , B01J20/3251 , B01J20/3274 , B01J2220/80 , C07K1/22 , C07K2319/23 , C12N9/1088
Abstract: The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins.
Abstract translation: 本发明涉及提供有或不具有磁性颗粒的谷胱甘肽配体的新型非交联色谱介质。 色谱介质用于生产具有用于吸附GST标记的蛋白质的凝胶配体的亲和介质。
-
公开(公告)号：US09878266B2
公开(公告)日：2018-01-30
申请号：US14542816
申请日：2014-11-17
Applicant: GE HEALTHCARE BIO-SCIENCES AB
Inventor： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans J Johansson , Jean-Luc Maloisel
IPC: C07K1/14 , B01D15/38 , B01D15/32 , B01D15/36 , B01J20/286 , B01J20/32 , B01J41/20 , C07K1/36 , C07K16/06 , C07K1/16 , C07K16/00
CPC classification number: B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
Abstract: The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
-
-
-
Search Results
4
records
(took 0.013 seconds)
