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公开(公告)号：US11846635B2
公开(公告)日：2023-12-19
申请号：US16470677
申请日：2017-12-21
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Ola Lind , Klaus Gebauer , Eric Faldt , Nils Norrman , Ronnie Palmgren , Jesper Hedberg , Niklas Jungnelius , Karl Liderfelt , Anita Solbrand
IPC: G01N33/543 , C12M3/00 , C12M3/06 , C12M1/00 , C12M1/34 , C12N13/00 , B03C5/00 , B03C5/02 , B01D21/00 , B03C1/30 , B03C1/01 , B03C1/28
CPC classification number: G01N33/54326 , B01D21/0009 , B03C1/30 , B03C5/00 , B03C5/02 , C12M23/48 , C12M27/16 , C12M29/00 , C12M41/44 , C12M45/07 , C12M47/04 , C12M47/10 , C12N13/00 , G01N33/5436 , B03C1/01 , B03C1/288 , B03C2201/26 , G01N2333/31
Abstract: The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.
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公开(公告)号：US20200087614A1
公开(公告)日：2020-03-19
申请号：US16470607
申请日：2017-12-27
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Ola Lind , Johan Fredrik Avallin , Andreas Torbjorn Lundin , Nils Norrman , Ronnie Palmgren , Gustav Jose Rodrigo
Abstract: A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.
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公开(公告)号：US08844385B2
公开(公告)日：2014-09-30
申请号：US13857646
申请日：2013-04-05
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Nils Norrman , Ronnie Palmgren
CPC classification number: G01N30/84 , B01L3/0275 , B01L3/508 , B01L2200/026 , B01L2300/0681 , B01L2400/0605 , G01N30/6065 , G01N2030/009 , G01N2030/062
Abstract: The present invention relates to a device and method for separation of proteins and other biomolecules. A preferred use is for sample preparation of crude as well as pre-fractionated samples. In a preferred embodiment, the device of the present invention is a pipette tip having dual channels, one for inlet of sample and one for outlet. The outlet, but not the inlet, channel is provided with sample separation media for separation of a desired biomolecule from a sample. The flow through the sample separation media is unidirectional.
Abstract translation: 本发明涉及分离蛋白质和其他生物分子的装置和方法。 优选的用途是用于样品制备粗品以及预先分馏的样品。 在优选实施例中，本发明的装置是具有双通道的移液管尖端，一个用于样品入口，一个用于出口。 出口但不是入口通道设置有样品分离介质，用于将期望的生物分子与样品分离。 通过样品分离介质的流动是单向的。
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公开(公告)号：US11686726B2
公开(公告)日：2023-06-27
申请号：US16470607
申请日：2017-12-27
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Ola Lind , Johan Fredrik Avallin , Andreas Torbjorn Lundin , Nils Norrman , Ronnie Palmgren , Gustav Jose Rodrigo
IPC: G01N33/543 , C12M3/00 , C12M3/06 , C12M1/00 , C12M1/34 , C12N13/00 , B01D21/00 , B03C5/00 , B03C5/02 , B03C1/30 , B03C1/28 , B03C1/01
CPC classification number: G01N33/54326 , B01D21/0009 , B03C1/30 , B03C5/00 , B03C5/02 , C12M23/48 , C12M27/16 , C12M29/00 , C12M41/44 , C12M45/07 , C12M47/04 , C12M47/10 , C12N13/00 , G01N33/5436 , B03C1/01 , B03C1/288 , B03C2201/26 , G01N2333/31
Abstract: A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: —providing a feed from a cell culture (3; 103; 203) comprising said biomolecule to a magnetic separator (5; 105; 205) and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; —separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; —forwarding said magnetic beads as a slurry with an added buffer to an elution cell (7; 107; 207); —eluting the bound biomolecules in the elution cell.
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公开(公告)号：US10429359B2
公开(公告)日：2019-10-01
申请号：US15116940
申请日：2015-02-13
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Christer Olof Eriksson , Nils Norrman
IPC: G01N30/04 , B01D15/34 , G01N30/46 , B01D15/12 , B01D15/16 , B01D15/18 , B01D15/20 , B01D15/38 , G01N30/88
Abstract: Automated two step chromatography purification system comprising a, system controller, a capture flow path comprising at least one pump, an elution flow path comprising at least one pump, and a valve arrangement for selective connection of two capture columns to the capture flow path and the elution flow path respectively such that both flow paths may be operated simultaneously and in parallel.
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Patent Agency Ranking
公开(公告)号：US10421074B2
公开(公告)日：2019-09-24
申请号：US15101234
申请日：2014-11-25
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Nils Norrman , Henrik Ostlin
IPC: B01L3/00
Abstract: The present invention provides a microplate comprised of multiwell strips connected in such a way as to permit the microplate to unfold and to pass in a linear fashion past a fraction collector and then re-fold back into a microplate configuration. Furthermore, the invention provides for the use of the microplates of the invention in a variety of different laboratory methods.
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公开(公告)号：US20160303559A1
公开(公告)日：2016-10-20
申请号：US15101337
申请日：2014-11-26
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Tobias Ingemar Solderman , Nils Norrman , Henrik Ostlin , Susanna Lindman , David Carl Martin Bergman , Peter Mats OLIVIUSSON
IPC: B01L3/00
CPC classification number: B01L3/5025 , B01L3/502 , B01L3/5085 , B01L3/50855 , B01L2200/028 , B01L2200/0621 , B01L2200/0631 , B01L2300/0803 , B01L2300/0858 , B01L2300/0864 , B01L2300/087 , B01L2300/0887 , B01L2400/0406 , B01L2400/0478 , G01N21/07 , G01N27/44739 , G01N35/00 , G01N35/1065 , G01N2035/00108 , G01N2035/0439
Abstract: The present invention provides methods and systems for eluting and an analyte from a solid phase. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a solid phase to a second vessel, such as a microtitre plate well. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.
Abstract translation: 本发明提供用于从固相洗脱和分析物的方法和系统。 本发明还提供用于将液体分析物试剂混合物从固相转移到第二容器（例如微量滴定板）的方法和系统。 本发明可用于操纵核酸，碳水化合物，蛋白质和肽等生物分子。 特别地，本发明可用于在等电聚焦凝胶中操作蛋白质和肽。
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公开(公告)号：US09433923B2
公开(公告)日：2016-09-06
申请号：US14407892
申请日：2013-06-11
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Gunnar Glad , Therese Graner , Nils Norrman
IPC: C12Q1/00 , B01J20/291 , C07K1/22 , B01D15/38 , B01J20/288 , B01J20/32 , B01J20/28 , B01J20/26 , C12N9/10
CPC classification number: B01J20/291 , B01D15/3804 , B01D15/3885 , B01D2015/3838 , B01J20/265 , B01J20/28009 , B01J20/288 , B01J20/3212 , B01J20/3251 , B01J20/3274 , B01J2220/80 , C07K1/22 , C07K2319/23 , C12N9/1088
Abstract: The present invention relates to a novel non-cross linked chromatography media provided with glutathione ligands which may or may not be provided with magnetic particles. The chromatography media is used for production of an affinity media provided with gluthatione ligands for adsorption of GST-tagged proteins.
Abstract translation: 本发明涉及提供有或不具有磁性颗粒的谷胱甘肽配体的新型非交联色谱介质。 色谱介质用于生产具有用于吸附GST标记的蛋白质的凝胶配体的亲和介质。
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公开(公告)号：US09155980B2
公开(公告)日：2015-10-13
申请号：US13771302
申请日：2013-02-20
Applicant: GE HEALTHCARE BIO-SCIENCES AB
Inventor： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
IPC: B01D15/38 , B01D15/36 , B01J20/28 , B01J20/285 , B01J20/286 , B01J20/32 , C07K1/16 , C07K1/22 , C07K16/00 , B01D15/16
CPC classification number: B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
Abstract: The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
Abstract translation: 本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与 盖中配体的电荷。 本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。
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公开(公告)号：US20190339261A1
公开(公告)日：2019-11-07
申请号：US16470677
申请日：2017-12-21
Applicant: GE Healthcare Bio-Sciences AB
Inventor： Ola Lind , Klaus Gebauer , Eric Faldt , Nils Norrman , Ronnie Palmgren , Jesper Hedberg , Niklas Jungnelius , Karl Liderfelt , Anita Solbrand
IPC: G01N33/543 , C12M1/00 , C12M3/00 , C12M3/06 , C12M1/34
Abstract: The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.
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