公开(公告)号：US20240240160A1

公开(公告)日：2024-07-18

申请号：US18564967

申请日：2022-05-27

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Michael Angus NOBLE ,  Jamison Parker HUDDLESTON

IPC: C12N9/10 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32

CPC classification number: C12N9/1029 ,  C12N15/52 ,  C12N15/70 ,  C12P7/22 ,  C12P7/42 ,  C12P17/06 ,  C12P19/32 ,  C12Y203/01206

Abstract: The present disclosure provides a poly nucleotide comprising: (a) a nucleic acid sequence encoding an olivetol synthase (OLS): and (b) a heterologous regulatory element operably linked to the nucleic acid sequence. The present disclosure further relates to an engineered cell comprising an olivetol synthase (OLS). Also provided are a cell extract or cell culture medium or a composition comprising 3.5.7- trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof, and a method of making 3.5.7-trioxododecanoyl-CoA, olivetol, olivetolic acid, a cannabinoid, and/or an isomer, analog, or derivative thereof. Also provided is a cannabinoid produced by the engineered cell, isolated from the cell extract or cell culture medium, and/or made by the method described herein. The present disclosure also provides a non-natural olivetol synthase (OLS).

公开(公告)号：US20230167468A1

公开(公告)日：2023-06-01

申请号：US17995778

申请日：2021-04-13

Applicant: GENOMATICA, INC.

Inventor： Deqiang ZHANG ,  Jamison Parker HUDDLESTON ,  Joseph Roy WARNER ,  Benjamin Matthew GRIFFIN

IPC: C12P7/42 ,  C12N9/02 ,  C12P17/06 ,  C12P7/22

CPC classification number: C12P7/42 ,  C12N9/0004 ,  C12Y121/03008 ,  C12Y121/03007 ,  C12P17/06 ,  C12P7/22

Abstract: The invention relates to a non-natural cannabinoid synthase comprising at least one amino acid variation as compared to a wild type cannabinoid synthase Δ9-tetrahydrocannabinolic acid synthase (THCAS), comprising three alpha helices (αA, αB and αC) where a disulfide bond is not formed between alpha helix αA and alpha helix αC, wherein the non-natural cannabinoid synthase catalyzes the oxidative cyclization of cannabigerolic acid (CBGA) into a cannabinoid. The invention further relates to a non-natural Δ9-tetrahydrocannabinolic acid synthase (THCAS), a non-natural cannabidiolic acid synthase (CBDAS), and a non-natural cannabichromenic acid synthase (CBCAS) comprising at least one amino acid variation as compared to a wild type THCAS, CBDAS, or CBCAS, respectively, comprising three alpha helices (αA, αB and αC) and wherein a disulfide bond is not formed between alpha helix αA and alpha helix αC. The invention also relates to a nucleic acid, expression construct, and engineered cell for making the non-natural THCAS, CBDAS, and/or CBCAS. Also provided are compositions comprising the non-natural THCAS, CBDAS, and/or CBCAS; isolated non-natural THCAS, CBDAS, and/or CBCAS enzymes; methods of making the isolated enzymes; cell extracts comprising cannabinoids; and methods of making cannabinoids.

公开(公告)号：US20230332193A1

公开(公告)日：2023-10-19

申请号：US18043258

申请日：2021-09-01

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Deqiang ZHANG ,  Jamison Parker HUDDLESTON

IPC: C12P17/06 ,  C12N9/04 ,  C12N9/10 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P17/06 ,  C12N9/0006 ,  C12N9/1029 ,  C12Y203/01206 ,  C12N9/88 ,  C12Y404/01026 ,  C12N9/1085 ,  C12Y205/0101 ,  C12N15/52

Abstract: The disclosure relates to a non-natural flavin-dependent oxidase comprising at least one amino acid variation as compared to a wild type flavin-dependent oxidase, wherein the non-natural flavin-dependent oxidase does not comprise a disulfide bond, and wherein the non-natural flavin-dependent oxidase is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. The disclosure also relates to a nucleic acid, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are compositions comprising the non-natural flavin-dependent oxidase; isolated non-natural flavin-dependent oxidase and methods of making the same; cell extracts comprising the non-natural flavin-dependent oxidase; and methods of making cannabinoids.