公开(公告)号：US20190010479A1

公开(公告)日：2019-01-10

申请号：US15578488

申请日：2016-05-26

Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

Inventor： Stephanie J. CULLER ,  Robert J. HASELBECK ,  Harish NAGARAJAN ,  Iuri Estrada GOUVEA ,  Daniel Johannes KOCH ,  Mateus Schreiner Garcez LOPES ,  Lucas Pedersen PARIZZI

IPC: C12N9/88 ,  C12P7/16 ,  C07K16/40 ,  C12N15/82 ,  C08F36/06 ,  C12P5/02

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

公开(公告)号：US20200283811A1

公开(公告)日：2020-09-10

申请号：US16796426

申请日：2020-02-20

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pederson PARIZZI ,  Felipe GALZERANI ,  Jean Marie FRANCOIS ,  Sophie LAJUS

IPC: C12P17/18 ,  C12P17/02 ,  C12N15/52 ,  C12N1/20 ,  C12P7/28 ,  C12P5/02

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) or glycolic acid (GA), or MEG and one or more co-product, from one or more pentose and/or hexose sugars. Also provided are methods of producing MEG (or GA), or MEG (or GA) and one or more co-product, from one or more pentose and/or hexose sugars using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA), or MEG and one or more co-product.

公开(公告)号：US20200283806A1

公开(公告)日：2020-09-10

申请号：US16796417

申请日：2020-02-20

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pedersen PARIZZI ,  Felipe GALZERANI

IPC: C12P7/18 ,  C12P17/02 ,  C12N15/52 ,  C12N1/20 ,  C12P7/28 ,  C12P5/02

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US20240392329A1

公开(公告)日：2024-11-28

申请号：US18737619

申请日：2024-06-07

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Lucas Pedersen PARIZZI ,  Felipe GALZERANI

IPC: C12P7/18 ,  C12N1/20 ,  C12N15/52 ,  C12P5/02 ,  C12P7/28 ,  C12P17/02 ,  C12R1/19

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US20200263210A1

公开(公告)日：2020-08-20

申请号：US16791556

申请日：2020-02-14

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Felipe GALZERANI ,  Paulo Moises Raduan ALEXANDRINO

IPC: C12P7/42 ,  C12P13/04 ,  C12N9/00 ,  C12N9/88

Abstract: The present invention provides biochemical pathways, glyoxylate producing recombinant microorganisms, and methods for the production and yield improvement of glycolic acid and/or glycine via a reverse glyoxylate shunt. The reverse glyoxylate shunt comprises an enzyme that catalyzes the carboxylation of phosphoenol pyruvate (PEP) to oxaloacetate (OAA), or an enzyme that catalyzes the carboxylation of pyruvate to oxaloacetate (OAA) or an enzyme that catalyzes the carboxylation of pyruvate to malate or a combination of any of the previous reactions; an enzyme that catalyzes the conversion of malate to malyl-CoA; an enzyme that catalyzes the conversion of malyl-CoA to glyoxylate and acetyl-CoA; and optionally an enzyme that catalyzes the conversion of oxaloacetate (OAA) to malate. Glyoxylate is reduced to produce glycolate. Alternatively, glyoxylate is converted to glycine. The reverse glyoxylate shunt pathway of the present invention can be utilized synergistically with other glycolic acid and/or glycine producing pathways to increase product yield.

公开(公告)号：US20200048662A1

公开(公告)日：2020-02-13

申请号：US16341283

申请日：2017-10-11

Applicant: Braskem S.A.

Inventor： Felipe GALZERANI ,  Daniel Johannes KOCH ,  Mateus Schreiner LOPES

IPC: C12P7/18 ,  C12P5/02 ,  C12N15/70 ,  C12N9/90 ,  C12N9/12

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and isobutene. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and isobutene. Also provided are methods of producing MEG and isobutene using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and isobutene.

公开(公告)号：US20190323016A1

公开(公告)日：2019-10-24

申请号：US16317406

申请日：2017-07-12

Applicant: BRASKEM S.A.

Inventor： Mateus Schreiner LOPES ,  Daniel Johannes KOCH ,  Iuri Estrada GOUVEA ,  Debora Noma OKAMOTO ,  Veronica Leite QUEIROZ

IPC: C12N15/52 ,  C12N9/88 ,  C12N9/90 ,  C12P5/02 ,  C12P7/04

Abstract: The present application relates to recombinant microorganisms expressing a dehydratase useful in a one-step, direct fermentative production of one or more primary alkenes from one or more saturated primary or secondary alcohols. Known, well developed high-yielding pathways that use renewable feedstock can be introduced into the recombinant microorganisms to obtain the alcohol precursors. Also provided are methods of producing one or more primary alkenes using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the primary alkene products.

公开(公告)号：US20180023101A1

公开(公告)日：2018-01-25

申请号：US15726983

申请日：2017-10-06

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Mateus Schreiner Lopes ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12P7/28 ,  C12N9/02 ,  C12N9/88 ,  C12N9/12 ,  C12P7/18 ,  C12N15/52 ,  C12P5/02 ,  C12P7/04 ,  C12P7/26 ,  C12N9/10 ,  C12N9/90

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.

公开(公告)号：US20170260551A1

公开(公告)日：2017-09-14

申请号：US15453094

申请日：2017-03-08

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Mateus Schreiner LOPES ,  Ane Fernanda Beraldi ZEIDLER ,  Lucas Pedersen PARIZZI

IPC: C12P7/28 ,  C12P7/26 ,  C12P7/04 ,  C12P5/02 ,  C12N9/10 ,  C12N9/90 ,  C12N9/12 ,  C12N9/88 ,  C12N9/02 ,  C12P7/18 ,  C12N15/52

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.