公开(公告)号：US10941454B2

公开(公告)日：2021-03-09

申请号：US15578488

申请日：2016-05-26

Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

Inventor： Stephanie J. Culler ,  Robert J. Haselbeck ,  Harish Nagarajan ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Mateus Schreiner Garcez Lopes ,  Lucas Pedersen Parizzi

IPC: C12N9/88 ,  C12N9/90 ,  C07K16/40 ,  C08F36/06 ,  C12N15/82 ,  C12P5/02 ,  C12P7/16

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

公开(公告)号：US12006526B2

公开(公告)日：2024-06-11

申请号：US16796417

申请日：2020-02-20

Applicant: Braskem S.A.

Inventor： Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Felipe Galzerani

IPC: C12N1/20 ,  C12N15/52 ,  C12P5/02 ,  C12P7/18 ,  C12P7/28 ,  C12P17/02 ,  C12R1/19

CPC classification number: C12P7/18 ,  C12N1/20 ,  C12N15/52 ,  C12P5/026 ,  C12P7/28 ,  C12P17/02 ,  C12N1/205 ,  C12R2001/19 ,  C12Y101/01043 ,  C12Y101/01301 ,  C12Y102/01009 ,  C12Y203/01008 ,  C12Y207/01011 ,  C12Y207/02003 ,  C12Y301/01031 ,  C12Y401/02022 ,  C12Y501/03001 ,  C12Y503/01006 ,  C12Y504/02

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG), or optionally MEG and one or more co-product, from one or more hexose feedstock. The present application also relates to recombinant microorganisms useful in the biosynthesis of glycolic acid (GA), or optionally GA and one or more co-product, from one or more hexose feedstock. The present application relates to recombinant microorganisms useful in the biosynthesis of xylitol, or optionally xylitol and one or more co-product, from one or more hexose feedstock. Also provided are methods of producing MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product, from one or more hexose feedstock using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or the products MEG (or GA or xylitol), or optionally MEG (or GA or xylitol) and one or more co-product.

公开(公告)号：US11530391B2

公开(公告)日：2022-12-20

申请号：US16378209

申请日：2019-04-08

Applicant: Braskem S.A.

Inventor： Verônica Leite Queiroz ,  Lucas Pedersen Parizzi ,  Iuri Estrada Gouvea ,  Debora Noma Okamoto ,  Rafael Victório Carvalho Guido ,  Alessandro Silva Nascimento ,  Igor Polikarpov

IPC: C12N9/04 ,  C12N15/30 ,  C12N15/53 ,  C12N15/52

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US12037609B2

公开(公告)日：2024-07-16

申请号：US18084888

申请日：2022-12-20

Applicant: Braskem S.A.

Inventor： Verônica Leite Queiroz ,  Lucas Pedersen Parizzi ,  Iuri Estrada Gouvea ,  Debora Noma Okamoto ,  Rafael Victório Carvalho Guido ,  Alessandro Silva Nascimento ,  Igor Polikarpov

IPC: C12N9/04 ,  C12N1/21 ,  C12N15/52

CPC classification number: C12N9/0006 ,  C12N15/52 ,  C12Y101/01001

Abstract: The present disclosure relates to biological processes and systems for the production of isopropanol and/or acetone utilizing modified alcohol dehydrogenases that exhibit increased activity with NADH as a cofactor. The disclosure further relates to polynucleotides and polypeptides of the modified alcohol dehydrogenases, and host cells containing the polynucleotides and expressing the polypeptides.

公开(公告)号：US10774348B2

公开(公告)日：2020-09-15

申请号：US15726983

申请日：2017-10-06

Applicant: Braskem S.A.

Inventor： Daniel Johannes Koch ,  Mateus Schreiner Lopes ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12N9/10 ,  C12N1/20 ,  C12P7/28 ,  C12P7/18 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/02 ,  C12P7/04 ,  C12P7/26

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.

公开(公告)号：US10774347B2

公开(公告)日：2020-09-15

申请号：US15453094

申请日：2017-03-08

Applicant: Braskem S.A.

Inventor： Daniel Johannes Koch ,  Mateus Schreiner Lopes ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12N9/10 ,  C12N1/20 ,  C12P7/28 ,  C12P7/18 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/02 ,  C12P7/04 ,  C12P7/26

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.

公开(公告)号：US10273505B2

公开(公告)日：2019-04-30

申请号：US16042594

申请日：2018-07-23

Applicant: Braskem S.A.

Inventor： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC: C12P5/02 ,  C12N15/52 ,  C12N9/88

Abstract: The present disclosure generally relates to methods of using microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene and products and processes derived therefrom.

公开(公告)号：US10059963B2

公开(公告)日：2018-08-28

申请号：US14885311

申请日：2015-10-16

Applicant: Braskem S.A.

Inventor： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC: C12P5/02 ,  C12N15/52 ,  C12N9/88

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y402/01127

Abstract: The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.

公开(公告)号：US20150064759A1

公开(公告)日：2015-03-05

申请号：US14478737

申请日：2014-09-05

Applicant: Braskem S.A.

Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

IPC: C12P7/18 ,  C12P7/04

CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of cytosolic acetyl-CoA to 2-propanol; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of 2-propanol and 1-propanol and/or 1,2-propanediol.

Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 一种或多种编码催化细胞溶质乙酰辅酶A转化为2-丙醇的途径中的一种或多种酶的多核苷酸; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产2-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。

公开(公告)号：US20180346936A1

公开(公告)日：2018-12-06

申请号：US16042594

申请日：2018-07-23

Applicant: Braskem S.A.

Inventor： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC: C12P5/02 ,  C12N9/88 ,  C12N15/52

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y402/01127

Abstract: The present disclosure generally relates to methods of using microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene and products and processes derived therefrom.