公开(公告)号：US20240425632A1

公开(公告)日：2024-12-26

申请号：US18762973

申请日：2024-07-03

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Randall Smith ,  Wayne George ,  Andrew Brown

IPC: C08F222/40 ,  C07H21/00 ,  C08F283/12 ,  C08J7/12 ,  C12N15/10

Abstract: Some examples herein provide a method that includes reacting an unsaturated cyclic dione with an indole or indazole to form a first adduct. One of a substrate and a functional group is coupled to the unsaturated cyclic dione, and the other of the substrate and the functional group is coupled to the indole or indazole. Forming the first adduct couples the functional group to the substrate. Some examples herein provide a composition that includes a substate, a functional group, and an adduct coupling the functional group to the substrate. The adduct may be a product of a reaction between an unsaturated cyclic dione and an indole or indazole.

公开(公告)号：US20210139978A1

公开(公告)日：2021-05-13

申请号：US17153309

申请日：2021-01-20

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US20190264279A1

公开(公告)日：2019-08-29

申请号：US16405679

申请日：2019-05-07

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US20250010285A1

公开(公告)日：2025-01-09

申请号：US18750789

申请日：2024-06-21

Applicant: ILLUMINA, INC.

Inventor： Wayne N. George ,  Iuliana Petruta Maria ,  Randall Smith ,  Andrew A. Brown

IPC: B01L3/00 ,  C07D257/08 ,  C07F9/6561

Abstract: An example of a flow cell includes a substrate including a surface and a dendron architecture. The dendron architecture includes a functionalized focal point of attachment that is attached to the substrate surface and a plurality of peripheral functional groups that are orthogonal to the functionalized focal point of attachment. The flow cell further includes a primer set attached to the dendron architecture via the plurality of peripheral functional groups.

公开(公告)号：US20240141428A1

公开(公告)日：2024-05-02

申请号：US18489788

申请日：2023-10-18

Applicant: Illumina, Inc.

Inventor： Robert C. Kain ,  Xiaohai Liu ,  Wenyi Feng ,  Bernard Hirschbein ,  Helmy A. Eltoukhy ,  Xiaolin Wu ,  Geoffrey Paul Smith ,  Jonathan Mark Boutell ,  Thomas Joseph ,  Randall Smith ,  Min-Jui Richard Shen ,  Carolyn Tregidgo ,  Kay Klausing

IPC: C12Q1/6874 ,  C12Q1/6869

CPC classification number: C12Q1/6874 ,  C12Q1/6869

Abstract: The present disclosure provides methods and systems for detecting multiple different nucleotides in a sample. In particular, the disclosure provides for detection of multiple different nucleotides in a sample utilizing fewer detection moieties than the number of nucleotides being detected and/or fewer imaging events than the number of nucleotides being detected.

公开(公告)号：US11952619B2

公开(公告)日：2024-04-09

申请号：US16868308

申请日：2020-05-06

Applicant: ILLUMINA, INC.

Inventor： Peyton Shieh ,  John M. Beierle ,  Michael S. Graige ,  Alexander Fuhrmann ,  Randall Smith ,  Wei Wei ,  Naiqian Zhan

IPC: C12Q1/6837

CPC classification number: C12Q1/6837 ,  C12Q1/6837 ,  C12Q2565/507

Abstract: An array includes a support including a plurality of discrete wells, a gel material positioned in each of the plurality of discrete wells, and a quality control tracer grafted to the gel material in each of the plurality of discrete wells. The quality control tracer comprises (a) a cleavable nucleotide sequence comprising a cleavage site and (b) a detectable label; and in some aspects, is a cleavable nucleotide sequence with a detectable label and a non-reactive nucleotide sequence or a primer nucleotide sequence.

公开(公告)号：US20220250066A1

公开(公告)日：2022-08-11

申请号：US17727622

申请日：2022-04-22

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US12123833B2

公开(公告)日：2024-10-22

申请号：US18512284

申请日：2023-11-17

Applicant: Illumina, Inc.

Inventor： Tracy H. Fung ,  Poorya Sabounchi ,  Bernard Hirschbein ,  Joseph Pinto ,  Tarun Khurana ,  Randall Smith ,  Wenyi Feng

IPC: G01N21/64 ,  G01N33/53

CPC classification number: G01N21/6428 ,  G01N21/6454 ,  G01N33/53 ,  G01N2021/6471 ,  G01N2201/08

Abstract: There is set forth herein a device comprising structure defining a detector surface configured for supporting biological or chemical substances, and a sensor array comprising light sensors and circuitry to transmit data signals using photons detected by the light sensors. The device can include one or more features for reducing fluorescence range noise in a detection band of the sensor array.

公开(公告)号：US12083514B2

公开(公告)日：2024-09-10

申请号：US17727622

申请日：2022-04-22

Applicant: ILLUMINA, Inc.

Inventor： Maxwell Zimmerley ,  LiangLiang Qiang ,  M. Shane Bowen ,  Steven H. Modiano ,  Dajun Yuan ,  Randall Smith ,  Arthur J. Pitera ,  Hai Quang Tran ,  Gerald Kreindl

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502746

Abstract: An interposer for a flow cell comprises a base layer having a first surface and a second surface opposite the first surface. The base layer comprises black polyethylene terephthalate (PET). A first adhesive layer is disposed on the first surface of the base layer. The first adhesive layer comprises methyl acrylic adhesive. A second adhesive layer is disposed on the second surface of the base layer. The second adhesive layer comprises methyl acrylic adhesive. A plurality of microfluidic channels extends through each of the base layer, the first adhesive layer, and the second adhesive layer.

公开(公告)号：US12060448B2

公开(公告)日：2024-08-13

申请号：US17565818

申请日：2021-12-30

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Randall Smith ,  Wayne George ,  Andrew Brown

IPC: C08J7/12 ,  C07H21/00 ,  C08F222/40 ,  C08F283/12 ,  C12N15/10

CPC classification number: C08F222/40 ,  C07H21/00 ,  C08F283/12 ,  C08J7/12 ,  C12N15/1006 ,  C12N2310/51

Abstract: In one example, an unsaturated cyclic dione is coupled to the substrate, and is reacted with an indole or indazole including a first functional group to form a first adduct coupling the first functional group to the substrate. In another example, an unsaturated cyclic dione is coupled to a substrate and reacted with a diene including a functional group to form an adduct coupling the functional group to the substrate. In another example, an indole or indazole is coupled to a substrate, and is reacted with an unsaturated cyclic dione including an oligonucleotide to form an adduct coupling the oligonucleotide to the substrate. In another example, a diene is coupled to a substrate, and is reacted with an unsaturated cyclic dione including an oligonucleotide to form an adduct coupling the oligonucleotide to the substrate.