-
1.发明授权公开(公告)号：US08119984B2
公开(公告)日：2012-02-21
申请号：US12473570
申请日：2009-05-28
申请人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, Jr. , Donald F. Hunt , Christopher Mullen
发明人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, Jr. , Donald F. Hunt , Christopher Mullen
IPC分类号： H01J49/10
CPC分类号： H01J49/12 , H01J49/0072
摘要： A front-end reagent ion source for a mass spectrometer is disclosed. Reagent vapor is supplied to a reagent ionization volume located within a chamber of the mass spectrometer and maintained at a low vacuum pressure. Reagent ions are formed by interaction of the reagent vapor molecules with an electrical discharge (e.g., a glow discharge) within the ionization volume, and pass into the chamber of the mass spectrometer. At least one ion optical element located along the analyte ion path transports the reagent ions to successive chambers of the mass spectrometer. The reagent ions may be combined with the analyte ions to perform ion-ion studies such as electron transfer dissociation (ETD).
摘要翻译： 公开了用于质谱仪的前端试剂离子源。 将试剂蒸气供给到位于质谱仪室内的试剂电离体并保持在低真空压力下。 试剂离子通过试剂蒸气分子与离子化体积内的放电（例如，辉光放电）的相互作用而形成，并进入质谱仪的室。 沿着分析物离子路径定位的至少一个离子光学元件将试剂离子输送到质谱仪的连续室。 试剂离子可与分析物离子组合以进行离子离子研究，例如电子转移离解（ETD）。
-
2.发明申请公开(公告)号：US20090294649A1
公开(公告)日：2009-12-03
申请号：US12473570
申请日：2009-05-28
申请人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, JR. , Donald F. Hunt , Christopher Mullen
发明人： Jeffrey Shabanowitz , Philip D. Compton , Lee Earley , George C. Stafford, JR. , Donald F. Hunt , Christopher Mullen
CPC分类号： H01J49/12 , H01J49/0072
摘要： A front-end reagent ion source for a mass spectrometer is disclosed. Reagent vapor is supplied to a reagent ionization volume located within a chamber of the mass spectrometer and maintained at a low vacuum pressure. Reagent ions are formed by interaction of the reagent vapor molecules with an electrical discharge (e.g., a glow discharge) within the ionization volume, and pass into the chamber of the mass spectrometer. At least one ion optical element located along the analyte ion path transports the reagent ions to successive chambers of the mass spectrometer. The reagent ions may be combined with the analyte ions to perform ion-ion studies such as electron transfer dissociation (ETD).
摘要翻译： 公开了用于质谱仪的前端试剂离子源。 将试剂蒸气供给到位于质谱仪室内的试剂电离体并保持在低真空压力下。 试剂离子通过试剂蒸气分子与离子化体积内的放电（例如，辉光放电）的相互作用而形成，并进入质谱仪的室。 沿着分析物离子路径定位的至少一个离子光学元件将试剂离子输送到质谱仪的连续室。 试剂离子可与分析物离子组合以进行离子离子研究，例如电子转移离解（ETD）。
-
公开(公告)号：US4066894A
公开(公告)日：1978-01-03
申请号：US650783
申请日：1976-01-20
CPC分类号： H01J49/4215 , H01J49/0095 , H01J49/025
摘要： A method and an apparatus are disclosed for adapting a conventional quadrupole mass spectrometer to substantially simultaneously produce and record both positive and negative ions. The apparatus includes a control circuit for rapidly switching the repeller, source and lens electrodes of a quadrupole mass spectrometer between positive and negative potentials. This switching of the potentials, along with the selection of appropriately favorable ionization conditions, permits the generation of suitable streams of positive and negative ions. A dual electron multiplier detector is used for separately sensing the positive and negative ions transmitted through the quadrupole mass spectrometer. The disclosed method and apparatus are particularly suitable for obtaining accurate mass measurements using a quadrupole mass spectrometer.
摘要翻译： 公开了用于使常规四极质谱仪适应于基本上同时产生和记录正离子和负离子的方法和装置。 该装置包括用于在正电位和负电位之间快速切换四极质谱仪的反射器，源极和透镜电极的控制电路。 这种电位的切换以及合适的电离条件的选择允许产生合适的正离子和负离子流。 双电子倍增器检测器用于单独检测通过四极杆质谱仪传输的正离子和负离子。 所公开的方法和装置特别适用于使用四极质谱仪获得准确的质量测量。
-
公开(公告)号：US4136280A
公开(公告)日：1979-01-23
申请号：US795148
申请日：1977-05-09
CPC分类号： H01J49/4215 , H01J49/0095 , H01J49/025
摘要： A method and an apparatus are disclosed for adapting a conventional quadrupole mass spectrometer to substantially simultaneously produce and record both positive and negative ions. The apparatus includes a control circuit for rapidly switching the repeller, source and lens electrodes of a quadrupole mass spectrometer between positive and negative potentials. This switching of the potentials, along with the selection of appropriately favorable ionization conditions, permits the generation of suitable streams of positive and negative ions. A dual electron multiplier detector is used for separately sensing the positive and negative ions transmitted through the quadrupole mass spectrometer. The disclosed method and apparatus are particularly suitable for obtaining accurate mass measurements using a quadrupole mass spectrometer.
-
5.发明授权
公开(公告)号：US4175234A
公开(公告)日：1979-11-20
申请号：US916151
申请日：1978-06-16
CPC分类号： H01J49/145 , H01J49/16
摘要： An apparatus for ionizing and vaporizing non-volatile or thermally labile molecules of a sample for introduction into a mass spectrometer analyzer which comprises a housing containing an ionization chamber; an ion exit port for the introduction of gaseous ions from said ionization chamber into a mass spectrometer analyzer on the portion of the housing attached to said mass spectrometer analyzer; inlet means for introducing a gaseous reactant into said ionization chamber within said housing; inlet means for admitting high energy radiation into said ionization chamber within said housing to at least partially ionize the molecules of said gaseous reactant; aperture means open into said ionization chamber of said housing for admitting a conductive emitter characterized by having a conductive element of a highly irregular surface upon which is deposited said sample for analysis into said mass spectrometer analyzer; and a vacuum exit aperture in said housing by which the pressure within said ionization chamber can be reduced.
摘要翻译： 一种用于离子化和蒸发样品的非挥发性或热不稳定分子的装置，用于引入质谱仪分析仪，其包括含有电离室的壳体; 离子出口，用于将气态离子从所述电离室引入质谱仪分析仪，所述质谱仪分析仪附着在所述质谱仪分析仪上; 用于将气体反应物引入所述壳体内的所述电离室的入口装置; 入口装置，用于将高能量辐射进入所述壳体内的所述电离室，以使所述气态反应物的分子至少部分电离; 开口意味着通向所述壳体的所述电离室，用于允许导电发射器，其特征在于具有高度不规则表面的导电元件，在其上沉积所述样品用于分析所述质谱仪分析仪; 以及所述壳体中的真空出口孔，通过该真空出口孔可以减小所述电离室内的压力。
-
公开(公告)号：US4156814A
公开(公告)日：1979-05-29
申请号：US822216
申请日：1977-08-05
CPC分类号： H01J49/0409 , H01J49/142
摘要： An apparatus for ionizing and vaporizing non-volatile or thermally labile molecules of a sample for introduction into a mass spectrometer analyzer which comprises a housing containing an ionization chamber; an ion exit port for the introduction of gaseous ions from said ionization chamber into a mass spectrometer analyzer on the portion of the housing attached to said mass spectrometer analyzer; inlet means for introducing a gaseous reactant into said ionization chamber within said housing; inlet means for admitting high energy radiation into said ionization chamber within said housing to at least partially ionize the molecules of said gaseous reactant; aperture means open into said ionization chamber of said housing for admitting a conductive emitter characterized by having a conductive element of a highly irregular surface upon which is deposited said sample for analysis into said mass spectrometer analyzer; and a vacuum exit aperture in said housing by which the pressure within said ionization chamber can be reduced.
-
公开(公告)号：US11538675B2
公开(公告)日：2022-12-27
申请号：US16307615
申请日：2017-06-05
申请人： Donald F. Hunt , Ann M. English , Scott A. Ugrin , Dina L. Bai , Jeffrey Shabanowitz , John Edward Philip Syka
发明人： Donald F. Hunt , Ann M. English , Scott A. Ugrin , Dina L. Bai , Jeffrey Shabanowitz , John Edward Philip Syka
摘要： The present disclosure relates to novel and improved methods of analyzing proteins, peptides and polypeptides by mass spectrometry using ion-ion reactions. More specifically the disclosure relates to improved methods for implementing the m/z selective arresting of ion-ion reactions within the ion-ion reaction cell of a mass spectrometer system during a period where ion-ion reactions are performed.
-
8.发明申请公开(公告)号：US20130259883A1
公开(公告)日：2013-10-03
申请号：US13699563
申请日：2011-05-24
申请人： Donald F. Hunt , Jeffrey Shabanowitz , Jennifer Cottine , Ann M. English , Andrew Norris , Victor H. Engelhard , Mark Cobbold , Kara L. Cummings , Angela Zarling , Rebecca C. Obeng , Jie Qian
发明人： Donald F. Hunt , Jeffrey Shabanowitz , Jennifer Cottine , Ann M. English , Andrew Norris , Victor H. Engelhard , Mark Cobbold , Kara L. Cummings , Angela Zarling , Rebecca C. Obeng , Jie Qian
CPC分类号： C07K7/06 , A61K38/00 , A61K39/0011 , C07K7/08 , C07K14/70539 , C07K16/18 , G01N33/5743 , G01N33/57492 , G01N33/6893 , G01N2333/70539 , G01N2440/14
摘要： A set of phosphorylated peptides are presented by HLA A*0101, A*0201, A*0301, B*4402, B*2705, B*1402, and B*0702 on the surface of melanoma cells. They have the potential to (a) stimulate an immune response to the cancer, (b) to function as immunotherapeutics in adoptive T-cell therapy or as a vaccine, (c) to facilitate antibody recognition of the tumor boundaries in surgical pathology samples, and (d) act as biomarkers for early detection of the disease. Phosphorylated peptides are also presented for other cancers.
摘要翻译： 一组磷酸化肽由黑素瘤细胞表面上的HLA A * 0101，A * 0201，A * 0301，B * 4402，B * 2705，B * 1402和B * 0702呈现。 它们有可能（a）刺激对癌症的免疫应答，（b）在过继性T细胞治疗或疫苗中起到免疫治疗的作用，（c）促进外科病理样本中肿瘤边界的抗体识别， 和（d）作为早期检测疾病的生物标志物。 磷酸化肽也可用于其他癌症。
-
9.发明授权Peptides recognized by melanoma-specific A1-, A2- and A3-restricted cytoxic lymphocytes, and uses therefor 失效由黑素瘤特异性A1-，A2-和A3-限制性细胞毒性淋巴细胞识别的肽及其用途公开(公告)号：US07019112B1
公开(公告)日：2006-03-28
申请号：US08816454
申请日：1997-03-12
申请人： Craig L. Slingluff , Donald F. Hunt , Jeffrey Shabanowitz , Andrea L. Cox , Victor H. Engelhard , David Kittlesen , Jonathan Skipper , Ronald C. Hendrikson
发明人： Craig L. Slingluff , Donald F. Hunt , Jeffrey Shabanowitz , Andrea L. Cox , Victor H. Engelhard , David Kittlesen , Jonathan Skipper , Ronald C. Hendrikson
IPC分类号： C07K7/06
摘要： Melanoma-specific, A1- and A3-restricted CTL epitopes have been identified in tyrosinase and pMel-17, respectively, and may be used in the design of vaccines.
摘要翻译： 已经在酪氨酸酶和pMel-17中鉴定了黑素瘤特异性，A1-和A3限制性CTL表位，并且可以用于疫苗的设计。
-
公开(公告)号：US09171707B2
公开(公告)日：2015-10-27
申请号：US13391331
申请日：2010-09-02
CPC分类号： H01J49/0072 , Y10T436/24
摘要： The invention provides improvements in reagents for use in electron transfer dissociation ionization techniques for use in mass spectrometry, particularly for sequencing peptides and proteins using mass spectrometric techniques involving electrospray ionization and MS/MS characterization of fragment ions. The novel reagents used in the inventive methods allow for more effective determination of protein sequences, especially of long peptides or post-translationally modified protein fragments. Use of the polycyclic aromatic hydrocarbons azulene, homoazulene, and acenaphthylene, and homodimers and heterodimers thereof, are described.
摘要翻译： 本发明提供了用于质谱法中使用的电子转移离解电离技术的试剂的改进，特别是使用涉及电喷雾离子化和片段离子的MS / MS表征的质谱技术测序肽和蛋白质。 用于本发明方法的新颖试剂允许更有效地测定蛋白质序列，特别是长肽或翻译后修饰的蛋白质片段。 描述了多环芳香烃薁，高ene烯和苊烯及其同二聚体和异二聚体的用途。
-
-
-
-
-
-
-
-
-
搜索结果
31 条记录 (耗时 0.043 秒)
