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31 条记录 (耗时 0.033 秒)

    Reagents for electron transfer dissociation in mass spectrometry analysis
    1.
    发明授权
    Reagents for electron transfer dissociation in mass spectrometry analysis 有权
    质谱分析中电子转移离解试剂

    公开(公告)号：US09171707B2

    公开(公告)日：2015-10-27

    申请号：US13391331

    申请日：2010-09-02

    申请人： John E. P. Syka ,  Philip D. Compton ,  Donald F. Hunt

    发明人： John E. P. Syka ,  Philip D. Compton ,  Donald F. Hunt

    IPC分类号： G01N24/00 ,  H01J49/00

    CPC分类号： H01J49/0072 ,  Y10T436/24

    摘要： The invention provides improvements in reagents for use in electron transfer dissociation ionization techniques for use in mass spectrometry, particularly for sequencing peptides and proteins using mass spectrometric techniques involving electrospray ionization and MS/MS characterization of fragment ions. The novel reagents used in the inventive methods allow for more effective determination of protein sequences, especially of long peptides or post-translationally modified protein fragments. Use of the polycyclic aromatic hydrocarbons azulene, homoazulene, and acenaphthylene, and homodimers and heterodimers thereof, are described.

    摘要翻译： 本发明提供了用于质谱法中使用的电子转移离解电离技术的试剂的改进，特别是使用涉及电喷雾离子化和片段离子的MS / MS表征的质谱技术测序肽和蛋白质。 用于本发明方法的新颖试剂允许更有效地测定蛋白质序列，特别是长肽或翻译后修饰的蛋白质片段。 描述了多环芳香烃薁，高ene烯和苊烯及其同二聚体和异二聚体的用途。

    Method and Apparatus for Generation of Reagent Ions in a Mass Spectrometer
    2.
    发明申请
    Method and Apparatus for Generation of Reagent Ions in a Mass Spectrometer 有权
    用于在质谱仪中产生试剂离子的方法和装置

    公开(公告)号：US20090294649A1

    公开(公告)日：2009-12-03

    申请号：US12473570

    申请日：2009-05-28

    申请人： Jeffrey Shabanowitz ,  Philip D. Compton ,  Lee Earley ,  George C. Stafford, JR. ,  Donald F. Hunt ,  Christopher Mullen

    发明人： Jeffrey Shabanowitz ,  Philip D. Compton ,  Lee Earley ,  George C. Stafford, JR. ,  Donald F. Hunt ,  Christopher Mullen

    IPC分类号： B01D59/44 ,  H01J49/00 ,  H01J27/00

    CPC分类号： H01J49/12 ,  H01J49/0072

    摘要： A front-end reagent ion source for a mass spectrometer is disclosed. Reagent vapor is supplied to a reagent ionization volume located within a chamber of the mass spectrometer and maintained at a low vacuum pressure. Reagent ions are formed by interaction of the reagent vapor molecules with an electrical discharge (e.g., a glow discharge) within the ionization volume, and pass into the chamber of the mass spectrometer. At least one ion optical element located along the analyte ion path transports the reagent ions to successive chambers of the mass spectrometer. The reagent ions may be combined with the analyte ions to perform ion-ion studies such as electron transfer dissociation (ETD).

    摘要翻译： 公开了用于质谱仪的前端试剂离子源。 将试剂蒸气供给到位于质谱仪室内的试剂电离体并保持在低真空压力下。 试剂离子通过试剂蒸气分子与离子化体积内的放电（例如，辉光放电）的相互作用而形成，并进入质谱仪的室。 沿着分析物离子路径定位的至少一个离子光学元件将试剂离子输送到质谱仪的连续室。 试剂离子可与分析物离子组合以进行离子离子研究，例如电子转移离解（ETD）。

    Method and apparatus for generation of reagent ions in a mass spectrometer
    3.
    发明授权
    Method and apparatus for generation of reagent ions in a mass spectrometer 有权
    用于在质谱仪中产生试剂离子的方法和装置

    公开(公告)号：US08119984B2

    公开(公告)日：2012-02-21

    申请号：US12473570

    申请日：2009-05-28

    申请人： Jeffrey Shabanowitz ,  Philip D. Compton ,  Lee Earley ,  George C. Stafford, Jr. ,  Donald F. Hunt ,  Christopher Mullen

    发明人： Jeffrey Shabanowitz ,  Philip D. Compton ,  Lee Earley ,  George C. Stafford, Jr. ,  Donald F. Hunt ,  Christopher Mullen

    IPC分类号： H01J49/10

    CPC分类号： H01J49/12 ,  H01J49/0072

    摘要： A front-end reagent ion source for a mass spectrometer is disclosed. Reagent vapor is supplied to a reagent ionization volume located within a chamber of the mass spectrometer and maintained at a low vacuum pressure. Reagent ions are formed by interaction of the reagent vapor molecules with an electrical discharge (e.g., a glow discharge) within the ionization volume, and pass into the chamber of the mass spectrometer. At least one ion optical element located along the analyte ion path transports the reagent ions to successive chambers of the mass spectrometer. The reagent ions may be combined with the analyte ions to perform ion-ion studies such as electron transfer dissociation (ETD).

    摘要翻译： 公开了用于质谱仪的前端试剂离子源。 将试剂蒸气供给到位于质谱仪室内的试剂电离体并保持在低真空压力下。 试剂离子通过试剂蒸气分子与离子化体积内的放电（例如，辉光放电）的相互作用而形成，并进入质谱仪的室。 沿着分析物离子路径定位的至少一个离子光学元件将试剂离子输送到质谱仪的连续室。 试剂离子可与分析物离子组合以进行离子离子研究，例如电子转移离解（ETD）。

    REAGENTS FOR ELECTRON TRANSFER DISSOCIATION IN MASS SPECTROMETRY ANALYSIS
    4.
    发明申请
    REAGENTS FOR ELECTRON TRANSFER DISSOCIATION IN MASS SPECTROMETRY ANALYSIS 有权
    大量光谱分析中电子转移分离的试剂

    公开(公告)号：US20120156792A1

    公开(公告)日：2012-06-21

    申请号：US13391331

    申请日：2010-09-02

    申请人： John E.P. Syka ,  Philip D. Compton ,  Donald F. Hunt

    发明人： John E.P. Syka ,  Philip D. Compton ,  Donald F. Hunt

    IPC分类号： G01N27/62

    CPC分类号： H01J49/0072 ,  Y10T436/24

    摘要： The invention provides improvements in reagents for use in electron transfer dissociation ionization techniques for use in mass spectrometry, particularly for sequencing peptides and proteins using mass spectrometric techniques involving electro-spray ionization and MS/MS characterization of fragment ions. The novel reagents used in the inventive methods allow for more effective determination of protein sequences, especially of long peptides or post-translationally modified protein fragments. Use of the polycyclic aromatic hydrocarbons azulene, homoazulene, and acenaphthylene, and homodimers and heterodimers thereof, are described.

    摘要翻译： 本发明提供了用于电子转移离解电离技术用于质谱法的试剂的改进，特别是使用涉及电喷雾电离和片段离子的MS / MS表征的质谱技术测序肽和蛋白质。 用于本发明方法的新颖试剂允许更有效地测定蛋白质序列，特别是长肽或翻译后修饰的蛋白质片段。 描述了多环芳香烃薁，高ene烯和苊烯及其同二聚体和异二聚体的用途。

    Compositions and methods for analysis of protein sequences and post-translational modifications
    5.
    发明授权
    Compositions and methods for analysis of protein sequences and post-translational modifications 有权

    公开(公告)号：US10281473B2

    公开(公告)日：2019-05-07

    申请号：US15116609

    申请日：2015-02-04

    申请人： University of Virginia Patent Foundation ,  Donald F. Hunt ,  Weihan Wang ,  Lichao Zhang

    发明人： Donald F. Hunt ,  Weihan Wang ,  Lichao Zhang

    IPC分类号： G01N33/68

    摘要： The application discloses compositions, methods, systems, and apparatuses for rapid sequence analysis of proteins, including location of post-translational modifications and disulfide bonds. Limited digestion of fully denatured antibody occurs in seconds by flowing sample in 8M urea at constant pressure through a micro column reactor containing immobilized aspergillopepsin I, resulting in a product mixture containing 3-10 kDa peptides, which is then fractionated by capillary column chromatography and analyzed by both electron transfer dissociation (ETD) and collision activated dissociation mass spectrometry. This method provides 95% sequence coverage of a mAb and detects numerous post-translational modifications. For disulfide bond location, native mAb is subjected to longer digestion times. Release of disulfide containing peptides from accessible regions of the folded protein occurs with short digestion times. The identity of peptides connected by a disulfide bond is determined using ETD and ion-ion proton transfer chemistry.