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1.发明申请Process for producing microbial fat or oil having lowered unsaponifiable matter content and said fat or oil 有权用于生产具有降低的不皂化物质含量和所述脂肪或油的微生物脂肪或油的方法公开(公告)号：US20050287651A1
公开(公告)日：2005-12-29
申请号：US10530747
申请日：2003-10-09
CPC分类号： C12P7/6472 , A23D9/02
摘要： A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid is cultured in a medium containing a nitrogen source concentration of 2 to 15% within a culture tank equipped with an agitation impeller satisfying the requirement that the ratio of the diameter of agitation impeller (=d) to the inner diameter of the culture tank (=D) is d/D=0.30 to 0.6.
摘要翻译： 一种生产具有降低的不皂化物质含量和/或酯类固醇含量并含有高度不饱和脂肪酸作为构成脂肪酸的原油的方法，其特征在于，能够生产包含不饱和脂肪酸的脂肪或油的微生物 作为构成脂肪酸，在含有浓度为2〜15％的氮源的培养基中培养，在配备有搅拌叶轮的培养槽中，搅拌叶轮满足搅拌叶轮直径（= d）与内径 培养罐（= D）为d / D = 0.30〜0.6。
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2.发明授权Process for producing microbial fat or oil having lowered unsaponifiable matter content and said fat or oil 有权用于生产具有降低的不皂化物质含量和所述脂肪或油的微生物脂肪或油的方法公开(公告)号：US07709236B2
公开(公告)日：2010-05-04
申请号：US10530747
申请日：2003-10-09
IPC分类号： C12P7/64
CPC分类号： C12P7/6472 , A23D9/02
摘要： A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid is cultured in a medium containing a nitrogen source concentration of 2 to 15% within a culture tank equipped with an agitation impeller satisfying the requirement that the ratio of the diameter of agitation impeller (=d) to the inner diameter of the culture tank (=D) is d/D=0.30 to 0.6.
摘要翻译： 一种生产具有降低的不皂化物质含量和/或酯类固醇含量并含有高度不饱和脂肪酸作为构成脂肪酸的原油的方法，其特征在于，能够生产包含不饱和脂肪酸的脂肪或油的微生物 作为构成脂肪酸，在含有浓度为2〜15％的氮源的培养基中培养，在配备有搅拌叶轮的培养槽中，搅拌叶轮满足搅拌叶轮直径（= d）与内径 培养罐（= D）为d / D = 0.30〜0.6。
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3.发明授权Production method of oil or fat containing polyunsaturated fatty acid-containing triglyceride 有权含有多不饱和脂肪酸的甘油三酸酯的油脂的生产方法公开(公告)号：US07538238B2
公开(公告)日：2009-05-26
申请号：US10482373
申请日：2002-07-02
IPC分类号： A23D9/00
CPC分类号： C12P7/6472 , A23C9/1528 , A23C11/04 , A23K20/158 , A23L33/115 , A23L33/12 , C11C3/08 , C11C3/10
摘要： The present invention relates to a production method of an oil containing triglyceride in which medium chain fatty acids are bound to the 1- and 3-positions of the triglyceride and polyunsaturated fatty acid is bound to the 2 position by allowing lipase, which specifically acts on ester bonds at the 1- and 3-positions that has been immobilized on a porous ion exchange resin support having a pore size of about 100 Angstroms or more, to act on a mixture of medium-chain fatty acids and raw material oil containing at least one polyunsaturated fatty acid selected from the group consisting of ω6 series polyunsaturated fatty acid having 18 or more carbon atoms and 3 or more double bonds and ω9 series polyunsaturated fatty acid having 18 or more carbon atoms and 2 or more double bonds, but not containing ω3 series polyunsaturated fatty acid, oils and fats or triglycerides obtained by that method, and the use of the oils and fats or triglycerides in a food, beverage or pharmaceutical composition.
摘要翻译： 本发明涉及含有甘油三酯的油的制备方法，其中中链脂肪酸与甘油三酯的1位和3位结合，多不饱和脂肪酸通过使脂肪酶特异性地作用于2位而结合到2位上 在固定在孔径为约100埃以上的多孔离子交换树脂载体上的1-位和3-位的酯键作用于中链脂肪酸和至少含有至少 一种选自具有18个或更多个碳原子的ω-6系多不饱和脂肪酸和3个或更多个双键的多不饱和脂肪酸和具有18个或更多个碳原子和2个或更多个双键但不含ω3的ω-系列多不饱和脂肪酸 系列多不饱和脂肪酸，通过该方法获得的油和脂肪或甘油三酯，以及在食品，饮料或药物中使用油和脂肪或甘油三酸酯 l组成。
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4.发明授权Production method of oil or fat containing polyunsaturated fatty acid-containing triglyceride 有权含有多不饱和脂肪酸的甘油三酸酯的油脂的生产方法公开(公告)号：US07767427B2
公开(公告)日：2010-08-03
申请号：US12232851
申请日：2008-09-25
IPC分类号： C12P7/64
CPC分类号： C12P7/6472 , A23C9/1528 , A23C11/04 , A23K20/158 , A23L33/115 , A23L33/12 , C11C3/08 , C11C3/10
摘要： The present invention relates to a production method of an oil containing triglyceride in which medium chain fatty acids are bound to the 1- and 3-positions of the triglyceride and polyunsaturated fatty acid is bound to the 2 position by allowing lipase, which specifically acts on ester bonds at the 1- and 3-positions that has been immobilized on a porous ion exchange resin support having a pore size of about 100 Angstroms or more, to act on a mixture of medium-chain fatty acids and raw material oil containing at least one polyunsaturated fatty acid selected from the group consisting of ω6 series polyunsaturated fatty acid having 18 or more carbon atoms and 3 or more double bonds and ω9 series polyunsaturated fatty acid having 18 or more carbon atoms and 2 or more double bonds, but not containing ω3 series polyunsaturated fatty acid, oils and fats or triglycerides obtained by that method, and the use of the oils and fats or triglycerides in a food, beverage or pharmaceutical composition.
摘要翻译： 本发明涉及含有甘油三酯的油的制备方法，其中中链脂肪酸与甘油三酯的1位和3位结合，多不饱和脂肪酸通过使脂肪酶特异性地作用于2位而结合到2位上 在已经固定在孔径为约100埃以上的多孔离子交换树脂载体上的1-位和3-位的酯键作用于至少含有至少含有中链脂肪酸和原料油的混合物 一种选自具有18个或更多个碳原子的ω6系多不饱和脂肪酸和3个或更多个双键的多不饱和脂肪酸和具有18个以上碳原子和2个以上双键但不含ω3的ω9系多不饱和脂肪酸 系列多不饱和脂肪酸，通过该方法获得的油和脂肪或甘油三酯，以及在食品，饮料或药物组合物中使用油和脂肪或甘油三酸酯 。
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5.发明申请公开(公告)号：US20090076149A1
公开(公告)日：2009-03-19
申请号：US12232851
申请日：2008-09-25
CPC分类号： C12P7/6472 , A23C9/1528 , A23C11/04 , A23K20/158 , A23L33/115 , A23L33/12 , C11C3/08 , C11C3/10
摘要： The present invention relates to a production method of an oil containing triglyceride in which medium chain fatty acids are bound to the 1- and 3-positions of the triglyceride and polyunsaturated fatty acid is bound to the 2 position by allowing lipase, which specifically acts on ester bonds at the 1- and 3-positions that has been immobilized on a porous ion exchange resin support having a pore size of about 100 Angstroms or more, to act on a mixture of medium-chain fatty acids and raw material oil containing at least one polyunsaturated fatty acid selected from the group consisting of ω6 series polyunsaturated fatty acid having 18 or more carbon atoms and 3 or more double bonds and ω9 series polyunsaturated fatty acid having 18 or more carbon atoms and 2 or more double bonds, but not containing ω3 series polyunsaturated fatty acid, oils and fats or triglycerides obtained by that method, and the use of the oils and fats or triglycerides in a food, beverage or pharmaceutical composition.
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公开(公告)号：US20060141592A1
公开(公告)日：2006-06-29
申请号：US10527703
申请日：2003-09-12
申请人： Motoo Sumida , Kenichi Higashiyama
发明人： Motoo Sumida , Kenichi Higashiyama
IPC分类号： C12P7/64
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/115 , A23V2002/00 , C11C3/10 , A23V2250/1862
摘要： There are provided polyunsaturated fatty acids-containing oils/fats or triglycerides with high digestion and absorption properties and resistance to oxidative damage, which are suitable for applications in fields such as modified milk for infants, food products and healthy foods and/or supplements, produced by transesterification of polyunsaturated fatty acids-containing oils/fats or triglycerides with vegetable oils/fats or triglycerides using 1,3-position specific type lipases.
摘要翻译： 提供了含有不饱和脂肪酸的油/脂肪或甘油三酸酯，具有高消化吸收性能和抗氧化损伤性，适用于婴幼儿改良牛奶，食品和健康食品和/或补品等领域。 通过使用1,3位特异性脂肪酶将多不饱和脂肪酸的油/脂肪或甘油三酯与植物油/脂肪或甘油三酸酯进行酯交换。
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公开(公告)号：US07998712B2
公开(公告)日：2011-08-16
申请号：US10527703
申请日：2003-09-12
申请人： Motoo Sumida , Kenichi Higashiyama
发明人： Motoo Sumida , Kenichi Higashiyama
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/115 , A23V2002/00 , C11C3/10 , A23V2250/1862
摘要： There are provided polyunsaturated fatty acids-containing oils/fats or triglycerides with high digestion and absorption properties and resistance to oxidative damage, which are suitable for applications in fields such as modified milk for infants, food products and healthy foods and/or supplements, produced by transesterification of polyunsaturated fatty acids-containing oils/fats or triglycerides with vegetable oils/fats or triglycerides using 1,3-position specific type lipases.
摘要翻译： 提供了含有不饱和脂肪酸的油/脂肪或甘油三酸酯，具有高消化吸收性能和抗氧化损伤性，适用于婴幼儿改良牛奶，食品和健康食品和/或补品等领域。 通过使用1,3位特异性脂肪酶将多不饱和脂肪酸的油/脂肪或甘油三酯与植物油/脂肪或甘油三酸酯进行酯交换。
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8.发明授权Producing method of phospholipids including long-chain polyunsaturated fatty acids as constituents, and use of such phospholipids 有权包括长链多不饱和脂肪酸作为成分的磷脂的生产方法，以及使用这种磷脂公开(公告)号：US07968737B2
公开(公告)日：2011-06-28
申请号：US12850389
申请日：2010-08-04
申请人： Hiroshi Kawashima , Motoo Sumida , Akiko Shiraishi
发明人： Hiroshi Kawashima , Motoo Sumida , Akiko Shiraishi
IPC分类号： C07F9/02
CPC分类号： C12P9/00 , A23J7/00 , A23L33/115 , C07F9/103 , C12P7/6481 , C12P13/06
摘要： The present invention provides a method of efficiently and stably producing LCPUFA-PL. Specifically, the invention provides a method for producing phospholipids that contain LCPUFA as a constituent (LCPUFA-PL), wherein lipid producing cells producing lipids that contain long-chain polyunsaturated fatty acids (LCPUFA) are used as a starting material, the method including a PL extracting step of extracting phospholipids (PL) from defatted cells obtained by extracting triglyceride (TG)-containing oil or fat from the lipid producing cells.
摘要翻译： 本发明提供了一种高效稳定地生产LCPUFA-PL的方法。 具体地说，本发明提供一种生产含有LCPUFA作为成分（LCPUFA-PL）的磷脂的方法，其中使用含有长链多不饱和脂肪酸（LCPUFA）的脂质产生细胞作为起始原料，该方法包括 PL提取步骤，从通过从脂质产生细胞中提取含甘油三酯（TG）的油或脂肪获得的脱脂细胞中提取磷脂（PL）。
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9.发明申请Producing Method of Phospholipids Including Long-Chain Polyunsaturated Fatty Acids as Constituents, and Use of Such Phospholipids 有权包括长链多不饱和脂肪酸作为成分的磷脂的生产方法，以及这种磷脂的使用公开(公告)号：US20100317622A1
公开(公告)日：2010-12-16
申请号：US12850389
申请日：2010-08-04
申请人： Hiroshi Kawashima , Motoo Sumida , Akiko Shiraishi
发明人： Hiroshi Kawashima , Motoo Sumida , Akiko Shiraishi
IPC分类号： A61K31/685 , C12P9/00 , C07F9/141 , C07F9/10 , A61K31/661 , A61K31/6615 , A61P25/00 , A61P3/06 , A23D9/02 , A23D7/00
CPC分类号： C12P9/00 , A23J7/00 , A23L33/115 , C07F9/103 , C12P7/6481 , C12P13/06
摘要： The present invention provides a method of efficiently and stably producing LCPUFA-PL. Specifically, the invention provides a method for producing phospholipids that contain LCPUFA as a constituent (LCPUFA-PL), wherein lipid producing cells producing lipids that contain long-chain polyunsaturated fatty acids (LCPUFA) are used as a starting material, the method including a PL extracting step of extracting phospholipids (PL) from defatted cells obtained by extracting triglyceride (TG)-containing oil or fat from the lipid producing cells.
摘要翻译： 本发明提供了一种有效且稳定地生产LCPUFA-PL的方法。 具体地说，本发明提供一种生产含有LCPUFA作为成分（LCPUFA-PL）的磷脂的方法，其中使用含有长链多不饱和脂肪酸（LCPUFA）的脂质产生细胞作为起始原料，该方法包括 PL提取步骤，从通过从脂质产生细胞中提取含甘油三酯（TG）的油或脂肪获得的脱脂细胞中提取磷脂（PL）。
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10.发明授权Phospholipids including long-chain polyunsaturated fatty acids as constituents, and use of such phospholipids 有权磷脂包括长链多不饱和脂肪酸作为成分，以及使用这种磷脂公开(公告)号：US07847113B2
公开(公告)日：2010-12-07
申请号：US10591329
申请日：2005-02-28
申请人： Hiroshi Kawashima , Motoo Sumida , Akiko Shiraishi
发明人： Hiroshi Kawashima , Motoo Sumida , Akiko Shiraishi
IPC分类号： C07F9/02
CPC分类号： C12P9/00 , A23J7/00 , A23L33/115 , C07F9/103 , C12P7/6481 , C12P13/06
摘要： The present invention provides a method of efficiently and stably producing LCPUFA-PL. Specifically, the invention provides a method for producing phospholipids that contain LCPUFA as a constituent (LCPUFA-PL), wherein lipid producing cells producing lipids that contain long-chain polyunsaturated fatty acids (LCPUFA) are used as a starting material, the method including a PL extracting step of extracting phospholipids (PL) from defatted cells obtained by extracting triglyceride (TG)-containing oil or fat from the lipid producing cells.
摘要翻译： 本发明提供了一种有效且稳定地生产LCPUFA-PL的方法。 具体地说，本发明提供一种生产含有LCPUFA作为成分（LCPUFA-PL）的磷脂的方法，其中使用含有长链多不饱和脂肪酸（LCPUFA）的脂质产生细胞作为起始原料，该方法包括 PL提取步骤，从通过从脂质产生细胞中提取含甘油三酯（TG）的油或脂肪获得的脱脂细胞中提取磷脂（PL）。
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