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1.发明授权公开(公告)号：US06861258B2
公开(公告)日：2005-03-01
申请号：US10085388
申请日：2002-02-28
CPC分类号： C12R1/20 , C12N15/74 , C12Q1/6897
摘要： A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.
摘要翻译： 一种用于在嗜热宿主中引入和稳定异源和重组基因的方法，其中定义可检测的宿主特征的特征基因从嗜热宿主中失活或缺失，导致表达不存在可检测宿主特征的经修饰的嗜热宿主。 将感兴趣的DNA片段与完整特征基因一起插入修饰的嗜热性宿主中，由此将可检测的宿主特征恢复到嗜热宿主，从而使得能够使用质粒载体检测和确认成功的转化并将DNA片段整合到 嗜热宿主的染色体。
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公开(公告)号：US06943006B2
公开(公告)日：2005-09-13
申请号：US10658691
申请日：2003-09-09
CPC分类号： C12P13/001 , C10G32/00 , C12N15/52 , C12P7/22
摘要： A method for selective cleavage of C—N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C—N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C—N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C—N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.
摘要翻译： 用于选择性切割CN键的方法将编码至少一种适用于将咔唑转化为2-氨基联苯-2,3-二醇的酶的基因与编码适合于选择性裂解2-氨基联苯中的CN键的酰胺酶的基因组合 -2,3-二醇，形成编码所述咔唑的两个CN键切割的操纵子。 将操纵子插入宿主培养物中，其继而与咔唑接触，导致咔唑的两个C-N键的选择性裂解。 还公开了组成型表达咔唑降解性状的新微生物和使用该微生物降解咔唑的方法。
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3.发明授权Enzyme from Rhodococcus rhodochrous ATCC 53968, Bacillus sphaericus ATCC 53969 or a mutant thereof for cleavage of organic C--S bonds 失效来自玫瑰色红球菌ATCC 53968，球形芽孢杆菌ATCC 53969或其有机C-S键切割的突变体的酶
公开(公告)号：US5516677A
公开(公告)日：1996-05-14
申请号：US270456
申请日：1994-07-05
申请人： John J. Kilbane, II
发明人： John J. Kilbane, II
IPC分类号： C10L1/14 , C07K1/113 , C10G32/00 , C10L9/00 , C12N1/20 , C12N9/00 , C12P1/04 , C12P3/00 , C12P21/06 , C12R1/07 , C12R1/365 , C12S1/02 , C12N9/14 , C12N1/12 , C12P11/00
CPC分类号： C12R1/00 , C07K1/1133 , C10G32/00 , C12N1/20 , C12P21/06 , C12P3/00 , C12R1/01 , C12R1/07 , Y10S435/822 , Y10S435/832
摘要： A process for organic C--S bond cleavage of a sulfur-containing organic carbonaceous material by contacting the carbonacous material with a sulfur specific reactant of membrane fragments, an enzyme, or a composition of enzymes having the ability to selectively react with sulfur by cleavage of organic C--S bonds. Preferred are sulfur specific reactants associated with cell membranes of Rhodococcus rhodochrous strain ATCC No. 53968 and Bacillus sphaericus strain ATCC No. 53969 and their derivatives which have the ability to selectively react with organic sulfur of sulfur-containing organic carbonaceous material by cleavage of organic C--S bonds.
摘要翻译： 通过使碳质材料与膜片段的酶的特异性反应物，酶或具有通过有机CS裂解选择性地与硫反应的能力的酶的组合物接触含硫有机碳质材料的有机CS键裂解的方法 债券。 优选的是与玫瑰色红球菌菌株ATCC No.53968和球形芽孢杆菌菌株ATCC No.53699的细胞膜相关的硫特异性反应物及其衍生物，其具有通过有机CS的裂解与含硫有机碳质材料的有机硫选择性反应的能力 债券。
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公开(公告)号：US06221651B1
公开(公告)日：2001-04-24
申请号：US09564642
申请日：2000-05-03
IPC分类号： C12N120
CPC分类号： C12R1/38 , C12N1/20 , Y10S435/874
摘要： A mutant Pseudomonas ayucida strain ATCC N° PTA-806 which is able to selectively cleave organic C—N bonds and reduce the nitrogen content of organic carbonaceous materials is described.
摘要翻译： 描述了能够有选择地劈开有机C-N键并降低有机碳质材料的氮含量的突变体的假单胞菌（Aseudomonas ayucida）菌株ATCC N PTA-806
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5.发明授权Microemulsion process for direct biocatalytic desulfurization of organosulfur molecules 失效用于直接生物催化脱硫有机硫分子的微乳液法
公开(公告)号：US5358870A
公开(公告)日：1994-10-25
申请号：US897314
申请日：1992-06-11
IPC分类号： C10L1/14 , C07K1/113 , C10G32/00 , C10L9/00 , C12N1/20 , C12N9/00 , C12P1/04 , C12P3/00 , C12P21/06 , C12R1/07 , C12R1/365 , C12S1/02 , C12N1/00 , C12N1/12 , C12N9/14
CPC分类号： C07K1/1133 , C10G32/00 , C12N1/20 , C12P21/06 , C12P3/00 , C12R1/00 , C12R1/01 , C12R1/07 , Y10S435/822 , Y10S435/832
摘要： A method of desulfurizing a petroleum liquid containing organosulfur molecules is disclosed. The method relies upon the use of an aqueous biocatalytic agent comprising, in preferred embodiments, a substantially cell free extract of a microorganism which functionally expresses an enzyme capable of selectively cleaving organic carbon-sulfur bonds even in sulfur-bearing heterocycles, wherein the extract contains a substantial proportion of the total activity of said enzyme expressed by the microorganism. An emulsion, preferably a microemulsion, is formed between this biocatalytic agent and the petroleum liquid. Reversible microemulsions are particularly preferred, due to facilitated recovery of a desulfurized petroleum liquid at the conclusion of treatment. The invention described is particularly well suited to the desulfurization of petroleum liquids having a high relative abundance of refractory organosulfur molecules, such as dibenzothiophene.
摘要翻译： 公开了一种含有有机硫分子的石油液体脱硫方法。 该方法依赖于使用水性生物催化剂，其在优选的实施方案中包括微生物的基本上无细胞的提取物，其功能性表达即使在含硫杂环中也能选择性地裂解有机碳 - 硫键的酶，其中所述提取物包含 由微生物表达的所述酶的总活性的相当大的比例。 在该生物催化剂和石油液体之间形成乳液，优选微乳液。 由于在处理结束时促进脱硫石油液体的回收，所以可逆微乳液是特别优选的。 所描述的发明特别适用于具有高相对含量的难熔有机硫分子如二苯并噻吩的石油液体的脱硫。
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6.发明授权Bacterial cleavage of only organic C-N bonds of carbonaceous materials to reduce nitrogen content 有权细菌裂解只有碳质材料的有机C-N键才能减少氮含量公开(公告)号：US06541240B1
公开(公告)日：2003-04-01
申请号：US09449613
申请日：1999-11-30
IPC分类号： B09B300
CPC分类号： C12R1/07 , C12N1/20 , C12P17/06 , Y10S435/822 , Y10S435/832 , Y10S435/874
摘要： A microbial process is provided for selective cleavage of only organic C—N bonds while leaving C—C bonds intact which may be used for reducing the nitrogen content of nitrogen-containing organic carbonaceous materials. Microorganisms of Pseudomonas ayucida have been found which have the ability of selective cleavage of organic C—N bonds. A particularly preferred microorganism is Pseudomonas ayucida strain ATCC No PTA-806. Other microorganisms useful in the cleavage of organic C—N bonds are Aneurinibacillus sp, Pseudomonas stutzeri, Yokenella sp. and Pseudomonas nitroreducens.
摘要翻译： 提供微生物方法用于选择性裂解仅有机C-N键，同时保留C-C键，这可用于降低含氮有机碳质材料的氮含量。 已经发现具有选择性裂解有机C-N键的能力的恶臭假单胞菌的微生物。 特别优选的微生物是假单胞菌（Aseudomonas ayucida）菌株ATCC No PTA-806。 用于切割有机C-N键的其他微生物是火鸡耳菌属，Streptomonas stutzeri，Yokenella sp。 和硝酸假单胞菌（Pseudomonas nitroreducens）。
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公开(公告)号：US06204048B1
公开(公告)日：2001-03-20
申请号：US09449615
申请日：1999-11-30
IPC分类号： C12N120
CPC分类号： C12R1/38 , C12N1/20 , Y10S435/874
摘要： A mutant Pseudomonas ayucida strain No PTA-806 which is able to selectively cleave organic C—N bonds and reduce the nitrogen content of organic carbonaceous materials is described.
摘要翻译： 描述了能够选择性地切割有机C-N键并降低有机碳质材料的氮含量的突变体假单胞菌（Aseudomonas ayucida）No PTA-806。
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公开(公告)号：US5750065A
公开(公告)日：1998-05-12
申请号：US8212
申请日：1993-01-25
申请人： John J. Kilbane, II
发明人： John J. Kilbane, II
摘要： A method for producing biosorbents for adsorption of PCB's in which at least one proteinaceous material is contacted with a molar excess of at least one PCB congener mixture, forming a protein/PCB congener mixture. The protein/PCB congener mixture is dried after which the PCB congeners are extracted from the dried protein/PCB congener mixture forming a biosorbent in the form of an imprinted protein.
摘要翻译： 一种用于生产用于吸附PCB的生物吸附剂的方法，其中至少一种蛋白质材料与摩尔过量的至少一种PCB同类物混合物接触，形成蛋白质/ PCB同类物混合物。 将蛋白质/ PCB同种物混合物干燥，之后将PCB同源物从形成印迹蛋白质形式的生物吸附剂的干燥的蛋白质/ PCB同种物混合物中提取。
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公开(公告)号：US5610065A
公开(公告)日：1997-03-11
申请号：US402421
申请日：1995-03-10
申请人： Robert L. Kelley , Andy H. Hill , Vipul J. Srivastava , W. Kennedy Gauger , John J. Kilbane, II
发明人： Robert L. Kelley , Andy H. Hill , Vipul J. Srivastava , W. Kennedy Gauger , John J. Kilbane, II
摘要： A process for improved remediation of polynuclear aromatic hydrocarbon and/or polychlorinated hydrocarbon contaminated solid materials by integrated chemical/biological treatment comprising contacting the polynuclear aromatic hydrocarbon and/or polychlorinated hydrocarbon for chemical treatment with hydrogen peroxide in the presence of ferrous ion in liquid solution at a temperature of about 10.degree. C. to about 100.degree. C., oxidizing the polynuclear aromatic hydrocarbon materials and/or polychlorinated hydrocarbon materials and producing more readily biodegradable hydrocarbon product materials, and then biodigesting the product materials by aerobic and/or anaerobic biodigestion. A lower alcohol added to the chemical treatment in accordance with one embodiment further enhances oxidation of 4- to 6-ring aromatic hydrocarbon contaminants.
摘要翻译： 一种用于通过综合化学/生物处理改进多核芳烃和/或多氯烃污染的固体物质的修复的方法，包括在亚铁离子存在下在液体溶液中使多核芳烃和/或多氯代烃与过氧化氢接触以进行化学处理 温度约10℃至约100℃，氧化多核芳烃材料和/或多氯烃材料并生产更容易生物降解的烃产品材料，然后通过有氧和/或厌氧生物消化生物消化产物材料。 根据一个实施方案添加到化学处理中的低级醇进一步增强4-至6-环芳族烃污染物的氧化。
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公开(公告)号：US5104801A
公开(公告)日：1992-04-14
申请号：US461389
申请日：1990-01-05
申请人： John J. Kilbane, II
发明人： John J. Kilbane, II
摘要： A mutant Rhodococcus rhodochrous strain ATCC No. 53968 which has the property of sulfur removal and sulfur metabolism by selective cleavage of C-S bonds in organic carbonaceous materials.
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