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14 条记录 (耗时 0.027 秒)

    Microemulsion process for direct biocatalytic desulfurization of organosulfur molecules
    1.
    发明授权
    Microemulsion process for direct biocatalytic desulfurization of organosulfur molecules 失效
    用于直接生物催化脱硫有机硫分子的微乳液法

    公开(公告)号：US5358870A

    公开(公告)日：1994-10-25

    申请号：US897314

    申请日：1992-06-11

    申请人： Daniel J. Monticello ,  John J. Kilbane, II

    发明人： Daniel J. Monticello ,  John J. Kilbane, II

    IPC分类号： C10L1/14 ,  C07K1/113 ,  C10G32/00 ,  C10L9/00 ,  C12N1/20 ,  C12N9/00 ,  C12P1/04 ,  C12P3/00 ,  C12P21/06 ,  C12R1/07 ,  C12R1/365 ,  C12S1/02 ,  C12N1/00 ,  C12N1/12 ,  C12N9/14

    CPC分类号： C07K1/1133 ,  C10G32/00 ,  C12N1/20 ,  C12P21/06 ,  C12P3/00 ,  C12R1/00 ,  C12R1/01 ,  C12R1/07 ,  Y10S435/822 ,  Y10S435/832

    摘要： A method of desulfurizing a petroleum liquid containing organosulfur molecules is disclosed. The method relies upon the use of an aqueous biocatalytic agent comprising, in preferred embodiments, a substantially cell free extract of a microorganism which functionally expresses an enzyme capable of selectively cleaving organic carbon-sulfur bonds even in sulfur-bearing heterocycles, wherein the extract contains a substantial proportion of the total activity of said enzyme expressed by the microorganism. An emulsion, preferably a microemulsion, is formed between this biocatalytic agent and the petroleum liquid. Reversible microemulsions are particularly preferred, due to facilitated recovery of a desulfurized petroleum liquid at the conclusion of treatment. The invention described is particularly well suited to the desulfurization of petroleum liquids having a high relative abundance of refractory organosulfur molecules, such as dibenzothiophene.

    摘要翻译： 公开了一种含有有机硫分子的石油液体脱硫方法。 该方法依赖于使用水性生物催化剂，其在优选的实施方案中包括微生物的基本上无细胞的提取物，其功能性表达即使在含硫杂环中也能选择性地裂解有机碳 - 硫键的酶，其中所述提取物包含 由微生物表达的所述酶的总活性的相当大的比例。 在该生物催化剂和石油液体之间形成乳液，优选微乳液。 由于在处理结束时促进脱硫石油液体的回收，所以可逆微乳液是特别优选的。 所描述的发明特别适用于具有高相对含量的难熔有机硫分子如二苯并噻吩的石油液体的脱硫。

    System and method for introduction and stabilization of genes in Thermus sp.
    3.
    发明授权
    System and method for introduction and stabilization of genes in Thermus sp. 失效
    引入和稳定栖热菌属基因的系统和方法

    公开(公告)号：US06861258B2

    公开(公告)日：2005-03-01

    申请号：US10085388

    申请日：2002-02-28

    申请人： Kevin J. Kayser ,  Ho-Shin Park ,  John J. Kilbane, II

    发明人： Kevin J. Kayser ,  Ho-Shin Park ,  John J. Kilbane, II

    IPC分类号： C12N1/21 ,  C12N15/74 ,  C12Q1/68 ,  C12P21/00

    CPC分类号： C12R1/20 ,  C12N15/74 ,  C12Q1/6897

    摘要： A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.

    摘要翻译： 一种用于在嗜热宿主中引入和稳定异源和重组基因的方法，其中定义可检测的宿主特征的特征基因从嗜热宿主中失活或缺失，导致表达不存在可检测宿主特征的经修饰的嗜热宿主。 将感兴趣的DNA片段与完整特征基因一起插入修饰的嗜热性宿主中，由此将可检测的宿主特征恢复到嗜热宿主，从而使得能够使用质粒载体检测和确认成功的转化并将DNA片段整合到 嗜热宿主的染色体。

    Bacterial cleavage of only organic C-N bonds of carbonaceous materials to reduce nitrogen content
    5.
    发明授权
    Bacterial cleavage of only organic C-N bonds of carbonaceous materials to reduce nitrogen content 有权
    细菌裂解只有碳质材料的有机C-N键才能减少氮含量

    公开(公告)号：US06541240B1

    公开(公告)日：2003-04-01

    申请号：US09449613

    申请日：1999-11-30

    申请人： John J. Kilbane, II ,  Claudia Maria Soares Ribeiro ,  Mônica Moreira Linhares

    发明人： John J. Kilbane, II ,  Claudia Maria Soares Ribeiro ,  Mônica Moreira Linhares

    IPC分类号： B09B300

    CPC分类号： C12R1/07 ,  C12N1/20 ,  C12P17/06 ,  Y10S435/822 ,  Y10S435/832 ,  Y10S435/874

    摘要： A microbial process is provided for selective cleavage of only organic C—N bonds while leaving C—C bonds intact which may be used for reducing the nitrogen content of nitrogen-containing organic carbonaceous materials. Microorganisms of Pseudomonas ayucida have been found which have the ability of selective cleavage of organic C—N bonds. A particularly preferred microorganism is Pseudomonas ayucida strain ATCC No PTA-806. Other microorganisms useful in the cleavage of organic C—N bonds are Aneurinibacillus sp, Pseudomonas stutzeri, Yokenella sp. and Pseudomonas nitroreducens.

    摘要翻译： 提供微生物方法用于选择性裂解仅有机C-N键，同时保留C-C键，这可用于降低含氮有机碳质材料的氮含量。 已经发现具有选择性裂解有机C-N键的能力的恶臭假单胞菌的微生​​物。 特别优选的微生物是假单胞菌（Aseudomonas ayucida）菌株ATCC No PTA-806。 用于切割有机C-N键的其他微生物是火鸡耳菌属，Streptomonas stutzeri，Yokenella sp。 和硝酸假单胞菌（Pseudomonas nitroreducens）。

    Adsorption of PCB's using biosorbents
    7.
    发明授权
    Adsorption of PCB's using biosorbents 失效
    使用生物吸附剂吸附PCB

    公开(公告)号：US5750065A

    公开(公告)日：1998-05-12

    申请号：US8212

    申请日：1993-01-25

    申请人： John J. Kilbane, II

    发明人： John J. Kilbane, II

    IPC分类号： B01J20/24 ,  B08B9/04 ,  B09C1/10 ,  C12N11/02 ,  B29B17/00

    CPC分类号： C12N11/02 ,  B01J20/24 ,  B08B9/055 ,  B09C1/10

    摘要： A method for producing biosorbents for adsorption of PCB's in which at least one proteinaceous material is contacted with a molar excess of at least one PCB congener mixture, forming a protein/PCB congener mixture. The protein/PCB congener mixture is dried after which the PCB congeners are extracted from the dried protein/PCB congener mixture forming a biosorbent in the form of an imprinted protein.

    摘要翻译： 一种用于生产用于吸附PCB的生物吸附剂的方法，其中至少一种蛋白质材料与摩尔过量的至少一种PCB同类物混合物接触，形成蛋白质/ PCB同类物混合物。 将蛋白质/ PCB同种物混合物干燥，之后将PCB同源物从形成印迹蛋白质形式的生物吸附剂的干燥的蛋白质/ PCB同种物混合物中提取。

    Integrated chemical/biological treatment of organic waste
    8.
    发明授权
    Integrated chemical/biological treatment of organic waste 失效
    有机废物综合化学/生物处理

    公开(公告)号：US5610065A

    公开(公告)日：1997-03-11

    申请号：US402421

    申请日：1995-03-10

    申请人： Robert L. Kelley ,  Andy H. Hill ,  Vipul J. Srivastava ,  W. Kennedy Gauger ,  John J. Kilbane, II

    发明人： Robert L. Kelley ,  Andy H. Hill ,  Vipul J. Srivastava ,  W. Kennedy Gauger ,  John J. Kilbane, II

    IPC分类号： A62D3/00 ,  A62D3/02 ,  B09C1/08 ,  B09C1/10 ,  C12P1/04 ,  C12S3/02

    CPC分类号： B09C1/10 ,  B09C1/08 ,  C12P1/04

    摘要： A process for improved remediation of polynuclear aromatic hydrocarbon and/or polychlorinated hydrocarbon contaminated solid materials by integrated chemical/biological treatment comprising contacting the polynuclear aromatic hydrocarbon and/or polychlorinated hydrocarbon for chemical treatment with hydrogen peroxide in the presence of ferrous ion in liquid solution at a temperature of about 10.degree. C. to about 100.degree. C., oxidizing the polynuclear aromatic hydrocarbon materials and/or polychlorinated hydrocarbon materials and producing more readily biodegradable hydrocarbon product materials, and then biodigesting the product materials by aerobic and/or anaerobic biodigestion. A lower alcohol added to the chemical treatment in accordance with one embodiment further enhances oxidation of 4- to 6-ring aromatic hydrocarbon contaminants.

    摘要翻译： 一种用于通过综合化学/生物处理改进多核芳烃和/或多氯烃污染的固体物质的修复的方法，包括在亚铁离子存在下在液体溶液中使多核芳烃和/或多氯代烃与过氧化氢接触以进行化学处理 温度约10℃至约100℃，氧化多核芳烃材料和/或多氯烃材料并生产更容易生物降解的烃产品材料，然后通过有氧和/或厌氧生物消化生物消化产物材料。 根据一个实施方案添加到化学处理中的低级醇进一步增强4-至6-环芳族烃污染物的氧化。