公开(公告)号：US20170253921A1

公开(公告)日：2017-09-07

申请号：US15518016

申请日：2015-10-13

Applicant: Life Technologies Corporation ,  Life Technologies Corporation

Inventor： Chunmei LIU ,  Shoulian DONG ,  Junko STEVENS

IPC: C12Q1/68

CPC classification number: C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2537/16 ,  C12Q2537/161 ,  C12Q2545/107 ,  C12Q2545/113 ,  C12Q2600/16

Abstract: The present specification relates to methods, compositions, and kits for determining the presence of the number of copies of a gene. The present disclosure provides methods and compositions to determine zygosity in transgenic plants, number of transgenes in a plant or animal, karyotyping, determine CNV in human and animal samples, diagnose and detect diseases and conditions that are characterized by having variations in the number of copies of one or more genes.

公开(公告)号：US20210277448A1

公开(公告)日：2021-09-09

申请号：US17301040

申请日：2021-03-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ferrier LE ,  Kalpith RAMAMOORTHI ,  Joyce WILDE ,  Nicole FANTIN ,  Jordan LANG ,  David DUPONT ,  Junko STEVENS

IPC: C12Q1/686 ,  C12Q1/6844

Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.

公开(公告)号：US20180282797A1

公开(公告)日：2018-10-04

申请号：US15927572

申请日：2018-03-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C12Q1/686 ,  C07K16/00 ,  C12N9/00

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20200370109A1

公开(公告)日：2020-11-26

申请号：US16947727

申请日：2020-08-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C12N9/00 ,  C07K16/00 ,  C12Q1/686

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20170292144A1

公开(公告)日：2017-10-12

申请号：US15481021

申请日：2017-04-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Ferrier LE ,  Kalpith RAMAMOORTHI ,  Joyce WILDE ,  Nicole FANTIN ,  Jordan LANG ,  David DUPONT ,  Junko STEVENS

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01D19/0404 ,  B01D19/0409 ,  B01D19/0413 ,  B01D19/0418 ,  B01D19/0422 ,  C12Q1/6846 ,  C12Q2525/125

Abstract: Compositions, methods, and kits for synthesizing, detecting, and/or quantifying nucleic acids are provided herein. Embodiments comprise a nucleic acid amplification composition comprising a thermostable DNA polymerase and agents which improve nucleic acid synthesis, amplification, detection, and/or quantification of nucleic acid targets in a crude extract or crude lysate sample.

公开(公告)号：US20240392394A1

公开(公告)日：2024-11-28

申请号：US18262672

申请日：2022-01-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Pius BRZOSKA ,  Joyce WILDE ,  Manohar FURTADO ,  Sara KENDRICK ,  Camilla ULEKLEIV ,  Junko STEVENS ,  Kamini VARMA ,  Kathleen HAYASHIBARA

IPC: C12Q1/70

Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of SARS-CoV-2, including SARS-CoV-2 variants, or other coronaviruses from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample.

公开(公告)号：US20240182958A1

公开(公告)日：2024-06-06

申请号：US18465581

申请日：2023-09-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

公开(公告)号：US20210324461A1

公开(公告)日：2021-10-21

申请号：US17250610

申请日：2019-08-16

Applicant: Life Technologies Corporation

Inventor： Joyce WILDE ,  Shoulian DONG ,  Carentine LAU ,  Junko STEVENS ,  Carmen GJERSTAD

IPC: C12Q1/6858 ,  C12Q1/6886

Abstract: This disclosure relates to reagents, mixtures, kits and methods for use in detecting low-frequency target polynucleotides, such as rare allelic variants.

公开(公告)号：US20140099644A1

公开(公告)日：2014-04-10

申请号：US13918768

申请日：2013-06-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Carole BORNARTH ,  Michael LAU ,  Junko STEVENS

IPC: C12Q1/68

CPC classification number: C12Q1/6848 ,  C07K16/00 ,  C12N9/00 ,  C12Q1/686 ,  C12Q2527/125 ,  C12Q2547/107 ,  C12Q2549/101

Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.

Abstract translation: 本公开涉及用于扩增靶核酸的组合物，方法和试剂盒，同时使用包含至少两种不同热启动机制的双重热启动反应混合物减少非特异性扩增和不期望的扩增产物。