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    Culturing mammalian cells in contact with cell surface proteins
    6.
    发明授权
    Culturing mammalian cells in contact with cell surface proteins 失效
    培养与细胞表面蛋白质接触的哺乳动物细胞

    公开(公告)号：US06251672B1

    公开(公告)日：2001-06-26

    申请号：US09197470

    申请日：1998-11-23

    申请人： Manfred Kubbies ,  Peter Dörmer ,  Petra Meissner

    发明人： Manfred Kubbies ,  Peter Dörmer ,  Petra Meissner

    IPC分类号： C12N500

    CPC分类号： C12N5/0068 ,  C12N2533/50

    摘要： Mammalian cells which require contact with cell surface proteins for activation, differentiation and/or proliferation are cultured in contact with an immobilized mammalian cell, with an immobilized membrane vesicle from a mammalian cell or with immobilized membrane vesicles from at least two mammalian cell populations. The mammalian cell or membrane vesicle can be modified with a first partner of a biological binding pair, and immobilized on a solid carrier via a second partner of the biological binding pair. Culturing can be with a first immobilized membrane vesicle and subsequently with a second immobilized membrane vesicle. The membrane vesicle contains part of the natural surface of a mammalian cell that includes a signal triggering surface protein. The membrane vesicle can be immobilized via a binding partner of a cell surface molecule.

    摘要翻译： 需要与细胞表面蛋白质接触以激活，分化和/或增殖的哺乳动物细胞与来自哺乳动物细胞的固定的膜囊泡或来自至少两个哺乳动物细胞群体的固定的膜囊泡接触固定的哺乳动物细胞培养。 哺乳动物细胞或膜囊泡可以用生物结合对的第一个配偶体修饰，并通过生物结合对的第二个配偶体固定在固体载体上。 可以用第一固定化膜囊泡培养，随后用第二固定化膜囊泡培养。 膜囊泡包含哺乳动物细胞的天然表面的一部分，其包括触发表面蛋白的信号。 膜泡可以通过细胞表面分子的结合配偶体固定。

    Method for Improved Selection of Rnai Transfectants
    9.
    发明申请
    Method for Improved Selection of Rnai Transfectants 审中-公开
    改进Rnai转染子选择的方法

    公开(公告)号：US20080248468A1

    公开(公告)日：2008-10-09

    申请号：US11792902

    申请日：2005-12-13

    申请人： Manfred Kubbies ,  Robert Macek ,  Carola Ries

    发明人： Manfred Kubbies ,  Robert Macek ,  Carola Ries

    IPC分类号： C12Q1/68 ,  C12N15/87 ,  C12N15/63 ,  C12N5/06 ,  C07K16/00

    CPC分类号： C12N15/111 ,  C12N2310/111 ,  C12N2310/14

    摘要： The present invention is directed to a method for inactivation of expression of a gene in a eucaryotic cell comprising (i) transfection of a eucaryotic cell with DNA comprising an expression cassette for expression of a cell surface protein and an expression cassette for expression of a RNAi compound, said compound being capable of inactivating expression of said gene, wherein said expression cassette for expression of a cell surface protein and said expression cassette for expression of a RNAi compound are located on the same vector DNA, and (ii) enrichment and/or selection of cells which express said cell surface protein.

    摘要翻译： 本发明涉及一种用于灭活真核细胞中基因表达的方法，包括（i）用包含用于表达细胞表面蛋白的表达盒和用于表达RNAi的表达盒的DNA转染真核细胞 化合物，所述化合物能够使所述基因的表达失活，其中用于表达细胞表面蛋白的表达盒和所述表达RNAi化合物的表达盒位于相同的载体DNA上，和（ii）富集和/或 选择表达所述细胞表面蛋白的细胞。