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公开(公告)号：US09783570B2
公开(公告)日：2017-10-10
申请号：US14127085
申请日：2012-06-28
CPC分类号： C07K1/165 , C07K1/18 , C07K1/20 , C07K1/36 , C07K14/5443 , C07K2319/30
摘要： Herein is reported a method for obtaining a polypeptide in monomeric form comprising the steps of a) providing a solution comprising the polypeptide m monomeric form and m aggregated form, wherein the ratio of monomeric to aggregated form is 4:1 or less as determined by size exclusion chromatography, b) performing a mixed-mode chromatography in bind-and-elute mode, or a hydrophobic interaction chromatography in flow-through mode, or a size-exclusion chromatography, and c) performing a weak cation exchange chromatography in bind-and-elute mode or flow-through mode, and thereby obtaining the polypeptide m monomeric form.
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2.发明申请公开(公告)号：US20140142283A1
公开(公告)日：2014-05-22
申请号：US14127085
申请日：2012-06-28
CPC分类号： C07K1/165 , C07K1/18 , C07K1/20 , C07K1/36 , C07K14/5443 , C07K2319/30
摘要： The present invention relates to methods for obtaining a polypeptide in a monomeric form, the method comprising a) providing a solution containing the polypeptide in monomeric form and in aggregated form, wherein the ratio of monomeric to aggregated form is 4:1 or less, as determined by size exclusion chromatography, b) performing mixed-mode chromatography in bind-and-elute mode, or hydrophobic interaction chromatography in flow-through mode, or a size-exclusion chromatography, and c) performing a weak cation exchange chromatography in bind-and-elute mode or flow-through mode, and thereby obtaining the polypeptide in monomeric form.
摘要翻译： 本发明涉及以单体形式获得多肽的方法，所述方法包括：a）提供含有单体形式和聚集形式的多肽的溶液，其中单体与聚集形式的比例为4:1或更低，如 通过尺寸排阻色谱法确定，b）以结合 - 洗脱模式进行混合模式色谱法，或以流通模式或尺寸排阻色谱法进行疏水相互作用层析，以及c）在结合 - 洗脱模式或流通模式，从而获得单体形式的多肽。
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3.发明申请METHOD FOR PRODUCING A LIPID PARTICLE, THE LIPID PARTICLE ITSELF AND ITS USE 审中-公开生产脂肪颗粒的方法，脂质颗粒及其用途公开(公告)号：US20120190609A1
公开(公告)日：2012-07-26
申请号：US13217536
申请日：2011-08-25
申请人： Martin Bader , Monika Baehner , Adelbert Grossmann , Anton Jochner , Hubert Kettenberger , Silke Mohl
发明人： Martin Bader , Monika Baehner , Adelbert Grossmann , Anton Jochner , Hubert Kettenberger , Silke Mohl
CPC分类号： A61K9/1275 , A61K9/1277 , A61K38/1709 , C07K5/1008 , C07K14/47 , C07K14/775 , C07K2319/00
摘要： A method for producing a lipid particle comprising the following: i) providing a first solution comprising denatured apolipoprotein, ii) adding the first solution to a second solution comprising at least two lipids and a detergent but no apolipoprotein, and iii) removing the detergent from the solution obtained in ii) and thereby producing a lipid particle.
摘要翻译： 一种制备脂质颗粒的方法，包括以下步骤：i）提供包含变性载脂蛋白的第一溶液，ii）将第一溶液加入到包含至少两种脂质和洗涤剂但不含载脂蛋白的第二溶液中，和iii）将去污剂从 在ii）中获得的溶液，从而产生脂质颗粒。
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公开(公告)号：US20110086026A1
公开(公告)日：2011-04-14
申请号：US12843225
申请日：2010-07-26
申请人： Josef Endl , Maria Elena Fuentes , Yvo Graus , Adelbert Grossmann , Sebastian Neumann , Paul Parren , Frank Rebers , Joerg Thomas Regula , Ralf Schumacher , Stefan Seeber , Jan Olaf Stracke , Kay-Gunnar Stubenrauch , Jan Van De Winkel , Martine Van Vugt , Sandra Vereecken-Verploegen
发明人： Josef Endl , Maria Elena Fuentes , Yvo Graus , Adelbert Grossmann , Sebastian Neumann , Paul Parren , Frank Rebers , Joerg Thomas Regula , Ralf Schumacher , Stefan Seeber , Jan Olaf Stracke , Kay-Gunnar Stubenrauch , Jan Van De Winkel , Martine Van Vugt , Sandra Vereecken-Verploegen
IPC分类号： A61K39/395 , C07H21/00 , C07K16/00 , A61P11/06 , A61P37/02 , C12N5/10 , C12P21/02 , C12N15/63 , C12N1/00
CPC分类号： C07K16/2866 , C07K2317/21 , C07K2317/56 , C07K2317/76
摘要： An antibody binding to IL-13Rα1, inhibiting IL-13 bioactivity and comprising a variable heavy and a variable light chain, characterized in that the variable heavy chain amino acid sequence CDR3 of said antibody is selected from the group consisting of the heavy chain CDR3 sequences of SEQ ID NO: 1, 3, 5, 7 or 9 is useful in the treatment of asthma and allergic diseases.
摘要翻译： 与IL-13Rα1结合的抗体，抑制IL-13生物活性并包含可变重链和可变轻链的抗体，其特征在于所述抗体的可变重链氨基酸序列CDR3选自重链CDR3序列 的SEQ ID NO：1,3,5,7或9可用于治疗哮喘和过敏性疾病。
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公开(公告)号：US20100203589A1
公开(公告)日：2010-08-12
申请号：US12765229
申请日：2010-04-22
IPC分类号： C12P21/02
CPC分类号： C07K14/48
摘要： The invention relates to a method for producing biologically active β-NGF from the proform proNGF. After expressing the proform of the β-NGF in a prokaryotic host cell, the recombinant protein is isolated in the form of insoluble inactive aggregates (inclusion bodies). After the solubilization thereof in a strong denaturing agent and the subsequent conversion thereof into the natural conformation, which is determined by the disulfide bridges present in the natural β-NGF, biologically active β-NGF is obtained by subsequently splitting-off the prosequence.
摘要翻译： 本发明涉及从前体proNGF生产生物活性的NGF的方法。 在原核宿主细胞中表达生物素-NGF的形式后，重组蛋白以不溶性非活性聚集体（包涵体）的形式分离。 在将其溶解在强变性剂中并随后转化成通过存在于天然和/或生长因子中的二硫键确定的天然构象后，通过随后分离前体 。
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公开(公告)号：US07541163B2
公开(公告)日：2009-06-02
申请号：US11838730
申请日：2007-08-14
申请人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse
发明人： Phillip A. Patten , Sridhar Viswanathan , Torben Lauesgaard Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US20070025966A1
公开(公告)日：2007-02-01
申请号：US11531557
申请日：2006-09-13
申请人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
发明人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
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公开(公告)号：US06512123B2
公开(公告)日：2003-01-28
申请号：US10127100
申请日：2002-04-22
IPC分类号： C07D20982
CPC分类号： C07C259/06 , C07D209/88 , C07D313/12
摘要： Compounds of formula I wherein R1, R2, A, X, Y and Z have the meanings provided in the specification, and their enantiomers, diastereoisomers, racemates, pharmaceutically acceptable salts and mixtures thereof. These compounds have HDAC inhibitory activity for inhibiting cell proliferation. Also provided is a process of manufacturing these compounds.
摘要翻译： R1，R2，A，X，Y和Z中的式I化合物具有说明书中提供的含义及其对映异构体，非对映异构体，外消旋体，药学上可接受的盐及其混合物。 这些化合物具有抑制细胞增殖的HDAC抑制活性。 还提供了制造这些化合物的方法。
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公开(公告)号：US20050266465A1
公开(公告)日：2005-12-01
申请号：US11132722
申请日：2005-05-18
申请人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski , Stephens Adam
发明人： Phillip Patten , Sridhar Viswanathan , Torben Nissen , Anne Vogt , Harald Kropshofer , Ralf Schumacher , Stephan Fischer , Stefan Seeber , Adelbert Grossmann , Friederike Hesse , Andreas Schaubmar , Roberto Falkenstein , Hans Koll , Markus Dembowski , Stephens Adam
CPC分类号： A61K38/212 , A61K47/60 , C07K14/56 , Y02A50/463
摘要： The present invention provides interferon-alpha polypeptides and conjugates, and nucleic acids encoding the polypeptides. The invention also includes compositions comprising these polypeptides, conjugates, and nucleic acids; cells containing or expressing the polypeptides, conjugates, and nucleic acids; methods of making the polypeptides, conjugates, and nucleic acids; and methods of using the polypeptides, conjugates, and nucleic acids.
摘要翻译： 本发明提供了干扰素-α多肽和缀合物，以及编码多肽的核酸。 本发明还包括包含这些多肽，缀合物和核酸的组合物; 含有或表达多肽，缀合物和核酸的细胞; 制备多肽，缀合物和核酸的方法; 以及使用多肽，缀合物和核酸的方法。
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公开(公告)号：US5672691A
公开(公告)日：1997-09-30
申请号：US434718
申请日：1995-05-04
IPC分类号： C07K14/195 , C07K14/36 , C07K14/41 , C12N1/21 , C12N15/09 , C12N15/31 , C12P21/02 , C12R1/19 , G01N33/50 , G01N33/53 , C07K1/22
CPC分类号： C07K14/36
摘要： The present invention concerns a process for the isolation of recombinant core streptavidin in which host cells are transformed with a DNA coding for core streptavidin, the transformed host cells are cultured under suitable conditions, the DNA coding for core streptavidin is expressed and the recombinant core streptavidin is isolated from the host cells or the culture medium, wherein a DNA coding for core streptavidin is used which has (a) the nucleotide sequence shown in SEQ ID NO. 1 or (b) a nucleotide sequence corresponding to the nucleotide sequence (a) within the scope of the degeneracy of the genetic code.
摘要翻译： 本发明涉及分离重组核心链霉抗生物素蛋白的方法，其中宿主细胞用编码核心链霉抗生物素蛋白的DNA转化，转化的宿主细胞在合适的条件下培养，编码核心链霉抗生物素蛋白的DNA被表达，重组核心链霉亲和素 从宿主细胞或培养基中分离，其中使用编码核心链亲和素的DNA，其具有（a）SEQ ID NO： 1或（b）对应于遗传密码简并范围内的核苷酸序列（a）的核苷酸序列。
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