公开(公告)号：US20050191665A1

公开(公告)日：2005-09-01

申请号：US11021682

申请日：2004-12-23

申请人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

发明人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

IPC分类号： G01N21/65 ,  G01N33/543 ,  C12Q1/68 ,  G01J3/44 ,  G01N33/553

CPC分类号： G01N33/54346 ,  G01N21/658

摘要： Composite organic-inorganic nanoclusters (COINs) are provided that produce surface-enhanced Raman signals (SERS) when excited by a laser. The nanoclusters include metal particles and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanocluster and a wide variety of Raman-active organic compounds and combinations thereof can be incorporated into the nanocluster. In addition, polymeric microspheres containing the nanoclusters and methods of making them are also provided. The nanoclusters and microspheres can be used, for example, in assays for multiplex detection of biological molecules.

摘要翻译： 提供了复合有机 - 无机纳米团簇（COINs），当激光激发时产生表面增强拉曼信号（SERS）。 纳米团簇包括金属颗粒和拉曼活性有机化合物。 实现合适的SERS信号所需的金属在纳米簇中是固有的，并且可以将多种拉曼活性有机化合物及其组合掺入纳米团簇中。 此外，还提供了含有纳米簇的聚合物微球及其制备方法。 纳米团簇和微球可用于例如用于多重检测生物分子的测定中。

公开(公告)号：US20050147963A1

公开(公告)日：2005-07-07

申请号：US10748336

申请日：2003-12-29

申请人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

发明人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

IPC分类号： G01N21/65 ,  G01N33/543 ,  C12Q1/70 ,  C12M1/34 ,  C12Q1/68

CPC分类号： G01N33/54346 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/658 ,  G01N2021/6482 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

摘要： Composite organic-inorganic nanoparticles (COIN) are provided that produce surface-enhanced Raman signals when excited by a laser. The nanoparticles include metallic colloids and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanoparticle, and a wide variety of Raman-active organic compounds can be incorporated into the particle. Indeed, a large number of unique Raman signatures can be created by employing nanoparticles containing Raman-active organic compounds of different structures, mixtures, and ratios. Thus, nanoparticles and methods described herein are useful for the simultaneous detection of many analytes in a mixture, resulting in rapid qualitative analysis of a mixture. In addition, since many Raman-active organic compounds can be incorporated into a single nanoparticle, the SERS signal from a single COIN particle is strong relative to SERS signals obtained from Raman-active materials that do not contain the nanoparticles described herein.

摘要翻译： 提供了复合有机 - 无机纳米粒子（COIN），当激光激发时产生表面增强的拉曼信号。 纳米颗粒包括金属胶体和拉曼活性有机化合物。 实现合适的SERS信号所需的金属在纳米颗粒中是固有的，并且可以将多种拉曼活性有机化合物掺入颗粒中。 实际上，通过使用含有不同结构，混合物和比例的拉曼活性有机化合物的纳米颗粒，可以产生大量独特的拉曼特征。 因此，本文所述的纳米颗粒和方法可用于同时检测混合物中的许多分析物，导致混合物的快速定性分析。 另外，由于许多拉曼活性有机化合物可以并入单个纳米颗粒中，所以来自单个COIN颗粒的SERS信号相对于不含本文所述纳米颗粒的拉曼活性材料获得的SERS信号是强的。

公开(公告)号：US20050142567A1

公开(公告)日：2005-06-30

申请号：US10830422

申请日：2004-04-21

申请人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

发明人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew Berlin

IPC分类号： G01N21/65 ,  G01N33/543 ,  C12Q1/68 ,  G01N33/553

CPC分类号： G01N33/54346 ,  G01N21/6428 ,  G01N21/6458 ,  G01N21/658 ,  G01N2021/6482 ,  G01N2021/653 ,  G01N2021/655 ,  G01N2021/656

摘要： Composite organic-inorganic nanoparticles (COIN) and clusters of such nanoparticles are provided that produce surface-enhanced Raman signals when excited by a laser. The nanoparticles include metallic colloids and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanoparticle, and a wide variety of Raman-active organic compounds can be incorporated into the particle. Methods for producing the nanoparticles and clusters of nanoparticles are also provided. In addition, polymeric microspheres containing the nanoparticles and clusters of nanoparticles and methods of making them are also provided. Methods for using the nanoparticles, clusters, and microspheres in assays for multiplex detection of biological molecules do not require signal amplification techniques.

摘要翻译： 提供复合有机 - 无机纳米颗粒（COIN）和这种纳米颗粒的簇，当激光激发时产生表面增强的拉曼信号。 纳米颗粒包括金属胶体和拉曼活性有机化合物。 实现合适的SERS信号所需的金属在纳米颗粒中是固有的，并且可以将多种拉曼活性有机化合物掺入颗粒中。 还提供了制备纳米颗粒和纳米颗粒簇的方法。 此外，还提供了包含纳米颗粒和纳米颗粒簇的聚合物微球及其制备方法。 在用于多重检测生物分子的测定中使用纳米颗粒，簇和微球的方法不需要信号放大技术。

公开(公告)号：US08062841B2

公开(公告)日：2011-11-22

申请号：US12147440

申请日：2008-06-26

申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C07H21/02 ,  C07H21/04 ,  C07K14/00

CPC分类号： G01N33/53 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/5438

摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a single spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

摘要翻译： 公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。 公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。 多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。 还披露了机器学习和数据分析方案。 将多个亲和力结合配偶体（每个由唯一报道分子标记）与样品接触，并采用单个谱来检测多个报道信号。 通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

公开(公告)号：US07351591B2

公开(公告)日：2008-04-01

申请号：US10814695

申请日：2004-03-30

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

IPC分类号： G01N33/553

CPC分类号： G01N21/658

摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

公开(公告)号：US20100240870A1

公开(公告)日：2010-09-23

申请号：US12658414

申请日：2010-02-10

申请人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew A. Berlin

发明人： Xing Su ,  Jingwu Zhang ,  Lei Sun ,  Andrew A. Berlin

IPC分类号： C07K14/765 ,  C07F1/10 ,  C07F15/00 ,  C07F5/06 ,  C07F1/12 ,  C07F1/08 ,  C07K16/00 ,  C07K14/00 ,  C07H21/00 ,  C07H23/00

CPC分类号： G01N33/54346 ,  G01N21/658

摘要： Composite organic-inorganic nanoclusters (COINs) are provided that produce surface-enhanced Raman signals (SERS) when excited by a laser. The nanoclusters include metal particles and a Raman-active organic compound. The metal required for achieving a suitable SERS signal is inherent in the nanocluster and a wide variety of Raman-active organic compounds and combinations thereof can be incorporated into the nanocluster. In addition, polymeric microspheres containing the nanoclusters and methods of making them are also provided. The nanoclusters and microspheres can be used, for example, in assays for multiplex detection of biological molecules.

摘要翻译： 提供了复合有机 - 无机纳米团簇（COINs），当激光激发时产生表面增强拉曼信号（SERS）。 纳米团簇包括金属颗粒和拉曼活性有机化合物。 实现合适的SERS信号所需的金属在纳米簇中是固有的，并且可以将多种拉曼活性有机化合物及其组合掺入纳米团簇中。 此外，还提供了含有纳米簇的聚合物微球及其制备方法。 纳米团簇和微球可用于例如用于多重检测生物分子的测定中。

公开(公告)号：US20080262989A1

公开(公告)日：2008-10-23

申请号：US12147243

申请日：2008-06-26

申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

IPC分类号： G06F17/50

CPC分类号： G01N33/53 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/5438

摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a single spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

摘要翻译： 公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。 公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。 多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。 还披露了机器学习和数据分析方案。 将多个亲和力结合配偶体（每个由唯一报道分子标记）与样品接触，并采用单个谱来检测多个报道信号。 通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

公开(公告)号：US07410763B2

公开(公告)日：2008-08-12

申请号：US11216112

申请日：2005-09-01

申请人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

发明人： Xing Su ,  Lei Sun ,  Mineo Yamakawa ,  Jingwu Zhang ,  Qing Ma ,  Tae-Woong Koo ,  Richard Jones

IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12M1/00

CPC分类号： G01N33/53 ,  B82Y15/00 ,  B82Y30/00 ,  G01N33/54366 ,  G01N33/5438

摘要： Method and device to collect multiplex data simultaneously in analyte detection and analyze the data by experimentally trained software (machine-learning) is disclosed. Various ways (magnetic particles and microcoils) are disclosed to collect multiple reporter (tag) signals. Multiplex detection can increase the biomolecule analysis efficiency by using small sample size and saving assay reagents and time. Machine learning and data analysis schemes are also disclosed. Multiple affinity binding partners, each labeled by a unique reporter, are contacted with a sample and a green spectrum is taken to detect multiple reporter signals. The spectrum is deconvoluted by experimentally trained software to identify multiple analytes.

摘要翻译： 公开了通过实验训练的软件（机器学习）在分析物检测中同时收集多重数据并分析数据的方法和装置。 公开了各种方式（磁性颗粒和微型线圈）以收集多个报道（标签）信号。 多重检测可以通过使用小样本量和节省测定试剂和时间来增加生物分子分析效率。 还披露了机器学习和数据分析方案。 将多个亲和力结合配偶体（各自由唯一报道分子标记）与样品接触，并采用绿色光谱来检测多个报道信号。 通过实验训练的软件对光谱进行去卷积，以识别多个分析物。

公开(公告)号：US07432113B2

公开(公告)日：2008-10-07

申请号：US11134749

申请日：2005-05-19

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Jingwu Zhang ,  Lei Sun

IPC分类号： G01N33/553

CPC分类号： G01N21/658

摘要： The present invention is based on the discovery that the methods described herein for the production of metallic colloids result in colloids exhibiting increased signal enhancement and reproducibility for the SERS detection of biomolecules. Thus, using the methods of the invention, a wide variety of biomolecules can be detected with a greater sensitivity and reliability.

公开(公告)号：US20080241569A1

公开(公告)日：2008-10-02

申请号：US11694293

申请日：2007-03-30

申请人： Haoyu Qin ,  Jingwu Zhang ,  Claire E. Dentinger ,  Lei Sun ,  Xing Su

发明人： Haoyu Qin ,  Jingwu Zhang ,  Claire E. Dentinger ,  Lei Sun ,  Xing Su

IPC分类号： B32B5/16

CPC分类号： G01N21/658 ,  B82Y15/00 ,  B82Y30/00 ,  Y10T428/12028 ,  Y10T428/2991

摘要： Embodiments of the present invention provide methods for coating nanoparticles with polymeric coatings and nanoparticles that are coated with polymeric coatings. The polymeric coatings typically comprise two or more layers wherein the first layer has a charge that is opposite to that of the second layer. In further embodiments, the nanoparticles that can act as labels or reporters are coated with polymeric coatings. Optionally, these reporter or label nanoparticles may be Raman-active, such that they provide a distinctive Raman signature upon excitation with electromagnetic radiation.

摘要翻译： 本发明的实施方案提供了用聚合物涂层和纳米颗粒涂覆聚合物涂层的方法。 聚合物涂层通常包含两层或更多层，其中第一层具有与第二层相反的电荷。 在另外的实施方案中，可以用作标记或报告物的纳米颗粒涂覆有聚合物涂层。 任选地，这些报告物或标记纳米颗粒可以是拉曼活性的，使得它们在用电磁辐射激发时提供独特的拉曼特征。