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    Detection of chromosomal disorders
    1.
    发明授权
    Detection of chromosomal disorders 失效
    检测染色体异常

    公开(公告)号：US07468249B2

    公开(公告)日：2008-12-23

    申请号：US10840208

    申请日：2004-05-05

    申请人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

    发明人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6837 ,  C12Q2537/143 ,  C12Q2531/113

    摘要： Methods for detecting in a single assay any one of multiple chromosomal disorders that result from aneuploidy or certain mutations, particularly microdeletions, and kits for use therein. A polymerase chain reaction (PCR) is carried out to amplify eukaryotic genomic DNA using a plurality of primer oligonucleotide pairs wherein one primer of each pair has a detectable label attached 5′ thereto. A plurality of the primer pairs are targeted to DNA segments of different chromosomes of interest which are indicative of potential chromosomal disorders, and one pair is targeted for a control gene. The amplified PCR products are purified, and single-stranded DNA having the detectable labels is obtained therefrom and hybridized with spots on a microarray that each contain DNA oligonucleotide probes having nucleotide sequences complementary to a nucleotide sequence of one strand of each segment. The microarray is imaged for presence of labels on its respective spots, and the absence or presence of chromosomal disorders as indicated by one or more of the targeted DNA segments of interest is diagnosed by first comparing the imaging results to the imaging of spots specific to the control gene and then to results obtained from imaging normal DNA.

    摘要翻译： 在单次测定中检测由非整倍体或某些突变，特别是微缺失引起的多种染色体紊乱中的任何一种以及用于其中的试剂盒的方法。 进行聚合酶链反应（PCR）以使用多个引物寡核苷酸对扩增真核基因组DNA，其中每对的一个引物具有附着5'的可检测标记。 多个引物对靶向不同染色体的DNA片段，其指示潜在的染色体病症，一对靶向对照基因。 纯化扩增的PCR产物，从其中获得具有可检测标记的单链DNA，并与微阵列上的斑点杂交，每个点均含有具有与每个片段的一条链的核苷酸序列互补的核苷酸序列的DNA寡核苷酸探针。 成像微阵列以在其各自的斑点上存在标记，并且通过首先将成像结果与成像特异性的斑点的成像进行比较来诊断由一个或多个目标DNA片段指示的染色体病症的不存在或存在 控制基因，然后从成像正常DNA获得的结果。

    Detection of chromosomal disorders
    2.
    发明申请
    Detection of chromosomal disorders 失效
    检测染色体异常

    公开(公告)号：US20050250111A1

    公开(公告)日：2005-11-10

    申请号：US10840208

    申请日：2004-05-05

    申请人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

    发明人： Zhiyi Xie ,  Soonkap Hahn ,  Tim Watanaskul

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6837 ,  C12Q2537/143 ,  C12Q2531/113

    摘要： Methods for detecting in a single assay any one of multiple chromosomal disorders that result from aneuploidy or certain mutations, particularly microdeletions, and kits for use therein. A polymerase chain reaction (PCR) is carried out to amplify eukaryotic genomic DNA using a plurality of primer oligonucleotide pairs wherein one primer of each pair has a detectable label attached 5′ thereto. A plurality of the primer pairs are targeted to DNA segments of different chromosomes of interest which are indicative of potential chromosomal disorders, and one pair is targeted for a control gene. The amplified PCR products are purified, and single-stranded DNA having the detectable labels is obtained therefrom and hybridized with spots on a microarray that each contain DNA oligonucleotide probes having nucleotide sequences complementary to a nucleotide sequence of one strand of each segment. The microarray is imaged for presence of labels on its respective spots, and the absence or presence of chromosomal disorders as indicated by one or more of the targeted DNA segments of interest is diagnosed by first comparing the imaging results to the imaging of spots specific to the control gene and then to results obtained from imaging normal DNA.

    摘要翻译： 在单次测定中检测由非整倍体或某些突变，特别是微缺失引起的多种染色体紊乱中的任何一种以及用于其中的试剂盒的方法。 进行聚合酶链反应（PCR）以使用多个引物寡核苷酸对扩增真核基因组DNA，其中每对的一个引物具有附着5'的可检测标记。 多个引物对靶向不同染色体的DNA片段，其指示潜在的染色体病症，一对靶向对照基因。 纯化扩增的PCR产物，从其中获得具有可检测标记的单链DNA，并与微阵列上的斑点杂交，每个点均含有具有与每个片段的一条链的核苷酸序列互补的核苷酸序列的DNA寡核苷酸探针。 成像微阵列以在其各自的斑点上存在标记，并且通过首先将成像结果与成像特异性的斑点的成像进行比较来诊断由一个或多个目标DNA片段指示的染色体病症的不存在或存在 控制基因，然后从成像正常DNA获得的结果。

    Detection of STRP, such as fragile X syndrome
    5.
    发明申请
    Detection of STRP, such as fragile X syndrome 审中-公开
    检测STRP，如脆性X综合征

    公开(公告)号：US20050191636A1

    公开(公告)日：2005-09-01

    申请号：US10791209

    申请日：2004-03-01

    申请人： Soonkap Hahn

    发明人： Soonkap Hahn

    IPC分类号： C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

    CPC分类号： C12Q1/6883 ,  C12Q2600/156

    摘要： Methods for detecting a short tandem repeat polymorphism (STRP), such as fragile X syndrome, wherein PCR is used to amplify nucleic acid along the chromosome in the genomic DNA which includes all of the STRs of interest plus a substantial contiguous segment of the nucleic acid adjacent to the STRs. Single-stranded product is then obtained, and colorimetric-labeled oligonucleotides which target for (i) STRs and (ii) the contiguous DNA segment are hybridized with this single-stranded product which is then bound to a solid phase and separated from the remainder of the target material. The labeled oligonucleotide target material is recovered by treatment with base and then hybridized to a microarray having a plurality of spots containing suitable oligonucleotide probes complementary thereto. Following hybridization, colorimetric intensities of the hybridized labeled target material present at specific spots on the microarray are measured to obtain individual values which are compared with results from known control samples to accurately quantify the number of STRs in the region of interest of the DNA being analyzed.

    摘要翻译： 用于检测短串联重复多态性（STRP）的方法，例如脆性X综合征，其中使用PCR来扩增基因组DNA中沿着染色体的核酸，其包括所有感兴趣的STR加上核酸的相当连续的片段 毗邻STR。 然后获得单链产物，将靶向（i）STR和（ii）连续DNA片段的比色标记的寡核苷酸与该单链产物杂交，然后将其与固相结合并与其余部分分离 目标材料。 通过用碱处理回收标记的寡核苷酸靶物质，然后与具有多个含有与其互补的寡核苷酸探针的斑点的微阵列杂交。 杂交后，测量存在于微阵列特定斑点上的杂交标记的靶物质的比色强度，以获得与已知对照样品的结果进行比较以准确定量所分析的DNA感兴趣区域中STR的数目的个体值 。

    Child-birth assisting system
    6.
    发明授权
    Child-birth assisting system 失效
    育儿辅助系统

    公开(公告)号：US5645563A

    公开(公告)日：1997-07-08

    申请号：US377145

    申请日：1995-01-24

    申请人： Soonkap Hahn ,  John Merritt

    发明人： Soonkap Hahn ,  John Merritt

    IPC分类号： A61B5/00 ,  A61B17/135 ,  A61B17/42

    CPC分类号： A61B17/42 ,  A61B17/1355

    摘要： The childbirth-assisting device uses an automatically synchronized expandable pneumatic girdle to externally augment the secondary force of labor. The girdle through which the pressure is applied is fitted around the abdomen of the woman. An inflatable bladder within the girdle is inflated to create a downward pressure on the abdomen upon detection of a contraction. External pressure monitors which detect contractions may be attached directly to the girdle, and the girdle is configured to assure that the bladder is correctly positioned at all times. A member may be provided to prevent multiple uses of a girdle to assure the integrity of the girdle during use.

    摘要翻译： 分娩辅助装置使用自动同步的可膨胀气动腰带来外部增加第二劳动力。 施加压力的腰带围绕着女人的腹部。 腰围内的充气气囊膨胀，以便在检测到收缩时在腹部产生向下的压力。 检测收缩的外部压力监视器可以直接附接到腰带，并且腰带被配置为确保膀胱在任何时候都被正确定位。 可以提供一个构件以防止腰带的多次使用以确保在使用过程中腰带的完整性。

    CO.sub.2 sensor using a hydrophilic polyurethane matrix and process for manufacturing
    7.
    发明授权
    CO.sub.2 sensor using a hydrophilic polyurethane matrix and process for manufacturing 失效
    使用亲水性聚氨酯基质的二氧化碳传感器和制造方法

    公开(公告)号：US5326531A

    公开(公告)日：1994-07-05

    申请号：US989102

    申请日：1992-12-11

    申请人： Soonkap Hahn ,  Alan Nelson ,  Monte Bennett ,  Henry K. Hui

    发明人： Soonkap Hahn ,  Alan Nelson ,  Monte Bennett ,  Henry K. Hui

    IPC分类号： A61B5/00 ,  G01N21/64 ,  G01N21/77 ,  G01N21/00 ,  G01N33/50

    CPC分类号： G01N21/643 ,  A61B5/1455 ,  G01N21/7703 ,  G01N2021/772 ,  G01N2021/7786

    摘要： The carbon dioxide sensor includes a CO.sub.2 sensing matrix formed from a hydrophilic, gas permeable, cross-linked polyurethane. The polyurethane matrix is formed from a polyurethane pre-polymer mixture which cross links when mixed with an aqueous carbon dioxide buffer solution. The dye-buffer solution is physically entrapped as a homogeneous dispersion in the polymer matrix. The matrix is applied to the exposed glass core of an optical fiber, which may first be coated with a primer to allow the polyurethane matrix to bond firmly to the tip of the optical fiber. The hydrophilic polymeric matrix is preferably coated with a hydrophobic material to help avoid leaching of the dye from the matrix, and to prevent diffusion of hydronium ions into the sensing matrix. The hydrophobic polymer overcoat may thereafter be cured by heat treatment. The hydrophobic coating may also contain a light reflective material such as TiO.sub.2.

    摘要翻译： 二氧化碳传感器包括由亲水的，透气的交联聚氨酯形成的CO 2感测基质。 聚氨酯基质由与二氧化碳水溶液溶液混合时交联的聚氨酯预聚物混合物形成。 染料缓冲溶液在聚合物基质中物理截留为均匀分散体。 将基质施加到光纤的暴露的玻璃芯上，其可以首先用底漆涂布，以使聚氨酯基质牢固地结合到光纤的末端。 亲水性聚合物基质优选用疏水材料涂覆以帮助避免染料从基质中浸出，并防止水合氢离子扩散到感测基质中。 此后，疏水聚合物外涂层可以通过热处理固化。 疏水涂层还可以含有诸如TiO 2的光反射材料。

    Child birth assisting system
    8.
    发明授权
    Child birth assisting system 失效
    儿童出生辅助系统

    公开(公告)号：US5871499A

    公开(公告)日：1999-02-16

    申请号：US842985

    申请日：1997-04-25

    申请人： Soonkap Hahn ,  John Merritt ,  Pat Roycroft ,  Steven T. Saville

    发明人： Soonkap Hahn ,  John Merritt ,  Pat Roycroft ,  Steven T. Saville

    IPC分类号： A61B17/135 ,  A61B17/42

    CPC分类号： A61B17/42 ,  A61B17/1355

    摘要： The childbirth-assisting device uses an automatically synchronized expandable pneumatic girdle to externally augment the secondary force of labor. The girdle through which the pressure is applied is fitted around the abdomen of the woman. An inflatable bladder within the girdle is inflated to create a downward pressure on the abdomen upon detection of a contraction. An external pressure monitor is used to detect contractions to provide a signal for synchronizing inflation of the girdle. The external pressure monitor includes a solid state force sensor embedded within a pliable housing which is conformable to the patient's abdomen to improve accuracy.

    摘要翻译： 分娩辅助装置使用自动同步的可膨胀气动腰带来外部增加第二劳动力。 施加压力的腰带围绕着女人的腹部。 腰围内的充气气囊膨胀，以便在检测到收缩时在腹部产生向下的压力。 外部压力监视器用于检测收缩以提供用于同步腰带充气的信号。 外部压力监视器包括固定力传感器，其嵌入柔性外壳内，该固体力传感器符合患者的腹部以提高精度。

    BIODEGRADABLE THERMORESPONSIVE 3-ARM POLYETHYLENE GLYCOL POLY(LACTIDE-CO-GLYCOLIDE) COPOLYMER FOR GINSENG ADMINISTRATION
    10.
    发明申请
    BIODEGRADABLE THERMORESPONSIVE 3-ARM POLYETHYLENE GLYCOL POLY(LACTIDE-CO-GLYCOLIDE) COPOLYMER FOR GINSENG ADMINISTRATION 审中-公开
    可生物降解的三聚氰胺聚酰胺（LACTIDE-CO-GLYCOLIDE）三羟甲基丙烷三羟甲基丙烷共聚物

    公开(公告)号：US20110070320A1

    公开(公告)日：2011-03-24

    申请号：US12779026

    申请日：2010-05-12

    申请人： Soonkap Hahn ,  Weijian Zhang ,  Jae Young Yang ,  Younjung Yuk

    发明人： Soonkap Hahn ,  Weijian Zhang ,  Jae Young Yang ,  Younjung Yuk

    IPC分类号： A61K36/258 ,  A61P1/04

    CPC分类号： A61K36/258 ,  A61K9/0043 ,  A61K9/006

    摘要： The present invention discloses a composition comprising a ginseng product, a mucoadhesive and a mucosa penetration enhancer in an aqueous biodegradable block copolymer solution consisting of a 3-arm polyethylene glycol (PEG) and 3 polyesters chains, each of which extends on each of the arm of the 3-arm PEG. The invention also provides a method for transmucosal delivery of pharmaceuticals and nutraceutical such as ginsenosides with improved absorption and bioavailability.

    摘要翻译： 本发明公开了一种组合物，其包含由3-臂聚乙二醇（PEG）和3个聚酯链组成的水性生物可降解嵌段共聚物溶液中的人参制品，粘膜粘附剂和粘膜渗透促进剂，其中每一个在每个臂上延伸 的3臂PEG。 本发明还提供了具有改善的吸收和生物利用度的药物和营养药物如人参皂苷的经粘膜递送的方法。