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    Method for producing segmental aneuploidy (SAN) strains of Trichoderma reesei via sexual crossing and SAN strains produced therefrom
    95.
    发明授权
    Method for producing segmental aneuploidy (SAN) strains of Trichoderma reesei via sexual crossing and SAN strains produced therefrom 有权

    公开(公告)号：US09598738B2

    公开(公告)日：2017-03-21

    申请号：US15027032

    申请日：2014-10-01

    Applicant: Academia Sinica

    Inventor： Ting-Fang Wang

    IPC: C12N1/15 ,  C12Q1/68 ,  C12N1/14

    CPC classification number: C12Q1/6895 ,  C12N1/14

    Abstract: The present invention relates to a technology to provide segmental aneuploidy progeny strains of Trichoderma reesei. In particular, the present invention relates to a method to produce segmental aneuploidy progeny strains of Trichoderma reesei via sexual crossing of two parent haploid strains with chromosome heterozygosity (e.g. one having scaffold M and scaffold 33, the other having scaffold F and scaffold X), preferably at least one of which includes a non-homologous end joining (NHEJ) gene. The present invention also relates to stable, segmental aneuploidy progeny strains of richoderma reesei thus produced which particularly exhibit enhanced gene expression or activities of carbohydrate-active enzymes (CAZymes) and more particularly prevent returning to euploidy for an extended period of time.

    METHOD FOR HIGH EFFICIENCY PROTEIN DELIVERY INTO PLASTIDS
    97.
    发明申请
    METHOD FOR HIGH EFFICIENCY PROTEIN DELIVERY INTO PLASTIDS 审中-公开
    高效蛋白质输送到塑料中的方法

    公开(公告)号：US20170016011A1

    公开(公告)日：2017-01-19

    申请号：US15210445

    申请日：2016-07-14

    Applicant: ACADEMIA SINICA

    Inventor： Hsou-Min Li ,  Chiung-Chih Chu

    IPC: C12N15/82

    CPC classification number: C12N15/8214 ,  C12N15/8221

    Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.

    Abstract translation: 本发明提供了编码融合蛋白的重组DNA分子，其包含编码与编码乘客蛋白质的第二DNA序列可操作地连接的高效转运肽的第一DNA序列，其中所述高效转运肽选自 叶绿体内包膜40kD（prTic40），伴侣蛋白10-2（prCpn10-2），纤维蛋白1A（prFibrillin），ATP硫化酶1（prAPS1），ATP硫化酶3（prAPS3））的转运蛋白前体的转运肽， ，5'-腺苷酸还原酶3（prAPR3），基质抗坏血酸过氧化物酶（prsAPX），prTic40-E2A（prTic40变体），prCpn10-1-ΔC7C37S（伴侣蛋白10-1变体），任何转运肽的功能片段 及其等同物。 并且本发明还提供了使用高效转运肽将蛋白质高效递送到质体中的方法。

    Collection and concentration system for biologic substance of interest and use thereof
    98.
    发明授权
    Collection and concentration system for biologic substance of interest and use thereof 有权
    生物物质的收集和浓缩系统及其用途

    公开(公告)号：US09494500B2

    公开(公告)日：2016-11-15

    申请号：US14065265

    申请日：2013-10-28

    Applicant: Academia Sinica

    Inventor： Ying-Chih Chang ,  Chia-Hsien Hsu

    IPC: C12M1/00 ,  G01N1/40 ,  B01L3/00 ,  B01D61/18 ,  B01D63/08

    CPC classification number: G01N1/405 ,  B01D61/18 ,  B01D63/088 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/027 ,  B01L2300/0681 ,  B01L2300/163

    Abstract: A system and method thereof for collecting and concentrating a biologic substance of interest is provided. The biologic of interest obtained from a biologic sample present at an initial low concentration (or low number counts) can be captured and released through a collection device of the system to an intermediate second concentration, and further recovered through a concentration device of the system to a third concentration, thereby facilitating subsequent detection, characterization, enumeration, immunostaining, inspection, imaging, culturing, molecular analysis, and/or other assays.

    Abstract translation: 提供了一种用于收集和浓缩感兴趣的生物物质的系统及其方法。 从初始低浓度（或低数量计数）存在的生物样品获得的感兴趣的生物学物质可以通过系统的收集装置捕获并释放到中间第二浓度，并通过系统的浓度装置进一步回收至 从而促进随后的检测，表征，计数，免疫染色，检查，成像，培养，分子分析和/或其他测定。

    TILAPIA PISCIDINS FOR USE IN ENHANCEMENT OF WOUND HEALING
    99.
    发明申请
    TILAPIA PISCIDINS FOR USE IN ENHANCEMENT OF WOUND HEALING 有权
    用于治疗伤口愈合的利多卡因

    公开(公告)号：US20160310564A1

    公开(公告)日：2016-10-27

    申请号：US15137281

    申请日：2016-04-25

    Applicant: Academia Sinica ,  National Taiwan Ocean University

    Inventor： Jyh-Yih CHEN ,  Chang-Jer WU

    IPC: A61K38/17

    CPC classification number: A61K38/1706

    Abstract: The preset invention relates to a new method for enhancing wound healing comprising administering a subject in need thereof with a therapeutically effective amount of a tilapia piscidin (TP), which is selected from the group consisting of TP1, TP2, TP3, TP4, TP5 and combination thereof. A composition or pharmaceutical composition comprising the TP are also provided.

    Abstract translation: 本发明涉及一种用于增强伤口愈合的新方法，包括给予有需要的受试者治疗有效量的选自TP1，TP2，TP3，TP4，TP5和 的组合。 还提供了包含TP的组合物或药物组合物。

    METHODS FOR FULL-LENGTH AMPLIFICATION OF DOUBLE-STRANDED LINEAR NUCLEIC ACIDS OF UNKNOWN SEQUENCES
    100.
    发明申请
    METHODS FOR FULL-LENGTH AMPLIFICATION OF DOUBLE-STRANDED LINEAR NUCLEIC ACIDS OF UNKNOWN SEQUENCES 审中-公开
    全长度放大双向线性核酸的未知序列的方法

    公开(公告)号：US20160298172A1

    公开(公告)日：2016-10-13

    申请号：US15037322

    申请日：2014-12-14

    Applicant: ACADEMIA SINICA

    Inventor： Kuo Ping CHIU ,  Yu-Shin NAI

    IPC: C12Q1/68 ,  G06F19/22 ,  C40B30/02

    CPC classification number: C12Q1/686 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6886 ,  C12Q2600/156 ,  G16B30/00 ,  G16B35/00 ,  G16C20/60 ,  C12Q2521/501 ,  C12Q2525/155 ,  C12Q2525/173 ,  C12Q2525/191 ,  C12Q2531/113 ,  C12Q2535/122

    Abstract: An adaptor for use in amplifying all linear, double-stranded nucleic acid molecules of unknown sequences in a sample is disclosed. The adaptor consists of: (1) the first oligonucleotide (P-oligo) with a phosphate at the 5′ end and without an additional thymine nucleotide at the 3′ end; and (2) the second oligonucleotide (T˜oligo) with an extra 3′-T and without a 5′-phosphate. The P-oligo and T-oligo are complementary to each other except at the 3′-T (thymine) in the T-oligo. The adaptor is ligated to nucleic acids of unknown sequences which have an extra A in the 3′ end (3′˜A overhang) to form adaptor-ligated target nucleic acids. The T-oligo is then employed as a single primer for T-oligo-primed polymerase chain reaction (TOP-PCR) and amplifies the nucleic acids of unknown sequences in full-length.

    Abstract translation: 公开了用于扩增样品中未知序列的所有线性双链核酸分子的衔接子。 衔接头由以下部分组成：（1）在5'末端具有磷酸酯的第一寡核苷酸（P-oligo），在3'端没有另外的胸腺嘧啶核苷酸; 和（2）具有额外的3'-T并且没有5'-磷酸的第二寡核苷酸（T〜ol）。 除了在T-oligo中的3'-T（胸腺嘧啶）之外，P-寡聚和T-寡聚是互补的。 将衔接子连接到在3'末端具有额外A（3'-A突出端）的未知序列的核酸以形成衔接物连接的靶核酸。 然后将T-oligo用作T-oligo-primed聚合酶链反应（TOP-PCR）的单引物，并扩增全长未知序列的核酸。

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