公开(公告)号：US10023875B2

公开(公告)日：2018-07-17

申请号：US14418659

申请日：2013-07-30

Applicant: BASF Plant Science Company GmbH

Inventor： Holger Schultheiss ,  Ralf Flachmann

IPC: C12N15/82 ,  C07K14/415

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the order Pucciniales, preferably the family Phacopsoraceae, in plants and/or plant cells. This is achieved by increasing the expression of an HCP5 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the order Pucciniales, preferably the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP5 protein.

公开(公告)号：US09879234B2

公开(公告)日：2018-01-30

申请号：US14416981

申请日：2013-07-30

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Laurent Marty ,  Toralf Senger

IPC: C12N9/00 ,  C12P7/64 ,  C12N9/02

CPC classification number: C12N9/0071 ,  C12N9/001 ,  C12P7/6427 ,  C12Y114/19

Abstract: The invention provides nucleic acid molecules which encodes a novel fatty acid desaturase, KCS, KCR and/or LACS from Thraustochytrium aureum and Sphaeroforma arctica. The invention also provides recombinant expression vectors containing the nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA and for screening for delta-4 desaturases.

公开(公告)号：US20160272951A1

公开(公告)日：2016-09-22

申请号：US15159931

申请日：2016-05-20

Applicant: BASF Plant Science Company GmbH

Inventor： Toralf Senger ,  Laurent MARTY ,  Sten STYMNE ,  Jenny LINDBERG YILMAZ ,  Johnathan A. NAPIER ,  Olga SAYANOVA ,  Richard HASLAM ,  Ruiz Lopez NOEMI

IPC: C12N9/10 ,  A23K20/158 ,  A23D9/02 ,  C12P7/64 ,  C12N15/82

CPC classification number: C11C1/045 ,  A23D9/02 ,  A23K20/158 ,  A23L33/105 ,  A23L33/115 ,  A23L33/12 ,  A23V2002/00 ,  A23V2250/1862 ,  A23V2250/1868 ,  A23V2250/187 ,  A23V2250/1882 ,  C12N15/8247 ,  C12P7/6427 ,  C12Y203/0102

Abstract: The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

Abstract translation: 本发明涉及多不饱和脂肪酸的重组制造。 具体地，本发明涉及酰基转移酶多肽，编码所述酰基转移酶的多核苷酸以及含有所述多核苷酸的载体，宿主细胞，非人转基因生物。 此外，本发明考虑了制备多不饱和脂肪酸的方法以及通过这些方法获得的油。

公开(公告)号：US20150361443A1

公开(公告)日：2015-12-17

申请号：US14763659

申请日：2014-01-10

Applicant: BASF Plant Science Company GmbH

Inventor： Holger Schultheiss ,  Ralf Flachmann

IPC: C12N15/82

CPC classification number: C12N15/8282 ,  A01H5/10 ,  C07K14/415 ,  C12N15/8216

Abstract: The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacosporaceae in plants and/or plant cells. This is achieved by increasing the expression of an HCP6 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP6 protein.

Abstract translation: 本发明涉及在植物和/或植物细胞中增加对孢子科家族的真菌病原体的抗性的方法。 与野生型植物，野生型植物部分和/或野生型植物细胞相比，通过增加植物，植物部分和/或植物细胞中HCP6蛋白或其片段的表达来实现。 此外，本发明涉及具有增加的对真菌病原体，特别是Phacopsoraceae科的病原体的抗性的转基因植物，和/或植物细胞，以及包含与序列相同或同源的序列的重组表达载体 编码HCP6蛋白。

公开(公告)号：US20150268156A1

公开(公告)日：2015-09-24

申请号：US14730531

申请日：2015-06-04

Applicant: BASF Plant Science Company GmbH

Inventor： Frederik Leyns ,  Cédrick VANDAELE ,  Pierre LEJEUNE ,  Jeroen BAERT ,  Fabio FIORANI

IPC: G01N21/17 ,  G01N21/84 ,  G01N33/00 ,  A01G1/00

CPC classification number: G01N21/17 ,  A01G7/00 ,  A01G22/00 ,  G01N21/84 ,  G01N33/0098 ,  G01N2021/1765 ,  G01N2021/8466

Abstract: A screening device (110) for screening at least one plant specimen (112) in a plurality of plant specimens (114) is disclosed. The screening device (110) comprises a detector (116) adapted for acquiring spatially resolved images (117). The screening device (110) further comprises at least one selection device (118) adapted for selecting a single plant specimen (120) or a group of plant specimens (122) from the plurality of plant specimens (114) for imaging by the detector (116). The selection device (118) comprises a deflection device (124) adapted for deflecting electromagnetic waves propagating between the plant specimens (112) and the detector (116).

Abstract translation: 公开了一种用于筛选多个植物试样（114）中的至少一个植物试样（112）的筛选装置（110）。 筛选装置（110）包括适于获取空间分辨图像（117）的检测器（116）。 筛选装置（110）还包括至少一个选择装置（118），适于从多个植物样本（114）中选择单个植物标本（120）或一组植物标本（122），以便由检测器 116）。 选择装置（118）包括适于偏转在植物样本（112）和检测器（116）之间传播的电磁波的偏转装置（124）。

公开(公告)号：US09139854B2

公开(公告)日：2015-09-22

申请号：US14338673

申请日：2014-07-23

Applicant: BASF Plant Science Company GmbH

Inventor： Toralf Senger ,  Jörg Bauer ,  Johnathan A. Napier

IPC: C12P7/64 ,  C12N9/02 ,  C07H21/02 ,  C12N15/82

CPC classification number: C12P7/6409 ,  C12N9/0071 ,  C12N9/0083 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  C12P7/6481 ,  C12Y114/19001

Abstract: The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerin and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.

Abstract translation: 本发明涉及来自嗜球菌（Cocchioiobolus heterostrophus）C5，蓝藻（Cyanothece sp。）的多核苷酸 CCY0110，真菌孢子菌和寄生藻类，代表去饱和酶，可用于重组生产多不饱和脂肪酸。 本发明还涉及包含根据本发明的多核苷酸的载体，宿主细胞和转基因非人生物以及由多核苷酸编码的多肽。 本发明还涉及针对根据本发明的多肽的抗体。 最后，本发明还涉及多不饱和脂肪酸和油，脂和脂肪酸组合物的生产方法以及它们作为药物，化妆品，食品，饲料，优选鱼食或食品添加剂的用途。

公开(公告)号：US20150106974A1

公开(公告)日：2015-04-16

申请号：US14581334

申请日：2014-12-23

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： VALERIE FRANKARD ,  ANDRY ANDRIANKAJA ,  YVES HATZFELD ,  MARIEKE LOUWERS ,  STEVEN VANDENABEELE ,  AURINE VERKEST ,  GEERT DE JAEGER ,  DIRK INZÉ

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C12N15/8216 ,  Y02A40/146

Abstract: The present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of one ore more nucleic acid(s) encoding at least two iSYT (interactor of SYT—synovial sarcoma translocation—) polypeptides. The present invention also concerns plants having modulated expression of a nucleic acid encoding at least two iSYT polypeptides, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. Nucleic acids encoding at least two iSYT polypeptides and constructs comprising the same useful in performing the methods of the invention are also disclosed. Also provided are constructs useful in the methods of the invention. The present invention also relates to an iSYT-based protein complex. The use of the complex to promote plant growth, and a method for stimulating the complex formation, by overexpressing at least two members of the complex, are also disclosed.

Abstract translation: 本发明涉及通过调节编码至少两种iSYT（SYT-滑膜肉瘤易位 - 相互作用 - ）多肽的相互作用的一个或多个核酸的植物中的表达来增强植物中产量相关性状的方法。 本发明还涉及具有编码至少两种iSYT多肽的核酸的调节表达的植物，该植物相对于相应的野生型植物或其它对照植物具有增强的产量相关性状。 还公开了编码至少两种iSYT多肽的核酸和包含与用于实施本发明的方法相同的构建体的构建体。 还提供了可用于本发明方法的构建体。 本发明还涉及基于iSYT的蛋白质复合物。 还公开了复合物促进植物生长的用途，以及通过过表达复合物的至少两个成员刺激复合物形成的方法。

公开(公告)号：US20150082492A1

公开(公告)日：2015-03-19

申请号：US14389896

申请日：2013-03-15

Applicant: BASF Plant Science Company GmbH

Inventor： Christophe Reuzeau

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C07K14/195 ,  C12N15/8271 ,  C12N15/8273 ,  Y02A40/146

Abstract: A method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a flavodoxin polypeptide in a specific way is provided and unknown constructs useful in performing the method are provided. Plants having the expression of a nucleic acid encoding a flavodoxin polypeptide modulated by a particular type of promoter, which plants have enhanced yield-related traits compared with control plants, are also provided.

Abstract translation: 提供了通过以特定方式调节植物中编码黄素氧还蛋白多肽的核酸的表达来提高植物中产量相关性状的方法，并提供了用于实施该方法的未知构建体。 还提供了具有编码由特定类型的启动子调节的黄素氧还蛋白多肽的核酸表达的植物，该植物与对照植物相比具有增强的产量相关性状。

公开(公告)号：US20150059735A1

公开(公告)日：2015-03-05

申请号：US14389917

申请日：2013-03-15

Applicant: BASF Plant Science Company GmbH

Inventor： Christophe Reuzeau

IPC: C12N15/82 ,  C13B10/00

CPC classification number: C12N15/8201 ,  C07K14/195 ,  C12N15/8261 ,  C12N15/8273 ,  C13B10/00 ,  Y02A40/146

Abstract: A method for enhancing various economically important yield-related traits in plants by modulating expression of a nucleic acid encoding a flavodoxin polypeptide in plants in a specific way. Provided are plants having the expression of a nucleic acid encoding a flavodoxin polypeptide modulated by a particular type of promoter, which plants have enhanced yield-related traits compared with control plants. Hitherto unknown constructs, which are useful in performing the methods of the invention, are also provided.

Abstract translation: 通过以特定方式调节植物中编码黄素氧还蛋白多肽的核酸的表达来增强植物中各种经济上重要的产量相关性状的方法。 提供了具有编码由特定类型的启动子调节的黄素氧还蛋白多肽的核酸的表达的植物，与对照植物相比，该植物具有增强的产量相关性状。 还提供了迄今未知的构造，其在实施本发明的方法中是有用的。

公开(公告)号：US20140304858A1

公开(公告)日：2014-10-09

申请号：US14352793

申请日：2012-10-12

Applicant: BASF Plant Science Company GmbH

Inventor： Ana Isabel Sanz Molinero ,  Yves Hatzfeld

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C07K14/415 ,  Y02A40/146

Abstract: Provided is a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a HhH-GPD-related polypeptide, or a calnexin-related polypeptide. Also provided are plants having modulated expression of a nucleic acid encoding a HhH-GPD-related polypeptide, or a calnexin-related polypeptide, which plants have enhanced yield-related traits relative to control plants.

Abstract translation: 提供了一种通过调节植物中编码HhH-GPD相关多肽或钙血清蛋白相关多肽的核酸表达来增强植物中产量相关性状的方法。 还提供了具有调节表达编码HhH-GPD相关多肽或钙血清蛋白相关多肽的核酸的植物，该植物相对于对照植物具有增强的产量相关性状。