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公开(公告)号：US11278881B2
公开(公告)日：2022-03-22
申请号：US17387990
申请日：2021-07-28
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Chueh-Yu Wu , Ghulam Destgeer , Mengxing Ouyang
IPC: B01L3/02 , B01L3/00 , G01N33/543 , C12N1/04 , C12N5/071 , C12N5/0783 , C12Q1/6806
Abstract: Sub-millimeter scale three-dimensional (3D) structures are disclosed with customizable chemical properties and/or functionality. The 3D structures are referred to as drop-carrier particles. The drop-carrier particles allow the selective association of one solution (i.e., a dispersed phased) with an interior portion of each of the drop-carrier particles, while a second non-miscible solution (i.e., a continuous phase) associates with an exterior portion of each of the drop-carrier particles due to the specific chemical and/or physical properties of the interior and exterior regions of the drop-carrier particles. The combined drop-carrier particle with the dispersed phase contained therein is referred to as a particle-drop. The selective association results in compartmentalization of the dispersed phase solution into sub-microliter-sized volumes contained in the drop-carrier particles. The compartmentalized volumes can be used for single-molecule assays as well as single-cell, and other single-entity assays.
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92.发明授权公开(公告)号：US11175280B2
公开(公告)日：2021-11-16
申请号：US17033488
申请日：2020-09-25
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Peter Tseng , Ivan Pushkarsky
Abstract: A system for assaying forces applied by cells includes an optically transparent substrate comprising a soft material having a Young's modulus within the range of about 3 kPa to about 100 kPa. An array of molecular patterns is disposed on a surface of the optically transparent substrate, the molecular patterns include fluorophore-conjugated patterns adherent to cells. The system includes at least one light source configured to excite the fluorophore-conjugated patterns and an imaging device configured to capture fluorescent light emitted from the fluorophore-conjugated patterns. Dimensional changes in the size of the patterns are used to determine contractile forces imparted by cells located on the patterns.
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93.发明授权公开(公告)号：US11060541B2
公开(公告)日：2021-07-13
申请号：US15763765
申请日：2016-09-30
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Chueh-Yu Wu
Abstract: A method of forming three-dimensional shaped microparticles in a microfluidic device includes flowing a mixture of a monomer and photoinitiator in a microfluidic channel having a plurality of pillars disposed therein to define a flow stream having a pre-defined shape and temporarily stopping the same. One or more portions of the flow stream are polymerized by passing polymerizing light through one or more masks and onto the flow stream, the polymerization process forming a plurality of three-dimensional shaped microparticles. The three-dimensional shape of the microparticle may be geometrically complex by using non-rectangular 2D orthogonal shapes for the flow and/or masked light source. The microparticles may include protected regions on which cells can be adhered to and protected from shear forces. The flow stream is restarted to flush out the newly formed microparticles and prepare the device for the next cycle of particle formation.
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公开(公告)号：US10969327B2
公开(公告)日：2021-04-06
申请号：US16374663
申请日：2019-04-03
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Daniel R. Gossett , Henry T. K. Tse , Aram Chung
Abstract: A system for deforming and analyzing a plurality of particles carried in a sample volume includes a substrate defining an inlet, configured to receive the sample volume, and an outlet; and a fluidic pathway fluidly coupled to the inlet and the outlet. The fluidic pathway includes a delivery region configured to receive the plurality of particles from the inlet and focus the plurality of particles from a random distribution to a focused state, a deformation region defining an intersection located downstream of the delivery region and coupled to the outlet, and wherein the deformation region is configured to receive the plurality of particles from the delivery region and to transmit each particle in the plurality of particles into the intersection from a single direction, a first branch fluidly coupled to the deformation region and configured to transmit a first flow into the intersection, and a second branch fluidly coupled to the deformation region and configured to transmit a second flow, substantially opposing the first flow, into the intersection, wherein the first flow and the second flow are configured to induce extension of one or more particles in the plurality of particles.
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公开(公告)号：US10690290B2
公开(公告)日：2020-06-23
申请号：US16276974
申请日：2019-02-15
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Wonhee Lee
Abstract: A particle focusing system includes an inlet; an inertial focusing microchannel disposed in a substrate and connected to the inlet; and a pressure/flow source configured to drive a particle-containing fluid through the inertial focusing microchannel, where the inertial focusing microchannel includes a side wall having an irregular surface. The side wall includes a first irregularity protruding from a baseline surface away from a longitudinal axis of the inertial focusing microchannel. Alternatively or additionally, the first irregularity and the baseline surface form an angle more than or equal to 135 degrees. The inertial focusing microchannel may have a substantially rectangular cross-section having a height and a width, and a ratio of height to width is approximately 5:4 to 4:1. The system may also include a downstream expanding region having a side wall, where the side wall has a stepped surface.
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Patent Agency Ranking
96.发明申请公开(公告)号：US20200025745A1
公开(公告)日：2020-01-23
申请号：US16584419
申请日：2019-09-26
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Peter Tseng , Ivan Pushkarsky
Abstract: A system for assaying forces applied by cells includes an optically transparent substrate comprising a soft material having a Young's modulus within the range of about 3 kPa to about 100 kPa. An array of molecular patterns is disposed on a surface of the optically transparent substrate, the molecular patterns include fluorophore-conjugated patterns adherent to cells. The system includes at least one light source configured to excite the fluorophore-conjugated patterns and an imaging device configured to capture fluorescent light emitted from the fluorophore-conjugated patterns. Dimensional changes in the size of the patterns are used to determine contractile forces imparted by cells located on the patterns.
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公开(公告)号：US20190178449A1
公开(公告)日：2019-06-13
申请号：US16276974
申请日：2019-02-15
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Wonhee Lee
CPC classification number: F17D1/00 , G01N15/1404 , G01N2015/1415 , Y10T137/85978
Abstract: A particle focusing system includes an inlet; an inertial focusing microchannel disposed in a substrate and connected to the inlet; and a pressure/flow source configured to drive a particle-containing fluid through the inertial focusing microchannel, where the inertial focusing microchannel includes a side wall having an irregular surface. The side wall includes a first irregularity protruding from a baseline surface away from a longitudinal axis of the inertial focusing microchannel. Alternatively or additionally, the first irregularity and the baseline surface form an angle more than or equal to 135 degrees. The inertial focusing microchannel may have a substantially rectangular cross-section having a height and a width, and a ratio of height to width is approximately 5:4 to 4:1. The system may also include a downstream expanding region having a side wall, where the side wall has a stepped surface.
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98.发明授权公开(公告)号：US10226769B2
公开(公告)日：2019-03-12
申请号：US15785191
申请日：2017-10-16
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Daniel R. Gossett , Henry T. K. Tse
Abstract: A method of exchanging fluids with suspended particles includes providing a microfluidic device with a first inlet channel operatively coupled to a source of particles and a second inlet channel operatively coupled to an exchange fluid. A transfer channel is connected at a proximal end to the first inlet channel and the second inlet channel. First and second outlet channels are connected to a distal end of the transfer channel. The source of particles is flowed at a first flow rate into the first inlet channel while the exchange fluid is flowed at a second flow rate into the second inlet channel wherein the ratio of the second flow rate to the first flow rate is at least 1.5. Particles are collected in one of the first and second outlet channels while fluid substantially free of particles is collected in the other of the first and second outlet channels.
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公开(公告)号：US10107735B2
公开(公告)日：2018-10-23
申请号：US15377659
申请日：2016-12-13
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Daniel R. Gossett , Henry T. K. Tse , Aram Chung
Abstract: A system for deforming and analyzing particles includes a substrate defining an inlet, and an outlet; a fluidic pathway fluidly coupled to the inlet and the outlet and defining a delivery region upstream of a deformation region configured to deform particles, wherein the fluidic pathway comprises a first branch configured to generate a first flow, and a second branch configured to generate a second flow that opposes the first flow, wherein an intersection of the first flow and the second flow defines the deformation region; a detection module including a sensor configured to generate a morphology dataset characterizing deformation of the particles, and a photodetector configured to generate a fluorescence dataset characterizing fluorescence of the particles; and a processor configured to output an analysis of the plurality of particles based at least in part on the deformation dataset and the fluorescent dataset for the plurality of particles.
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公开(公告)号：US20180296992A1
公开(公告)日：2018-10-18
申请号：US15767979
申请日：2016-10-07
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Dino Di Carlo , Soroush Kahkeshani
CPC classification number: B01F3/0815 , A61K9/0009 , A61K9/0021 , A61K9/0024 , A61K9/0097 , A61M2037/0023 , B01F13/0059 , B01F13/0077 , B01L3/502761 , B01L3/502784 , B01L2200/0668 , B01L2300/0816 , B01L2300/0867 , B01L2400/043 , G01N35/1002 , G01N2035/1034
Abstract: A microfluidic device for forming droplets includes at least one ferrofluid reservoir disposed in the microfluidic device and containing a ferrofluid therein. The microfluidic device includes a continuous-phase reservoir disposed in the microfluidic device and containing an oil phase therein and one or more microfluidic channels connecting between the at least one ferrofluid reservoir and the continuous-phase reservoir, the continuous-phase reservoir comprising a step region having an increased height as compared to a height of the one or more microfluidic channels. To form droplets an externally applied magnetic field is applied to the device to pull the ferrofluid into the continuous-phase reservoir, whereby droplets are formed at step region.
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