公开(公告)号：US09713501B2

公开(公告)日：2017-07-25

申请号：US14325908

申请日：2014-07-08

Applicant: Wake Forest University Health Sciences ,  North Carolina State University

Inventor： Rohan A. Shirwaiker ,  Caroline Elise Webster ,  John D. Jackson ,  Richard A. Wysk ,  Sang Jin Lee ,  Ola L. A. Harrysson ,  James J. Yoo ,  Anthony Atala ,  Paul H. Cohen ,  Yuan-Shin Lee ,  Peter M. Prim ,  Molly F. Purser ,  Katie L. Basinger

IPC: A61B17/322 ,  A61B19/00 ,  A61B90/00

CPC classification number: A61B90/02 ,  A61B17/322 ,  A61B2017/3225

Abstract: Provided herein is a tissue stretching device configured for orbicular expansion of a tissue placed therein. Methods of use of the device to stretch a tissue as well as for culturing organized tissues are also provided. Stretched and/or cultured tissues produced by these processes are also provided, as well as methods making use of the same.

公开(公告)号：US20160296667A1

公开(公告)日：2016-10-13

申请号：US15159219

申请日：2016-05-19

Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

Inventor： Anthony Atala ,  James Yoo ,  In Kap Ko

IPC: A61L27/36 ,  A61L27/24 ,  C12N5/00 ,  A61L27/54 ,  C12N5/077 ,  A61L27/38 ,  A61L27/18

CPC classification number: A61L27/3604 ,  A61K9/0034 ,  A61K35/34 ,  A61K38/18 ,  A61K45/06 ,  A61K47/34 ,  A61K47/42 ,  A61L27/18 ,  A61L27/24 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3834 ,  A61L27/54 ,  A61L2300/414 ,  A61L2400/06 ,  A61L2430/30 ,  C12N5/0062 ,  C12N5/0659 ,  C12N2513/00 ,  C12N2533/30 ,  C12N2533/54 ,  A61K2300/00 ,  C08L67/04

Abstract: The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.

Abstract translation: 本发明涉及用于获得用于移植的均匀尺寸的肌纤维片段的方法和组合物。 这些肌纤维碎片能够重建成沿着原生肌肉定向的长纤维。 植入的肌肉细胞与天然血管和神经网络结合，如组织学和免疫组织化学所证实的。 本发明是特别有利的，因为可以从供体部位收获自体肌肉，将其加工并注射到手术室中的靶位点。 碎片肌肉纤维可以容易地集成在宿主内。

公开(公告)号：US20160199179A1

公开(公告)日：2016-07-14

申请号：US14987601

申请日：2016-01-04

Applicant: Wake Forest University Health Sciences

Inventor： Anthony Atala ,  James Yoo

IPC: A61F2/24 ,  C12N5/00 ,  C12N5/071

CPC classification number: A61F2/2415 ,  A61L27/3604 ,  A61L27/3641 ,  A61L27/3808 ,  A61L27/3826 ,  A61L27/383 ,  A61L27/3839 ,  A61L27/3886 ,  A61L27/507 ,  B82Y5/00 ,  B82Y10/00 ,  C12N5/0068 ,  C12N5/069 ,  C12N2533/90

Abstract: The invention is directed to methods for preparing artificial heart valves by preconditioning a matrix seeded with endothelial cells and smooth muscle cells differentiated from isolated progenitor cells. These cell seeded matrices are exposed to fluid conditions that mimic blood flow through the heart to produce tissue engineered heart valves that are analogous to native heart valves.

Abstract translation: 本发明涉及通过预处理由分离的祖细胞分化的内皮细胞和平滑肌细胞的基质来制备人造心脏瓣膜的方法。 这些细胞接种的基质暴露于模拟通过心脏的血液流动以产生类似于天然心脏瓣膜的组织工程心脏瓣膜的流体条件。

公开(公告)号：US20150299730A1

公开(公告)日：2015-10-22

申请号：US14697704

申请日：2015-04-28

Applicant: Wake Forest University Health Sciences

Inventor： Tao Xu ,  James J. Yoo ,  Anthony Atala

IPC: C12N15/89 ,  A61K31/713

CPC classification number: C12N15/895 ,  A61K31/713 ,  C12N15/63 ,  C12N15/87 ,  C12N15/89

Abstract: Provided herein are methods and apparatuses for transfecting a cell with a compound of interest by stressing the cell, e.g. with shear stress. The compound of interest may be nucleic acids, proteins, molecules, nanoparticles, drugs, etc., or any combination thereof. Methods of printing cells with an inkjet printing device are also provided, wherein at least a portion of viable cells (preferably at least 1%) are transfected with a compound of interest. Preferably, at least 25% of the cells are viable after printing. In addition, methods of forming an array of viable cells are provided wherein at least a portion of the viable printed cells (preferably at least 1%) are transfected with at least one compound of interest.

Abstract translation: 本文提供了用于通过施加细胞来转染具有感兴趣的化合物的细胞的方法和装置，例如， 具有剪切应力。 感兴趣的化合物可以是核酸，蛋白质，分子，纳米颗粒，药物等，或其任何组合。 还提供了用喷墨印刷装置印刷细胞的方法，其中至少一部分活细胞（优选至少1％）用感兴趣的化合物转染。 优选地，至少25％的细胞在印刷后是活的。 此外，提供形成活细胞阵列的方法，其中至少一部分活的印刷细胞（优选至少1％）用至少一种目标化合物转染。